1.Appraisal of Guidelines for the Management of Blood Pressure in Patients with Diabetes Mellitus: The Consensuses, Controversies and Gaps
Menghui LIU ; Shaozhao ZHANG ; Xiaohong CHEN ; Yue GUO ; Xiangbin ZHONG ; Zhenyu XIONG ; Yifen LIN ; Huimin ZHOU ; Yiquan HUANG ; Zhengzhipeng ZHANG ; Lichun WANG ; Xiaodong ZHUANG ; Xinxue LIAO
Diabetes & Metabolism Journal 2021;45(5):753-764
Background:
Currently available guidelines contain conflicting recommendations on the management of blood pressure (BP) in patients with diabetes mellitus (DM). Therefore, it is necessary to appraise the guidelines and summarize the agreements and differences among recommendations.
Methods:
Four databases and the websites of guideline organizations were searched for guidelines regarding BP targets and thresholds for pharmacologic therapy in DM patients, and the included guidelines were appraised with the Appraisal of Guidelines for Research and Evaluation (AGREE) II instrument.
Results:
In 6,498 records identified, 20 guidelines met our inclusion criteria with 64.0% AGREE II scores (interquartile range, 48.5% to 72.0%). The scores of the European and American guidelines were superior to those of the Asian guidelines (both adjusted P<0.001). Most of the guidelines advocated systolic BP targets <130 mm Hg (12 guidelines, 60%) and diastolic BP targets <80 mm Hg (14 guidelines, 70%) in DM patients. Approximately half of the guidelines supported systolic BP thresholds >140 mm Hg (10 guidelines, 50%) and diastolic BP thresholds >90 mm Hg (nine guidelines, 45%). The tiny minority of the guidelines provided the relevant recommendations regarding the lower limit of official BP targets and the ambulatory BP monitoring (ABPM)/home BP monitoring (HBPM) targets and thresholds in DM patients.
Conclusion
The lower official BP targets (<130/80 mm Hg) in patients with DM are advocated by most of the guidelines, but they contain conflicting recommendations on the official BP thresholds. Moreover, the gaps regarding the lower limit of official BP targets and the ABPM/HBPM targets and thresholds need to be considered by future study.
2.Appraisal of Guidelines for the Management of Blood Pressure in Patients with Diabetes Mellitus: The Consensuses, Controversies and Gaps
Menghui LIU ; Shaozhao ZHANG ; Xiaohong CHEN ; Yue GUO ; Xiangbin ZHONG ; Zhenyu XIONG ; Yifen LIN ; Huimin ZHOU ; Yiquan HUANG ; Zhengzhipeng ZHANG ; Lichun WANG ; Xiaodong ZHUANG ; Xinxue LIAO
Diabetes & Metabolism Journal 2021;45(5):753-764
Background:
Currently available guidelines contain conflicting recommendations on the management of blood pressure (BP) in patients with diabetes mellitus (DM). Therefore, it is necessary to appraise the guidelines and summarize the agreements and differences among recommendations.
Methods:
Four databases and the websites of guideline organizations were searched for guidelines regarding BP targets and thresholds for pharmacologic therapy in DM patients, and the included guidelines were appraised with the Appraisal of Guidelines for Research and Evaluation (AGREE) II instrument.
Results:
In 6,498 records identified, 20 guidelines met our inclusion criteria with 64.0% AGREE II scores (interquartile range, 48.5% to 72.0%). The scores of the European and American guidelines were superior to those of the Asian guidelines (both adjusted P<0.001). Most of the guidelines advocated systolic BP targets <130 mm Hg (12 guidelines, 60%) and diastolic BP targets <80 mm Hg (14 guidelines, 70%) in DM patients. Approximately half of the guidelines supported systolic BP thresholds >140 mm Hg (10 guidelines, 50%) and diastolic BP thresholds >90 mm Hg (nine guidelines, 45%). The tiny minority of the guidelines provided the relevant recommendations regarding the lower limit of official BP targets and the ambulatory BP monitoring (ABPM)/home BP monitoring (HBPM) targets and thresholds in DM patients.
Conclusion
The lower official BP targets (<130/80 mm Hg) in patients with DM are advocated by most of the guidelines, but they contain conflicting recommendations on the official BP thresholds. Moreover, the gaps regarding the lower limit of official BP targets and the ABPM/HBPM targets and thresholds need to be considered by future study.
3.Optimization of culture method of mouse primary hippocampal neurons and construction of HT22-GRK2 -/ - cells
Menghui Guo ; Nana Xue ; Xi Yuan ; Qian Meng ; Wei Wei
Acta Universitatis Medicinalis Anhui 2023;58(4):589-596
Objective:
To explore and optimize the primary culture method of neonatal mouse hippocampal neurons in vitro.To construct a G-protein-coupled receptor kinase 2 ( GRK2) knockout HT22 cell line.
Methods :
Hippocampal tissue of C57BL6 /J mice on day 1-2 was taken,digested with trypsin and pipetted to form a cell suspension,and supplement was added to Neurobasal-A medium to maintain cell growth. CRSIPR / Cas9 gene editing technique was used to construct HT22-GRK2 -/ - cell line,and the knockout efficiency of GRK2 was detected by immunofluorescence staining and Western blot.
Results :
Primary hippocampal neurons of newborn mice were put into six-well plates with 3 × 107 /well using a serum-free culture method,which could get a high purity and good activity ; HT22-GRK2 -/ - cell line was constructed successfully.
Conclusion
The primary culture method of mouse hippocampal neurons was successfully established and optimized,and HT22-GRK2 -/ - cell line was successfully constructed by CRSIPR / Cas9 gene editing technique.