Objective To investigate the feature of fracture in elderly patients in Beijing Jishuitan Hospital from 2009 to 2016.Methods The data of elderly patients with fracture visiting the department of trauma emergency in Beijing Jishuitan Hospital from 2009 to 2016 were retrospectively reviewed.The data of patient number,age,gender,seasonal and circadian variation of visit and fracture site were collected and analyzed.Results Among all the 42 988 elderly patients with fractures visiting department of trauma emergency from 2009 to 2016,there were more female patients than male patients (P ＜ 0.01).In both male and female patient groups,there was a trend of increase in number of patients year by year (P ＜ 0.01).Elderly patients in the groups of 60-69 years,70-79 years and 80-89 years demonstrated a trend of increasing number from year to year notably in the 60-69 years group (P ＜ 0.01) but the total number of patients decreased as age increased.As for seasonal variation,autumn had the highest number of cases and spring the lowest (P ＜ 0.01).Most elderly patients visited emergency during 8:00-16:00 period (P ＜ 0.01).The commonest sites of fractures were at radius and femur.Conclusions Fracture of elderly patients has its unique feature in gender,age,seasonal and circadian variation of visit and fracture sites.The community should do a better planning for prevention and management of elderly patients with fractures according to their specially clinical features.

A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine) oxidation deposition. It was shown that gold nano-particles(colloid Au) could be used as a platform to immobilize antibodies by adsorption. By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase(HRP) as the second antibody and catalytic amplification by biotin-tyramine, the immunosensor′s) catalytic ability to hydrogen peroxide increased nearly 20 times), the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 μg/L-22 mg/L, and the detection limit was 0.1 μg/L), the regression equation could be expressed as Δi_p(μA) =2.8859c(mg/L)+17.152 with a correlation) coefficient of 0.9872. The immunosensor can be used to quantitatively determine hIgG in the sample) of human serum).

Objective To investigate the expression of matrixmetalloprotein -9(MMP-9)and E-cad-herin in triple negative breast cancer ( TNBC) and its relationship with the clinicopathologic features of TNBC .To analyze the function of MMP -9 and E-cadherin in TNBC .Methods Immunohistochemical method was used to detect the expression of MMP -9 and E-cadherin in 127 cases of TNBC.Results The MMP-9 positive rate was 53.54%and E-cadherin positive grade was 32.28%in TNBC.Expression of MMP-9 was correlated with tumor size(P=0.007),histological grade(P=0.006),TNM stage(P=0.003),lymph node metastasis(P=0.000)and lymph duct invasive(P=0.000).Expression of E-cadherin was correlated with lymph node metasta-sis(P=0.016)and lymph duct invasive(P=0.015).However,they were not correlated with other factors .Con-clusion The expression of MMP -9 and E -cadherin was correlated with invasion and metastasis in TNBC , which could be an important research topic in the future studies .

Morinda genus of Rubiaceae has been widely used in medicine at home and abroad. Many parts of Morinda tree are utilized in research, mainly including roots, stems, leaves, branches and seeds. Through the research of online databases, the chemical components and biological activities of the iridoids in Morinda genus were summarized in this paper. Up to now, more than 50 kinds of iridoids have been identified. In addition, more and more studies proved that Morinda iridoids might benefit human via such anti-inflammatory, antinociceptive, anti-oxidation, anti-tumor and bone protection. The theoretical basis was provided for the further development and utilization of the iridoids in Morinda genus.

ObjectiveTo investigate the key compounds affecting the irritation and to clarify the effect of heating and the addition of ginger juice as the auxiliary material during the processing on the irritation of Magnoliae Officinalis Cortex（MOC） by comparing the irritation and composition of volatile oil in MOC and its different processed products. MethodVolatile oil in raw products， water-processed products， ginger-dried products， ginger-fried products（the amounts of ginger were 10%， 50%， respectively） of MOC were extracted by steam distillation and subjected to rabbit eye irritation experiment， and the volatile components of each sample were detected by gas chromatography-mass spectrometry（GC-MS）. Principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA） were used to analyze the data of each sample by SIMCA 14.1. The relative contents of different processed products were compared two by two with those of and raw products or ginger-fried products， and the markers that might be related to the irritation were sorted out according to the principles of variable importance in the projection（VIP） value >1 and P<0.05， and the factors influencing the differences in irritation were analyzed. ResultCompared with the blank group， the administration groups all had irritation to the eyes of rabbits， and the degree of irritation was in the order of raw products>water-processed products>ginger-dried products>ginger-fried products（10%）>ginger-fried products（50%）. The results of PCA and OPLS-DA showed that there were differences in the volatile oil from raw products and different processed products. According to VIP value>1 and P<0.05， and combined with the results of eye irritation experiment， ten volatile compounds related to irritation changes were screened out. Among them， cis-cinnamaldehyde was only detected in raw products， the relative contents of β-caryophyllene， （+）-delta-cadinene， α-humulene， γ-muurolene， （-）-isoledene and citral all increased to different degrees， the contents of p-cymene， 1（10）-4-cadinadien-15-ol and β-eudesmol all decreased to different degrees. ConclusionThe irritation of MOC is reduced after heating and processing with ginger juice， and the synergistic effect of both is more effective for reducing irritation. Among the differential markers associated with changes in irritation， the increase in the relative content of citral is closely related to the addition of ginger juice， while the decrease in the relative contents of cis-cinnamaldehyde， p-cymene， 1（10）-4-cadinadien-15-ol is related to heating， and the changes of other components may be related to the synergistic effect of heating and ginger juice.