1.Effectiveness of Palatal Mucosa Graft in Surgical Treatment of Sub-Glottic Stenosis.
Umit AYDOGMUS ; Adem TOPKARA ; Metin AKBULUT ; Adem OZKAN ; Figen TURK ; Barbaros SAHIN ; Gokhan YUNCU
Clinical and Experimental Otorhinolaryngology 2016;9(4):358-365
OBJECTIVES: Mucosal free grafts may be successfully applied in many surgical interventions. This study aims at investigating the feasibility of palatal mucosa graft in sub-glottic field in an animal model. METHODS: This randomized prospective controlled study was conducted with an animal model. Sub-glottic inflammation was created in 15 adult rabbits in each group and sub-glottic stenosis surgery was applied thereafter. The rabbits in group 1 (control group) underwent segmental resection, partial cricoidectomy, and trachea-thyroid cartilage anastomosis; the rabbits in group 2 underwent segmental resection, cricoplasty, and crico-tracheal anastomosis using free buccal mucosa graft; and the rabbits in group 3 underwent segmental resection, cricoplasty, and crico-tracheal anastomosis using free palatal mucosa graft. Re-stenosis was evaluated after 42 days. RESULTS: The percentages of stenosis were 27%±20%, 40%±20%, and 34%±23% for group 1, 2, and 3, respectively and the difference was not statistically significant (P=0.29). Intensive and tight fibrosis was observed in 2 rabbits (13%) in group 1, in 5 rabbits (33%) in group 2, and in 3 rabbits (20%) in group 3. There was not a statistically significant difference between groups (P=0.41). Excessive inflammation was observed in 3 rabbits (20%) in group 1, in 7 rabbits (47%) in group 2, and 3 rabbits (20%) in group 3. There was no a statistically significant difference between groups although inflammation rate was higher in the rabbits which underwent buccal mucosa graft (P=0.18). CONCLUSION: The surgical treatments applied with free mucosa graft reduced anastomosis tension through enabling anastomosis to the distal of cricoid instead of thyroid cartilage. Free palatal mucosa grafts may be used in sub-glottic field, one of the most challenging fields of trachea surgery, due to ease of application and rapid vascularization.
Adult
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Autografts
;
Cartilage
;
Constriction, Pathologic*
;
Fibrosis
;
Humans
;
Inflammation
;
Models, Animal
;
Mouth Mucosa
;
Mucous Membrane*
;
Prospective Studies
;
Rabbits
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Thyroid Cartilage
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Trachea
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Tracheal Stenosis
;
Transplants*
;
Wound Healing
2.Serum Chitotriosidase Activity in Pulmonary Tuberculosis: Response to Treatment and Correlations with Clinical Parameters.
Gulhan CAKIR ; Seyfettin GUMUS ; Ergun UCAR ; Hatice KAYA ; Ergun TOZKOPARAN ; Emin Ozgur AKGUL ; Bulent KARAMAN ; Omer DENIZ ; Ismail KURT ; Metin OZKAN ; Hayati BILGIC
Annals of Laboratory Medicine 2012;32(3):184-189
BACKGROUND: Chitotriosidase is an accepted marker of macrophage activation. In this study, we investigated serum chitotriosidase levels in pulmonary tuberculosis (PTB). METHODS: Forth-two patients with PTB and 30 healthy subjects were enrolled in the study. The radiological extent of PTB, radiological sequela after treatment, and the degree of smear positivity were assessed. Chitotriosidase levels were measured by a fluorometric method. RESULTS: The serum chitotriosidase levels of the PTB patients were significantly higher than those of the control subjects (39.73+/-24.97 vs. 9.63+/-4.55 nmol/mL/h, P<0.001). After completion of the standard 6-month antituberculous treatment, chitotriosidase levels in PTB patients significantly decreased (10.47+/-4.54 nmol/mL/h, P<0.001). Chitotriosidase levels correlated significantly with the radiological extent of PTB, degree of smear positivity, and post-treatment radiological sequela score (r=0.439, r=0.449, and r=0.337, respectively). CONCLUSIONS: This study demonstrated that serum chitotriosidase levels increase in PTB; therefore, chitotriosidase can be used as a marker of disease activity, severity, and response to treatment.
Adult
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Antitubercular Agents/therapeutic use
;
Biological Markers/blood
;
Fluorometry
;
Hexosaminidases/*blood
;
Humans
;
Male
;
ROC Curve
;
Severity of Illness Index
;
Tuberculosis, Pulmonary/drug therapy/*enzymology/radiography
;
Young Adult
3.Clinicopathologic Characteristics and Therapeutic Outcomes of Primary Gastrointestinal Non-Hodgkin's Lymphomas in Central Anatolia, in Turkey.
