Objective:To investigate the status of mental health and work engagement of clinical nurses, to explore the correlation between them, and to provide scientific basis for improving the mental health and increase work engagement levels of nurses.Methods:From August to September 2018, 1 140 nurses in Taihe Hospital of Shiyan City of Hubei Province · Affiliated Hospital of Hubei University of Medicine were selected as survey objects by convenient sampling method, and the mental health and work engagement of nurses were investigated by Symptom Checklist-90 (SCL-90) and Utrecht Work Engagement Scale (UWES-9), correlation analysis was used to explore the relationship between mental health and work engagement, and multiple linear regression was used to analyze the influencing factors of work engagement in clinical nurses.Results:The score of SCL-90 of clinical nurses was 123.01±38.14 and the score of UWES-9 was 3.53±1.40. Correlation analysis showed that the nurse with total scores of mental health and work engagement, vitality, dedication, and concentration were negative correlation( r values were -0.250--0.218, P<0.01). Multiple linear regression analysis showed that age, depression, and paranoia were the influencing factors of clinical nurses work engagement ( t values were 4.682, -3.211, -2.715, P<0.01). Conclusions:The mental health level of nurses is good, and their work engagement level is average. Mental health is closely related to work engagement. Nursing managers should take effective measures to improve the mental health and increase work engagement levels of nurses.

Objective To explore the effect of nursing scientific research training based on system cybernetics.Methods The system cybernetics was applied in the scientific research training of 116 inservice nurses. The 4-level nursing scientific research management system which consisted of the mentor,research team,department head nurse and the director of nursing department was implemented. The key point of the training process was controlled from the aspects of training goal,curriculum content,teacher control,training method,information exchange,curriculum schedule and training effect. The scientific research ability,the scientific research quantity and the teaching satisfaction of nurses were compared between the data before and after training.Results After training,according to the scores of self-rating scientific research ability scale,the ability of finding problems (2.56±0.34),the ability of consulting literature (2.78±0.31),the ability of designing scientific researches (2.60±0.4),the ability of processing data (2.35±0.39),the ability of writing dissertation (2.41±0.37),the practice ability of scientific researches (2.38±0.29) of 116 inservice nurses were all significantly higher than the data before training (t=30.404,38.513,26.951,23.041,25.069,27.655;P<0.01). After training,the numbers of written dissertations (116),published papers (43),project applications (20), project approvals (8) were all higher than the data before training. The teaching satisfaction degree of nurses after training was higher than that before training.Conclusions The nursing scientific research training based on system cybernetics can effectively improve the scientific research ability of nurses and stimulate their studying enthusiasm.

Objective To analyze the effects and influencing factors of pre-job training on new nurses based on post competence so as to provide references for further post competence enhancement of new nurses.Methods A total of 106 new nurses in Taihe hospital from July 2014 to September 2014 received pre-job training based on post competence. Comprehensive theory, 'Three Basis' theory, operational skills and comprehensive quality in the department were evaluated after training. Multiple linear regression analysis was used to analyze the influencing factors.Results The scores of new nurses in different aspects from high to low were shown as following: comprehensive quality assessment (90.21±5.69), operational skill (86.32±6.73), comprehensive theory (85.27±5.39) and 'Three Basis' theory (83.27±5.47). Differences among nurses from different departments and with different education were statistically significant (P<0.05). Differences among nurses with different genders in comprehensive theory, 'Three Basis' theory and operational skills were statistically significant (P<0.05). Multiple linear regression analysis showed that department, gender and education affected comprehensive quality of new nurses.Conclusions Pre-job training of new nurses based on post competency help new nurses to adapt to job. Nursing managers should pay attention to learning effects and quality training of nurses in special departments, male nurses and nurses with low education level. Individual training is necessary to improve training quality of nurses.

OBJECTIVETo investigate the therapeutic effect of double fill nine tastes soup in treating children recurrent respiratory infection (RRTI) and the change of immune function.

METHOD77 RRTI patients were randomly selected into observation and control groups. The observation group was treated with Chinese medicine- double fill nine tastes soup,water frying points 2 times oral. The control was treated with transfer factor oral liquid,every 10 mL,2 times daily oral. Treatment periods were both two months. IgA, IgG, IgM and IL-12, TNF-alpha, INF-gamma were detected before and after treatment to assess the clinical effects and the changes of immune factors, meanwhile, a health group was established.

RESULTBefore treatment, compared with the health group, the serum IgA, IgG, IgM, IgE, IL-12, TNF-alpha, IFN-gamma in both groups were significantly different (P < 0.01). After treatment, the ratio of IgA, IgG, Ig M, IL-12, TNF-alpha, IFN-gamma in two groups were significantly different (P < 0.01). Compared with the recurrence rate and clinical effects, the observation group was better than control, and the differences were significant (P < 0.01).

CONCLUSIONDouble fill nine tastes soup has significant effects in treating recurrent respiratory infection (RRI) and enhance the immune function in children.

