Bcl-2 family plays an important role in cell apoptosis pathway. The ratio of pro-apoptosis members and anti-apoptosis members closely correlates with tumorigenesis, tumor radiosensitivity and progno-sis. Recent years ,according to the correlation of Bcl-2 family and tumor radiosensitivity, many researchers want to find new therapeutic strategies that target Bcl-2 in clinical radiotherapy.

Objective To investigate the practicality and stability of self -made bedside angle instrument in clinic .Methods 81 cases of mechanical ventilation were randomly divided into the experimental group (n=42) and the control group(n=39) .The bed head in the control group was elevated according to the experience and sensation .However the bed head in the experimental group was elevated by the self-made bedside angle instrument .The bed head elevation angle in the two groups was measured at the differ-ent timepoints .Results The accuracy of bed head elevation was 100% in the experimental group and 57 .69% in the control group , the difference between the two groups had statistical significance (P<0 .05) .Conclusion The bedside angle instrument has good practicality and stability ,effectively ensures the accuracy of the bed head elevation and contributes to conduct the body posture nursing on the patients with mechanical ventilation .

Objective To investigate the effects of different doses of propofol on cognitive function after chronic cerebral ischemia-induced injury in aged rats. Methods Eighty male SD rats, aged 18 months, weighing 400-500 g, were randomly divided into 4 groups ( n = 20 each): shame operation group (group S), chronic cerebral ischemia group (group I), two propofol groups (groups P1 and P2 ). The chronic cerebral ischemia was induced by permanent occlusion of bilateral common carotid arteries. On 1 day after operation, intraperitoneal normal saline 2.5 ml was injected twice a day for7 consecutive days in groups S and I, and intraperitoneal propofol 10 and 50 mg/kg in 2.5 ml of normal saline were injected twice a day for 7 consecutive days in groups P1 and P2 respectively. On 3rd and 33rd days after the last injection (T1.2), 10 rats in each group underwent Morris water maze test to assess the cognitive function. After the test was completed, the rats were sacrificed and the hippocampi were removed and sliced (450-500 μm thick). Schaffer lateral branch in CA1 region was stimulated to induce long-term potentiation (LTP). Results Compared with group S, the escape latency was significantly prolonged, the number of animals' swimming across the platform, the ratio of the swimming time spent in the forth quadrant to the total swimming time, and the success rate of LTP induction were significantly decreased at T1 and T2 in groups I, P1 and P2 (P ＜ 0.05). Compared with group I, the escape latency was significantly prolonged, the number of animals' swimming across the platform, the ratio of the swimming time spent in the forth quadrant to the total swimming time, and the success rate of LTP induction were significantly decreased at T1 in groups P1 and P2, and at T2 in group P2 ( P ＜ 0.05). Conclusion Propofol aggravates the damage to cognitive function while it attenuates the chronic cerebral ischemia-induced injury in aged rats, especially the high dose.

Objective To investigate the Shenmai injection for the inhibition of hepatic and renal toxicity and leukocyte disorder during chemoradiotherapy in the women with advanced breast cancer. Methods 58 cases of female breast cancer patients with stage Ⅳ were selected and randomly divided into 2 groups, 29 cases of each group, and patients were treated with 5-hydroxytryptamine (5-HT3) receptor antagonists and white blood cell growth hormone and other conventional therapy, the control group received gemcitabine plus cisplatin chemotherapy, 28d for 1 cycles, the treatment group received more with Shenmai injection, interval was 15d, 2 groups were treated for 3 cycles. Levels of peripheral blood T lymphocyte subsets, cytokine levels and liver and kidney function, quality of life and clinical efficacy were compared. Results Compared with before treatment, levels of CD3+, CD4+ and CD4+/CD8+ in control group decreased (P<0.05), levels of CD3+, CD4+ and CD4+/CD8+ in treatment group increased (P<0.05), levels of CD8+ decreased(P<0.05), levels of IFN-γ, IL-2 and TNF-α increased(P<0.05), levels of IL-6 decreased(P<0.05), scores of KPS increased(P<0.05), scores of FACT-B decreased(P<0.05), levels of ALT, AST, BUN increased(P<0.05), and levels of CCr, WBC counts decreased(P<0.05), and compared with the control group, levels of CD3+ , CD4+ and CD4+/CD8+ in the treatment group were higher(P<0.05), levels of CD8+ were lower(P<0.05), levels of IFN-γ, IL-2 and TNF-α were higher(P<0.05), levels of IL-6 were lower(P<0.05), and the total efficiency was higher(P<0.05), levels of ALT, AST, BUN were lower (P<0.05), and levels of CCr, WBC counts were higher (P<0.05). After treatment, the efficacy of treatment group was higher than that of control group(Z=-2.142,P=0.032<0.05). Conclusion Shenmai injection can improve the efficacy of radiotherapy and chemotherapy in patients with advanced breast cancer, and it can effectively inhibit the liver and kidney damage and leukocyte disorder.

