BACKGROUND: Extended-spectrum beta-lactamase-producing (ESBL) Klebsiella pneumoniae is an important cause of nosocomial infections in neonatal intensive care units (NICUs). This study aimed to reduce ESBL-producing K. pneumoniae in the NICU by using infection control measures. METHODS: We performed prospective surveillance cultures, monitoring, and education for infection control in the NICU between May and August 2011. Specimens were collected from all infants and the environment including stethoscopes, thermometers, ventilators, incubators, etc. The anterior nares and hands of healthcare workers were also screened. We inspected infection control practices and provided feedback. The level of infection control awareness was measured using a questionnaire. RESULTS: The level of awareness and performance of hand washing increased significantly after intervention (both P<0.001). The environmental management of healthcare providers also improved significantly (P=0.001). The yield of ESBL-producing K. pneumoniae from clinical specimens decreased gradually throughout the study period (30.4% in May to 12.6% in August). Central catheter-related K. pneumoniae bacteremia decreased from 1.3/1000 to 0/1000 catheter-days. CONCLUSION: Infection control measures including education, monitoring, and surveillance can lower the incidence of ESBL-producing K. pneumoniae in the NICU.
Bacteremia ; beta-Lactamases ; Cross Infection ; Delivery of Health Care ; Hand ; Hand Disinfection ; Health Personnel ; Humans ; Incidence ; Incubators ; Infant ; Infant, Newborn ; Infection Control ; Intensive Care Units ; Intensive Care Units, Neonatal ; Intensive Care, Neonatal ; Klebsiella ; Klebsiella pneumoniae ; Pneumonia ; Prospective Studies ; Stethoscopes ; Thermometers ; Ventilators, Mechanical

Lung cancer is the most common malignant tumor worldwide and its incidence continues to rise each year. Recent development of molecular biologic method has led to advances in determining the etiologic factors of lung cancer and the establishment of cell lines has provided a lot of information on the through chemosensitivity, radiation biology studies, cytogenetics, and molecular biologic studies, which permits improved treatment for lung cancer. We established a small cell lung cancer cell line, designated JePa-1, obtained from malignant pericardial effusion of small cell lung cancer patient and characterized its morphologic and molecular biologic features. the JePa-1 cell line grew relatively slowly (doubling time 45hrs) as very loosely adherent floating aggregates growing in small clumps with distinct cell outlines and intertwined cords. Also JePa-1 cell line secreted antidiuretic hormones. Electronmicroscopic examination revealed that JePa-1 cell line and xenografts contained electron dense core granules, characteristic of being of neuroendocrine origin. To investigate the tumorigenic capacity, the JePa-1 cell line was injected into SCID and nude mice. Tumors taken from xenografts were observed in 3 out of 4 of the SCID mice and 2 out of 4 of the nude mice. The histologic characteristics of the xenografts were similar to those of the cell line and the original cytologic finding of the pericardial fluid, suggesting small cell carcinoma. The results of immunohistochemical markers showed reactivity for Rb protein, c-myc, TGF-alpha, TGF-beta , EGFR, keratin, NSE, chromogranin, and EMA. The DNA ploidy and the index of the JePa-1 cells was tetraploid and 2.13, respectively. The positive rate for the Rb, c-myc and K-ras proteins of the JePa-1 cell line were 98.9%, 99.3%, and 99.7% respectively as determined by flow cytometry. Cytogenetic analysis using the G-banding technique showed 65 chromosomes with various numerical and structural abnormalities. On examination of the expression of TGF-alpha, TGF-beta , and EGFR by PCR, only the EGFR was positive Through the establishment of JePa-1 cell line, we report in this paper the characterization of a small cell lung cancer such as morphologic and immunocytochemical features, growth characteristics in culture, hormone production, expression of oncoprotein and several growth factors, tumorigenicity, chromosomal abnormalities, and DNA ploidy and index. The JePa-1 cell line will be valuable in vitro studies for the etiology, treatment and the prognostic factors in small cell lung cancer.
Animals ; Carcinoma, Small Cell ; Cell Line ; Chromosome Aberrations ; Cytogenetic Analysis ; Cytogenetics ; DNA ; Flow Cytometry ; Heterografts ; Humans ; Immunohistochemistry ; Incidence ; Intercellular Signaling Peptides and Proteins ; Lung Neoplasms ; Mice ; Mice, Nude ; Mice, SCID ; Pericardial Effusion ; Ploidies ; Polymerase Chain Reaction ; Radiobiology ; Retinoblastoma Protein ; Small Cell Lung Carcinoma* ; Tetraploidy ; Transforming Growth Factor alpha ; Transforming Growth Factor beta ; Vasopressins