Child ; Child, Preschool ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Immunoglobulin G ; blood ; Immunoglobulin M ; blood ; Infant ; Interferon-gamma ; blood ; Interleukin-12 ; blood ; Male ; Respiratory Tract Infections ; blood ; drug therapy ; immunology ; Tumor Necrosis Factor-alpha ; blood

ObjectiveProteomics was used to investigate the protein differences between porcine cardiac blood（PCB） and porcine blood（PB） from Menghe medical school and to compare the effects of both on the microglial inflammation of Salviae Miltiorrhizae Radix et Rhizoma（DS）. MethodNanoliquid chromatography-quadrupole orbitrap mass spectrometry（nLC-MS/MS） and bioinformatics were utilized to compare the proteomic differences of PCB and PB in simulated gastrointestinal digestion. Furthermore， Western blot was used to verify the contents of some shared proteins and differential proteins identified in PCB and PB. In addition， BV2 neuroinflammation model constructed by corticosterone（CORT） and lipopolysaccharide（LPS） was applied to detect the intervention effects of PCB and PB on the levels of tumor necrosis factor（TNF）-α and interleukin（IL）-6 in BV2 inflammatory cells of DS. ResultA total of 69 common proteins and 68 differential proteins were identified in PCB and PB， among which the common proteins included transferrin（Tf） with brain-targeting effect， and the differential proteins in the two were 41 and 27， respectively. Western blot validation showed that the difference in the content of the same protein Tf between PCB and PB was not statistically significant， while the difference in the contents of the specific proteins of creatine kinase M and heart-type fatty acid binding protein（H-FABP） were statistically significant（P<0.05）. Moreover， in vitro experimental studies revealed that compared with the same concentration of DS group， in addition to the 100 mg·L^-1 PB-DS group， PCB-DS and PB-DS groups could significantly inhibit the levels of TNF-α and IL-6 in BV2 inflammatory cells（P<0.05， P<0.01）， and PCB-DS group had more significant anti-inflammatory effect than PB-DS group with the same concentration（P<0.05， P<0.01）. ConclusionBoth of PCB and PB can enhance the inhibitory effect of DS on the release of inflammatory factors， thus playing a neuroprotective role， and PCB promotes DS inhibition more significantly， which may be due to the existence of the two involved in energy metabolism-related differential proteins， which can lay a foundation for revealing the scientific connotation of the processing of PCB-DS and PB-DS.

ObjectiveTo analyze the correlation between 11 small molecule active components and 1 protein component of characteristic processed products with porcine cardiac blood and other products of Salviae Miltiorrhizae Radix et Rhizoma（SMRR） from Menghe medical school and anti-cerebral ischemic oxidative damage， and to identify its key component markers of characteristic processed products with porcine cardiac blood for anti-cerebral ischemic oxidative damage. MethodHigh performance liquid chromatography（HPLC） was established to simultaneously determine the contents of 11 active ingredients in SMRR and its processed products［processed with porcine cardiac blood， porcine blood， wine and transferrin（Tf） in porcine cardiac blood］， and the content of Tf in different processed products of SMRR was detected by enzyme-linked immunosorbent assay（ELISA）. Furthermore， A zebrafish ischemic stroke model was constructed to evaluate the effects of different processed products of SMRR on the behavioral trajectory of cerebral ischemic zebrafish， the neuronal damage of transgenic zebrafish Tg（elavl3：eGFP） brain， as well as the levels of malondialdehyde（MDA） and superoxide dismutase（SOD） in the brain tissues. The hippocampal neurons oxygen-glucose deprivation/reoxygenation（OGD/R）-induced ischemia-hypoxia model was constructed to evaluate the effects of different processed products of SMRR on oxidative damage of neuronal cells by taking lactate dehydrogenase（LDH）， reactive oxygen species（ROS）， MDA and SOD as indexes. Finally， principal component analysis（PCA）， partial least squares-discriminant analysis（PLS-DA） and Spearman correlation analysis were used to analyze the 11 small molecule active components and 1 protein component with efficacy indicators， in order to screen the key components of the characteristic processed products with porcine cardiac blood for cerebral ischemic oxidative damage. ResultCompared with the raw products， the contents of water-soluble and fat-soluble components in processed products of SMRR increased to different degrees， while the content of salvianolic acid A decreased. Compared with the wine-processed products， the contents of salvianolic acid B， danshensu， rosmarinic acid and other components in the porcine cardiac blood-processed products， porcine blood-processed products， Tf-processed products were increased， while the content of salvianolic acid A was decreased. ELISA results showed that there was no significant difference in Tf content between the porcine cardiac blood-processed products， porcine blood-processed products， Tf-processed products. Pharmacological results showed that different processed products of SMRR could improve the behavioral deficits， brain neuronal injury and oxidative stress after ischemic stroke in zebrafish， and the effect of the porcine cardiac blood-processed products was most pronounced. PCA results showed that salvianolic acid B， salvianolic acid A， rosmarinic acid， lithospermic acid， danshensu， tanshinone Ⅱ_A， caffeic acid， cryptotanshinone and tanshinone Ⅰ were the main contributing components of SMRR and its processed products. And the results of correlation analysis showed that the contents of cryptotanshinone， rosmarinic acid， caffeic acid， dihydrotanshinone Ⅰ， salvianolic acid B， tanshinone Ⅱ_A and tanshinone Ⅰ were negatively correlated with MDA level in zebrafish brain tissue， while the contents of lithospermic acid， protocatechuic aldehyde， rosmarinic acid， dihydrotanshinone Ⅰ， salvianolic acid B and Tf were positively correlated with SOD level， and the contents of rosmarinic acid， caffeic acid， dihydrotanshinone Ⅰ， salvianolic acid B， tanshinone Ⅱ_A， tanshinone Ⅰ， danshensu， Tf were positively correlated with neuronal fluorescence intensity in the zebrafish brain. And the contents of lithospermic acid， protocatechuic aldehyde， rosmarinic acid， dihydrotanshinone Ⅰ， salvianolic acid B， tanshinone Ⅱ_A and Tf were negatively correlated with LDH， ROS and MDA levels and positively correlated with SOD level. ConclusionThere are differences in the anti-ischemic oxidative damage effects of SMRR and its different processed products， among which the porcine cardiac blood-processed products has the strongest effect on improving oxidative damage， which may be related to the content changes of salvianolic acid B， danshensu， rosmarinic acid and other components. This study can provide a basis for clarifying the quality markers of SMRR processed with porcine cardiac blood for cerebral ischemia and elucidating its processing mechanism.

