1.Research on the innovative mode of comprehensive ability training for the Chinese medicine majors based on the combination of "multi-mode and multi-ways"
Han ZHANG ; Yan ZHANG ; Xingjun WANG ; Xuji SHEN ; Mengdi ZHENG
International Journal of Traditional Chinese Medicine 2018;40(6):551-553
Chinese medicine talents are the foundation and guarantee for the development of TCM, and the first resource for the inheritance and innovation of traditional Chinese medicine. Reforming the curriculum system and training mode of Chinese medicine, training Chinese medicine professional who are suitable for research and development of Chinese medicine, such as cultivation of Chinese medicine materials, quality detection, species identification and resource census, is the direction of further expansion and reformation of Chinese medicine education. In this article, on account of the characteristic of Chinese medicine professional courses, we will combine practice education mode with professional knowledge competition, to probe innovative mode of comprehensive ability training for Chinese medicine based on the combination of"Multi-mode and Multi-Ways". The new mode of Chinese medicine education is to provide reference for trainers of Chinese medicine.
2.Gas chromatography-mass spectrometry analysis of volatile oil chemical composition in perilla leaf and perilla stem
Yan ZHANG ; Bo CHEN ; Qiang LIU ; Mengdi ZHENG ; Yimei TANG ; Hongjuan TONG ; Xifeng ZHAI ; Jing LIU
International Journal of Traditional Chinese Medicine 2020;42(7):679-682
Objective:To explore the difference of constituents and contents of volatile oil in Perilla leaf and Perilla stem.Methods:The volatile oils of Perillaleaf and Perillastems were extracted with steam distillation method. Gas chromatography-mass spectrometry (GC-MS) technique was used to detect the chemical constituents of volatile oil from different parts of Perilla frutescens. The relative percentage content of each component was determined by peakarea normalization method, and the differences of the components and contents of volatile oils from different parts of Perilla frutescens were compared.Results:A total of 24 volatile oil components were identified in perilla leaves and 19 volatile oil components in Perilla stems. The most components in Perilla leaf and Perilla stems was 2-Hexanoyl furan. The content of 2-Hexanoyl furan in the oil of Perilla leaf reached 67.493%. The content of 2-Hexanoyl furan in the oil of Perilla stem reached 70.473%.Conclusions:The composition and content of volatile oil in different parts of Perillafrutescens are different.
3.Exploration of creating a Chinese materia medica teaching system based on the Qinling plant gene database
Yan ZHANG ; Han ZHANG ; Xingjun WANG ; Xuji SHEN ; Mengdi ZHENG ; Caixia WEI ; Yonghui FENG
International Journal of Traditional Chinese Medicine 2019;41(2):203-205
Qinling is a treasury of medicine with abundant resources of herbs. Due to the goal of enriching teaching contents, quality of teaching and creating a Chinese materia medica teaching system. Based on the Qinling plant gene database, our education team presents a series of innovative thoughts, such as multidisciplinary contact, inheriting and carrying forward the folk medicine, attaching importance to the practical application of herbs in Qinling and theory with practice to enrich teaching contentsand creating Shanxi characteristic teaching system of Chinese materia madica.That makes a contribution to raise the level of teaching in modern traditional Chinese medicine and promote the development of medicine in Shannxi.
4.Study on the techniques of purification flavones from Gnaphalium japonicum Thunb. by D-101 macroporous adsorption resin
Mengdi ZHENG ; Yong WANG ; Xingjun WANG ; Yan ZHANG ; Xuji SHEN ; Han ZHANG
International Journal of Traditional Chinese Medicine 2018;40(10):965-969
Objective To investigate the optimal isolation and purification conditions of flavonoids in Gnaphlium affine Thunb. and the content of flavonoids in different parts of Gnaphlium affine Thunb. was measured. Methods The separation and purification abilities of D-101 macroporoue adsorbing resins for flavonoids in Gnaphlium affine Thunb. were studied with adorption and desorption as index. The static adsorption and dynamic adsorption methods were used to analyze the effects of static saturated adsorption, static elution rate, sample concentration, sample pH value, eluent concentration and amount of eluent. The flavonoids concentrations were determined with rutin as standard. Results The D-101 macroporous adsorption resin had good effect on the separation and purification of flavonoids from Gnaphlium affine Thunb.; The optimal conditions for purification were: sample concentration 1.0 mg/ml, sample pH=4, 60% ethanol as desorption solvent, washing flow 2 BV/h, with these parameters. With such condition, the purity of flavonoids in Gnaphlium affine Thunb. was 60.92%; The content of flavonoids in Gnaphlium affine Thunb. showed the highest content was in the stem, the second in the flower, and the least in the root. Conclusions The purification of flavonoids from the D-101 macroporous adsorption resin increased in the best purified separation conditions and the content of flavonoids was the highest in the leaf.