School Lunch Program (SLP) should provide adequate and qualitative nutritional food for student's growth and proper food habit. With this fact, this study divided 524 middle school students who resided in Jeonju, Jellabukdo into two groups, SLP or non SLP according to whether they were provided SLP or not. Then this research estimate each group's nutritional knowledge, nutritional attitude, dietary behavior, balance of three meals, and health checking their clinical symptom to investigate the effects of the SLP. This research applied anthropometric data, questionnaires and food intake data using 24 hours recall method. Each group's age average was same as 14.0 years old respectively. The average score of nutritional knowledge and condition of balance of lunch were significantly higher in SLP group than those of non-SLP group, although, there were no significant differences in the scores of nutritional attitude, dietary behavior, and balance of breakfast and dinner within both groups. This research showed that SLP had positive role on nutritional knowledge and balanced lunch. However, current SLP is not sufficient to cause change the nutritional attitude and dietary behavior. To make more applicable and effective result of SLP leading nutritional attitude changes and development of appropriate dietary behavior, certain level of nutritional education to the student may be important.
Breakfast ; Eating ; Education ; Food Habits ; Humans ; Jeollabuk-do ; Lunch* ; Meals ; Surveys and Questionnaires

Although the incidence of tuberculosis in Korea in general has declined in recent years, it remains an importat health problem, especially in children. Inspite of the recent controversy over the necessity of tuberculin skin test prior to measles vaccination, this brief report shows 13.8% of positive skin test at 9 and 15 months of age at the time of measles vaccination. We feel, in Korea, practicing tuberculin skin test prior to measles vaccination is good way of detecting asymptomatic infection in young age who needs preventive therapy.
Asymptomatic Infections ; Child ; Humans ; Incidence ; Korea ; Measles* ; Skin Tests* ; Skin* ; Tuberculin* ; Tuberculosis ; Vaccination*

No abstract available.
Autopsy* ; Hydrops Fetalis*

No abstract available.
Sertoli-Leydig Cell Tumor*

This study was performed to evaluate the effectiveness of music therapy as one of the psychiatric nursing intervention tools, with addtional information in relieving anxiety during the procedure. Data were collected through nonequivalent pre-and post tests from July 1, 1998 to September 30 1998 in 90 patients (test group A: 28 patients, test group B: 27 patients, control group: 33 patients) who were hospitalized in DongSan Medical Center in order to have cardiac catheterization. The Subjects were informed by educational videos, which were modified according to the sensory information of the 10 study patients. They were based on the informative booklet by Kim keum-soon (1989). The procedural information was also modified according to the hospital`s customs. Provided the music for patients suitable to their tastes, and measured their blood pressure, heart rate, the degree of anxiety using the Spielberger`s measurement device of anxiety, and behavioral response of Finesilver`s. The statistical significance was analyzed using chi-square test and ANOVA. The results of this study were as follows : Hypothesis 1 : There are significant differences in the degree of anxiety among test group A, Test group A was provided only information, Test group B was provided information and the control group was provided neither. Hypothesis 2 : There are significant differences in systolic blood pressure among test group A, test group B, and control group.: non-significant. Hypothesis 3 : There are significant differences in diastolic blood pressure among test group A, test group B, and control group.: significant(F=1.31, p=.27, interaction; F=3.80, p=.00). Hypothesis 4 : There are significant differences in heart rate among test group A, test group B, and control group.: non-significant. Hypothesis 5 : There are significant differences in behavioral responses among test group A, test group B, and control group.: significant(F=10.05, p=.00). Further validation study is required with other subjects and other settings.
Anxiety ; Blood Pressure ; Cardiac Catheterization ; Cardiac Catheters ; Coronary Artery Disease* ; Coronary Vessels* ; Heart Rate ; Humans ; Music Therapy ; Music* ; Pamphlets ; Psychiatric Nursing

