Chromatography, Gas ; Humans ; Methylene Chloride ; urine

Animals ; Liver ; drug effects ; Methylene Chloride ; toxicity ; Mice ; Oxidative Stress

Air ; analysis ; Chromatography, Gas ; methods ; Methylene Chloride ; analysis ; Workplace

STATEMENT OF PROBLEM: The failure of adhesion between the resilient denture liner and the denture base is a serious problem in clinic. PURPOSE: The PURPOSE of this study was to evaluate the effect of denture base resin surface pretreatments (mechanical and/or chemical) on the tensile bond strength between a resilient liner and processed denture resin. MATERIAL AND METHOD: Acrylic-based resilient liners (Soft liner; GC co., Japan & Coe-Soft; GC America Inc. USA) and silicone-based resilient liners (Mucosoft; Parkell Inc., USA & Dentusil; Bosworth co., USA) were used. Specimens in each soft lining material were divided two groups with or without mechanical pretreatment. Each denture base specimen received 1 of 4 chemical pretreatments including: (1) no treatment, (2) 30-s acetone treatment, (3) 15-s methylene chloride treatment, (4) 180-s methyl methacrylate treatment. All specimens were thermocycled and placed under tension until failure in a universal testing machine. RESULTS: 1. Silicone-based resilient liners exhibited significantly higher tensile bond strengths than acrylicbased resilient liners (P<.05). 2. Grinding the denture base resin improved tensile bond strengths of silicone-based resilient liners, but reduced tensile bond strengths of acrylic-based resilient liners (P<.05). 3. In acrylic-based resilient liners, treating with acetone significantly increased the bond strength of Soft liner and treating with methyl methacrylate significantly increased the bond strength of Coe-Soft (P<.05). However they were not effective compared to silicone-based resilient liner. 4. In silicone-based resilient liners, treating with all chemical etchants significantly increased the bond strength of Mucosoft to denture base, and treating with methylene chloride and methyl methacrylate increased the bond strength of Dentusil to denture base (P<.05). CONCLUSION: Although chemical and mechanical pretreatments were not effective on tensile bond strength of acrylic-based resilent liner to denture base, treating the denture base resin surface with appropriate chemical etchants after mechanical pretreatment significantly increased the tensile bond strength of silicone-based resilient liner to denture base.
Acetone ; Americas ; Denture Bases* ; Denture Liners ; Dentures* ; Japan ; Methylene Chloride

Air ; analysis ; Chromatography, Gas ; methods ; Ethylene Oxide ; analysis ; Furans ; analysis ; Methylene Chloride ; analysis ; Workplace

Adult ; Humans ; Methylene Chloride ; adverse effects ; Neurotransmitter Agents ; blood ; Occupational Exposure ; adverse effects

OBJECTIVES: The authors report a case of pneumonitis caused by methylene chloride (MC). METHODS: The patient was examined and diagnosed by clinical, radiologic, laboratorial and medical evaluation. The concentration of MC in the factory was measured to determine the exposure level. RESULTS: The patient had an infiltration in chest X-ray, which underwent rapid relief of radiological finding, respiratory acidosis and hypoxemia in arterial blood gas analysis, and a ground-glass opacity in high resolution computerized tomography. MC was the only exposed chemical compound in this case. The concentration of MC was 39.9 ppm in the factory. CONCLUSIONS: We diagnosed this to be a case of pneumonitis by MC based on clinical laboratory findings, exposure history to MC and MC concentration in the factory.
Acidosis, Respiratory ; Anoxia ; Blood Gas Analysis ; Humans ; Methylene Chloride* ; Pneumonia* ; Thorax

The ideal goal of periodontal therapy is the regeneration of periodontal tissue and repair of function. Although it is very difficult to attain this goal, recent advances in periodontal wound healing concepts encourage hope reaching it. Recently many efforts are concentrated on the regeneration potential of material used in traditional Korean medicine. Phlomidis Radix has been used for the treatment of blood stasis, bone fracture and osteoporosis in traditional Korean medicine. The purpose of this study is to examine effects of dichloromethane fraction Phlomidis Radix on Bone Formation in Human Fetal Osteoblasts. Human fetal osteoblastic cell line(hFOB1 1.19 ; American Type Culture Collection, Manassas, VA) were used and cells were cultured containing DMEM and dichloromethane fraction Phlomidis Radix(100 ng/ml, 1 microgram/ml, 10 microgram/ml) at 34degrees C with 5% CO2 in 100% humidity. MTT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examnine the mineralization. Also bone calcification nodules were evaluated. The cellular activity of hFOB1 was increased in 100 ng/ml, 1 microgram/ml, 10 microgram/ml of dichloromethane fraction of Phlomidis Radix and especially significant increation was showed in 100 ng/ml of dichloromethane fraction of Phlomidis Radix at 6days (p<0.05). ALP level of hFOB1 was significantly increased in 100 ng/ml, 1 microgram/ml, 10 microgram/ml of dichloromethane fraction of Phlomidis Radix and especially more increation was showed in 10 microgram/ml of dichloromethane fraction of Phlomidis Radix (p<0.05). Calcification nodules of hFOB1 significantly increased in 10 microgram/ml of dichloromethane fraction of Phlomidis Radix at 21days of incubation (p<0.05). These results indicate that dichloromethane fraction of Phlomidis Radix has excellent effects on mineralization of hFOB1.
Alkaline Phosphatase ; Fractures, Bone ; Hope ; Humans* ; Humidity ; Methylene Chloride* ; Osteoblasts* ; Osteogenesis* ; Osteoporosis ; Regeneration ; Wound Healing

Using the 15 strains of Vibrio eulnificus isolated from the blood and tissue fluid of the patients with primary septicemia, cultural characteristics were studied on various media. The media to be used in culturing Vibrio vulnificus should contain sodium chloride, because it could not survive on the media without NaCl. The organism grew poorly or not at all on the media with eosin-y, methylene blue, crystal violet, or azide, because these substances suppress its growth. Vibrio vulnificus grew well in the enrichment media such as brain heart infusion, tryptic soy broth, and nutrient broth with 0. 5% NaCl.
Brain ; Cultural Characteristics* ; Gentian Violet ; Heart ; Humans ; Methylene Blue ; Sepsis ; Sodium Chloride ; Vibrio vulnificus* ; Vibrio*

BACKGROUND: Workers who are related with paint stripping process of aircraft parts can be exposed methylene chloride, phenol, chromium hexavalent in using paint remover. Especially, methylene chloride is more important hazard because of chemical asphyxia. METHOD: NIOSH(National Institute of Occupational Safety and Health) Manual of Analytical Methods(NMAM) #1005 was used in sampling and analysis of methylene chloride. 4 air bases were surveyed. RESULT: Airborne concentrations of methylene chloride distributed lognomally. In aircraft paint stripping operation of 4 air bases, geometric mean(GM) of methylene chloride concentrations was 30.40+/-3.39 ppm (n=14) in personal samples and GM of area samples was 2.24 ppm (n=2). 5 of 8 samples showed breakthrough phenomenon. Breakthrough rate([back section]/[front section]) significantly correlated with airborne concentrations (p<0.01) and with detected amounts of methylene chloride(p<0.01). CONCLUSION: Airborne concentrations of methylene chloride in paint stripping process exceed the criteria level in many case(43%). Seriesed charcoal tubes must be used in methylene chloride sampling in compliance with NMAM.
Aircraft* ; Asphyxia ; Charcoal ; Chromium ; Compliance ; Humans ; Methylene Chloride* ; Occupational Health ; Paint* ; Phenol