5.GC-MS analysis of volatile oil chemical composition in the leaves of phlomis umbrosa from different habitats
Yan ZHANG ; Yimei TANG ; Mengdi ZHENG ; Qiang LIU ; Xifeng ZHAI ; Jie ZHANG
International Journal of Traditional Chinese Medicine 2020;42(5):464-468
Objective:To explore the composition and content differences of volatile oil in the leaves of Phlomis umbrosa from different habitats, in order to clarify the chemical composition and content on efficacy.Methods:To collect the Phlomis umbrosa from Haoping area in Mei county and Sanyuan county of Shaanxi province. The volatile oil of Phlomis umbrosa was extracted by steam distillation method. Gas chromatography- mass spectrometry (GC-MS) was used to detect the chemical constituents of volatile oil.Results:Forty-nine kinds of volatile components were identified from the leaves collected in Haoping area of Mei county and fifty-six volatile component were identified from the leaves collected in Sanyuan county. There are five ingredients in common, excluding diacetone alcohol. 2-tert-butoxy-5-methylthiophene is the main volatile oil composition and the content reached 18.009% of leaves collected from Haoping area in Mei county, while paclitaxel is main volatile oil composition and the content reached 50.728% in Sanyuan county.Conclusions:The chemical compostion and content of volatile oil of Phlomis umbrosa in different habitats are different.
6.Clinical significance of expression of leptin in patients with biliary atresia and hepatic fibrosis
Qiong WANG ; Qipeng ZHENG ; Cong ZHANG ; Lingzhi CHEN ; Mengdi LI ; Renjie YANG ; Fangyuan ZHAO ; Yingyi QI ; Wenfan XUE ; Jianghua ZHAN
Chinese Journal of Hepatobiliary Surgery 2022;28(4):275-279
Objective:To study the relationship and the role of leptin in children with biliary atresia and hepatic fibrosis to provide a treatment basis for these patients.Methods:The clinical data of children with biliary atresia or congenital biliary dilatation (CBD) who underwent surgical treatment at the Department of General Surgery of Tianjin Children's Hospital from August 2019 to August 2021 were retrospectively analyzed. Of 31 children included in this study, there were 14 males and 17 females, with age of 60 (30, 63) d. Children with biliary atresia served as the study group ( n=26) and children with CBD served as the control group ( n=5). Leptin protein, α-smooth muscleactin (α-SMA) and phosphorylation of extracellular-regulated protein kinase 1/2 (p-ERK1/2) in liver tissues were detectd by immunohistochemistry (IHC). The expression level of leptin mRNA in liver tissues were detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Results:The average optical density values of leptin protein, α-SMA protein and p-ERK1/2 protein in the liver tissues of children in the study group were significantly higher than the control group ( P<0.05). The expression levels of leptin, α-SMA and p-ERK1/2 in liver tissues of children with biliary atresia significantly increased with increase in fibrosis degree ( P<0.05). The expression level of leptin in liver tissues of children with biliary atresia was positively correlated with the liver fibrosis grade ( rs=0.876), α-SMA ( r=0.723) and p-ERK1/2 ( r=0.725) ( P<0.01). The results of qRT-PCR showed that the content of leptin mRNA in liver tissues of children with biliary atresia was significantly higher than that of children with CBD ( P<0.05). Conclusion:Expressions of leptin increased with aggravation of degrees of hepatic fibrosis in biliary atresia. Leptin may be involved in activation of HSCs through the ERK1/2 signaling pathway in the process of hepatic fibrosis due to biliary atresia.
