Objective To study the correlation between the serum high mobility group protein B1 (HMGB1)and the acute organophosphorus pesticide poisoning(AOPP). Methods The serum HMGB1 levels of the 116 patients with AOPP(AOPP group)and 40 healthy adults(control group)were detected by immunoblotting method. According to illness severity, AOPP group was divided into mild group(40 cases),moderate group(39 cases)and severe group(37 cases), and severe group was divided into multiorgan dysfunction syndrome(MODS)group(20 cases)and no MODS group(17 cases). The serum levels of CHE,HMGB1 were compared. Results The absorbance of HMGB1 in severe group(2.91±0.12)was significantly higher than that in moderate group(2.15±0.17), mild group(1.16 ± 0.29)and control group (0.84±0.30)(P＜0.01).The absorbance of HMGB1 in moderate group was significantly higher than that in mild group and control group(P＜0.01). The absorbance of HMGB1 in mild group was significantly higher than that in control group(P＜0.05). The absorbance of HMGB1 in MODS group was significantly higher than that in no MODS group(P＜0.01),but the absorbance of CHE had no significant difference between two groups(P＞0.05). Conclusions The degree of AOPP has notable correlation with the level of serum HMGB1. The level of serum HMGB1 is an useful index for evaluating the degree of AOPP.

Objective To study apoptosis and morphological characteristics in the developing renal corpuscles of mouse kidney. Methods Light,transmission electron microscope and TUNEL technique were used to observe apoptosis in the developing renal corpuscles of different embryonic and postnatal mice. Results Apoptotic cells could be found when renal corpuscles occurred at embryonic day 14(E14).It peaked around E18 and decreased thereafter.Electron microscope revealed that apoptotic cells had morphological characteristics such as margination of condensed nuclear chromatin,shrinking cytoplasm.Apoptosis of endothelial cells and podocytes were more frequent.Two results of apoptotic cells were observed,1. being ingested by neighboring cells; 2. apoptotic cells were dropping into glomerular capillary lumens or Bowman capsules.Conclusion Apoptotic cells were found in all the time during the development of mouse renal corpuscles.It might play an important role in the development of renal corpuscles.

Objective To investigate the clinical significance of the rate of spontaneous apoptosis and the expression of p27kip1 in transitional cell carcinoma of bladder (BTCC). Methods Immunohistochemical analysis of p27kip1 was performed on paraffin embedded tissue sections in 50 cases of BTCC, by immunohistochemical method S-P. The terminal deoxynucleotidyl transferase- mediated dUTP- biotin nick end labeling (TUNEL) was used to examine the level of apoptotic cells in 50 cases of BTCC. Results In BTCC, the spontaneous apoptosis index AI was (3.0?1.5)%, the positive rate of p27kip1 was 58.0 %(29/50). Both of them decreased with the escalation of the clinicopathologic grade and stage. The positive expression of p27kip1 protein were significantly associated with higher spontaneous apoptosis (P

Objective To investigate the effect of the mascarinic acetylcholine receptor (mAChR) agonist Oxo-tremorine-M (Oxo-M) on glycinergic spontaneous inhibitory postsynaptic currents (sIPSCs) and micro-inhibitory postaynaptic currents (mIPSCs) in lamina Ⅱ neurons in the spinal cord of rats. Methods Glycinergic IPSCs (sIPSCs and mIPSCs) in lamina Ⅱ neurons of spinal slices were recorded using the whole-cell voltage-clamp method. The non-selective mAchR ngonist Oxo-M was applied through bath perfusian. The effects of Oxo-M 1, 3, 5 and 10 μmol/L on sIPSCs and mIPSCs were examined. Results Oxo-M at the concentrations of 3-10 μmol/L significantly increased the frequency of sIPSCs without changing the amplitude in 16 lamina Ⅱ neurons tested. Interestingly, when the concentration of Oxo-M was increased to 10 μmol/L, the potentiating effect of Oxo-M on the frequency of slPSCs was decreased as compared with 3 μmol/L Oxo-M in the above 16 neurons. The slPSCs were completely abolished by 2 μmol/L strychnine. Atropine, the specific mAChR antagonist, completely blocked the effect of Oxo-M on the frequency of sIPSCs. In 9 additional lamina Ⅱ neurons, 1-10 μmol/L oxo-M failed to alter significantly the frequency and amplitude of glycinergic mIPSCs. Conclusion Activation of mAChRs in the somatodendritic site of glycinergic interneurous increases the synaptic glycine input to spinal dorsal horn neurons, but not in a dose-dependent manner.

Quality control of medical equipment is very important to the diagnostic accuracy and the cure effect. The work of the quality control is the main technical basis of the hospital construction in promoting medical development, and it's also an important content of the modem medical management. The status, causes and countermeasures of medical equipment quality control in military hospital at the present time are analyzed and discussed.

