1.Detection of periodontal disease related bacteria from the implant-abutment interface in oral cavity.
Meung Ju HAN ; Chae Heon CHUNG ; Hee Jung KIM ; Joong Ki KOOK ; So Young YOO
The Journal of Korean Academy of Prosthodontics 2008;46(2):116-124
STATEMENT OF PROBLEM: Implant systems result in gaps and cavities between implant and abutment that can act as a trap for bacteria and thus possibly cause inflammatory reactions in the peri-implant soft tissues. PURPOSE: Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, and Aggregatibacter actinomycetemcomitans, related to implant-abutment interface microleakage. MATERIAL AND METHODS: Samples were taken from 27 subjects with sterilized paper points and were transported in 1XPBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA. RESULTS: Our data showed that the detection rate of P. gingivalis and P. intermedia in implant fixture was 59% and 82% in patients respectively. Detection rate of P. gingivalis and P. intermedia in implant crevice was 44% and 82% in patients. Detection rate of P. gingivalis and P. intermedias in tongue was 82% and 82% in patients. CONCLUSION: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.
Bacteria
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Colon
;
DNA, Ribosomal
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Forsythia
;
Humans
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Mouth
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Periodontal Diseases
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Polymerase Chain Reaction
;
Porphyromonas gingivalis
;
Prevotella intermedia
;
Tongue
;
Treponema denticola
2.A Case of Chronic Neutrophilic Leukemia with Multiple Myeloma.
Kwang Hyeun KIM ; One Young KIM ; Cheol Hyeon JO ; Seung Oeuk LEE ; Bong Han YOON ; Myung Weon KANG ; Yeun Keun LIM ; Hyang Sun YEU ; Meung Hee LEE
Korean Journal of Hematology 1998;33(3):454-459
Chronic neutrophilic leukemia(CNL) is a rare disorder characterized by persistent neutrophilia of mature cell type without evidence of infection, hepatosplenomegaly, elevated neutrophil alkaline phosphatase score and the absence of Philadelphia chromosome We report a typical case of CNL with multiple myeloma(IgG-lambda type) in 68 years old female. Many neutrophils had toxic granule and vacuole. The peripheral blood smear showed neutrophlic leukocytosis(50,940/microliter, neutrophil 94%) with rare immature form. The cytogenetic study showed normal karyotype without Philadelphia chromosome. She was diagnosed as multiple myeloma with paraproteinemia, lytic bone lesion of skull, and immature plasma cells in bone marrow.
Aged
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Alkaline Phosphatase
;
Bone Marrow
;
Cytogenetics
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Female
;
Humans
;
Karyotype
;
Leukemia, Neutrophilic, Chronic*
;
Multiple Myeloma*
;
Neutrophils
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Paraproteinemias
;
Philadelphia Chromosome
;
Plasma Cells
;
Skull
;
Vacuoles