1.Retrospective and cost-effective analysis of the result of Changsha Municipal Public Welfare Program by Noninvasive Prenatal Testing.
Dongbo WANG ; Jun HE ; Yuting MA ; Hui XI ; Meng ZHANG ; Haixia HUANG ; Lijuan RAO ; Binbin ZHANG ; Chunmei MI ; Bo ZHOU ; Zhehui LIAO ; Lei DAI ; Xinyu OUYANG ; Yang ZHANG ; Haiyan WANG ; Xin WANG ; Zhaohui ZHANG ; Sui YAO ; Zhenyu TAN ; Jing YANG ; Wei ZHONG ; Nan WANG ; Jiyang LIU ; Liangrong ZHOU
Chinese Journal of Medical Genetics 2022;39(3):257-263
OBJECTIVE:
To assess the practical and health economical values of non-invasive prenatal test (NIPT) in Changsha Municipal Public Welfare Program.
METHODS:
A retrospective analysis was carried out on 149 165 women undergoing NIPT test from April 9, 2018 to December 31, 2019. For pregnant women with high risks, invasive prenatal diagnosis and follow-up of pregnancy outcome were conducted. The cost-benefit of NIPT for Down syndrome was analyzed.
RESULTS:
NIPT was carried out for 149 165 pregnant women and succeeded in 148 749 cases (99.72%), for which outcome were available in 148 538 (99.86%). 90% of pregnant women from the region accepted the screening with NIPT. 415 (0.27%) were diagnosed as high risk. Among these, 381 (91.81%) accepted amniocentesis, which led to the diagnosis of 212 cases of trisomy 21 (PPV=85.14%), 41 cases with trisomy 18 (PPV=48.81%) and 10 cases with trisomy 13 (PPV=20.83%). The sensitivity and specificity of NIPT for trisomy 21, trisomy 18 and trisomy 13 were (97.70%, 99.98%), (97.62%, 9.97%) and (100%, 99.97%), respectively. In addition, 213 and 30 cases were diagnosed with sex chromosomal aneuploidies (PPV=46.2%) and other autosomal anomalies (PPV=16.57%), respectively. For Down syndrome screening, the cost and benefit of the project was 120.79 million yuan and 1,056.95 million yuan, respectively. The cost-benefit ratio was 1: 8.75, and safety index was 0.0035.
CONCLUSION
NIPT is a highly accurate screening test for trisomy 21, which was followed by trisomy 18 and sex chromosomal aneuploidies, while it was less accurate for other autosomal aneuploidies. The application of NIPT screening has a high health economical value.
Aneuploidy
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Cost-Benefit Analysis
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Female
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Humans
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Noninvasive Prenatal Testing
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Pregnancy
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Retrospective Studies
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Trisomy 18 Syndrome/genetics*
2.Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus
Jia-ying SUN ; Zhen WANG ; Yao-lei MI ; Xiang-xiao MENG ; Hui-hua WAN ; Yun YAN ; Wei SUN ; Wei MA
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(5):124-131
Objective:To analyze the sequence characteristics,chromosomal location,gene structure,conserved motifs,phylogenetic evolution and differential gene expressions of the
3.Genome-wide analysis of LBD(lateral organ boundaries domain) gene family in Cannabis sativa of traditional Chinese medicine hemp seed.
Zhen WANG ; Mi YAO-LEI ; Xiang-Xiao MENG ; Hui-Hua WAN ; Ji AI-JIA ; Wei SUN ; Ma WEI
China Journal of Chinese Materia Medica 2020;45(22):5477-5486
LBD(lateral organ boundaries)transcription factors play an important role in the regulation of plant growth, development and secondary metabolism. In order to explore the function of LBD genes in cannabis, the Cannabis sativa genome and transcriptome were used to identify the C. sativa LBD gene family, and analyzed their expression patterns. Our results showed that the cannabis LBD contains 32 members, which were divided into two major categories, seven sub-families. Class Ⅰ was divided into 5 sub-families, named Class Ⅰ_a to Class Ⅰ_e, while Class Ⅱ was divided into 2 sub-families, including Class Ⅱ_a and Class Ⅱ_b. Analysis showed that the number of amino acids encoded LBDs was between 172 and 356, and the isoelectric point was between 4.92 and 9.43. The mole-cular weight of LBD was between 18 862.92 Da and 40 081.33 Da, and most members are located in the nucleus. Chromosome positioning of LBD showed that 32 members were unevenly distributed on 10 chromosomes of C. sativa LBD transcription factor domain, gene structure and motifs are relatively conservative, and the characteristics of different class members are similar. The upstream promoter region of the gene contains a variety of cis-acting elements related to plant hormones and environmental factors, C. sativa LBD genes have different expression patterns in the stems, leaves, and flowers of ZYS varieties(low tetrahydrocannabinol, high cannabidiol). The members of the LBD gene family are mainly expressed in the flowers and stems of ZYS varieties, while members expressed in the leaves very few; Class Ⅱ members CsLBD21 and CsLBD23 are expressed in flowers and stems, and CsLBD8 and CsLBD18 are expressed in flowers, stems and leaves. These genes may participate in the growth and development of cannabis and affect the biosynthesis of cannabinoids. This study laid the foundation for the subsequently functional research of the cannabis LBD gene family.