Bulent ESER ; Bunyamin KAPLAN ; Ali UNAL ; Ozlem CANOZ ; Fevzi ALTUNTAS ; H Ismail SARI ; Ozlem ER ; Metin OZKAN ; Can KUCUK ; Makbule ARAR ; Sebnem GURSOY ; Mustafa CETIN
Yonsei Medical Journal 2006;47(1):22-33
Primary gastrointestinal lymphoma is a common presentation of non-Hodgkin's lymphoma. The main controversy arises when many aspects of its classification and management are under discussion, particularly regarding roles for surgical resection. The aim of this study was to evaluate clinicopathologic characteristics and the therapeutic outcome of primary gastrointestinal non-Hodgkin's lymphoma. We carried out a retrospective analysis of 74 patients who were presented to our center with histopathological diagnosis of primary gastro-intestinal non-Hodgkin's lymphoma between 1990 and 2001. All patients have been staged according to Lugano Staging System. For histopathological classification, International Working Formulation was applied. The treatment choice concerning the surgical or non-surgical management was decided by the initially acting physician. Treatment modalities were compared using the parameters of age, sex, histopathological results, stage, and the site of disease. Of the 74 patients, 31 were female and 43 were male, with a median age of 49 years (range 15-80). The stomach was the most common primary site and was seen in 51 of 74 patients (68.9%). The intermediate and high grade lymphomas constituted 91.9% of the all cases. In a median follow-up of 29 months (range 2-128), 20 out of 74 patients died. There was a three year overall survival rate in 65.4% of all patients. The three year overall survival rate was better in stage I and II1 patients who were treated with surgery plus chemotherapy (+/-RT) than those treated with chemotherapy alone (93.7% vs. 55.6%, p<0.05). The stage and presence of B symptoms affected the disease free survival and overall survival significantly, but the histopathologic grade only affected the overall survival. On the basis of these results, we suggest that surgical resection is necessary before chemotherapy in early stage (stage I and II1) patients with gastrointestinal non-Hodgkin's lymphomas because of the significant survival advantage it would bring to the patient.
Turkey/epidemiology
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Treatment Outcome
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Survival Rate
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Retrospective Studies
;
Neoplasm Staging
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Middle Aged
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Male
;
Lymphoma, Non-Hodgkin/mortality/*pathology/*therapy
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Humans
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Gastrointestinal Diseases/mortality/*pathology/*therapy
;
Female
;
Combined Modality Therapy/adverse effects
;
Aged, 80 and over
;
Aged
;
Adult
;
Adolescent
4.Evaluation of oxidative stress and inflammation in patients with polycystic ovary syndrome
Berra SEN ; Senem GONULTAS ; Ceren ALBAYRAK ; Sevval TEMUR ; Ilgar ACAR ; Beyza Nur OZKAN ; Havva Sevde ISLEK ; Muserref Banu YILMAZ ; Ebru KALE ; Eray Metin GULER
Obstetrics & Gynecology Science 2024;67(4):414-420
Objective:
Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine and metabolic disorder characterized by hyperandrogenism, hyperinsulinemia, and insulin resistance. The prevalence of PCOS is increasing worldwide. Although the etiology of this disease is currently unknown, it is thought to be closely related to inflammation and oxidative stress. Our study aimed to compare patients have PCOS to healthy volunteers and assess the changes in oxidative stress and inflammatory parameters in these patients.
Methods:
Thirty patients between the ages of 18-45 diagnosed with PCOS and 30 healthy volunteers with the same demographic characteristics were included in this study. Clinical parameters were measured using immunoassays. Oxidative stress biomarkers, total oxidant (TOS), total antioxidant (TAS), total thiol (TT), and native thiol (NT) levels were measured using photometric methods according to Erel’s method. The dynamic disulfide level (DIS) and oxidative stress index (OSI) were calculated using mathematical equations. Among the inflammatory parameters, values for interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) were measured photometrically using commercially purchased kits.
Results:
Moreover, TT and NT levels were lower in patients with PCOS compared to those in the healthy group statistically significantly (P<0.001). In addition, TAS, TOS, OSI, DIS, IL-1β, IL-6, and TNF-α levels were identified to be significantly higher in the patients with PCOS than those in the healthy group (P<0.001).
Conclusion
Evaluation of oxidative stress and clinical parameters used in the follow-up may be beneficial for the disease.