Objective To develop a portbable incubator for on-site cultivation of microorganisms in foods and drinking water.Methods Cultivation temperature was set as required by the temperature for various microorganisms and PID was controlled via the single chip microcomputer and configuration screen .Then, the framework of the incubator was designed and assembled.Finally, the cultivation effect was tested .Results The incubator was compact and portable .The deviation of the temperature was in the range of 1℃.The hold time of self-contained power could exceed 8 h.In addition, the cultivation effect of our fabricated incubator was not significantly different from that of the commercial electro-heating standing-temperature cultivator used in laboratories .Conclusion The incubator is suitable for on-site detection of microorganisms in foods and drinking water , which is significant for spotting and removing the hidden dangers from microorganism contaminations in foods and drinking water in order to protect the health of soldiers .

BackgroundProlonged grief disorder （PGD） has serious impact on the quality of patients' work and life. Previous relevant studies have explored the intervention effect of cognitive behavioral therapy（CBT） on PGD， but their conclusions remain controversial due to differences in frequency， time and content of intervention. ObjectiveTo explore the intervention effects of CBT on the PGD through Meta-analysis， so as to provide references for clinical treatment. MethodsOn October 22， 2022， the database of VIP， China Biomedical Literature Database， Wanfang， CNKI， Embase， Cochrane Library， PubMed and Web of Science were searched systematically， and randomized controlled trials （RCTs） of CBT in treating PGD was collected. Outcome indicators include sadness， depression， anxiety and somatization symptoms. RevMan 5.3 was used for Meta-analysis. ResultsA total of 7 articles were included with a total sample size of 528 cases. Meta-analysis results showed that the study group had lower scores than the control group for grief （SMD=-0.78，95% CI：-1.27~-0.29）， depression （SMD=-0.45，95% CI：-0.73~-0.17） and anxiety symptom （SMD=-0.38，95% CI：-0.59~-0.18） with statistically significant differences. However， the difference of scores for somatization symptom between two groups was not significant （SMD=-0.01，95% CI：-0.26~0.25）. ConclusionCBT may contribute to improving the symptoms of grief， depression and anxiety in PGD patients but unsatisfactory efficacy on somatization symptom.

Objective:To construct a recombinant bioluminescent bacteriophage (HT7) targeting Escherichia coli, and evaluate its ability to identify Escherichia coli. Methods:Initially, pCRISPR-sg (1-10) and PFN-1000 plasmid strains were constructed by genetic engineering, and the most efficient small guild RNA (sgRNA) were screened by bilayer plate. By the gene editing technique, which comprised homologous recombination and clustered regularly interspaced short palin dromic repeats (CRISPR)-Cas system, the Nanoluc luciferase gene was integrated into the downstream non-coding region of 10A gene of T7 phage, to constructe the bioluminescent phage HT7 successfully. The difference of biological characteristics between HT7 phage and T7 phage was evaluated by plaque assay and liquid amplification assay. In addition, 51 strains of Escherichia coli, 20 strains of Klebsiella pneumoniae, 14 strains of Staphylococcus aureus, 6 strains of Enterococcus faecium, 5 strains of Enterococcus faecalis, 3 strains of Acinetobacter baumannii and 1 strain of Pseudomonas aeruginosa were collected and isolated to evaluate the limit of detection and specificity of HT7 phage. Results:Among the 10 CRISPR-targeted cleavage systems constructed, sgRNA8 exhibited the highest cleavage efficiency, with a cleavage rate of 0.18. After three rounds of recombination screening using the pCas9/pCRISPR/PFN-1000 triple-plasmid system, PCR validation yielded recombinant phage bands at 2 798 bp, indicating the successful construction of the HT7 phage. The recombinant phage showed significant differences in biological characteristics in terms of lysis efficiency ( P<0.001), one-step growth curve ( P=0.001), and infection multiplicity ( P=0.031). Both lysis burst time and log growth node were extended by 10 min, with the optimal infection multiplicity being 0.1. Clinical sample testing identified lysis of 6 strains of Escherichia coli within 4.5 h, while other strains remained unaffected, with detection of pathogenic bacteria below 10 CFU/ml. Conclusions:The developed pCas9/pCRISPR/PFN-1000 triple-plasmid editing system efficiently edits the bacteriophage genome. The constructed HT7 fluorescent bacteriophage enables the detection of Escherichia coli below 10 CFU/ml within 4.5 hours, demonstrating low detection limits and high detection specificity.

ObjectiveTo clarify the relationship between childhood maltreatment and insomnia in middle school students， and to explore the mediating role of post-traumatic stress disorder （PTSD） symptoms and the moderating role of mental resilience involvement. MethodsFrom April to May 2021， a total of 3 412 students in 3 middle schools in a city in western China were selected using convenient sampling method， and all students were assessed by Childhood Trauma Questionnaire （CTQ）， Post Traumatic Stress Disorder Scale （PCL）， Insomnia Severity Index （ISI） and mental resilience scale. The moderated mediating effect analysis was conducted using Process v3.4. ResultsAmong the middle school students， CTQ scores were positively correlated with PCL and ISI scores （r=0.540， 0.320， P<0.05）， and the scores of CTQ， PCL and ISI were negatively correlated with the score of mental resilience scale （r=-0.049， -0.193， -0.132， P<0.05）. PTSD symptoms exhibited a partial mediating effect on the relationship between general childhood maltreatment and insomnia （β=0.161， P<0.05）， accounting for 80.5% of the total effect. ConclusionAmong the middle school students， the relationship between childhood maltreatment and insomnia is partially mediated via PTSD symptoms， and mental resilience exerts a moderating role between childhood maltreatment and PTSD symptoms.

Objective : To investigate the effect of growth hormone (GH) pretreatment on the improvement of euploid and pregnancy outcome . Methods : A prospective analysis was conducted on 134 patients undergoing preimplantation genetic testing for aneuploidy(PGT⁃A) , among whom 30 patients were self⁃controlled and 104 patients were inter⁃group controlled . According to whether GH was added , the patients were divided into GH pretreatment group and GH non⁃pretreatment group . GH pretreatment included subcutaneous injection of GH 2U/day for 4 - 6 weeks before the start of gonadotropin (Gn) , and the dose was doubled on the day of Gn until the trigger day . GH non⁃pretreatment meant no GH treatment , GH pretreatment was given when the previous PGT⁃A cycle failed within one year when the PGT⁃A was performed again , forming the self⁃control group . The basic situation , blastocyst situation and pregnancy outcome were compared between the groups by inter⁃group and self⁃control . Results : No matter in the group control or self⁃controlled group , the endometrial thickness on the day of HCG , ovarian sensitivity index ( OSI) , number of oocytes obtained , MII oocytes , 2PN number , 2PN fertilization rate , available oocyte rate , number of biopsy blastocysts , number of euploid blastocysts , euploid blastocyst rate , and at least one euploid rate significantly increased after GH pretreatment , with statistically significant differences (P < 0. 05) . The total amount of Gn , Gn days , number of mosaic blastocysts , and mosaic blastocyst rate were not significantly changed after GH pretreatment , with no statistically significant differences . The implantation rate and clinical pregnancy rate increased after GH pretreatment , but with no statistically significant differences . Conclusion GH pretreatment can significantly improve the number and rate of euploid embryos in patients undergoing PGT⁃A , and has a tendency to improve pregnancy outcome .