1.Medication and Flying: A Pilot's guide.
Han Yong LEE ; Mi Hye LIM ; Yong Ho LEE
Korean Journal of Aerospace and Environmental Medicine 1998;8(4):369-378
No abstract available.
Diptera*
2.Establishment of Embryonic Stem Cell Line from Isolated Blastomeres from Mouse Preimplantation Embryos.
Chun Kyu LIM ; Ji Hye SUNG ; Hye Won CHOI ; Jae Won CHO ; Mi Ra SHIN ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2006;33(1):25-34
OBJECTIVE: The aim of this study was to investigate whether embryonic stem (ES) cells can be established from isolated blastomeres of mouse embryos. METHODS: Blastomeres were separated from mouse (C57Bl/6J) 2- or 4-cell embryos. Isolated blastomeres or whole 4-cell embryos were co-cultured with mitosis-arrested STO feeder cells in DMEM supplemented with recombinant murine leukemia inhibitory factor and ES-qualified fetal bovine serum. After the tentative ES cell lines were maintained from isolated blastomeres or whole embryos, some of them were frozen and the others were sub-cultured continually. Characteristics of tentative ES cell lines as were evaluated for specific gene expressions with immunocytochemistry and RT-PCR. RESULTS: One ES cell line (3.0%) was established from isolated blastomere of 2-cell embryo and one cell line (4.0%) from isolated two blastomeres of 4-cell embryo. And five cell lines (16.7%) were established from whole 4-cell embryos. Both cell lines from isolated blastomere and whole embryo expressed mouse ES cells specific markers such as SSEA-1, Oct-4 and alkaline phosphatase. Marker genes of three germ layers were expressed from embryoid bodies of both cell lines. CONCLUSION: This study suggests that mouse ES cells could be established from isolated blastomeres, although the efficiency is lower than whole embryos. This animal model could be applied to establishment of autologous human ES cells from biopsied blastomeres of preimplantation embryos in human IVF-ET program.
Alkaline Phosphatase
;
Animals
;
Antigens, CD15
;
Blastocyst*
;
Blastomeres*
;
Cell Line
;
Embryoid Bodies
;
Embryonic Stem Cells*
;
Embryonic Structures
;
Feeder Cells
;
Gene Expression
;
Germ Layers
;
Humans
;
Immunohistochemistry
;
Leukemia Inhibitory Factor
;
Mice*
;
Models, Animal
3.A Korean Version of Advanced Cardiac Life Support (ACLS) in Perspective.
Hee Jin AHN ; Mi Hye LIM ; Bock Soon HAN
Korean Journal of Aerospace and Environmental Medicine 1998;8(3):255-261
No abstract available.
Advanced Cardiac Life Support*
4.Torsion and ruptured dysgerminoma of ovary in pregnancy.
Chang Kyo LIM ; Hye Young KIM ; Jang Yeoun KWON ; Dae Hyun KIM ; Mi Yeoun CHO
Korean Journal of Obstetrics and Gynecology 1992;35(2):292-296
No abstract available.
Dysgerminoma*
;
Female
;
Ovary*
;
Pregnancy*
5.A comparative study on family function between the psychiatric families and the normal families.
Kyu Rae LEE ; Jong Han LIM ; Mi Kyung OH ; Hye Ree LEE ; Bang Bu YOUN
Journal of the Korean Academy of Family Medicine 1991;12(1):30-35
No abstract available.
Humans
6.Congenital Anonychia of the Toes with Absence of Underlying Phalangeal Bones.
Seong Jin KIM ; Mi Hye LIM ; Seung Chul LEE ; Young Ho WON ; Inn Ki CHUN
Annals of Dermatology 1997;9(3):188-190
Congenital absence of nails usually occur as a rare isolated anomaly or combined with other ectodermal defects. This anomaly is regarded as an inherited disorder either dominantly or recessively but quite a few cases were reported as sporadically developed. The patient was a 2-month-old girl who had no nails on both her 2nd, 3rd toes but had rudimentary nails on her left big toe and both 4th toes at birth. We could not find any other congenital deformity, any family history of inherited diseases related to anonychia. Radiological findings revealed no visualization of both 4th distal phalanges, only. We report this case as congenital anonychia of a sporadic type which may have developed independently from an underlying bone abnormality. We also review other reported cases in the literature.
