Group-A rotaviruses are recognized as the most common cause of acute diarrhea. Phylogenetic analyses of the VP7 genes of rotaviruses circulating in Nanjing (China) could aid in the development of rotavirus vaccines. A total of 908 stool specimens were collected from patients suffering from acute diarrhea in Nanjing between October 2012 and December 2013, and were tested further for rotaviruses. Fifty rotavirus isolates selected randomly were typed by reverse transcription-polymerase chain reaction using serotype-specific primers for G genotyping. VP7 genes of 19 G9 strains were sequenced for further genetic characterization. Among the 908 stool specimens examined during the surveillance period, 103 (11.34%) were rotavirus-positive. G9 was the most predominant genotype (78.0%), followed by G2, G1 and G3. Sequence and phylogenetic analyses of the VP7 genes of serotype G9 rotaviruses revealed these strains to comprise two lineages (G9-VI, G9-III) and to be dominated by the G9-VI lineage (which belonged to a unique subcluster of Japanese and Chinese G9 strains). Amino-acid sequences of the four antigenic regions (A, B, C or F) were variant among a portion of strains, which may have contributed to the prevalence of G9 rotaviruses in this area.
Adult ; Amino Acid Sequence ; Antigens, Viral ; chemistry ; genetics ; Capsid Proteins ; chemistry ; genetics ; China ; Evolution, Molecular ; Humans ; Infant ; Molecular Sequence Data ; Mutation ; Phylogeny ; Rotavirus ; genetics ; immunology ; physiology ; Serogroup

Objective:To investigate the current risk awareness of medical online live webcasts among physicians in public hospital, so as to provide reference in promoting the peaceful development of medical online live webcast.Methods:From March 15th to 31st, 2022, the research team used the convenience sampling method to conduct a questionnaire survey on the risk awareness of medical online live webcast among doctors in public hospitals with the help of the " Physician Daily" Internet platform. All data were analyzed descriptively, and the comparison between groups was conducted by Chi square test.Results:1 015 questionnaires were recovered, of which 828 were valid, with an effective recovery rate of 81.6%. 741 doctors (89.5%) knew about the medical webcast, and 752 doctors (90.8%) thought that the medical webcast was risky. More than 50.0% of doctors believed that misleading the public, disclosing patients′ privacy and ideological risk were the risk points of live webcast; Health science popularization, doctor-patient humanistic activities and free medical activities were suitable for live webcast; Live webcast with goods and medical diagnosis were inappropriate for live webcast. There were significant differences in the cognition of doctors with different professional titles on the ideological risk, disclosure of patient privacy, health science popularization, medical and patient humanistic activities, and live webcasting with goods ( P < 0.05). 219 doctors participated in the medical network live webcast, of which 148 (67.6%) reported live webcast. Conclusions:Physicians in public hospitals are generally aware of medical webcasting and believe that there are risks in such behavior. But physicians with different professional titles have different cognitions on the risk points, suitable content, inappropriate behavior, and live webcastreporting of medical webcasting.

Objective:To analyze the operation status of official voice in Beijing municipal hospitals and explore the countermeasures to optimize the operation of official short video tiktok.Methods:The 21 Beijing municipal tertiary public hospitals were selected as the research objects. The 17 tiktok numbers which had been approved by the hospital as of December 31, 2020 were included. The data of basic accounts, fans, points, themes, contents, and number of periodicity were analyzed. The theme and the number of praise of the short video tiktok that broke through 10 thousand times were analyzed. The current situation and problems of official voice in Beijing municipal hospitals were analyzed.Results:The 17 official tiktok accounts of the hospital issued 2 059 pieces of tiktok, 1 737.5 thousand fans, and 9 231.8 thousand times of points, the average number of entries was 4.5 thousand times. The official tiktok construction of Beijing municipal hospitals was still in its infancy. The problems included: the weakening of IP image and the lack of system design, lack of content productivity and interaction, unclear positioning and lack of operation strategy.Conclusions:The optimization of hospital official tiktok operation needs to properly handle the relationship between science and interest, systematicness and efficiency. We should clarify the account positioning, improve productivity, strengthen interaction, enhance linkage, and do a good job in supervision.