This paper analyze the dynamic observation results ofCD4+ and CD8+ T lymph cell of13 cases HIV/AIDS patients, who have survived for more than ten years.The analysis show that long term Chinese medicine intervention have effect in increasing and maintaining CD4+ T lymph cell, and activating or adjusting CD8+ lymph cell.Chinese medicine also has the effect in delaying the progression ofAIDS and prolonging longevity.

Objective:To describe the current situation of IHR ( 2005 ) public health emergency core capacity at the provincial, city, district or county level for health departments in China, discover gaps in the implementation of IHR(2005) public health emergency core capacity, and thereby provide reference for the construction of IHR(2005) public health emergency core capacity. Methods: A survey was conducted from 211 questionnaires of IHR (2005) public health emergency core capacity, which included 7 provinces, 64 cities, and 140 counties ( districts) in the health departments. Results:The indicator of IHR (2005) public health emergency core capacity is suitable for the conditions in China;there is a lack of institution building in China concerning IHR (2005) public health emergency core capacity;there is a decreasing trend at the provincial, city and county ( district) level. Conclusion:Recommen-dations were proposed to strengthen China’s institution building of IHR (2005) public health emergency core capaci-ty, focus on strengthening construction of county (district) level IHR (2005) public health emergency core capacity, strengthen the weak links of the IHR (2005) public health emergency core capacity index, strengthen China’s appli-cability research concerning IHR (2005) public health emergency core capacity.

Objective To investigate the possible correlation between chronic periodontitis(CP)and chronic renal failure(CRF)by establishing chronic periodontitis and chronic renal failure model in SD rats. Methods Forty health male SD rats were divided into four groups: control group(A), CP group(B), CRF group(C), CP accompany with CRF group(D). Ten rats were sacrificed in every group at the end of week 8. The periodontal index, levels of serum Scr and BUN, the concentration of IL-1β and TNF-α were examined. The severity CP and CRF was quantified by histopathology. The date was statistically analyzed. Results Animal models were established successfully. Scr and BUN in group D, BUN were higher than that in group C[Scr(120.54±21.29)junol/L vs(93.63±18.82)u,mol/L, BUN(34.20±14.44)mmol/L vs(17.77±4.15)mmol/L, P<0.05]. The kidney change of inflammation was observed in group B, the grade of PAS and Masson in group C and D were higher than that in group A(P<0.01), and that in group D was higher than group C(P<0.05). Obvious inflammation of periodontal tissue was observed in group B and D. Attachment loss level(AL)in group D was higher than that in group B[(173.60± 16.75)μm vs(124.00±23.87)μm, P<0.05]. The level of IL-lβ and TNF-α in group B and C and D were higher than that in group A(P<0.05), and IL-lβ in group D was higher than that in group B and C(P<0.05), TNF-a in group D was higher than that in group B(P<0.05). 2×2 factorial design revealed that there were interactions between CP and CRF on the numerus of Scr and BUN and AL P<0.05), and the influence of each factor on that was significant(P<0.05), no interactions were noted between CP and CRF on IL-1β and TNF-α(P>0.05), but the influence of each factor on that was significant(P<0.05). Conclusions The SD rat models can appear chronic periodontitis and chronic renal failure at the same time. There is correlation between chronic periodontitis and chronic renal failure. Chronic periodontitis can aggravate chronic renal failure throngh the role of inflammation.

