Objective To investigate the synergistic effects of Herceptin and 9-cis-RA on the proliferation, cell cycle and apoptosis of HER2-positive breast cancer cells. Methods MDA-MB-453 breast cancer cells were treated with 5 ?g/ml Herceptin or 1 ?mol/L 9-cis-RA or both for 24, 48 or 72 h. The proliferation of MDA-MB-453 breast cancer cells was determined by MTT, the pro-apoptotic effects were detected by TUNEL, the cell cycle phase was detected by FCM. Results As compared with control group, Herceptin and 9-cis-RA synergistically inhibited the proliferation of MDA-MB-453 breast cancer cells, and the percentage of G_ 0 /G_ 1 cells increased after treatment with Herceptin and 9-cis-RA for 72 h. Simultaneously, Herceptin and 9-cis-RA synergistically induced the apoptosis of MDA-MB-453 cells. Conclusion Herceptin and 9-cis-RA could synergistically and effectively inhibit MDA-MB-453 breast cancer cells by blocking their cell cycle progression and inducing their apoptosis.

This study was aimed to clone the full-length cDNA sequence of actin gene of Eleutherococcus sentico-sus. And bioinformatics analysis was used. The total RNA was isolated from leaves of E. senticosus , and cDNA was synthesized by reverse transcription of total RNA. Primers were designed according to the conserved se-quence that had been cloned of Actin of E. senticosus . Then, the 3'and 5' cDNA fragments were cloned by nested PCR . The full-length gene was obtained by gene splicing method . Sequencing results were compared and treated with similarity analysis by blast analysis in the GenBank. Protein secondary structure and tertiary struc-ture of Actin of E. senticosus was predicted by online software. The results showed that the full-length cDNA of Actin of E. senticosus is 1507 bp, which named EsActin1, GenBank accession No. KC469585. The conserved sequence, which contained a 1134 bp open reading frame that encoding a 377 amino acid residues, a 5'-UTR of 140 bp and a 3'-UTR of 233 bp. Homologous alignment showed that it shared over 75% nucleotide se-quence similarity and over 94% amino acid sequences similarity with Actins in other plants. It was concluded that this study first isolated and reported the full-length cDNA sequence of actin gene of E. senticosus , and laid a foundation for the molecular biology research of E. senticosus .

Objective:To establish a method by combining microwave-assisted extraction(MAE),solid phase extraction(SPE)and high performance liquid chromatography(HPLC)for determination of the mefenacet residues in rice.Methods:Acetone and acetontrile(37)were used as extraction solvent.Microwave-assisted extraction was used to extract mefenacet residues in the rice.The extracts were then cleaned up with a Florisil cartridge and then subjected to Hypersil C18 column(5 ?m,4.6 mm?200 mm),with acetonitrilewater(5050,V/V)solution as mobile phase and with a flow rate of 1.0 ml/min;the ultraviolet detection wavelength was at 217 nm.Results:Good linear correlation for mefenacet was found within a concentration range of 0.198-9.900 ?g/ml.The detection limit was 0.039 6 ?g/mL for mefenacet(S/N=2).The average recovery rate of rice hull and brown rice were 90.8%(RSD 1.8%)and 85.6%(RSD 2.5%),respectively.Conclusion:The present method is simple and rapid;it can be used for the determination of mefenacet residues in rice.

