Objective:To construct and evaluate a novel tissue-engineered bone composed of murine stromal cell-derived factor 1(mSDF-1), simvastatin (SIM) and collagen scaffold (Bio-Oss?), serving as a cell-homing approach for bone formation .Methods: In the study , 32 ICR mice were randomly divided into 4 groups,each group including 8 mice.The drug-loaded collagen scaffolds were implanted subcutaneously onto the cranium of each mouse according to the groups: ( 1 ) 1 ∶50 ( volume ratio ) dimethyl sulfoxide ( DMSO ) /phosphate-buffered saline ( PBS ) solution +collagen scaffold ( blank control group ); ( 2 ) 10 -3 mol/L SIM solution +collagen scaffold ( SIM group ); ( 3 ) 200 mg/L mSDF-1solution +collagen scaffold (mSDF-1 group); and (4) 10 -3mol/L SIM +200 mg/L mSDF-1 solution +collagen scaffold ( SIM +mSDF-1 group) .One week after implantation , the mice were trea-ted by injecting the same drug solution mentioned above around the scaffold once a day for two days .The specimens were harvested 6 weeks after implantation and the bone formation was evaluated by soft X-ray analysis , HE staining and immunohistochemical staining .Angiogenesis of each group was checked by calculation of vessels in each tissue section .Results:Six weeks after implantation , the collagen scaffolds were retrieved.The value of gray scale for the SIM +mSDF-1 group[(421 836.5 ±65 425.7) pixels] was significantly higher than that of the blank control group [(153 345.6 ±45 222.2)pixels, P<0.01], the SIM group [(158 119.2 ±100 284.2) pixels, P<0.01], and the mSDF-1 group[(255 529.5 ± 152 142.4) pixels, P <0.05 ]; HE staining analysis revealed that significant bone formation was achieved in the SIM +mSDF-1 group; The immunohistochemical staining showed the existence of os-teopontin and osteocalcin in the SIM +mSDF-1 group; There were more vessels in the SIM +mSDF-1 group[(46 ±8)vessels/mm2] than in the blank control group [(23 ±7) vessels/mm2, P<0.01], and the SIM group[(24 ±6) vessels/mm2 , P<0.01].Conclusion:The novel tissue-engineered bone com-posed of mSDF-1, SIM and collagen scaffolds has the potential to form bone subcutaneously in vivo.It re-presents a novel method of in vivo bone re-generation without seed cell delivery .

Objective To explore the mechanism underlying the anti-hemorrhoid effects of Qingji Liangxue mixture(QLM).Methods Models of acute hemorrhoid in rats were established using a croton oil preparation(COP)and glacial acetic acid.A total of 80 SD female rats were randomly divided into two major groups:the COP group and the glacial acetic acid group,each consisting of 40 rats.In the COP group,rats were further randomly assigned to the following subgroups:control group 1(group CA),model group 1(group CB),low-dose QLM group 1-1(group CC),high-dose QLM group 1-2(group CD),and diosmin group 1(group CE),with eight rats in each subgroup.In the glacial acetic acid group,rats were randomly divided into the following subgroups:control group 2(group GA),model group 2(group GB),low-dose QLM group 2-1(group GC),high-dose QLM group 2-2(group GD),and diosmin group 2(group GE),with eight rats in each subgroup.Except for the control groups,all other subgroups were treated with COP or glacial acetic acid then treated with various drugs for 7 days.HE staining was used to observe anorectal histomorphology induced by COP and glacial acetic acid.ELIS As were used to measure serum levels of IL-6 and TNF-α induced by COP.Additionally,the area of perianal ulcers induced by glacial acetic acid was recorded.Twenty-four C57 mice were randomly divided into a control group(group A),low-dose QLM group(group B),high-dose QLM group(group C),and adrenal chromazone tablet group(group D).Results QLM significantly improved the pathological injury of animal models with similar hemorrhoids,reduced the score of pathological changes induced by COP(P＜0.05),reduced the serum levels of IL-6 and TNF-α(P＜0.05),reduced the area of perianal ulcers induced by glacial acetic acid(P＜0.05),and shortened the time of coagulation and bleeding(P＜0.05).Conclusions QLM has a good anti-hemorrhoid activity,which may be achieved by anti-inflammatory effects,hemostasis,and reducing tissue damage.

ObjectiveTo observe the clinical effect of modified Qilang prescription on the treatment of drug-dependent constipation of Qi-Yin deficiency type. MethodIn a randomized double-blind clinical trial， 160 patients with drug-dependent constipation were randomly divided into two groups， namely， the treatment group （80 cases） and the control group （80 cases）. The treatment group was treated with modified Qilang prescription and lactulose oral liquid simulator， while the control group was treated with lactulose oral liquid and the simulator of modified Qilang prescription. The course of treatment of both groups was 8 weeks. The scores of main syndromes of constipation， traditional Chinese medicine （TCM） syndromes， patient assessment of constipation symptom （PAC-SYM） scale， and patient assessment of constipation quality of life （PAC-QOL） scale before and after treatment were recorded. The patients were followed up at the 2^nd and 4^th week after drug withdrawal. ResultAfter treatment， the full analysis set （FAS） analysis showed that the total effective rate was 91.14% （72/79） in the treatment group and 73.33% （55/75） in the control group. The treatment group had a higher total effective rate than the control group （Z=-6.62， P<0.01）. The per-protocol set （PPS） analysis showed that the total effective rate was 93.51% （72/77） in the treatment group and 76.39% （55/72） in the control group. The treatment group had a higher total effective rate than the control group （Z=-6.77， P<0.05）. After treatment， the FAS and PPS analysis showed that the main syndrome scores of constipation were lower in both groups than those before treatment （P<0.05）. Except the syndromes of falling， overexertion defecation， and distension， the scores of other syndromes in the treatment group were lower than those in the control group （P<0.05）. The scores of TCM primary syndromes in the two groups were lower than those before treatment （P<0.05）， and the scores of the treatment group were significantly lower than those of the control group （P<0.05）. The scores of TCM secondary syndromes in the treatment group were significantly lower than those before treatment （P<0.05）. The syndrome scores of abdominal distension， fatigue， and lack of sleep in the treatment group were significantly lower than those in the control group （P<0.05）. The scores of PAC-SYM scale were improved in both groups after treatment （P<0.05）， and the treatment group was superior to the control group in improving fecal syndromes and abdominal syndromes. The scores of PAC-QOL scale were improved in both groups after treatment， and the treatment group was superior to the control group in improving patients’ physiological function， social psychology， anxiety， and satisfaction. After drug withdrawal， the recurrence rate was lower in the treatment group at the 2^nd（χ² =5.65，P<0.05） and 4^th week （χ² =12.37，P<0.01）. Conclusionmodified Qilang prescription is effective in the treatment of drug-dependent constipation， and its clinical effect is better than that of lactulose oral liquid. The curative effect of modified Qilang prescription is stable and lasting without obvious adverse reactions.