Ultrasonographic localizations were performed by methylene blue injection in 35 cases of nonpalpable breast lesion. The lesions were sized 0.3cm-1.3cm and biopsied just after localization with 100% success rate. All the patients tolerated well the procedure and had not any complications. Ultrasonoguided dye injection is an accurate, comfortable and simple method for prebiopsy localization of nonpalpable breast lesion.
Breast* ; Humans ; Methylene Blue

A complete septate uterus with cervical duplication and a longitudinal vaginal septum is a rare uterine malformation. The dissection of the septum can be difficult because it is difficult for hysteroscopists to find out initial point, direction and final point of a complete septum. This study aimed the introduction of more efficient surgical procedure using a balloon with methylene blue. We have experienced three cases with a complete septate uterus. We performed hysteroscopic dissection of a complete uterine septum using a balloon with methylene blue and obtained good reproductive outcomes. So we report three cases with a brief review of literatures.
Methylene Blue ; Uterus

The purpose of this study is to evaluate the sealing effect of several root-end filling materials using spectrophotometric analysis. 180 single root teeth with one canal were instrumented and canal filled. Root resected and root end preparation was made. Teeth were randomly classified to 5 experimental group(MTA, EBA, IRM, TCP, ZOE) and 1 control group according to root-end filling material MTA group used PRO ROOT MTA, EBA group used Super EBA, TCP group used NEW APATITE LINER TYPE II main component of which is alpha-tricalcium phosphate(TCP). According to manufacture's instruction experimental material was mixed and retrfilled. After 2% methylene blue solution penetration absorbance for each test sample was measured with spectrophotometer (JASCO UV-530, Japan). The mean absorbance of control and experimental group was as follows; MTA : 0.092, IRM : 0.226, Super EBA : 0.255, ZOE : 0.374, Control : 0.425, TCP : 0.501 and the result analyzed by Turkey test at P=0.05 level. Conclusions of this study are as follows; 1. The absorbance increase in follwing sequence MTA, IRM, Super EBA, ZOE, Control, TCP. 2. MTA showed the least leakage but was not significant with IRM or Super EBA and was significant with control or TCP(p<0.05). 3. TCP had the most leakage and was not significant with control group.
Methylene Blue ; Tooth ; Turkey ; Pemetrexed

The purpose of this study was to investigate the color stability of composite resins used widely as esthetic restorative material.Surefil(Dentsply,U.S.A.),Esthet X(Dentsply,U.S.A.),Filtek P60(3M,U.S.A.),Filtek Z250(3M,U.S.A.),Synergy Compact(Coltene,Switzerland),and Synergy Duo(Coltene,Switzerland)were chosen for this study.Thirty six specimens(7mm x2mm) of each composite resin were fabricated and polished after polymerization.Treatment conditions designed for this study were thermocycling,methylene blue staining,and filtered coffee staining. The color changes before and after treatment were measured by colorimeter(colorimeter,Model Tc-6Fx,Tokyo Denshoku Co.)and analyzed. The followings were drawn from this study: 1.The color changes of Filtek Z250,Surefil,Filtek P60 and Esthet X after thermocycling for 3 weeks were greater than those of the others. 2.The color changes of Surefil and Esthet X after methylene blue staining and those of Surefil, Filtek Z250 and Filtek P60 after coffee staining were great. 3.The color changes of Synergy Duo and Synergy Compact after thermocycling and coffee staining were relatively lesser than those of other composite resins.
Coffee ; Composite Resins ; Methylene Blue

The purpose of this study was to investigate the possibility on recycling of IPS Empress ceramic for the wide use of esthetically pleased IPS-Empress ceramic in clinical prosthodontic treatment. The specimens were made of first, second, and third pressed IPS Empress ceramic and were immersed in 0.05% methylene blue solution for 24hours. The amount of color change before and after stainning was measured with colorimeter(Model Tc-6Fx, Denshoku Co., Tokyo, Japan). The change of E in first pressed ceramic was 18.33 and those in second and third pressed ceramics were 19.33 and 19.93 respectively. Although there was statistical difference among them, the differences were minute and acceptable. From the results of this study, the possibility of recycling of IPS Empress ceramic could be suggested.
Ceramics* ; Methylene Blue ; Prosthodontics ; Recycling