7. Efficacy and safety of rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen in eradicating refractory Helicobacter pylori infection
Mengdi LI ; Zinan WANG ; Songbai ZHENG
Chinese Journal of Digestion 2019;39(12):812-816
Objective:
To observe the efficacy and safety of rifabutin and furazolidone included triple regimen and minocycline plus furazolidone included quadruple regimen in eradicating refractory
8.Effects of Acupuncture on Gut Microbiota and Central Iinflammation in Rat Model of Migraine based on"Gut-Brain"Theory
Mingsheng SUN ; Xingyu CHEN ; Mengdi ZHOU ; Yi LIU ; Yuqi ZHENG ; Siying HUANG ; Dingjun CAI ; Ling ZHAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(8):2726-2734
Objective Based on the theory of"gut-brain",this study explored the effect of acupuncture on the gut microbiota and central inflammation in migraine model rats,in order to explore the mechanism of acupuncture in the treatment of migraine from the perspective of"gut-brain".Methods The migraine rat model was established by subcutaneous injection of nitroglycerin.They were randomly divided into a model group and an acupuncture group,with 6 rats in each group,and a control group with 6 rats for conventional binding and fixation.Before modeling and on the 1st,5th,and 9th days after modeling,each group used electronic VonFrey to measure the plantar mechanical pain threshold of rats.After the experiment,Elisa was used to detect the expression levels of inflammatory factors IL-6 and TNF-α in the central trigeminal spinal nucleus of the rats in each group.Three-generation Pacbio full-length microbial diversity sequencing was used to perform 16S full-length rDNA sequencing on each group of fecal samples to detect the operational taxonomic unit(OTU)clustering and its abundance,Alpha diversity index,Beta diversity index,species among the samples in each group.differences in abundance.Results In migraine model rats,plantar mechanical pain threshold was significantly decreased(P<0.01),central IL-6 and TNF-α contents were significantly increased(P<0.01),and the structure and abundance of gut microbiota were abnormal.change(P<0.01).Continuous acupuncture treatment can significantly increase the plantar mechanical pain threshold in migraine rats(P<0.01),regulate the diversity of gut microbiota in migraine rats,increase Lactobacillus murine,and reduce the abundance of Lactobacillus enterobacteriaceae.degree(P<0.05),and decreased the levels of IL-6 and TNF-α in the central nervous system of migraine model rats(P<0.01).Conclusion Acupuncture can exert the"gut-brain"anti-inflammatory and analgesic effect of acupuncture in the treatment of migraine by regulating the gut microbiota structure and the expression of central IL-6 and TNF-α inflammatory factors in migraine model rats.
9.The lesion characteristics and predictors of obstructive coronary artery disease with anatomy-function mismatch
Mengdi JIANG ; Xiaolei ZHANG ; Yang HOU ; Minwen ZHENG ; Jiayin ZHANG ; Bo ZHANG ; Daimin ZHANG ; Lei XU ; Xiuhua HU ; Yining WANG ; Jian YANG ; Hui LIU ; Fan ZHOU ; Guifen YANG ; Longjiang ZHANG
Chinese Journal of Radiology 2021;55(12):1287-1293
Objective:To explore the lesion characteristics and predictors of invasive coronary angiography (ICA)-verified obstructive lesions with fractional flow reserve (FFR)>0.80, that is, anatomy-function mismatch.Methods:A total of 515 obstructive vessels in 419 coronary disease patients from 11 Chinese medical centers undergoing coronary CT angiography and ICA and FFR were retrospectively analyzed. All vessels had one target lesion with diameter stenosis ≥50 % by ICA. There were 229 vessels in the match group (FFR≤0.80) and 286 vessels in the mismatch group (FFR>0.80). The lesion characteristics including lesion territory, the distance of the coronary artery ostium to the proximal end of the lesion, minimum lumen area, reference lumen area, plaque length and burden, plaque volume and component volume, remodeling index and plaque morphological complexity were measured and compared between the two groups. Optimal thresholds of quantitative plaque characteristics were defined