Congenital Abnormalities
;
Ectoderm
;
Female
;
Hallux
;
Humans
;
Infant
;
Parturition
;
Toes*
7.A Case of Disseminated Pagetoid Reticulodsis.
Hyang Mi KWON ; Sun Seong PARK ; Hye Lim JUNG ; Dong Hyuck KEUM ; Kye Yong SONG
Journal of the Korean Pediatric Society 1995;38(7):988-992
No abstract available.
8.Protocol for Physical Restraints of Patients in Nursing Homes.
Journal of Korean Academy of Fundamental Nursing 2013;20(4):345-358
PURPOSE: The purpose of this study was to develop a protocol that would help prevent accidents, apply physical restraints properly, and reduce the use of physical restraints in nursing homes. METHOD: A review of the literature and analysis of existing statutes and regulations were used to develop the preliminary protocol. To test the validity of this preliminary protocol, ten experts were selected from academia and clinical practice to review the protocol. The initial protocol was finalized after it had been reviewed by experts and tested for clinical validity in five different nursing homes. RESULT: The protocol consists of objectives, definitions and accident probability assessment, principle of using physical restraints, monitoring and documentation of physical restraints. CONCLUSION: The findings of this study can be used as guidelines to focus on preventing accidents arising out of use of physical restraints, assessing the probability of accidents, and reducing the use of physical restraints through preventive interventions. This will be helpful to prevent ethical, physical, or psychological problems arising from use of physical restraints and to protect the rights of elderly people in nursing homes.
Aged
;
Human Rights
;
Humans
;
Nursing Homes*
;
Nursing*
;
Restraint, Physical*
;
Social Control, Formal
9.Risk Factors Contributing to Extrauterine Growth Restriction in Very Low Birth Weight Infants
Neonatal Medicine 2024;31(2):31-37
Purpose:
Despite advances in neonatal care, extrauterine growth restriction (EUGR) remains common in preterm infants. This retrospective single-center study aimed to determine the incidence and risk factors of EUGR in very low birth weight (VLBW) infants.
Methods:
Data were collected concerning VLBW infants with gestational age (GA) <32 weeks between 2011 and 2020. EUGR was defined as a decline in weight z-score >1.2 from birth to discharge, using Fenton growth charts.
Results:
Among 331 eligible preterm infants, the prevalence of EUGR at discharge was 71.6%. Infants with EUGR had lower GA and birth weight than those without EUGR. They also underwent prolonged durations of parenteral nutrition, invasive ventilation, and hospitalization. Neonatal morbidities, such as bronchopulmonary dysplasia, patent ductus arteriosus, necrotizing enterocolitis, gastrointestinal surgery, sepsis, and parenteral nutrition-associated cholestasis were more prevalent in the EUGR group. Multivariate analysis identified lower GA and longer time to reach full enteral feeding as independent risk factors, whereas maternal use of antenatal steroids and history of gestational diabetes mellitus were independent protective factors for EUGR.
Conclusion
As VLBW infants are at a high risk of EUGR, continuous attention and efforts to achieve early full enteral nutrition are required to decrease the incidence of EUGR.
10.Reliability of the Single Cell PCR analysis for Preimplantation Genetic Diagnosis of Single Gene Disorders.
Hye Won CHOI ; Hyoung Song LEE ; Chun Kyu LIM ; Mi Kyoung KOONG ; Inn Soo KANG ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2005;32(4):293-300
No abstract available.
Epidermolysis Bullosa
;
Muscular Dystrophy, Duchenne
;
Ornithine Carbamoyltransferase
;
Polymerase Chain Reaction*
;
Preimplantation Diagnosis*