No abstract available.
Antibodies, Monoclonal* ; Humans*

Objective To investigate the intracellular mechanisms by which 3,6-dihydroxyflavone inducing apoptosis of HL-60 cells.Methods After the HL-60 cells were treated with 3,6-dihydroxyflavone at the concentration of 5.10,20 or 40 mol/L,the cells then were inoculated under the selected optimal concentration.The survival rate of HL-60 cells was analyzed using a haemocytometer with standard trypan blue dye exclusion and a CASY cell counter and analyzer.The contents of malondialdehyde(MDA)and the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in the cells were measured by chemochromatometry.The expression of phosphorylation of ERK,JNK and p38MAPK were examined by Western blotting.Results The survival rate of HL-60 cells was significantly reduced in a dose-and time-dependent manner after 3,6-dihydroxyflavone treatment.When the dose was over 10 mol/L or the time was longer than 12 h under the concentration of 20 mol/L,significantly decreased survival rate was observed(P

INTRODUTION: The purposes of this study was to examine physical function and psychological status in the elderly caused by a fall. METHOD: The study was designed as a descriptive survey. Its subjects consisted of the 299 elderly over 65 years. Collected data were analyzed by the SPSS 10.0 program package. RESULTS: The result of this study are as follows : 1) The score for ADL was significantly lower in the fall group. 2) Grip strength was significantly lower in the fall group. 3) Lapse of the motion sitting and standing was significantly longer in the fall group. 4) One-leg-standing time with the eyes open and closed was significantly shorter in the fall group. 5) Return time of 3m walk was significantly longer in the fall group. 6) The score for dizziness was significantly higher in the fall group. 7) The score for fear for a fall and depression were significantly higher in the fall group. 8) Thescore for falls efficacy was significantly lower in the fall group. CONCLUSION: ADL, muscle strength, balance, dizziness, fear for a fall, falls efficacy and depression turned out to be closely related to the fall of the elderly. Therefore, it would be required to develop and applicate the fall prevention program regarding these above risk factors.
Activities of Daily Living ; Aged* ; Depression ; Dizziness ; Hand Strength ; Humans ; Muscle Strength ; Risk Factors

No abstract available.
Cause of Death* ; Korea*

We report metastatic omental hepatocellular carcinoma in two patients with post-lobectomy hepatocellular carcinoma who have had previous abdominal surgery or the rupture of hepatocellular carcinoma. Ometal metastatic masses were similar to primary masses of the liver in pathologic findings as well as in radiologic findings.
Carcinoma, Hepatocellular* ; Humans ; Liver ; Rupture

No abstract available.

In view of the potential of replicase protein as a diagnostic reagent for human caliciviruses (HuCVs), we have cloned and over-expressed this gene from the Snow Mountain-like Korean strains in Escherichia coli as a fusion protein with glutathione S-transferase (GST, and described the preliminary antigenic characterization of the recombinant products. Each 470bp fragment corresponding to highly conserved region of RNA-dependent RNA polymerase was generated by RT-PCR from stools of two diarrheal children, cloned in pMOSBlue T-vector, and subcloned between the EcoRI and SalI restriction sites of pGEX-47-3, a GST gene fusion vector, yielding pGCVpol. This construct expressed a Snow Mountain-like HuCV replicate under the control of the IPTG-inducible pac promoter. An extract prepared by sonication of the E. coli cell inclusion bodies bearing pGCVpol products was purified and analyzed by SDS-PAGE. After Coomassie blue staining, it was shown that the recombinant replicase migrated on the gels with an approximate molecular mass of 46.5 kDa, that was subsequently cleaved into a 26 kDa GST fragment and a 20.5 kDa replicase protein upon digestion with thrombin protease. The replicase was recognized on immunoblotting with the sera from symptomatic children with the HuCV-associated diarrhea but not by asymptomatic sera from adults. The results presented the first biological activity of individually expressed HuCV replicase subunit and provided important reagents for diagnosis of HuCV infection.
Adult ; Child ; Clone Cells ; Diagnosis ; Diarrhea ; Digestion ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; Gels ; Gene Fusion ; Glutathione Transferase ; Humans ; Immunoblotting ; Inclusion Bodies ; Indicators and Reagents ; RNA Replicase ; Snow* ; Sonication ; Staphylococcal Protein A ; Thrombin

Irritant or allergic contact dermatitis presents usually eczematous process or occasionally noneczematous lesions like in this case. We report a case of erythema rnultiforme-like eruptions in a 40-year-old female who presented multiple iris or annular shaped maculopapular eruptions on both hands and forearms after contact of lacquer tree. Being performed with standard patch test screening series, lacquer tree extracts and urushiol, she showed significant reactions to the lacquer tree and urushiol. Histopathologic findings showed both features of erythema multiforme and suh acute contact dermatitis.
Adult ; Dermatitis, Allergic Contact ; Dermatitis, Contact ; Erythema Multiforme* ; Erythema* ; Female ; Forearm ; Hand ; Humans ; Iris ; Lacquer* ; Mass Screening ; Patch Tests ; Trees*

We report herein a case of dilated pore occuring on the right philtrum of a, 65-year -old man. Histopathologic findings revealed irregular hyperplasia of the follicular wall focally in the lower portion but slightly atrophic in the ostial portion. This was treated by excision.
Hyperplasia ; Lip