by Yoden index. Logistic regression analysis was used to analyze the predictors of anatomy-function mismatch. Area under receiver operating characteristic curve (AUC) was used to analyze the ability of different lesion features to predict mismatched lesions.Results:The coronary stenosis, plaque burden and length, plaque volume (including each component volume) in the mismatch group were smaller than those in the match group, and FFR, minimum lumen area were larger (all P<0.05). Left anterior descending artery (LAD) lesion and severe complex plaque were more common in the match group than the mismatch group with a statistically significant difference. Univariate logistic regression analysis showed that LAD lesion, minimum lumen area>4 mm 2, plaque burden and length, plaque calcification volume<27 mm 3, plaque lipid volume<30 mm 3, plaque fiber volume<150 mm 3 and plaque morphological complexity were predictiors of anatomic function mismatched lesions; Multivariate logistic regression showed that the minimum lumen area>4 mm 2 (OR=3.371, 95%CI 1.903-5.973, P<0.001), plaque lipid volume<30 mm 3 (OR=3.014, 95%CI 1.691-5.373, P<0.001), plaque morphological complexity (mild OR=17.772, 95%CI 8.072-39.128, P<0.001, moderate OR=6.383, 95%CI 3.739-10.896, P<0.001) were independent predictors of mismatched lesions. The AUC of the model based on the minimum lumen area, plaque lipid volume and morphological complexity was 0.824, which was superior to either of the plaque feature alone ( P<0.001). Conclusions:The minimum lumen area, lipid volume and plaque morphological complexity are independent predictors of the anatomical-functional mismatch lesions, and the combination can significantly improve the prediction value.
10.Inhibition of subicular seizure-labeled c-fos+ neurons alleviates cognitive deficit in epilepsy
Lin YANG ; Qi ZHANG ; Xueqing WU ; Xiaoyun QIU ; Fan FEI ; Nanxi LAI ; Yuyi ZHENG ; Mengdi ZHANG ; Qingyang ZHANG ; Yu WANG ; Fei WANG ; Cenglin XU ; Yeping RUAN ; Yi WANG ; Zhong CHEN
Chinese Journal of Pharmacology and Toxicology 2023;37(7):482-483
OBJECTIVE Cognitive deficit is a com-mon comorbidity in temporal lobe epilepsy(TLE)and that is not well controlled by current therapeutics.Currently,how epileptic seizure affects cognitive performance remains largely unclear.The subiculum is the major out-put of the hippocampus,which projects to entorhinal cor-tex and other more distinct brain regions.Physiologically,the subiculum codes spatial working memory and naviga-tion information including place,speed,and trajectory.Importantly,prior studies have noted the importance of the subiculum in the beginning,spreading,and generaliz-ing process of hippocampal seizure.How seizure-activated neurons in subiculum participate in cognitive impairment remains largely elusive.METHODS In this study,we sought to label the subicular seizure-activated c-fos+ neu-rons with a special promoter with enhanced synaptic activity-responsive element E-SARE in the subiculum,combined with chemogenetics and designer receptors exclusively activated by designer drugs(DREADDs),Ca2+ fiber photometry approaches,and behavioral tasks,to reveal the role of these neurons in cognitive impairment in epilepsy.RESULTS We found that chemogenetic inhibi-tion of subicular seizure-tagged c-fos+ neurons(mainly CaMK Ⅱ α+ glutamatergic neurons)alleviates seizure generalization and improves cognitive performance in the hippocampal CA3 kindling TLE model.While inhibition of seizure-labeled c-fos+ GABAergic interneuron shows no effect on seizure and cognition.As a comparison,che-mogenetic inhibition of the whole subicular CaMK Ⅱ α+ neuron impairs cognitive function in na?ve mice in basal condition.Notably,inhibition of subicular seizure-tagged c-fos+ neurons enhances the recruitment of cognition-responsive c-fos+ neurons via increasing neural excitability during cognition tasks.CONCLUSION Our results dem-onstrate that subicular seizure-activated c-fos+ neurons contribute to cognitive impairment in TLE,suggesting sei-zure-tagged c-fos+ neurons as the potential therapeutic target to alleviate cognitive impairment in TLE.