3.Effects of extracellular-signal regulated kinase signaling pathway activation on hypoxic-ischemic brain damage of neonatal rats
Chunming JIANG ; Yan MI ; Zhuying WANG
Chinese Journal of Perinatal Medicine 2011;14(2):121-125
Objective To investigate the effects of extracellular-signal regulated kinase (ERK) on hypoxic-ischemic brain damage of neonatal rats.Methods The hypoxic-ischemic brain damage model of neonatal Wistar rats was established as following:first the right common carotid artery of the rats was ligated;2 h after operation,the rats began to inhale 8%-oxygen oxygen-nitrogen gas mixture lasting for 2 h.Rats were randomly divided into three groups:sham-group,hypoxic-ischemic group and ERK inhibitor PD98059 group (the rats were injected PD98059 2 mg/kg 10 min before the ligation).Six hours after the models were done,hippocampi and cortex of the ligation side of rats in the three groups were collected,and the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured.Apoptosis of neuron was assessed by TUNEL staining.The expression of ERK1,ERK 2,Bcl-2 and Bax were examined by Western blot.The differences among the groups were analyzed with ANOVA and q test.Results Compared with the sham-group,the MDA level [(342.9± 10.8) μmol/L vs (181.5± 17.0) μmol/L,q= 6.35,P<0.01) and the apoptosis rate of neuron [(18.80±1.37)% vs (3.53±0.34)%,q=6.06,P<0.01) of hypoxic-ischemic group was higher,and SOD level was lower [(34.8±4.3) U/ml vs (63.4±4.3) U/ml,q=4.99,P<0.01].While the apoptosis rate of neuron [(15.53±0.64) %] and MDA level [(252.0± 17.1) μmol/L] of PD98059 group were lower than those of hypoxic-ischemic group(q=3.87 and 5.28,P<0.01respectively),the SOD level [(51.5 ± 3.8) U/ml] was higher than that of hypoxic-ischemic group (q=4.17,P<0.01).There were no differences of ERK1 and ERK2 expressions among the three groups.The phosphorylated ERK1 and ERK2 levels of hypoxic-ischemic group were higher than those of sham-group (q=3.82 and 4.08,P<0.01) and PD98059 group (q=4.79 and 5.12,P<0.01).The expression of Bcl-2 and Bax of hypoxic-ischemic group were higher than those of sham-group (q=3.55 and 3.42,P<0.01).Compared with hypoxic-ischemic group,Bcl-2 expression (q=3.71,P<0.01) of PD98059 group was higher,and Bax expression (q=5.86,P < 0.01) was lower.Conclusions ERK is involved in hypoxic-ischemic brain damage of neonatal rats through regulating the expression of apoptosis protein.
4.Study on the shearing bond strength between Vita Mark II and two kinds of veneering ceramics
Yanqing HONG ; Yong MI ; Tao JIANG
Journal of Practical Stomatology 2014;(2):169-172
Objective:To compare the shear bonding strength between two kinds of veneering ceramics respectively bonded to Vita Mark II.Methods:Vita Mark II samples were prepaired in the size of 8 mm ×6 mm ×2 mm.Veneering ceramic VM9 and Titankeram-ik were sintered on the Vita Mark II blocks respectively(n =1 0).The shear bond strength of each specimen was measured,the failure mode of the fracture surface was observed by SEM.The element in the bonding interface was analysed by field emission scanning elec-tron microscope(FE-SEM).Results:The values of shear bond strength (MPa)of VM9 and Titankeramik groups were 36.1 7 ±8.91 and 42.07 ±8.31 respectively(P >0.05).SEM observation showed tight bond between the two materials,FE-SEM results showed penetration of Al element from MarkⅡblock into veneering porcelain VM9 or Titankeramik.Conclusion:Veneered porcelain of VM9 and Titankeramik can be used as the individual veneering ceramics for Vita Mark II blocs.
5.The study for recombinant E.coli LLO/OVA regulating the function of CD4~+ CD2~+ Treg cells in mice
Man XU ; Xiaowei JIANG ; Can MI
Chinese Journal of Immunology 1985;0(05):-
Objective:To investigate the role of recombinant E.coli LLO/OVA on regulating the function of murine CD4+ CD25+ Treg cells.Methods:After E.coli LLO/OVA or E.coli OVA vaccination,the murine spleen CD4+ CD25+ Treg,CD4+ CD25- T and CD11c cells were collected respectively by magnetic beads sorting.The concentration of IL-10 in the supernatant of mix cocultured CD4+ CD25+ Treg and CD11c cells,and the suppression role of CD4+ CD25+ Treg cells on the proliferation of CD4+ CD25- T cells were determined.The percentage of OVA specific CD8+ T cells in mouse spleen was analyzed by flow cytometry.The number of metastatic tumor nodules in lungs of the mice transplanted with B16-OVA subcutaneouly was compared before and after dilition of CD4+ CD25+ Treg cells in mice.Results:Compared to E.coli OVA,E.coli LLO/OVA significantly downregulated IL-10 secretion of CD4+CD25+Treg cells and attenuated the suppressive effect of CD4+ CD25+ Treg on the proliferation of CD4+ CD25- T cells(P
6.Determination of [6]-Gingerol in Rat Plasma by HPLC-UV
Suzhen JIANG ; Suiqing MI ; Ningsheng WANG
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To develop a HPLC-UV method for the determination of [6]-gingerol in the rat plasma.Methods [6]-Gingerol was extracted from the rat plasma by using liquid-liquid extraction,then was separated on a Hypersil C18 column(250 mm?4.6 mm,5 ?m).The mobile phase was acetonitrile and water(45:55,v/v) with a flow-rate of 1.0 mL/min.The eluate from the HPLC column was monitored by the spectrophotometric detector at 280 nm.The injection volume was 20 ?L.[6]-Gingerol was identified based on its retention time compared with the reference standard.Results The linear calibration curve was obtained in the concentration range of 0.25~5.0 ?g/mL.The low quantitative limit was 0.1 ?g /mL.The RSD of inter-day was
9.Analysis of gastric bilirubin absorbance values and gastric pH monitoring in children with primary duodenogastric reflux.
Mi-Zu JIANG ; Xiao-Lei HUANG ; Jin-Dan YU
Chinese Journal of Pediatrics 2007;45(4):301-303
Adolescent
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Bilirubin
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metabolism
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Child
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Child, Preschool
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Duodenogastric Reflux
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metabolism
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Esophageal pH Monitoring
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Female
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Humans
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Male
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Stomach
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physiopathology
10.Phylogenetic Analysis of Acinetobacter baumannii Strains in Elderly
Jianming ZHU ; Rujin JIANG ; Kangle WU ; Hui JIN ; Zuhuang MI
Chinese Journal of Nosocomiology 2006;0(12):-
OBJECTIVE To investigate the phylogenetic analysis of Acinetobacter baumannii strains in elderly.METHODS The 33 kinds resistant genes were detected by PCR and cluster analysis.RESULTS The positive rates of AmpC(chromosome type),TEM and PER were 85%,55% and 25%,respectively.The positive rates of aac(3)-Ⅰ,ant(3″)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb and armA were 95%,95%,40%,15% and 35%,respectively.The positive rate of qacE△1-sul1 was 75%.There were very high positive percentages of AmpC(chromosome type),TEM,PER,aac(3)-Ⅰ,ant(3″)-Ⅰ,armA and qacE△1-sul1 genes in A.baumannii isolates among elderly.There were clones transmitted phenomenon.CONCLUSIONS The resistance phenotype of A.baumannii isolates among elderly is in accordance with genetic testing.A.baumannii can induce clones transmitted hospital infection.