Objective To investigate the sleep problems and related affecting factors of primary or possibly symptomatic school-aged children with epilepsy.Methods 54 epilepsy children(7 to 12 years old) and the general 54 healthy children whose gender and age were strictly matched with epilepsy group were investigated by using the chinese version of CSHQ.Results ① The total CSHQ score and 6 subscale scores (bedtime resistance, sleep onset delay,sleep anxiety, night wakings, parasomnias, and daytime sleepiness)were significantly higher in the epilepsy group(P<0.05).② In the epilepsy group, different age groups had statistical difference in the total CSHQ score and 4 subscale scores(sleep resistance, sleep onset delay, sleep duration and sleep anxiety) (P<0.05).The seizure controlled group and the uncontrolled group had statistical difference in the total CSHQ score and all subscale scores, except sleep onset delay(P<0.05).There were statistically significant differences in the total CSHQ score and all subscale scores, except sleep-disordered breathing in different seizure frequency groups(P<0.05).Different seizure type groups had statistical difference in the total CSHQ score and 4 subscale scores(sleep resistance, sleep duration, sleep anxiety and daytime sleepiness)(P<0.05).Different seizure time groups had statistical difference in the total CSHQ score and 5 subscale scores(sleep resistance, sleep onset delay, sleep duration, sleep anxiety and night waking)(P<0.05).Monotherapy group and polytherapy group had statistical difference in the total CSHQ score and 4 subscale scores(sleep onset delay, night waking, parasomnias and daytime sleepiness) (P<0.05).③ Multiple linear regression analysis showed that the main influencing factors of the total CSHQ score and some subscale scores were gender, age, control of seizure, frequency of seizure, type of seizure, time of seizure and the number of drug taking.Conclusion Children with epilepsy compared with healthy children are more likely to have sleep problems, and the occurrence of sleep problems may be related to many factors.

The paper retrieved the literature information on the artificial bone and composite artificial bone in CNKI, Wanfang data and foreign databases such as Pubmed and SCI-E from 2009 to 2016, and summarized the characteristics and deficiency of all kinds of artificial bone materials.On this basis, it briefly described the functional application and the principles of the composite artificial bone repair materials, and introduced the application of tissue engineering and 3D printing technology in the field, which could provide reference for the exploration of new types of composite artificial bone repair materials.

Humans ; Intestines ; microbiology ; Liver Diseases ; Microbiota

The problems in resources support in Library of Chinese Academy of Medical Sciences were analyzed according to the investigation on the utilization of and demand for information resources in 23 scientific researchers and 37 students in Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.Certain suggestions were put forward for the solution of these problems in terms of adjustment and optimization of subject resources, in-formation organization and revealing, exchange and communication between users, recommendation and training of subject resources .

The brain-gut axis is an important pathway for the interaction between the central nervous system and the gastrointestinal tract. Ischemic stroke can promote the imbalance and displacement of intestinal flora, and the intestinal flora and its metabolites in turn can affect the occurrence, development and outcome of ischemic stroke. This article reviews the related literature on ischemic stroke and intestinal flora, in order to review the relationship between the two and related mechanisms, and to prospect the stroke treatment of targeting intestinal flora.

Heart rate variability (HRV) is a reliable, innocuous and sensitive new index to measure cardiac autonomic nervous function. Anxiety disorder is often accompanied by autonomic nerve dysfunction and its main sign is abnormal HRV. Acupuncture can affect HRV indices, correct abnormal HRV and improve cardiac autonomic nerve dysfunction to relieve anxiety. This article sorts out and analyzes recent years’ studies on abnormal HRV in anxiety disorder, the effect of acupuncture on HRV and acupuncture regulation of abnormal HRV in anxiety disorder to provide a therapeutic basis for clinical acupuncture intervention in abnormal HRV in anxiety disorder.

Objective To observe the effects of functional ambulation training in a realistic environment based on the activities of daily living among stroke patients with hemiplegia.Methods Thirty-two stroke survivors with hemiplegia were randomly divided into an experimental group and a control group,each of 16.Both groups were given routine rehabilitation training,while the experimental group was additionally given functional ambulation training based on the activities of daily living in a realistic environment for 60 min per day,five days a week for six weeks.Both groups were evaluated using the mini-mental state examination (MMSE),the Holden walking functional class assessment (HWFCA),the Berg balance scale (BBS),functional gait assessment (FGA),the 5 times sit to stand test (FTSST),the timed up and go test (TUGT),and rated using the specific activity balance confidence scale (ABC) and the modified Barthel index (MBI).Results Before the training,no significant differences between the two groups were found in terms of any of the measurements.After the six weeks of training,significant improvement was observed in all of the outcome measures except the MMSE and the HWFCA,with the experimental group scoring significantly better,on average,than the control group.Conclusion When combined with routine rehabilitation training,functional ambulation training based on the activities of daily living in a realistic environment can significantly enhance the functional gait,balance and postural control of stroke survivors.This should facilitate their activities in daily life and improve their confidence in maintaining their balance.

Objective To establish an analytical method for assessing acetylcysteine in human plasma and study the relative bioavailability and bioequivalence of acetylcysteine granules in Chinese healthy volunteers. Methods In the randomized crossover study, 24 healthy male volunteers received a single oral dose of 0. 6 g test acetylcysteine granules, reference acetylcysteine granules or no medication. The plasma concentration of acetylcysteine was determined by LC-MS/MS. Pharmacokinetic parameters were calculated and bioequivalence of two preparations were evaluated by DAS3. 0 software. Results The main pharmacokinetic parameters of the test and reference preparations were as follows:AUC0→t was (8 547. 64± 2 860.04) and (8 783.07±4 042. 10) μg·h·L-1, respectively; AUC0→∞ was (9 481. 64±3 444. 76) and (9 540. 51± 4 239. 30) μg·h·L-1, respectively;Cmax was (1 994. 39±726. 42) and (2 090. 27±885. 46) μg·L-1, respectively;tmax was (1.18±0. 60) and (1. 13±0. 53) h, respectively; t1/2 was (8. 60±3. 76) and (7. 75±5. 01) h, respectively. The relative bioavailability F0→t and F0→∞ was ( 107. 0 ± 43. 3 )% and ( 106. 5 ± 40. 1 )%, respectively. Conclusion The results of statistical analysis indicate that the test and reference formulations are bioequivalent.

OBJECTIVE:To optimize the formulation of Methotrexate thermosensitive hydrogels,and to study the in vitro re-lease property of it. METHODS:Taking PLGA-PEG-PLGA as matrix and mannitol as viscosity modifiers,Methotrexate thermosen-sitive hydrogels were prepared. Using the absolute value of the deviation of gelation temperature between 35 ℃,its formulation was optimized by orthogonal test using the concentrations of PLGA-PEG-PLGA,methotrexate and mannitol as factors. The dialysis bag method was used to investigate accumulative release rate of Methotrexate thermosensitive hydrogels and methotrexate solution within 336 h in vitro. RESULTS:The optimal formulation was as PLGA-PEG-PLGA of 20%,methotrexate of 0.10% and mannitol of 0.10%;gelation temperature were 35.1,35.0,35.0℃. The average drug-loading rate was more than 90%. 12 d accumulative re-lease rate was more than 90%(r=3). Methotrexate solution has been substantially completely relased within 240 h. CONCLU-SIONS:Methotrexate thermosensitive hydrogels with sustained-release are prepared successfully,and the preparation technology is simple and practical.

Objective To prepare triamcinolone acetonide acetate(TAA)thermosensitive hydrogel for intra-articular injection,and to investigate its release in vitro. Methods TAA suspending thermosensitive hydrogel was prepared by using poly lactic-co-glycolic acid(PLGA)-polyethylene glycol(PEG)-PLGA as gel matrices,xanthan gum as suspending agent and NaCl as flocculant. It was evaluated preliminarily by determining its contents and its in vitro release. Results The best compositions for preparation of TAA suspending thermosensitive hydrogel were 25% PLGA-PEG-PLGA,0.05% xanthan gum, 0.9% NaCl and 4 mg·mL-1 TAA.The gelation temperature was 35.3 ℃ .The average recovery was(98.98±0.31)%. By the method of membraneless dissolution,the accumulative drug release was up to 83. 31% at 16 days. The drug release followed Ritger-Peppas methematical model. Conclusion The TAA thermosensitive hydrogel with obviously sustained release is expected to become a new drug delivery system for intra-articular injection.