Purpose [WT5”BZ]To estabalish a quantifying model of retinal neovascularization suitable for the study of pathogenesis and therapeutic intervention for the retinal neovascularization. [WT5”HZ]Methods [WT5”BZ]Sixteen one week old C 57 BL/6 mice were exposed to 75% oxygen for 5 days and then to room air and 16 mice of the same age kept in room air as controls.Ink perfused retinal flatmount was examined to assess the oxygen induced changes of retinal vessels.The proliferated neovascular response was quantitated by counting the nuclei of endothelial cells of new vessels extending from the retina into the vitreous in 6 ?m sagittal cross sections. VEGF and bFGF were determined on the cross sections after immunohistochemcal stain. [WT5”HZ]Results [WT5”BZ]Constriction and closure of the blood vessels were found under the hyperoxia condition,and dilation and proliferation were found under the relatively hypoxia status.There was a mean of 24 neovascular nuclei per cross section in the oxygen treated retina and less than 1 nucleus in the control group ([WT5”BX]P

Objective To investigate the possible mechanism of direct relaxant effects of propofol on tracheal smooth muscle.Methods Nine Japanese long-ear white rabbits of either sex, weighing 2-3 kg were sacrificed by air embolism. The trachea of each rabbit was removed and cut into six strips of (6-8) mm x (12-15) mm. The tracheal strips were suspended in Kreb' s solution, exposed to 95 % O2 and 5 % CO2 mixture and maintained at 37℃. One end of strip was fixed and the other was connected to a tension monitor through a tension transducer. The experiment was divided into six groups: group I propofol; group Ⅱ propofol + indothelin; group Ⅲp Ⅴ propofol + verapamil and group Ⅵ propofol + glibenclamide. In each group the tracheal strips were precontracted with acetylcholine (10-5 mol? L-1). The strips were then exposed to increasing concentrations of propofol (5 ? 10-3 , 10-2 ,2.5 ? 10-2 , 5 ? 10-2 , 10-1 mg?ml-1 ). At each concentration of propofol, the change in muscle tension was allowed to reach the steady state for 30 min. In group I no other drug was added to the Kreb' s solution besides propofol, while in group Ⅱ-Ⅵ before the effects of propofol were determined, the strips were pretreated with indothelin (10-4 mol? ml-1 ) (in group Ⅱ), L-NAME(10-4 mol?ml-1) (in group Ⅲ), methylene blue (10-5 mol?ml-1) (in group Ⅳ), verapamil (10-5 mol?ml-1) (in group Ⅴ) or glibenclamide (10-5 mol?ml-1) (in group Ⅵ). Results The three concentrations of propofol examined (2.5?10-2, 5?10-2 and 10-1 mg?ml-1) produced relaxation of isolated tracheal smooth muscle. In group Ⅱ , Ⅲ , Ⅳand Ⅴ pretreatment of tracheal strips with indothelin, L-NAME, methylene blue or verapamil did not significantly affect the relaxation of tracheal smooth muscle induced by propofol as compared with group Ⅰ. Pretreatment with glibenclamide significantly depressed the direct relaxant effect of propofol on tracheal smooth muscle ( P

The sectional morphology of the human brachial artery, ulnar artery, radial artery, palmar digital artery of the thumb and the middle finger were observed under microscopy. Excepting the brachial artery, the intima of the other 4 arteries are thick, especially the ulnar artery. The histological variations must be noticed during the artery is anastomosed. The percentage of the adventitia of the whole wall thickness decreases and the elastic fibers become looser gradually in accordance with the size of the arteries. The relative contents of elastin, collagen and smooth muscle of the above 5 arteries were measured by microspectrophotometer. The content of elastin of these arteries decreases(P

Objective: To observe the effects of taurine (Tau) on photoreceptor apoptosis and investigate the mechanism. Method: Seventy rats were randomly divided into control group (Cont) and 4% taurine supplementation group (Tau). The subjects were exposed to the cool white light (3000?200 lx)for 0, 1, 3, 6, 9, 12 or 24 h . The apoptotic index (AI) of photoreceptor was evaluated by TUNEL method. Meanwhile, levels of p65 in nuclear and caspase-1 were determined by Western-blot analysis and I?Ba mRNA was detected by RT-PCR . Results: After 9 h exposure, scattered TUNEL positive photoreceptors were found in Tau group, and each AI was lower than the corresponding control (P

Liver perfusion technique has been used in drug study for many decades. Liver perfusion has outstanding advantages over other techniques, such as isolated hepatocytes, hepatic cell lines, and hepatocyte membrane vesicles.It is an ideal experimental model used in drug metabolism, drug-drug interactions, and pharmacokinetic studies. Liver perfusion technique, its advantages or disadvantages, and its extensive applications have been reviewed.