Objective To explore the effects of nursing specialty construction on vascular crisis in patients after replantation of severed finger.Methods A case control study was conducted, and 151 patients ( 203 fingers) of microsurgery in a hospital from January to December 2015 were selected as the observation group, and 143 patients ( 195 fingers) were selected as the control group. Patients in the control group received routine postoperative nursing, while patients in the observation group were given special technologies and special nursing measures which included " self-help pain management techniques""decubitus nursing technology" " warm comfortable security technologies" " cut flush bloodletting technologies" and "five-love service" through the construction of special nursing specialty; SPSS 17. 0 statistical software was used to analyze the incidence rate of vascular crisis in two groups. Results In the observation group, 151 cases, 203 fingers received replantation, and the incidence rate of arterial crisis was 2.0%;the incidence rate of venous crisis was 2.5%. In the control group, 143 cases, 195 fingers received replantation and the incidence rate of arterial crisis was 7.7%;the incidence rate of venous crisis was 9.2%. According to the statistical analysis, there was significant difference between two groups ( P<0. 05 ) . Conclusions Through the construction of nursing specialty, the implementation of special nursing technology and special nursing service, can effectively reduce the incidence of vascular crisis and improve the innovation ability and professional level of the nurses, and promote the sustainable development of nursing.

Dysphagia is one of the widespread diseases in the aged which can lead to severe complications, such as aspiration pneumonia, even asphyxia. Therefore, early screening of dysphagia is vital for the aged. This paper reviewed the dysphagia screening scale for the aged at home and abroad so as to provide a reference for clinical workers to select the dysphagia screening scale and a thinking for the development of dysphagia risk assessment tools for the aged in China.

45 strains of Lactobacillus were isolated from 17 samples of traditional fermented milk in Gobi region of Mongolia. Based on morphological, physiological, biochemistry test and 16S rDNA sequencing analysis, identified as 31 strains of Lactobacillus fermentum(L. fermentum), 12 strains of L. helveticus, one strain of L. plantarum and one strain of L. casei. Survival rate of IMAU20085 is 81.44% in the screening experiment of resistance to the artificial gastric juice (pH 3.0). The isolation and identification of these strains and the screening of high acid-tolerant strains have important meaning to the preservation and exploitation of probiotic resource.

Objective To explore the effect of competency-oriented pre-service training for new nurses. Methods A total of 85 new nurses in our hospital in 2013 were selected as the research object and carried out the pre-service training needs survey. According to the survey and the position competency, we developed the training program. The effectiveness of the training were evaluated and analyzed. Results A total of 88. 3% of the new nurses needed the pre-service training, and the content that new nurses most wanted were cardiopulmonary resuscitation ( 80. 0%), specialized knowledge and skills ( 76. 5%), nurse-patient communication skills (72. 9%). The training methods that the new nurses would like to accept were multimedia lectures on theoretical knowledge ( 75. 3%), basic skills training for the integrated simulation scenario (62. 4%), specialized training for case studies (72. 9%). The satisfaction of theoretical training of the nursing department and other department, skills training of the nursing department and other department, training method of the nursing department and other department of 2013 were significantly better than those of 2012 (χ2 =23. 395, 20. 588, 17. 998, 9. 532, 11. 173, 21. 564, 14. 564, 10. 188, respectively;P<0. 01). The examination results of pre-service training of new nurses in 2013 were (87. 5 ± 4. 8),(89. 6 ± 4. 9),(88. 3 ± 5.4),(86. 2 ± 4. 7),which were significantly better than those in 2012 (t =6. 472, 5. 753, 4. 468, 6. 868, respectively;P<0. 01). Conclusions The competency-oriented pre-service training for new nurses is beneficial to improve the quality of training, and improve the comprehensive quality of new nurses and position competency.