Objective:To explore effects of nurses' cognition of patient safety culture and psychological capital on career growth, so as to provide a basis for promoting nurses' career growth.Methods:This research was a cross-sectional survey. From July to August 2019, a total of 480 nurses from 3 ClassⅢ Grade A hospitals in Tianjin were selected as the research objects by means of convenient sampling. General information questionnaire, Hospital Survey on Patient Safety Culture (HSOPSC) , nurses' Psychological Capital Questionnaire (PCQ) and Career Growth Scale (CGS) were used to investigate the patients. In this study, 480 questionnaires were issued and 465 valid questionnaires were collected, with an effective recovery rate of 97%. Stratified regression analysis was used to analyze the mediating effect of psychological capital, and AMOS was further used to construct the structural equation model for verification.Results:The total mean score of HSOPSC of 465 nurses was (3.91±0.30) , the total mean score of PCQ was (4.66±0.35) and the total mean score of CGS was (3.62±0.41) . Nurses' cognition of patient safety culture was positively correlated with career growth ( r=0.525, P<0.01) . Nurses' cognition of patient safety culture was positively correlated with psychological capital ( r=0.470, P<0.01) . There was a positive correlation between psychological capital and career growth ( r=0.557, P<0.01) . The direct path coefficient of patient safety culture cognition on career growth was 0.33. Psychological capital played a part of the mediating role in the relationship between patient safety cultural cognition and career growth. The mediating effect was 0.30, and the mediating effect accounted for 47.6% of the total effect. Conclusions:The career growth of nurses is at a medium level, and psychological capital plays an intermediary role between the patient's cultural awareness of safety and career growth. Nursing managers should take effective measures to improve nurses' cognition of patient safety culture, at the same time improve nurses' psychological capital level, so as to promote nurses' career growth.

Objective: To observe the effects of He-Ne laser on the expression of extracellular matrix and its regulatory factors in degenerated temporomandibular joint(TMJ) of rabbits. Methods: 40 New Zealand adult white rabbits were randomly divided into normal group,sham model group,TMJOA model group and laser treatment group(n = 10),the rats in treatment group were treated by He-Ne laser irradination at acupoints. The rats in each group were divided into 1 d and 11 d groups(n = 5). The animals were respectively killed 1 d and 11 d after operation and HE staining was used to observe the histomorpholy. The protein expression of extracellular matrix and its regulatory factors were examined by Western blot. Results: After He-Ne laser treatment,the fiber layer of condylar cartilage was slightly loose,part of the fiber was newly produced. The level of Col-2,PRG-4,TIMP-1,BMP-2 was up-regulated and the of MMP-13 was down-regulated in the 11 d treatment group. Conclusion: He-Ne laser irradiation on acupoints may up-regulate the expression of extracellular matrix (Col-2 and PRG-4) and its regulatory factors (TIMP-1 and BMP-2),down-regulate the expression of MMP-13.

Objective? To evaluate the rehabilitation effect of phase I cardiopulmonary rehabilitation on patients with coronary artery bypass grafting(CABG). Methods? Chinese and English databases were searched via computer including PubMed, Embase, Cochrane Library, Web of Science, CNKI, Wanfang, VIP, with the time set as the database establishment date to 31st January of 2019, to obtain randomized controlled trials of phase I cardiopulmonary rehabilitation in CABG. Meta-analysis of the included studies was performed by RevMan 5.3 software. Results? A total of 8 studies were included, according to the Meta analysis, 6 min walking distance increased in the phase I cardiopulmonary rehabilitation patients group(MD=56.46,95%CI 32.82-80.09,P＜ 0.001), the percentage of forced vital capacity(FVC) in expected value(1%) increased (MD=7.30,95%CI 2.59-12.00,P=0.002), the percentage of forced expiratory volume in first second(FEV1) in the expected value(1%) increased (MD=7.93,95%CI 3.13-12.72,P=0.001), hospital stay decreased(MD=-5.35,95%CI -9.67--1.03,P=0.002), incidence of postoperative arrhythmia decreased(MD=0.42, 95%CI -0.21-0.85),P=0.002), but the improvement effect of quality of life was not significan(t P＞ 0.05). Conclusions? Phase I cardiopulmonary rehabilitation can improve the activity endurance, pulmonary function, shorten the hospitalization time and reduce the incidence of postoperative arrhythmia, but it can not significantly improve the quality of life. Due to the small number of included studies and the heterogeneity of some indicators, the results of this study still need to be verified by multicenter, large sample and high-quality randomized controlled trials.

