Bcl-2 family plays an important role in cell apoptosis pathway. The ratio of pro-apoptosis members and anti-apoptosis members closely correlates with tumorigenesis, tumor radiosensitivity and progno-sis. Recent years ,according to the correlation of Bcl-2 family and tumor radiosensitivity, many researchers want to find new therapeutic strategies that target Bcl-2 in clinical radiotherapy.

Objective To investigate the practicality and stability of self -made bedside angle instrument in clinic .Methods 81 cases of mechanical ventilation were randomly divided into the experimental group (n=42) and the control group(n=39) .The bed head in the control group was elevated according to the experience and sensation .However the bed head in the experimental group was elevated by the self-made bedside angle instrument .The bed head elevation angle in the two groups was measured at the differ-ent timepoints .Results The accuracy of bed head elevation was 100% in the experimental group and 57 .69% in the control group , the difference between the two groups had statistical significance (P<0 .05) .Conclusion The bedside angle instrument has good practicality and stability ,effectively ensures the accuracy of the bed head elevation and contributes to conduct the body posture nursing on the patients with mechanical ventilation .

Objective To investigate the effects of different doses of propofol on cognitive function after chronic cerebral ischemia-induced injury in aged rats. Methods Eighty male SD rats, aged 18 months, weighing 400-500 g, were randomly divided into 4 groups ( n = 20 each): shame operation group (group S), chronic cerebral ischemia group (group I), two propofol groups (groups P1 and P2 ). The chronic cerebral ischemia was induced by permanent occlusion of bilateral common carotid arteries. On 1 day after operation, intraperitoneal normal saline 2.5 ml was injected twice a day for7 consecutive days in groups S and I, and intraperitoneal propofol 10 and 50 mg/kg in 2.5 ml of normal saline were injected twice a day for 7 consecutive days in groups P1 and P2 respectively. On 3rd and 33rd days after the last injection (T1.2), 10 rats in each group underwent Morris water maze test to assess the cognitive function. After the test was completed, the rats were sacrificed and the hippocampi were removed and sliced (450-500 μm thick). Schaffer lateral branch in CA1 region was stimulated to induce long-term potentiation (LTP). Results Compared with group S, the escape latency was significantly prolonged, the number of animals' swimming across the platform, the ratio of the swimming time spent in the forth quadrant to the total swimming time, and the success rate of LTP induction were significantly decreased at T1 and T2 in groups I, P1 and P2 (P ＜ 0.05). Compared with group I, the escape latency was significantly prolonged, the number of animals' swimming across the platform, the ratio of the swimming time spent in the forth quadrant to the total swimming time, and the success rate of LTP induction were significantly decreased at T1 in groups P1 and P2, and at T2 in group P2 ( P ＜ 0.05). Conclusion Propofol aggravates the damage to cognitive function while it attenuates the chronic cerebral ischemia-induced injury in aged rats, especially the high dose.

Objective To analyze the clinical effect of both micro-implant anchorage and selective extracting maxillary first molar to correct the failure of orthodontics.Methods From January 2006 to June 2012,24 cases who suffered from failure of orthodontics were treated in this department.To all patients,bilateral maxillary first molars were extracted,and micro-implant anchorage were planted into maxilla from buccal alveolar ridge to correct malocclusion acting as anchorages.Lateral cephalometric radiographs,intraoral photograph and facial photograph of every patient were taken before and after treatments; Their over bite and overjet molar relationship and incisors retraction were compared before and after treatments.Methods All the micro implant anchorages were sucessfuly implanted except two patients who experienced second operation owing to luxation of three implants.All the molars were strengthed and the profile improved along with their normal over bite and overjet.Conclusions The treatment of using micro-implant anchorage and selective extracting maxillary first molar is an ideal option of second orthodontics.Unconventional tooth extraction is used in this treatment.Micro implant anchorages are stable,acceptable and effective in clinical application.