Cannabis/metabolism*
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Gene Expression Regulation, Plant
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Humans
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Medicine, Chinese Traditional
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Phylogeny
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Plant Proteins/metabolism*
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Seeds/metabolism*
4.Application of MALDI-mass spectrometry imaging in spatial distribution of secondary metabolites in medicinal plants -- a case study of Lepdium meyenii root.
Yao-Lei MI ; Wei SUN ; Ming-Li LI ; Hai-Yu ZHAO ; Bao-Lin BIAN ; Yan-Yan ZHOU
China Journal of Chinese Materia Medica 2020;45(3):596-601
Matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) is a novel technique for in-situ distribution of various substances in tissue without labeling. This technique is increasingly applied to the study of medicinal plants owing to its high spatial resolution and its potential of in-situ analysis in small molecules. In this study, the structural information and their fragmentation patterns of the midazole alkaloids(1,3-dibenzyl-4,5-dimethylimidazolium chloride and 1,3-dibenzyl-2,4,5-trimethylimi-dazolium chloride) and benzylglucosinolate in the medicinal plant Maca(Lepdium meyeni) root were analyzed by ultra-high-performance liquid phase combined with LTQ-Orbitrap mass spectrometry(UHPLC-HR-MS). The localization of these active ingredients in the cross-sections of Maca root was performed by MALDI-MSI. These results demonstrated that the two types of imidazole alkaloids had a similar distributed pattern. They were located more in the cortex and the periderm than those in the medulla of a lateral root, while the localization of benzylglucosinolate was concentrated in the center of the root rather than in the cortex and the periderm. The precise spatial distribution of various secondary metabolites in tissue provides an important scientific basis for the accumulation of medicinal plant active ingredients in tissues. In addition, this imaging method is a promising technique for the rapid evaluation and identification of the active ingredients of traditional Chinese medicine in plant tissues, as well as assisting the research on the processing of medicinal plants.
Chromatography, High Pressure Liquid
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Lepidium/chemistry*
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Phytochemicals/analysis*
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Plant Roots/chemistry*
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Plants, Medicinal/chemistry*
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Secondary Metabolism
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
5.Genome-wide Identification and Characterization of TIFY Gene Family in Medicinal Plant Cannabis sativa
Dong WEN ; Meng-yue WANG ; Yao-lei MI ; Wei MA ; Wei SUN ; Yu-hua SHI
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(24):134-143
Objective:The TIFY gene family will be identified and characterized from the whole genome level in
6.Identification and expression analysis of the C2H2 gene family in Cannabis sativa L.
Mei-qi LIU ; Wei SUN ; Xiang-xiao MENG ; Hui-hua WAN ; Ting-xia LIU ; Jia-ying SUN ; Zhen WANG ; Yao-lei MI ; Wei MA
Acta Pharmaceutica Sinica 2021;56(5):1486-1496
C2H2 transcription factors play an important role in plant growth, development and the regulation of secondary metabolism. This article identifies members of the
7.Advances in biosynthesis of indigo in plants.
Yi-Ming ZHANG ; Yuan-Zhen HUANG ; Hui-Hua WAN ; Ze-Xin LI ; Wei SUN ; Sha-Sha WU ; Jun-Wen ZHAI ; Yao-Lei MI
China Journal of Chinese Materia Medica 2020;45(3):491-496
Natural indigo, as one of the oldest dyes, is also a pivotal dye utilized in cotton fabrics today. A diversity of plants rich in indigo compounds belong to traditional Chinese herbal medicines. Indigo compounds have a variety of biological and pharmacological activities, including anticonvulsant, antibacterial, antifungal, antiviral and anticancer activities. A substantial progress in indigo biosynthesis has been made lately. This paper summarizes the value of indigo from the aspects of cultural history, biosynthetic pathways and the medicinal activities of its related derivatives involved in the pathways. In addition, the latest research advancements in indigo biosynthetic pathways is demonstrated in this paper, which would lay the theoretical foundation for the exploration and utilization of natural indigo.
Biosynthetic Pathways
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Coloring Agents
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Indigo Carmine/metabolism*
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Indigofera/metabolism*