Objective To study apoptosis and morphological characteristics in the developing renal corpuscles of mouse kidney. Methods Light,transmission electron microscope and TUNEL technique were used to observe apoptosis in the developing renal corpuscles of different embryonic and postnatal mice. Results Apoptotic cells could be found when renal corpuscles occurred at embryonic day 14(E14).It peaked around E18 and decreased thereafter.Electron microscope revealed that apoptotic cells had morphological characteristics such as margination of condensed nuclear chromatin,shrinking cytoplasm.Apoptosis of endothelial cells and podocytes were more frequent.Two results of apoptotic cells were observed,1. being ingested by neighboring cells; 2. apoptotic cells were dropping into glomerular capillary lumens or Bowman capsules.Conclusion Apoptotic cells were found in all the time during the development of mouse renal corpuscles.It might play an important role in the development of renal corpuscles.

Objective To investigate the clinical significance of the rate of spontaneous apoptosis and the expression of p27kip1 in transitional cell carcinoma of bladder (BTCC). Methods Immunohistochemical analysis of p27kip1 was performed on paraffin embedded tissue sections in 50 cases of BTCC, by immunohistochemical method S-P. The terminal deoxynucleotidyl transferase- mediated dUTP- biotin nick end labeling (TUNEL) was used to examine the level of apoptotic cells in 50 cases of BTCC. Results In BTCC, the spontaneous apoptosis index AI was (3.0?1.5)%, the positive rate of p27kip1 was 58.0 %(29/50). Both of them decreased with the escalation of the clinicopathologic grade and stage. The positive expression of p27kip1 protein were significantly associated with higher spontaneous apoptosis (P

Objective To study the correlation between the serum high mobility group protein B1 (HMGB1)and the acute organophosphorus pesticide poisoning(AOPP). Methods The serum HMGB1 levels of the 116 patients with AOPP(AOPP group)and 40 healthy adults(control group)were detected by immunoblotting method. According to illness severity, AOPP group was divided into mild group(40 cases),moderate group(39 cases)and severe group(37 cases), and severe group was divided into multiorgan dysfunction syndrome(MODS)group(20 cases)and no MODS group(17 cases). The serum levels of CHE,HMGB1 were compared. Results The absorbance of HMGB1 in severe group(2.91±0.12)was significantly higher than that in moderate group(2.15±0.17), mild group(1.16 ± 0.29)and control group (0.84±0.30)(P＜0.01).The absorbance of HMGB1 in moderate group was significantly higher than that in mild group and control group(P＜0.01). The absorbance of HMGB1 in mild group was significantly higher than that in control group(P＜0.05). The absorbance of HMGB1 in MODS group was significantly higher than that in no MODS group(P＜0.01),but the absorbance of CHE had no significant difference between two groups(P＞0.05). Conclusions The degree of AOPP has notable correlation with the level of serum HMGB1. The level of serum HMGB1 is an useful index for evaluating the degree of AOPP.

Objective To study the effects of midazolam on expression of vascular endothelial growth factor (VEGF) of gerbils following total cerebral ischemia-reperfusion injury to look for an experimental basis for the rational clinical use of midazolam. Methods Seventy-two male gerbils (Mongolian gerbil) were randomly assigned into three groups (24 each): sham injury group, injury group and midazolam treatment group. Total cerebral ischemia was reproduced by blocking the bilateral carotid arteries for 10 minutes with bulldog clamps. When reperfusion began, with release of the clamps, 5mg/kg of midazolam was intraperitoneally injected to the animals in midazolam group, and 50ml/kg of normal saline was given by the same way in the gerbils in injury group. Then the parameters listed below were observed: positron emission tomography (PET) images at 6h, 1d, 3d and 7d after reperfusion, and the expression of VEGF in cerebral tissue was immunohistochemically assessed. Results No obvious abnormality was found in the cerebral tissue of sham injury group. For the animals in the injury group and midazolam treatment group, the brain reinfusion area enlarged obviously (P

