1.The effects of chemokines in ocular neovascular diseases
Chinese Journal of Experimental Ophthalmology 2013;(5):517-520
Chemokine is a family of small chemotactic cytokines.Chemokine mainly locates in neutrophile granulocyte,monocyte,macrophage,T lymphocyte and B lymphocyte,and some cells and immunoactive cells also secret cytokines.Researches showed that chemokine plays important roles in many patho-physiological activities,such as inflammatory response,infection procedure,wound healing,regulation of immunity,angiogenesis,invasion and metastases of cancer,etc..Some chemokine factors,such as hyperglycemia,hypoxia,oxidative stress,stimulate the ocular histiocytes to up-regulate the expressions of CXC chemokines and CC chemokines,which participate in the development of choroidal neovascularization and ocular neovascular diseases.This article reviews current progress in chemokine and the relationship between chemokines and ocular neovascular diseases,especially in diabetic retinopathy (DR) and age-related macular degeneration (AMD) etc..
2.Cloning and expression analysis of 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase gene in Tripterygium wilfordii.
Yu-ru TONG ; Ping SU ; Meng ZHANG ; Yu-jun ZHAO ; Xiu-juan WANG ; Wei GAO ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(22):4378-4383
To clone the 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (TwMCT) full length cDNA from Tripterygium wilfordii, the specific primers were designed according to the transcriptome data and the LCPCR were carried out. After a series of bioinformatics analysis on the TwMCT, the MeJA induced expression content were investigated by real-time fluorescence quantification polymerase chain reaction (RT-qPCR). The result showed that the full of TwMCTcDNA was 1 318 bp nucleotides encoding 311 amino acids. The molecular weight of the deduced TwMCT protein was about 34.14 kDa and the theoretical isoelectric point was 8.65. Result of the RT-qPCR analysis indicated that the content of TwMCT mRNA expression in T. wilfordii suspension cell was rising after treating with MeJA and reached the maximum in 24 h. Cloning and analyzing TwMCT gene from T. wilfordii provided gene element for studying the function and expression regulation of secondary metabolites.
Amino Acid Sequence
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Cloning, Molecular
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Erythritol
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analogs & derivatives
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metabolism
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Gene Expression Regulation, Plant
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Molecular Sequence Data
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Nucleotidyltransferases
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chemistry
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genetics
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metabolism
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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metabolism
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Protein Structure, Secondary
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Sequence Alignment
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Sugar Phosphates
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metabolism
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Tripterygium
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chemistry
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enzymology
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genetics
3.Clinical significance of detecting CXC chemotatic factor in early diabetic retinopathy
Hong, ZHU ; Hai-lin, HU ; Meng-ru, SU ; Yao-chun, ZHU ; Wen-qiu, WANG ; Cai-hong, SHI ; Xiao-dong, SUN
Chinese Journal of Experimental Ophthalmology 2012;30(2):146-149
BackgroundDiabetic retinopathy (DR) is the result of the cytokine network disorders,the imbalance of angiogenic factor and vascular inhibitory factor is the start factor.ObjectiveTo analyze the levels of CXC chemotatic factors of type 2 diabetes mellitus patients,evaluate the clinical application value of them in different clinical types of DR using receiver operating characteristic (ROC)analysis and to approach the new way of individualized treatment.Methods This was a prospective research.The gold standard was ophthalmolscope and fundus fluorescein angiography.The levels of CXC chemotatic factors and multiplicaiton factors were measured in 96 cases with type 2 diabetes mellitus (66 cases with retinopathy and 30 cases without retinopathy as control).The assessment tasks were performed for these index and courses of DR with ROC curve.Results The expression of age,course of disease has significant difference in different courses of DR ( F =8.507,P =0.001 ; F =28.143,P =0.000).Compared with the control group,the expression of growth-related oncogene-α ( GROα ) ( t =- 2.172,P =0.035,AUC =0.625 ),whole blood viscosity 200 ( t =- 3.724,P =0.001,AUC =0.904 ) and neutrophilic leukocyte (t=-2.562,P =0.013,AUC =0.577 ) has significant difference in the group of mild NPDR.Compared with the control group,the expression of interferon-γ-inducible protein 10 ( IP-10 ) ( t =-3.591,P =0.001,AUC =0.592 ),platelet derivation growth factor-BB ( PDGF-BB ) ( t =- 3.233,P =0.003,AUC =0.735 ),vascular endothelial growth factor(VEGF) ( t =- 3.617,P =0.001,AUC =0.776 ),C peptide ( t =- 3.366,P =0.002,AUC =0.962 ),leukocyte ( t=-3.201,P =0.003,AUC =0.852) and neutrophilic leukocyte(t =-4.201,P=0.000,AUC =0.852) has significant difference in the group of moderate and severe NPDR.ConclusionsCXC chemotatic factors may act as reactivator in the pathogenesis of DR,GROα and IP-10 may be useful for clinical monitoring of the severity of DR,and evaluating the imbalance state of chemotatic factors maybe a new approach to clinical monitoring and prognosis of DR.