Objective:To understand the vancomycin dose, therapeutic drug monitoring(TDM) situation and therapeutic effect of children after liver transplantation.Methods:A retrospective analysis of the data of 98 children who received intravenous vancomycin treatment after liver transplantation were conducted in the Department of Critical Care Medicine of Beijing Friendship Hospital from January 2017 to June 2019, including demographic data, vancomycin dose, serum trough concentration, drug-related adverse reactions and clinical outcome data.Results:A total of 98 children received intravenous vancomycin treatment and at least one steady-state TDM blood sample was collected.Among them, 53 cases (54.1%) were male, and the median age was 9 months(5 months to 14 years old). The median first daily dose of vancomycin treatment was 50(30-60)mg/(kg·d), and the median duration of treatment was 14(3-54)days.Only 27.5%(27/98)of the children′s initial trough concentration reached the target concentration (10-20 mg/L), while 26 cases(26.5%) did not reach the target after adjusting the treatment.Six children(6.1%)had renal toxicity caused by vancomycin, and two children had skin rash.The effective treatment rate accounted for 51.7%(15/29). The initial trough concentrations of vancomycin in the effective and ineffective groups were(5.92±3.82)mg/L and(10.43±5.37)mg/L, respectively.The difference was statistically significant ( P=0.041). Conclusion:The rate of intravenous vancomycin in children after liver transplantation is low, and the dose needs to be adjusted individually.

AIM: To observe the effects of rapamycin pregnancy intervention on cognitive function of autism model in rat offspring. METHODS: Fourteen pregnant rats were randomly divided into normal group (n = 3), model group (n = 4), rapamycin (RAPA) control group (n =3) and intervention group (n = 4). The model group and intervention group were i.p. injected with sodium valproate 600 mg/kg at embryonic day (E) 12.5 to establish autism model in rat offspring. RAPA control group and intervention group were i.g. given RAPA 4 mg/kg every day from the 13th day of gestation until the offspring rats were weaned at 23 days. After the birth of the above four groups of pregnant rats, 15, 27, 21 and 26 offspring male rats were selected to conduct behavioral tests to identify the model. Then, paw withdrawal mechanical threshold (PWMT), tail flick latency (TFL) evoked under different light intensity and learning and memory function of offspring rats were further detected. RESULTS: Rat offspring in the model group had lower growth and development indexes and exploratory behavior ability, but stronger repetitive stereotyped behavior compared with the normal group (P < 0.05), while the indexes between the intervention group and model group were reversed (P < 0.05). The model group had higher PWMT than normal group (P < 0.01) and the PWMT of intervention group was lower than that of model group (P < 0.01). The TFLs of rats in 4 groups showed a timed dose-response relationship (TDRR, P < 0.01), that is, TFLs were shortened with the increase of light intensity. The TDRR curve of model group shifted to right compared with normal group (P < 0.01) and intervention group shifted to left compared with model group (P < 0.01). At the light intensity of Focus 34, 51 and 76, the TFLs of model group were longer than those of normal group (P < 0.01) and intervention group had shorter TFLs compared with model group (P<0.01). In spatial probing trial of Morris water maze test, the platform crossover number in model group was less than that in normal group (P<0.01) and that in intervention group was more than model group (P < 0.05). CONCLUSION: RAPA intervention during pregnancy may alleviate behavior disorder, pain tolerance and memory function of autism model in rat offspring to some extent.

OBJECTIVE: To explore the regulatory mechanism of human hepatocyte apoptosis induced by lysosomal membrane protein Sidt2 knockout. METHODS: The Sidt2 knockout (Sidt2^-/-) cell model was constructed in human hepatocyte HL7702 cells using Crispr-Cas9 technology.The protein levels of Sidt2 and key autophagy proteins LC3-II/I and P62 in the cell model were detected using Western blotting, and the formation of autophagosomes was observed with MDC staining.EdU incorporation assay and flow cytometry were performed to observe the effect of Sidt2 knockout on cell proliferation and apoptosis.The effect of chloroquine at the saturating concentration on autophagic flux, proliferation and apoptosis of Sidt2 knockout cells were observed. RESULTS: Sidt2^-/- HL7702 cells were successfully constructed.Sidt2 knockout significantly inhibited the proliferation and increased apoptosis of the cells, causing also increased protein expressions of LC3-II/I and P62(P < 0.05) and increased number of autophagosomes.Autophagy of the cells reached a saturated state following treatment with 50 μmol/L chloroquine, and at this concentration, chloroquine significantly increased the expressions of LC3B and P62 in Sidt2^-/- HL7702 cells. CONCLUSION Sidt2 gene knockout causes dysregulation of the autophagy pathway and induces apoptosis of HL7702 cells, and the latter effect is not mediated by inhibiting the autophagy-lysosomal pathway.
Humans ; Lysosome-Associated Membrane Glycoproteins/metabolism* ; Autophagy ; Apoptosis ; Hepatocytes ; Lysosomes/metabolism* ; Chloroquine/pharmacology* ; Nucleotide Transport Proteins/metabolism*