No abstract available.
Krukenberg Tumor*

No abstract available.
Choriocarcinoma* ; Female ; Ovary* ; Pregnancy

OBJECTIVES: For the understanding and effective treatment of depressive symptoms in chronic schizophrenia, this study investigated the frequency of depressive symptoms and examined associations between depressive symptoms and positive symptoms, negative symptoms, general psychopathology in chronic schizophrenia. METHOD: The authors assessed the frequency of depressed schizophrenia with 30 or higher scores of HRSD in 135 DSM-IV chronic schizophrenia. We measured PANSS, BPRS in depressed(n=37) and non-depressed schizophrenia(n=37) who were matched in sex, age and dose of antipsychotics to compare positive, negative symptoms and other psychopathology. Also, we evaluated correlation between depressive symptoms and positive symptoms, negative symptoms, other psychopathology in depressed schizophrenic patients. RESULTS: 1) The depressive symptoms were present in 27.4% of chronic schizophrenia. 2) The positive scale of PANSS were significantly higher in depressed than in non-depressed schizophrenia(p<.01), and the negative scale of PANSS were higher in depressed schizophrenia but there were no statisical significance. Thinking disturbance and depressive-anxiety factors of BPRS were significantly higher in depressed than in non-depressed schizophrenia(p<.01, p<.01). 3) The positive and negative scale of PANSS correlated with HRSD in depressed schizophrenia(p<.01, p<.05), and thinking disturbance and depressive-anxiety factors correlated with HRSD in depressed schizophrenia(p<.01, p<.01). CONCLUSION: The depressive symptoms are relatively commom and important part of schizophrenic symptomatology, and they are more related to positive symptoms than negative symptoms of schizophrenia. Thus this study suggest that appropriate assessment and therapeutic intervention for depressive symptoms is especially necessary to the schizophrenic patients with severe positive symptoms.
Antipsychotic Agents ; Depression ; Diagnostic and Statistical Manual of Mental Disorders ; Humans ; Psychopathology ; Schizophrenia* ; Thinking

PURPOSE: This study was designed to investigate the relationships of the types of nurse staffing, the competency of nursing performance and job satisfaction by analyzing the types of nurse staffing in operating room. METHOD: The subjects were 472 nurses who work in operating room. The instruments used in this study were three tools: competency of nursing performance, job satisfaction and type of nurse staffing. RESULT: Type 4 was most in the type of nurse staffing of the subjects. According to the type of nurse staffing, the competency of nursing performance was significantly different. There was significant difference in the competency of nursing performance according to age, marital status, bachelor, employ, position, and career, and job satisfaction according to age, position, and career. In opinion about the ideal type of nurse staffing, type 2 was most regardless of career. CONCLUSION: Type 4 which has lower competency of nursing performance was adopted in many hospitals now but, type 2 which has highest competency of nursing performance was selected by many nurses as the best one. So, it should be considered the type of nurse staffing of in operating room and change it.
Job Satisfaction* ; Marital Status ; Nursing* ; Operating Rooms*

The incidence of dextrocardia is lower. The dextrocardia is often associated with congenital anatomical abnormalities. The patient with normal cardiac structure of dextrocardia and severe coronary heart disease that needs coronary artery bypasss is rarer. This article summarizes the perioperative management and surgical experience of dextrocardia with bypass surgery via a clinical case.

PURPOSE: This study was conducted to determine the influence of ego-identity, parenting efficacy, and stress coping style on depression in mothers of toddlers. METHODS: This was a cross-sectional, descriptive study. Data were collected from 164 mothers of toddlers, and analyzed using descriptive statistics, the t-test, analysis of variance, Pearson correlation coefficients, and multiple regression analysis using SPSS for Windows version 24.0. RESULTS: The factors influencing depression in mothers of toddlers included ego-identity, economic status, and perceived health status. These variables explained 60.0% of depression in mothers of toddlers. CONCLUSION: Lower ego-identity was associated with more intense depression. Therefore, healthcare providers should develop intervention programs that can improve ego-identity to decrease depression among mothers of toddlers.
Child, Preschool ; Depression ; Health Personnel ; Humans ; Mothers ; Parenting ; Parents

PURPOSE: Quality of life is important to everyone including school-age children. Therefore this study was done to identify the effects perceived daily stress and sense of humor on quality of life for these children. METHODS: The study was a cross-sectional, descriptive study design with 371 participants from 5th and 6th grade children in 5 elementary schools. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation coefficients, and multiple regression analysis with SPSS/WIN 24.0 program. RESULTS: Factors influencing quality of life in school-age children were daily stress, sense of humor, satisfaction with daily life, academic performance and home atmosphere. These variables explained 63.0% of quality of life in school-age children. CONCLUSION: Results showed that lower daily stress and higher sense of humor are associated with higher quality of life. Therefore, to improve quality of life in school-age children, healthcare providers should develop intervention programs considering these factors.
Atmosphere ; Child ; Health Personnel ; Humans ; Quality of Life