4.Cloning and bioinformatics analysis of geranylgeranyl diphosphate synthase gene of Tripterygium wilfordii.
Meng ZHANG ; Ping SU ; Yu-jia LIU ; Yu-ru TONG ; Yu-jun ZHAO ; Wei GAO ; Xiu-juan WANG ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(6):1066-1070
A full-length cDNA of GGPPS gene from Tripterygium wilfordii suspension cells was obtained by use of RACE strategy (GeneBank: KM978333), and then analyzed by bioinformatics approaches. TwGGPPS cDNA has 1857 nucleotides and an open reading frame (ORF) encoding a protein of 514 amino acid residues. The deduced protein has isoelectric point (pI) of 7.85, a calculated molecular weight about 57.13 kD, 5 conserved domains and 2 functional domains. PSORT Prediction showed it was located at plasma membrane. Phylogenetic analysis demonstrated that TwGGPPS1 was similar to GGPPS from other species of plants. For the first time the cloning of geranylgeranyl diphosphate synthase gene from T. wilfordii was reported, it lays the foundation for further research of diterpenoids biosynthetic pathway.
Amino Acid Sequence
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Cloning, Molecular
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Farnesyltranstransferase
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chemistry
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genetics
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metabolism
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Molecular Sequence Data
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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metabolism
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Sequence Alignment
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Sequence Homology, Amino Acid
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Tripterygium
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chemistry
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enzymology
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genetics
5.Cloning and expression analysis of 4- (cytidine-5-diphospho) -2-C-methyl-D-erythritol kinase gene in Tripterygium wilfordii.
Yu-ru TONG ; Ping SU ; Yu-jun ZHAO ; Meng ZHANG ; Xiu-juan WANG ; Tian-yuan HU ; Wei GAO ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(21):4165-4170
4-(Cytidine-5-diphospho) -2-C-methyl-D-erythritol kinase is a key enzyme in the biosynthesis pathway of terpenoids. According to the transcriptome database, the specific primers were designed and used in PCR. The bioinformatic analysis of the sequenced TwCMK gene was performed in several bioinformatics software. The Real-time fluorescence quantification polymerase chain reaction (RT-qPCR) were used to detect the expression levels of TwCMK from T. wilfordii after elicitor MeJA supplied. The results showed that the full length of TwCMK cDNA was 1 732 bp encoding 387 amino acids. The theoretical isoelectric point of the putative TwCMK protein was 5.79 and the molecular weight was about 42.85 kDa. MeJA stimulated the rising of TwCMK expression in suspension cell and signally impacted at 24 h. The research provides a basis for further study on the regulation of terpenoid secondary metabolism and biological synthesis.
