Poisoned the rats by pesticide wofatox with dose 10mg/kg of their body weight. After 60 minutes, cut off two third of liver by method of Higgins and Anderson. The changes in morphological structures and mitotic activity of liver rats were as follows: Lesions in the liver were markedly manifested, but were not prolong. The mitotic activity of hematocytes decreased. The regenenation of liver weakly manifested
Methyl Parathion ; Rats ; Liver

Study on the changes of ultra and micro-structure of the liver of white rats, which was effected by Wafatox with longed-low dose. Rats with the beginning body mass of 120-150g were everyday oral administrated by oil containing 0.1% of Wafatox with the dose of 0.5mg/kg of their body mass (similarly with 1/70 dose of LD50 for white rats). They were killed by stages of 10, 20, 30, 40 and 50 days from the first oral administration. The results: in clinical, the rat’s intoxicated symptoms were not clear, but the changes in the liver were clearly shown. In the liver, there were some points of necrozism, hepatocytes strongly regressed at the level of ultra and micro-structure. The portals areas and the liver’s tissues were infiltrated by cells like lymphocytes. There were happened stretching veins, stagnating blood and particularly, getting thicker and thicker in the walls of veins of central lobules of the rats’ liver at every stage. Up to the 50th day of intoxication by Wafatox, the quantity of two-nucleus hepatocytes was decreased and mitotic activity of hepatocytes was not increased
Methyl Parathion ; Rats ; Liver

Chromatography, Gas ; methods ; Dimethoate ; blood ; Humans ; Insecticides ; blood ; Methyl Parathion ; blood

The evaluation of the bioavailability of pollutants in soil is crucial to accurately assess the pollution risk, and whole-cell biosensor is one of the important tools for such evaluation. This study aimed to develop a novel whole-cell biosensor for the detection of methyl parathion in soil using. First, a whole-cell biosensor was constructed by the screened methyl parathion hydrolase mpd gene, the existing specific induction element pobR, and the pUC19 plasmid skeleton. Then, the detection method of methyl parathion in soil extracts was established using 96-well microtiter plate as carrier and five whole-cell biosensors as indicator. The method was applied in the detection of methyl parathion in tested and field soil extracts. Taking E. coli DH5α/pMP-AmilCP with the best detection performance as an example, this biosensor had a detection limit of 6.21-6.66 µg/L and a linear range of 10-10 000 µg/L for methyl parathion in four soil extracts. E. coli DH5α/pMP-RFP and E. coli DH5α/pMP-AmilCP methods have good detection performance for the analysis of methyl parathion in soil extract samples. This biosensor method can help to quickly assess the bioavailability of methyl parathion in soil, and thus help to understand the risk of soil pollution caused by organophosphorus pesticide methyl parathion.
Methyl Parathion/analysis* ; Pesticides/analysis* ; Organophosphorus Compounds ; Escherichia coli/genetics* ; Soil ; Farms ; Biosensing Techniques

Animals ; Chromatography, Thin Layer ; Dichlorvos ; blood ; Dimethoate ; blood ; Insecticides ; blood ; Methyl Parathion ; blood ; Mice ; Organothiophosphorus Compounds ; blood