This study was performed to evaluate the effectiveness of music therapy as one of the psychiatric nursing intervention tools, with addtional information in relieving anxiety during the procedure. Data were collected through nonequivalent pre-and post tests from July 1, 1998 to September 30 1998 in 90 patients (test group A: 28 patients, test group B: 27 patients, control group: 33 patients) who were hospitalized in DongSan Medical Center in order to have cardiac catheterization. The Subjects were informed by educational videos, which were modified according to the sensory information of the 10 study patients. They were based on the informative booklet by Kim keum-soon (1989). The procedural information was also modified according to the hospital`s customs. Provided the music for patients suitable to their tastes, and measured their blood pressure, heart rate, the degree of anxiety using the Spielberger`s measurement device of anxiety, and behavioral response of Finesilver`s. The statistical significance was analyzed using chi-square test and ANOVA. The results of this study were as follows : Hypothesis 1 : There are significant differences in the degree of anxiety among test group A, Test group A was provided only information, Test group B was provided information and the control group was provided neither. Hypothesis 2 : There are significant differences in systolic blood pressure among test group A, test group B, and control group.: non-significant. Hypothesis 3 : There are significant differences in diastolic blood pressure among test group A, test group B, and control group.: significant(F=1.31, p=.27, interaction; F=3.80, p=.00). Hypothesis 4 : There are significant differences in heart rate among test group A, test group B, and control group.: non-significant. Hypothesis 5 : There are significant differences in behavioral responses among test group A, test group B, and control group.: significant(F=10.05, p=.00). Further validation study is required with other subjects and other settings.
Anxiety ; Blood Pressure ; Cardiac Catheterization ; Cardiac Catheters ; Coronary Artery Disease* ; Coronary Vessels* ; Heart Rate ; Humans ; Music Therapy ; Music* ; Pamphlets ; Psychiatric Nursing

BACKGROUND: The genetic basis of isoniazid (INH) resistance in Mycobacterium tuberculosis has been attributed to at least four genes. Mutations of the katG gene encoding catalase-eroxidase have been shown to cause resistance to INH. Among these mutations, the point mutation of codons 315 and 463 are frequently found. To determine whether simple screening method could potentially be useful for the detection of INH-esistant strains, we investigated the presence of mutation of codons 315 and 463 of katG gene in INH-esistant M. tuberculosis from Korea. METHODS: We used the polymerase chain reaction (PCR) and direct sequencing analysis to detect the point mutation of codons 315 and 463 of katG gene in 48 strains of INH-esistant and 10 strains of INH-usceptible M. tuberculosis. RESULTS: No amplification product was generated from 2 of 48 INH-esistant strains. Among the remaining 46 isolates, point mutations at codons 315 and 463 were identified in 19 isolates (41.3%) and 40 isolates (87.0%), respectively. Among the 10 INH-usceptible strains, the codon 463 point mutation was identified in 7 isolates, however, no point mutation of the codon 315 was found. CONCLUSIONS: These results suggest that the point mutation of codon 463 of katG gene of M. tuberculosis may be a polymorphism not related with INH resistance. Although the mutation of codon 315 of katG gene was limited to 41.3% of INH-esistant isolates, further investigation of the codon 315 of katG gene should lead to increased understanding of resistance genes and the deveolpment of rapid molecular detection methods.
Codon ; Isoniazid ; Korea* ; Mass Screening ; Mycobacterium tuberculosis* ; Mycobacterium* ; Point Mutation ; Polymerase Chain Reaction ; Tuberculosis

BACKGROUND: The genetic basis of isoniazid (INH) resistance in Mycobacterium tuberculosis has been attributed to at least four genes. Mutations of the katG gene encoding catalase-eroxidase have been shown to cause resistance to INH. Among these mutations, the point mutation of codons 315 and 463 are frequently found. To determine whether simple screening method could potentially be useful for the detection of INH-esistant strains, we investigated the presence of mutation of codons 315 and 463 of katG gene in INH-esistant M. tuberculosis from Korea. METHODS: We used the polymerase chain reaction (PCR) and direct sequencing analysis to detect the point mutation of codons 315 and 463 of katG gene in 48 strains of INH-esistant and 10 strains of INH-usceptible M. tuberculosis. RESULTS: No amplification product was generated from 2 of 48 INH-esistant strains. Among the remaining 46 isolates, point mutations at codons 315 and 463 were identified in 19 isolates (41.3%) and 40 isolates (87.0%), respectively. Among the 10 INH-usceptible strains, the codon 463 point mutation was identified in 7 isolates, however, no point mutation of the codon 315 was found. CONCLUSIONS: These results suggest that the point mutation of codon 463 of katG gene of M. tuberculosis may be a polymorphism not related with INH resistance. Although the mutation of codon 315 of katG gene was limited to 41.3% of INH-esistant isolates, further investigation of the codon 315 of katG gene should lead to increased understanding of resistance genes and the deveolpment of rapid molecular detection methods.
Codon ; Isoniazid ; Korea* ; Mass Screening ; Mycobacterium tuberculosis* ; Mycobacterium* ; Point Mutation ; Polymerase Chain Reaction ; Tuberculosis