Amino Acid Sequence
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Cloning, Molecular
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Computational Biology
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Gene Expression Regulation, Plant
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Models, Molecular
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Molecular Sequence Data
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Phosphotransferases (Alcohol Group Acceptor)
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chemistry
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genetics
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metabolism
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Phylogeny
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Plant Proteins
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chemistry
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genetics
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metabolism
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Sequence Alignment
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Tripterygium
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chemistry
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enzymology
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genetics
6.An elevated pretreatment serum globulin level predicts a poor prognosis of nasopharyngeal carcinoma.
Li-Ting ZHONG ; Hui WANG ; Huan-Qing LIANG ; Meng-Ru SU ; Cheng-Dong LIU ; De-Hua WU
Journal of Southern Medical University 2016;36(2):151-156
OBJECTIVETo investigate the value of serum globulin levels before treatment in predicting the prognosis of patients with nasopharyngeal carcinoma (NPC).
METHODSA total of 127 patients with non-disseminated NPC were recruited between January, 2009 and December, 2013 at Nanfang Hospital. The pretreatment serum globulin levels were analyzed with the receiver-operating characteristic (ROC) curve analysis to select the cut-off point for low and high pretreatment serum globulin levels. Kaplan-Meier and multivariable analyses were used to evaluate the predictive value of serum globulin levels.
RESULTSThe ROC curve analysis determined 30.05 g/L as the optimal cut-off value for pretreatment serum globulin level, which was significantly associated with gender (P=0.024) and N stage (P=0.016). Kaplan-Meier analysis showed that a high pretreatment serum globulin level (>30.05 g/L) significantly predicted poor progression-free survival (P=0.019), overall survival (P=0.034) and distant metastasis-free survival (P=0.049); multivariate analysis identified pretreatment serum globulin level as an independent prognostic factor for progression-free survival (HR=2.344, P=0.031).
CONCLUSIONPretreatment serum globulin level may serve as a valuable marker to predict the prognosis of patients with NPC.
Carcinoma ; Disease-Free Survival ; Humans ; Kaplan-Meier Estimate ; Multivariate Analysis ; Nasopharyngeal Neoplasms ; blood ; diagnosis ; Prognosis ; ROC Curve ; Serum Globulins ; analysis
7.Study on genetic polymorphisms of CYP2F1 gene in Guangdong population of China.
Ju-hong JIANG ; Zhi LI ; Guang SU ; Wei-hua JIA ; Ru-hua ZHANG ; Xing-juan YU ; Meng ZHANG ; Jian-ming WEN ; Yi-xin ZENG
Chinese Journal of Medical Genetics 2006;23(4):383-387
OBJECTIVETo investigate the genetic polymorphism of CYP2F1 gene, a member of CYP450 gene family in the healthy population and the patients with nasopharyngeal carcinoma (NPC) of Guangdong province, and furthermore analyze the relationship between CYP2F1 genetic polymorphism and the risk of developing NPC.
METHODSBy direct gene sequencing, all of 10 exons of CYP2F1 gene were detected in 40 peripheral blood specimens of patients with primary NPC. For the genetic polymorphism with high allelic frequency, mismatch PCR-RFLP technique was developed to identify the different frequency between 368 NPC cases and 344 cancer-free controls.
RESULTSThere were totally 35 SNPs identified in all of 10 exons and exon-intron junctions of CYP2F1 gene from 40 NPC patients, which included 10 missense mutations and 1 frame shift mutation. The most important mutation was C insertion located in 15-16 bp, which caused the frame shift. The allelic frequency of C insertion was 25%. However, there was no significant difference found between 368 NPC cases and 344 controls in allelic frequency of 15-16 bp C insertion mutation (P>0.05).
CONCLUSIONA lot of genetic polymorphism of CYP2F1 gene is found in Guangdong population of China. However, no single genetic polymorphism associated with the individual susceptibility to NPC can be identified. The cooperated operations with multiple genetic polymorphisms of one or more genes may be critical factors contributing to the development and progression of NPC.
Asian Continental Ancestry Group ; genetics ; