Objective To discuss effect of different temperature scrubbing solution on red blood cell morphological structure. Methods Selected 60 cases with autologous blood transfusion were divided into 4℃ group, 24℃ group and 37℃ group. pH, Na+,K+,Hb, Hct were detected before being scrubbed and after being scrubbed. Red blood cell struc-ture after being scrubbed were observed. Results Levels of Hb, Hct, Na+ of three groups after being scrubbed were higher than those before being scrubbed(P<0.01). And group 4℃was higher than group 24℃and group 37℃(P<0.05). Level of K+of three groups decreased after being scrubbed (P<0.01). After being scrubbed, spines shape of red blood cells increased with extension of time (P<0.01). With increasing of temperature, spines shape of red blood cells in-creased (P<0.01). Conclusion In the process of autologous blood doping, scrubbing solution of 4℃for red blood cell morphology keeping is better than 24℃and 37℃.

Objective To evaluate the efficacy of transversus abdominis plane (TAP) block with dexmedetomidine mixed with ropivacaine for postoperative analgesia in pediatric patients.Methods Forty American Society of Anesthesiologists physical status Ⅰ or Ⅱ patients of both sexes,aged 1-4 yr,weighing 8-22 kg,scheduled for elective unilateral herniorrhaphy,were assigned into 2 groups (n=20 each) using a random number table method:ropivacaine group (group R) and dexmedetomidine mixed with ropivacaine group (group DR).TAP block was performed at the end of operation under ultrasound guidance,0.125％ ropivacaine 1 ml/kg was injected in group R and 0.075％ ropivacaine plus 1 μg/kg dexmedetomidine was injected in group DR.A dose of morphine 0.05 mg/kg was intravenously injected when Face Legs Activity Cry Consolability score ≥4.The duration of postoperative analgesia and consumption of morphine within 24 h after operation were recorded.The development of nausea and vomiting,bradycardia,hypotension,respiratory depression,over-sedation and TAP block-related complications (hematoma or infection at puncture site,peritoneal puncture,local anesthetic toxicity) within 24 h after operation were recorded.Results Compared with group R,the consumption of postoperative morphine within 24 h after operation was significantly reduced,and the duration of postoperative analgesia was prolonged in group DR (P ＜0.05).No hypotension,over-sedation or TAP block-related complications were found in two groups.There was no significant difference in the incidence of nausea and vomiting,respiratory depression or bradycardia after operation between two groups (P＞0.05).Conclusion Dexmedetomidine combined with ropivacaine provides better postoperative analgesic efficacy than ropivacaine alone when used for TAP block in pediatric patients.