The sectional morphology of the human brachial artery, ulnar artery, radial artery, palmar digital artery of the thumb and the middle finger were observed under microscopy. Excepting the brachial artery, the intima of the other 4 arteries are thick, especially the ulnar artery. The histological variations must be noticed during the artery is anastomosed. The percentage of the adventitia of the whole wall thickness decreases and the elastic fibers become looser gradually in accordance with the size of the arteries. The relative contents of elastin, collagen and smooth muscle of the above 5 arteries were measured by microspectrophotometer. The content of elastin of these arteries decreases(P

In recent years,nuclear medical imaging has become a routine examining technology.In general,angiographic medicine has to be injected into human body.Considering the short-coming of the manual injection,it seriously needs a injecting pump with the functions such as auto-control,push-pull injecting,synchronous injection of two injectors.

Objectives: Use antisense VEGF to reduce the immunosuppression induced by tumor and enhance the antithumor effect of IL-2. Methods: After the MMT45.Li murine liver cancer cells were modified with antisense VEGF alone, or antisense VEGF combined with IL-2 gene, the tumorigenesis of modified MM45T.Li was studied. The apoptosis of cancer cell induced by antisense VEGF and IL-2 gene in vivo was also studied. The tumors were cryosmeared 3 weeks after the mice bearing tumor were treated correspondingly with Ad-antiVEGF or Ad-Il-2 or Ad-antiVEGF/IL-2 or Ad-lacZ, the immunohistochemical analysis of CD4 + , CD8 + was performed.Results: The tumorigenesis of MM45T. Li was reduced by antisense VEGF, and the number of CD4 + , CD8 + cell was increased in tumor tissue, and the immunosupression was also reduced; At the same time antisense VEGF also enhanced the antithumor effect of IL-2 gene.Conclusions: Antisense VEGF not only can inhibit the neovascularization, but also can reduce the immunosupression induced by tumor, and improve lymphocyte infiltration toward tumor site;when it was combined with Il-2,anti VEGF can enhance the antitumor effect greatly.

Objective To investigate the Shenmai injection for the inhibition of hepatic and renal toxicity and leukocyte disorder during chemoradiotherapy in the women with advanced breast cancer. Methods 58 cases of female breast cancer patients with stage Ⅳ were selected and randomly divided into 2 groups, 29 cases of each group, and patients were treated with 5-hydroxytryptamine (5-HT3) receptor antagonists and white blood cell growth hormone and other conventional therapy, the control group received gemcitabine plus cisplatin chemotherapy, 28d for 1 cycles, the treatment group received more with Shenmai injection, interval was 15d, 2 groups were treated for 3 cycles. Levels of peripheral blood T lymphocyte subsets, cytokine levels and liver and kidney function, quality of life and clinical efficacy were compared. Results Compared with before treatment, levels of CD3+, CD4+ and CD4+/CD8+ in control group decreased (P<0.05), levels of CD3+, CD4+ and CD4+/CD8+ in treatment group increased (P<0.05), levels of CD8+ decreased(P<0.05), levels of IFN-γ, IL-2 and TNF-α increased(P<0.05), levels of IL-6 decreased(P<0.05), scores of KPS increased(P<0.05), scores of FACT-B decreased(P<0.05), levels of ALT, AST, BUN increased(P<0.05), and levels of CCr, WBC counts decreased(P<0.05), and compared with the control group, levels of CD3+ , CD4+ and CD4+/CD8+ in the treatment group were higher(P<0.05), levels of CD8+ were lower(P<0.05), levels of IFN-γ, IL-2 and TNF-α were higher(P<0.05), levels of IL-6 were lower(P<0.05), and the total efficiency was higher(P<0.05), levels of ALT, AST, BUN were lower (P<0.05), and levels of CCr, WBC counts were higher (P<0.05). After treatment, the efficacy of treatment group was higher than that of control group(Z=-2.142,P=0.032<0.05). Conclusion Shenmai injection can improve the efficacy of radiotherapy and chemotherapy in patients with advanced breast cancer, and it can effectively inhibit the liver and kidney damage and leukocyte disorder.

The formation of malignant ascites is concerned with non-obstructive factors including immunity regulator(IL-2,TNF,IFN-?)and factors of inducing blood vessel penetrability(VEGF,MMP) besides obstructive factors. Intraperitoneal chemotherapy makes drug directly act on peritoneum and arrives at the aim of controlling and treating malignant ascites.