Objective To develop a new flow-injection chemiluminescence(FI-CL) method for the determination of acyclovir and gancyclovir by using the CL system of Ce(IV)-Rhodamine B.Methods The redoxreaction of Ce(IV) and acyclovir/gancyclovir in H2SO4 medium could generate CL signal.Rhodamine B could obviously sensitize this signal,and the CL intensity was proportional to the concentration of acyclovir and gancyclovir.Therefore,a new FI-CL method has been described for the determination of acyclovir and gancyclovir.Results For acyclovir,the determination range was 3.0?10-5g/L-7.0?10-2g/L,with 1.56?10-5g/L as its determination limit.During 11 repeated measurements for 1.0?10-3g/L acyclovir,the relative standard deviation was 2.08%.For ganciclovir,the determination range was 5.0?10-5g/L-7.0?10-2g/L,with 2.35?10-5g/L as its determination limit.The relative standard deviation was 2.83%,with 11 repeated measurements of 1.0?10-3g/L ganciclovir.Conclusion This method has broad linear range,high sensitivity,and convenience and speediness,it therefore,can be successfully used to determine the content of ganciclovir in injections.

Objective: The high performance liquid chromatography diode array detection method was used to establish the determination methods for the contents of chlorogenic acid, rutin, quercetin, and luteolin in Kunlun snow daisy (Coreopsis tinctoria, Coreopsis Tinctoriae Folium). Methods: Agilent HC-C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was CH3OH (A)-0.01% H3PO4 (B) for the gradient elution with flow rate of 1.0 mL/min, the detection wavelength was 315 nm and the column temperature was 30 ℃. Results: There was a linear correlation between the concentration of chlorogenic acid 2.0-60 μg/mL (r = 0.999 8), rutin 5.0-100 μg/mL (r = 0.999 9), quercetin 1.0-40 μg/mL (r = 0.999 9), and luteolin 0.5-40 μg/mL (r = 0.999 9). The average recovery rates of chlorogenic acid, rutin, quercetin, and luteolin were 102.4% (RSD = 1.05%, n = 5), 98.6% (RSD = 1.28%, n = 5), 103.6% (RSD = 0.95%, n = 5), and 101.2% (RSD = 1.12%, n = 5), respectively. Conclusion: The analysis method is simple, rapid, reproducible, accurate, and reliable. It could be used to identify and evaluate the quantitative determination of chlorogenic acid, rutin, quercetin, and luteolin contents in Kunlun snow daisy.