Objective To compare the esophageal acid and alkaline exposure characteristics of patients with cardia carcinoma after proximal gastrectomy or total gastrectomy.Method A total of 77 patients of cardia carcinoma who underwent radical resection from Sep 2007 to Sep 2011 in our hospital were retrospectively reviewed.24 hour esophageal pH monitoring were performed in all patients.Result Patients were divided into three groups:group TG had total gastrectomy (n =25),group PP had proximal gastrectomy with pyloroplasty (n =33),group NP had proximal gastrectomy (n =19).It revealed that indicators of acid reflux including the overall time length of acid episodes,＞ 5 min times of acid episodes,duration of longest acid episodes,time length of pH ＜ 4.00 and the DeMeester Scores in group NP were significantly higher than in group PP(U =32,P ＜ 0.01 ; U =35,P ＜ 0.01 ; U =23,P ＜ 0.01 ; U =39,P ＜0.01 ;U =49,P ＜0.01 respectively).Only alkline reflux was observed in group TG.The total times of alkaline episodes in PP group was significantly lower than in group TG(U =52,P ＜0.01) and group NP (U =182,P ＜0.01).＞5 min times of alkaline episodes in group TG was larger than in group PP,and that in group PP was larger than in group NP(P ＜0.01).Duration of longest alkaline episodes and total period of pH ＞7.00 in group PP was significantly higher than in group TG(U =125,P ＜ 0.01 ; U =143.5,P ＜ 0.01),and that in group TG was higher than in group NP(U =23.5,P ＜ 0.01 ; U =14,P ＜ 0.01).Conclusions Alkaline reflux deserves more attention in evaluating esophageal reflux in patients with cardia carcinoma after resection.Pyloroplasty is not helpful to relieving esophageal acid episodes while causing severe alkaline reflux.

Objective To investigate the effect of PKG on the secretion of IL-6, IL-10, and TNF-αin THP-1 macrophage-derived foam cells. Methods THP-1 monocytes were induced to construct macrophages by treating with 160 nmol/L TPA. Then the macrophages were further treated with 50 mg/L ox-LDL to become foam cells. Four groups were set in this study, including the macrophage group, the foam cell group, the group of foam cell treated with PKG agonist 8-Br-cGMP, and the group of foam cell treated with PKG inhibitor KT-5823. The morphology of THP-1 cells, macrophages and foam cells were observed under microscope. The cellular lipid accumulation was detected by oil red ostaining. The secretion of IL-6, IL-10, and TNF-α into the supernatant was detected by ELISA assay. Results The foam cell was obtained after macrophage incubated with ox-LDL for 48 hours. The secretion of IL-6 and TNF-α increased significantly from the foam cells than that from the macrophages (P<0.05). After the THP-1 monocyte-derived macrophages were incubated with 8-Br-cGMP, the secretion of IL-6 in the supernatant decreased significantly ( P < 0 . 05 ) and IL-10 level in the supernatant increased significantly (P < 0.05). After the macrophages were incubated with KT-5823, the secretion of IL-10 decreased significantly (P<0.05), but the secretion of IL-6 was not significantly changed (P>0.05). After incubation with 8-Br-cGMP, the secretion of IL-6 and TNF-α from the macrophage-derived foam cells decreased significantly (P < 0.05), but IL-10 increased significantly (P<0.05). After the foam cells were treated with KT-5823, the secretion of IL-6 and TNF-αwere also decreased significantly (P<0.05), with no significant change of IL-10 secretion (P > 0.05). Conclusions PKG may enhance the expression of anti-inflammatory cytokine IL-10, and inhibit the expression of inflammatory cytokine IL-6 and TNF-α, contributing to prevent the development of inflammation. PKG might have a potential anti-atherosclerosis effect.

ObjectiveTo investigate the association of bacterial translocation (BT) with cachexia and its impact on the outcome of gastric cancer patients.MethodsSixty cachectic gastric cancer patients,50 age- and sex-matched non-cachectic gastric cancer patients,and 55 healthy controls were enrolled in this study between January 2008 and July 2009.Polymerase chain reaction was performed to detect bacterial DNA in the peripheral blood of cancer patients and healthy controls,Cytokine levels were tested by enzyme-linked immunosorbent assay.Flow cytometry was used to detect immune indicators.All the enrolled patients were followed up for two years,and the two-year survival rate was calculated.ResultsThe BT ratio was significantly higher in cachectic patients than in non-cachectic patients (25.0％ vs.8.0％,P =0.019) and healthy controls (25.0％ vs.0.0％,P =0.000).BT-positive cachectic patients had significantly higher levels of IL-1α,IL-6,TNF-α,and IFN-γ compared with BT-negative cachectic patients ( P =0.012,0.003,0.036,and 0.017,respectively ) and BT-positive non-cachectic patients ( P =0.011,0.034,0.000,and 0.022,respectively).The two-year survival rate in BT-positive cachectic patients was significantly lower than in BT-negative cachectic patients (P =0.023 ).The levels of CD3 +T,CD4+ T,natural killer cells,and CD4 + T/CD8 + T in gastric cancer patients were significantly lower than in healthy controls ( P =0.023,0.031,0.016,0.041,respectively),whereas CD8 + T level was significantly higher in gastric cancer patients than in healthy controls (P =0.038).ConclusionBT may contribute to the development of cancer cachexia and influence the long-term survival of locally advanced gastric cancer patients.

