1.Study on influence of glucoside Tripterygium total tablets on metabolism in rats by NMR metabonomic technique.
Mengxiang SU ; Xuan GAO ; Min SONG ; Taijun HANG ; Wenbin SHEN ; Zhe SONG
China Journal of Chinese Materia Medica 2011;36(11):1449-1453
OBJECTIVETo investigate the toxic effects of Glucoside Tripterygium total on rats with nuclear magnetic resonance (NMR)-based metabonomic method.
METHODThe influence of intragastric administration of Glucoside Tripterygium total suspension at two different doses on endogenetic metabolites in normal rat urine was determined with bio-NMR method then analyzed by pattern recognition technique and partial least-squares discriminant analysis (PLS-DA). Histopathological analysis was carried out.
RESULTEscalations of concentrations of urinary taurine, TMAO and glucose as well as reductions of concentrations of urinary citrate and 2-oxoglutarate were found by analysis of the 1H-NMR spectra, which was coincident with the result of histopathological analysis. The result of pathological examination indicated that pathologic change was not observed in nephridial tissue, but there were obvious changes in hepatic tissue.
CONCLUSIONThe urinary metabomic spectra were closely associated with the hepatic toxicity, which manifested the mitochondrial dysfunctions, the abnormal energy metabolism in TCA cycle as well as the abnormal glucose metabolism.
Animals ; Citric Acid ; urine ; Enteral Nutrition ; Glucose ; metabolism ; Glucosides ; administration & dosage ; Ketoglutaric Acids ; urine ; Least-Squares Analysis ; Liver ; drug effects ; metabolism ; pathology ; Magnetic Resonance Spectroscopy ; methods ; Metabolomics ; Methylamines ; urine ; Plant Extracts ; administration & dosage ; Rats ; Tablets ; administration & dosage ; Taurine ; urine ; Tripterygium ; chemistry
2.Progresses in the role of HMGB1/RAGE axis in tumor inflammation and the research of its targeting drug papaverine
Anqi FAN ; Xuhuan TANG ; Fang ZHENG ; Chenchen WANG ; Quan GONG ; Lian LIU ; Mengxiang GAO
Immunological Journal 2023;39(10):916-920
HMGB1's role in tumors is complex and diverse,and it exerts its biological function by combining with different receptors.One of the receptors is called RAGE,which is localized to the cell membrane and binds to HMGB1 released outside the cell.The HMGB1/RAGE axis promotes tumor development,moreover,tumor development and its drug resistance are closely related to inflammation.This article mainly reviews the molecular mechanism of HMGB1/RAGE axis in pro-inflammatory and protumor effects in pancreatic,colorectal and liver cancers.We also summarize the research progress of papaverine and its derivatives for the treatment of HMGB1/RAGE axis in tumor inflammation,with aims of providing new ideas for exploring the molecular mechanism of action in tumor inflammation,and providing a new theoretical basis for the research of HMGB1/RAGE axis therapeutics.
3. Isolation and identification of Prevotella nigrescens in patients with chronic periodontitis and analysis of its tumorigenic role in esophageal squamous carcinogenesis
Qiwei LIU ; Yelin JIAO ; Haojie RUAN ; Pan CHEN ; Ke LIU ; Mengxiang LI ; Bianli GU ; Shegan GAO ; Yijun QI
Chinese Journal of Microbiology and Immunology 2020;40(1):49-54
Objective:
To isolate and identify
4.Lysosomal membrane protein Sidt2 knockout induces apoptosis of human hepatocytes in vitro independent of the autophagy-lysosomal pathway.
Jiating XU ; Mengya GENG ; Haijun LIU ; Wenjun PEI ; Jing GU ; Mengxiang QI ; Yao ZHANG ; Kun LÜ ; Yingying SONG ; Miaomiao LIU ; Xin HU ; Cui YU ; Chunling HE ; Lizhuo WANG ; Jialin GAO
Journal of Southern Medical University 2023;43(4):637-643
OBJECTIVE:
To explore the regulatory mechanism of human hepatocyte apoptosis induced by lysosomal membrane protein Sidt2 knockout.
METHODS:
The Sidt2 knockout (Sidt2-/-) cell model was constructed in human hepatocyte HL7702 cells using Crispr-Cas9 technology.The protein levels of Sidt2 and key autophagy proteins LC3-II/I and P62 in the cell model were detected using Western blotting, and the formation of autophagosomes was observed with MDC staining.EdU incorporation assay and flow cytometry were performed to observe the effect of Sidt2 knockout on cell proliferation and apoptosis.The effect of chloroquine at the saturating concentration on autophagic flux, proliferation and apoptosis of Sidt2 knockout cells were observed.
RESULTS:
Sidt2-/- HL7702 cells were successfully constructed.Sidt2 knockout significantly inhibited the proliferation and increased apoptosis of the cells, causing also increased protein expressions of LC3-II/I and P62(P < 0.05) and increased number of autophagosomes.Autophagy of the cells reached a saturated state following treatment with 50 μmol/L chloroquine, and at this concentration, chloroquine significantly increased the expressions of LC3B and P62 in Sidt2-/- HL7702 cells.
CONCLUSION
Sidt2 gene knockout causes dysregulation of the autophagy pathway and induces apoptosis of HL7702 cells, and the latter effect is not mediated by inhibiting the autophagy-lysosomal pathway.
Humans
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Lysosome-Associated Membrane Glycoproteins/metabolism*
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Autophagy
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Apoptosis
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Hepatocytes
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Lysosomes/metabolism*
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Chloroquine/pharmacology*
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Nucleotide Transport Proteins/metabolism*