0.05).After that,both systolic and diastolic function began to decrease.In 4 weeks after the operation,both the the maximum rate of left ventricular isovolumic systolic pressure + dp/dtmax and the maximum rate of left ventricular isovolumic diastolic pressure-dp/dtmax decreased to the lowest levels [(1249.89 ? 95.82) mm Hg/s and(-1316.40 ? 58.31) mm Hg/s,respectively];and then in 6 weeks after the operation,echocardiography showed that the left ventricular short axis fractional shortening FS reached the lowest level [(18.70 ? 3.83)%].Moreover,we found that the FS was highly related with the + dp/dtmax(r=0.864,P

By introducing the basic concept and principle, the advantages and characteristics of ASV technology are highlighted. Based on clinical application, suggestions on how to correctly set ASV parameters are given.

Objective To study the effects of midazolam on expression of vascular endothelial growth factor (VEGF) of gerbils following total cerebral ischemia-reperfusion injury to look for an experimental basis for the rational clinical use of midazolam. Methods Seventy-two male gerbils (Mongolian gerbil) were randomly assigned into three groups (24 each): sham injury group, injury group and midazolam treatment group. Total cerebral ischemia was reproduced by blocking the bilateral carotid arteries for 10 minutes with bulldog clamps. When reperfusion began, with release of the clamps, 5mg/kg of midazolam was intraperitoneally injected to the animals in midazolam group, and 50ml/kg of normal saline was given by the same way in the gerbils in injury group. Then the parameters listed below were observed: positron emission tomography (PET) images at 6h, 1d, 3d and 7d after reperfusion, and the expression of VEGF in cerebral tissue was immunohistochemically assessed. Results No obvious abnormality was found in the cerebral tissue of sham injury group. For the animals in the injury group and midazolam treatment group, the brain reinfusion area enlarged obviously (P

Objective To investigate the serum concentration of interleukin (IL)-17 in patients with active rheumatoid arthritis and the clinical implication. Methods Serum IL-17 level of 51 active RA patients were assessed by enzyme-linked immunosorbentassay (ELISA). The relationship between serum levels of IL-17 in active RA patients and clinic features were analyzed. Two independent samples t test, ANOVA for repeated, one-way ANOVA and Spearman's Chi-square test were used for statistical analysis. Results ① Serum IL-17 levels were elevated in active RA patients (365±63) pg/ml than in normal controls (86±13) pg/ml (P<0.01); ② Serum IL-17 levels in active RA was correlated with morning stiffiness duration, swelling index, Ritchie index, CRP, ESR, RF, X-ray score, DAS score (r =0.423, 0.213, 0.252, 0.276, 0.346, 0.251, 0.412, 0.398; P<0.05). ③ The levels of IL-17 between phase I (296±79) pg/ml and Phase II (360±89) pg/ml, phase II and phase HI (464±74) pg/ml, Phase I and Phase Ⅳ (369±83) pg/ml in RA group were statistically significantly different (P<0.05). Conclusion The levels of serum IL-17 in active RA patients are higher than the normal controls. The elevated serum IL-17 levels may be the useful markers for active RA.In addition,interleukin-17 is involved in disease progression and bony erosion of RA, and it maybe a new treatmenttarget of RA.

Objective To explore the role of IL-18 in the pathogenesis of chronic eczema. Methods Twenty-seven patients with chronic eczema were enrolled into this study along with 12 normal human controls. The severity of eczema was evaluated by eczema area and severity index (EASI) in patients. Skin specimens and vein blood samples were obtained from all the subjects. Reverse-transcription PCR was performed to detect the mRNA expression of IL-18 and IFN-γ in the skin tissue, and enzyme linked immunosorbent assay (ELISA) to measure the protein expression of IL-18 and IFN-γ in the sera of these subjects. Results The mRNA expression level in patients and controls was 1.04±0.29 pg/mL and 0.52±0.15 pg/mL for IL-18, respectively, 0.96±0.34 pg/mL and 0.47±0.12 pg/mL for IFN-γ, respectively; a significant increase was observed in the mRNA expression level of both IFN-γ and IL-18 in the patients than in the controls (both P<0.01). Moreover, the mRNA expression level of both IFN-γ and IL-18 positively correlated with the severity of eczema in patients (r=0.737, 0.883, both P<0.01). The protein expression level of IL-18 and IFN-γ was 475.8±59.4 pg/mL and 10.1±7.0 pg/mL, respectively, in the patients, 123.6 ±29.5 pg/mL and 11.1±3.4 pg/mL, respectively, in the controls; a statistical difference was observed in the protein expression level of IL-18 (P<0.01), but not in that of IFN-γ(P>0.01), between the patients and controls. No significant correlation was observed betweenthe serum level of IL-18 or IFN-γ and sererity of eczema in the patients (both P>0.01). Conclusions IL-18 may be involved in the pathogenesis of chronic eczema. Also, in local lesions, IL-18 seems to correlate with the induction of production of Th1 type cytokines, such as IFN-γ which could subsequently mediate hypersensitivity response.