OBJECTIVE To investigate the disinfectant and antibiotic resistance genotypes in 60 strains of Escherichia coli isolated from urine.METHODS Sixty strains of E.coli isolated from inpatients′ urine were collected from Jan 2006 to Oct 2008.Antibiotic susceptibility tests for fifteen antibiotics were performed by Kirby-Bauer method.And three kinds of disinfectant and antibiotic resistance genes(qacE△1,tehA,merA)were analyzed by polymerase chain reaction(PCR) and DNA sequencing.RESULTS More than 70.0% of the sixty strains of E.coli were susceptible to imipenem,piperacillin/tazobactam,cefoxitin,amikacin and gentamicin,and less than 50.0% were susceptible to the other ten antibiotics.There were 42 strains with qacE△1 gene(70.0%),10 strains with merA gene(16.7%) and all strains with tehA gene.The sequence of the first strain was different from those reported in GenBank,so it was a new subtype.CONCLUSIONS There are 70% of E.coli strains isolated from urine samples with qacE△1 gene.And disinfectant resistance may be one of the main factors for hospital infection in the future.

Objective: To investigate the effects of simulated altitude acute hypoxia on appetite and orexin mRNA expression in rats. Methods: Rats were exposed to hypobaric chambersimulating different high altitude (3000m,4000m,5000m,6000m) for different times (8h/d;1d,3d and 7d respectively), and the changes of appetite, weight gain, and the orexin mRNA expression in hypothalamus of rats were examined. Results: With the increasement of hypoxia time and simulated altitude, the total food intake of rats and the orexin A and B mRNA expression in hypothalamus decreased gradually as compared with control. After 7 days of hypoxia, the weight gain of rats was significantly different(P

Acquired Immunodeficiency Syndrome ; drug therapy ; epidemiology ; Antiretroviral Therapy, Highly Active ; adverse effects ; methods ; China ; epidemiology ; Female ; HIV Infections ; drug therapy ; epidemiology ; Humans ; Male ; Minority Groups

Objective To retrospectively evalute the value and accuracy of adenosine stress and rest SPECT myocardial perfusion imaging. Methods A total of 1858 patients who were suspected or known for coronary artery disease (CAD) underwent 99Tcm-methoxyisobutylisonitrile ( MIBI ) myocardial perfusionSPECT with adenosine infusion using the standard 2-day protocol. Images were interpreted by two or more experienced nuclear medicine physicians . Coronary angiography was carried out in all patients within one month. Kappa test was used to analyze the correlation between the two imaging studies. Results By coronary angiography, there were 957 patients diagnosed of CAD (one-, two-, three-vessel disease: 506,256,195, respectively) and 901 normal. Stenosis was found in 1603 vessels, including left anterior descending coronary artery (LAD): 765, left circumflex coronary artery (LCX): 399 and right coronary artery (RCA): 439. By adenosine induced stress myocardial perfusion imaging, 876 patients were diagnosed of myocardial ischemia ( sensitivity: 876/957, 91.54% ) and 651 patients had negative findings ( specificity:651/901,72.25 % ). The positive and negative predictive values were 77.80% ( 876/1126 ) and 88.93% (651/732), respectively. The correlation coefficient between the two imaging studies was 0.641. The vessel-based sensitivity was 81.31% (622/765) for LAD, 56.64% (226/399) for LCX and 70.62% (310/439) for RCA, respectively. The sensitivity for detection of one-, two-, three-vessel stenosis was 87.55% (443/506), 94.92% (243/256) and 97.44% (190/195), respectively. The side-effects was mild and transient with an incidence rate of 84.12% ( 1563/1858), without major cardiac events. Conclusion Stress myocardial perfusion imaging induced by adenosine is reliable for the evaluation of myocardial blood supply in CAD patients.