Objective To investigate the effect of the mascarinic acetylcholine receptor (mAChR) agonist Oxo-tremorine-M (Oxo-M) on glycinergic spontaneous inhibitory postsynaptic currents (sIPSCs) and micro-inhibitory postaynaptic currents (mIPSCs) in lamina Ⅱ neurons in the spinal cord of rats. Methods Glycinergic IPSCs (sIPSCs and mIPSCs) in lamina Ⅱ neurons of spinal slices were recorded using the whole-cell voltage-clamp method. The non-selective mAchR ngonist Oxo-M was applied through bath perfusian. The effects of Oxo-M 1, 3, 5 and 10 μmol/L on sIPSCs and mIPSCs were examined. Results Oxo-M at the concentrations of 3-10 μmol/L significantly increased the frequency of sIPSCs without changing the amplitude in 16 lamina Ⅱ neurons tested. Interestingly, when the concentration of Oxo-M was increased to 10 μmol/L, the potentiating effect of Oxo-M on the frequency of slPSCs was decreased as compared with 3 μmol/L Oxo-M in the above 16 neurons. The slPSCs were completely abolished by 2 μmol/L strychnine. Atropine, the specific mAChR antagonist, completely blocked the effect of Oxo-M on the frequency of sIPSCs. In 9 additional lamina Ⅱ neurons, 1-10 μmol/L oxo-M failed to alter significantly the frequency and amplitude of glycinergic mIPSCs. Conclusion Activation of mAChRs in the somatodendritic site of glycinergic interneurous increases the synaptic glycine input to spinal dorsal horn neurons, but not in a dose-dependent manner.

Quality control of medical equipment is very important to the diagnostic accuracy and the cure effect. The work of the quality control is the main technical basis of the hospital construction in promoting medical development, and it's also an important content of the modem medical management. The status, causes and countermeasures of medical equipment quality control in military hospital at the present time are analyzed and discussed.

0.05).After that,both systolic and diastolic function began to decrease.In 4 weeks after the operation,both the the maximum rate of left ventricular isovolumic systolic pressure + dp/dtmax and the maximum rate of left ventricular isovolumic diastolic pressure-dp/dtmax decreased to the lowest levels [(1249.89 ? 95.82) mm Hg/s and(-1316.40 ? 58.31) mm Hg/s,respectively];and then in 6 weeks after the operation,echocardiography showed that the left ventricular short axis fractional shortening FS reached the lowest level [(18.70 ? 3.83)%].Moreover,we found that the FS was highly related with the + dp/dtmax(r=0.864,P

By introducing the basic concept and principle, the advantages and characteristics of ASV technology are highlighted. Based on clinical application, suggestions on how to correctly set ASV parameters are given.

A study on abdominal skin flaps of the fresh cadavers of young men under the operation, light and scanning electron microscope, and MAS image analysis system has been made. Methods include Chinese ink, ABS or methyl methacrylate injection and histological slides, transparent specimens as well as casts preparation. The course of the vessels in the human abdominal skin flap is in the "three steps form". Trunks and main branches of the cutaneous vessels run in the deep part of the superficial fascia, their branches and terminals form anastomoses in the middle of the reticular layer of dermis, and branches from the anastomoses form microvascular network in the subpapillary layer. The vascular networks in the skin flap are concentrated in five layers: the deep fascial, superficial fascial, profund dermal, subpapillary, and papillary layers. Between the subpapillary and the profund dermal vascular networks, there is a layer devoid of vascular network and crossed by vascular arteries only. Therefore, this layer may be called as "vascular network devoid area". The differences of the area fraction (Aa) or number of vessels are not significant among various areas of the abdominal skin flap. Design in detaching the abdominal skin flaps and skin grafts is discussed.

Objective: To determine the suitable condition for enrichment and purification of total flavonoids from Scutel-laria baicalensis with macroporous resins and to select the optimal parameters. Methods: Using the total flavonoids as the standard, the optimum macroporous resins were selected. Sample concentration, adsorption duration and alcohol concentration were investigated by orthogonal experiment design of form L9(34). Results: The 1300 resin was the suitable one for enriching and purifying total flavonoids. The optimum conditions Were as follows :adsorption time at 40 min, sample concentration at 4. 00 mg/ml (pH 5. 0) ,and 30% alcohol as the elution reagent. Conclusion: 1300 resin can be used to enrich and purify the total flavonoids of Scutellaria baicalensis, which can increase the rate of enrichment.