1.Blood oxidant/antioxidant status in overfed aging rats: comparative effects of sunflower and olive-linseed mixture oil rich diets.
Amina AYAD ; Hafida MERZOUK ; Sid Ahmed MERZOUK ; Michel NARCE
Biomedical and Environmental Sciences 2013;26(9):765-768
Aging
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blood
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Animals
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Biomarkers
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blood
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Diet, High-Fat
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Male
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Obesity
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blood
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Oxidative Stress
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drug effects
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Plant Oils
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pharmacology
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Random Allocation
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Rats
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Rats, Wistar
2.Effects of Maternal Linseed Oil Supplementation on Metabolic Parameters in Cafeteria Diet-induced Obese Rats.
Nawel BENAISSA ; Hafida MERZOUK ; Sid Ahmed MERZOUK ; Michel NARCE
Biomedical and Environmental Sciences 2015;28(4):298-302
Because linseed oil may influence maternal and fetal metabolisms, we investigated its role in the modulation of lipid metabolism in cafeteria diet-induced obese rats and their offspring. Female Wistar rats were fed control or cafeteria food, which were either supplemented or not supplemented with linseed oil (5%) for 1 month before and during gestation. At parturition, serum and tissue lipids and enzyme activities were analyzed. Cafeteria diet induced adverse metabolic alterations in both mothers and offspring. Linseed oil improved metabolic status. In conclusion, linseed oil displayed health benefits by modulating tissue enzyme activities in both obese mothers and their newborns.
Animal Feed
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analysis
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Animals
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Diet
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adverse effects
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Dietary Supplements
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analysis
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Female
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Linseed Oil
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administration & dosage
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metabolism
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Lipid Metabolism
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drug effects
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Maternal Nutritional Physiological Phenomena
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drug effects
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Obesity
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drug therapy
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etiology
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metabolism
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Pregnancy
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Prenatal Exposure Delayed Effects
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drug therapy
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etiology
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metabolism
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Random Allocation
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Rats
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Rats, Wistar
3.In vivo anti-hyperglycemic activity of saliva extract from the tropical leech Hirudinaria manillensis.
Abdualkader Abdualrahman MOHAMMED ; Ghawi Abbas MOHAMMAD ; Alaama MOHAMED ; Awang MOHAMED ; Merzouk AHMED
Chinese Journal of Natural Medicines (English Ed.) 2013;11(5):488-493
The anticoagulant effect of leech saliva was traditionally employed in the treatment of diabetes mellitus complications such as peripheral vascular complications. This study was carried out to examine the effect of leech saliva extract (LSE) on blood glucose levels in alloxan-induced diabetic rats. First, LSE was collected from leeches which were fed on a phagostimulatory solution. Second, total protein concentration was estimated using the Bradford assay. Third, diabetic rats were injected subcutaneously (sc) with LSE at doses of 500 and 1 000 μg·kg(-1) body weight (bw). Other diabetic rats were injected sc with insulin at doses of 10 and 20 U·kg(-1) bw. Another group was injected simultaneously with LSE (250 μg·kg(-1) bw) and insulin (10 U·kg(-1) bw). Fasting blood glucose (FBG) concentrations were monitored during a study period of eight hours at regular intervals. Findings showed that both doses of LSE resulted in a significant and gradual decrease in FBG starting from 10%-18% downfall after two hours of injection reaching the maximal reduction activity of 58% after eight hours. Remarkably, LSE was sufficient to bring the rats to a near norm-glycemic state. The high dose of insulin induced a severe hypoglycemic condition after 2-4 h of injection. The lower dose was able to decline FBG for 2-6 h in rats which became diabetic again after 8 h. On the other hand, the concurrent injection of low doses of LSE and insulin produced a hypoglycemic effect with all rats showing normal FBG levels. Taken together, these findings indicated that the subcutaneous injection of LSE of the medicinal Malaysian leech was able to provide better glycemic control compared with insulin. Moreover, the synergism between LSE and insulin suggests that LSE could be utilized as an adjuvant medication in order to reduce insulin dosage or to achieve better control of blood glucose.
Animals
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Biological Factors
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administration & dosage
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Blood Glucose
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metabolism
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Diabetes Mellitus, Experimental
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drug therapy
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metabolism
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Humans
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Hypoglycemic Agents
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administration & dosage
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Leeches
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chemistry
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Male
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Rats
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Rats, Sprague-Dawley
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Saliva
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chemistry