OBJECTIVETo study water-soluble chemical constituents from the leaves of Elaeagnus pungens.

METHODChemical constituents of E. pungens leaves were separated by a combination of macroporous resin column chromatography, reverse phase silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties using the spectral method.

RESULTThe two compounds were separated from E. pungens leaves and identified as kaempferol 3-O-P-D-glucopyranosyl- (1-->3)-alpha-L-rhamn-opyranosyl-(1-->6) -/3-D-galactopyranoside (1), kaempferol 3-O-P-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (2).

CONCLUSIONCompound 2 separated from E. pungens leaves was a new compound.

Elaeagnaceae ; chemistry ; Glucosides ; chemistry ; Plant Leaves ; chemistry ; Solubility ; Water ; chemistry