This study evaluated the microleakage and interfacial gap between enamel and composite resin under the dry and wet condition of the enamel surface. V shaped class 5 cavities were prepared on the occlusal portion of extracted human molars. Samples were divided into three groups: D group (air dry for 10-15 s), BD group (blot dry with moist cotton pellet), and DR group (air dry for 10-15 s and rewet with Aqua-Prep F for 20 s). Cavities were filled using Aelitefil composite resin after applied One-Step. Microleakage was tested by 2% methylene blue dye solution and the data were statistically analysed by Kruskal-Wallis test and Mann-Whitney test. Also Enamel-resin interface was observed under SEM. Group BD showed statistically lower microleakage than group D (p < 0.05), but there was no statistically significant difference between group BD and DR (p > 0.05). At the enamel-resin interface, group D showed the gap of 2 microm thickness, but group BD and DR showed close adaptation. In conclusion, the use of blot dry and rewetting agent (Aqua-Prep F) resulted in decreased microleakage and improved adhesion between enamel and resin when using One-Step.
Dental Enamel* ; Humans ; Methylene Blue ; Molar

The purpose of this study was to compare the ability of three resin surface sealants to prevent microleakage in Class V composite resin restorations. Forty Class V cavities with the occlusal margin in enamel and gingival margin in dentin were prepared on the buccal surfaces of sound extracted molars, and restored with composite resin. Restorations were randomly assigned into one of four equal groups (n = 10): a control group, without resin sealing, and three experimental groups in which margins were sealed with Fortify Plus, Biscover and Permaseal, respectively. Specimens were thermocycled, immersed in a 2% methylene blue solution for 4 hours, sectioned longitudinally, and observed the leakage at the occlusal and gingival margins. The result was analyzed using Kruskal-Wallis test, Mann-Whitney test and Wilcoxon signed rank test. In conclusion, the ability to reduce microleakage at occlusal margins was similar in all of three sealants. However at gingival margin, it depended on the type of used resin surface sealant. At gingival margin, control and Fortify Plus group showed statistically higher microleakage than PermaSeal group, and Fortify Plus group also showed higher microleakage than BisCover group (p < 0.05).
Dental Enamel ; Dentin ; Methylene Blue ; Molar

Humans ; Methylene Blue ; Melanoma ; Staining and Labeling

PURPOSE: This study examines the change in distance from the corneal limbus to the insertion of the rectus muscles before and after disinsertion and retraction with a pair of fixation forceps during strabismus surgery. METHODS: In 38 strabismus patients, on 30 medial rectus muscles and 38 lateral rectus muscles, before and after disinsertion and retraction with a pair of fixation forceps, the distances from the corneal limbus to the upper, middle and lower parts of the insertion of the medial and lateral rectus muscles marked with methylene blue solution were measured. RESULTS: The distances between the corneal limbus and upper, middle and lower parts of the insertion after the disinsertion were reduced on, average 0.23 mm, 0.28 mm, and 0.18 mm, for the medial rectus muscle, respectively, and 0.21 mm, 0.28 mm, and 0.15 mm, for the lateral rectus muscle, respectively (P<0.05). The percentage of cases in which the advance from the corneal limbus to the insertion was greater than 0.5 mm was 33.3% for the medial rectus muscle, and 21.1% for the lateral rectus muscle. The lateral rectus muscle was disinserted, fixed with a pair of forceps, and subsequently, the distance from the upper, middle and lower parts to the corneal limbus were reduced to 0.36 mm, 0.43 mm, and 0.30 mm, respectively (P<0.05). The percentage of cases that advanced more than 1.0 mm was 13.2 %. The changes in distance from the upper, middle, and lower parts of insertion toward the limbus after disinsertion and retraction were 0.15 mm 0.15 mm, 0.16 mm, respectively (P<0.05). CONCLUSIONS: When performing the recession of the lateral rectus muscle, disinsertion of the rectus muscle, may result in a change of the site of insertion, which in turn might influence the outcome of strabismus surgery.
Humans ; Limbus Corneae ; Methylene Blue ; Muscles* ; Strabismus ; Surgical Instruments

Intraductal papillary tumors of the breast exhibit bloody discharge with often no evidence of a palpable tumor, making discrimination between benign and malignant lesions sometimes difficult for pathologists and surgeons. So the technique used in removing the offending ductal system should be fully diagnostic, adequately therapeutic and cosmetically. Microdochectomy has been accepted treatment for intraductal papilloma of the breast. We performed dye-injection microdochectomy on intraductal papilloma of the breast in 15 patients. This procedure utilizes lacrimal probe and 24-gauge medicut that are gently advanced into the offending duct and methylene blue dye is injected to outline the involved duct for easy dissection through a circumareolar incision. Because we could perform dye-injection microdochectomy safely, rapidly and effectively, we think that this procedure is standard treatment of intraductal papilloma of the breast.
Breast* ; Discrimination (Psychology) ; Humans ; Methylene Blue ; Papilloma, Intraductal*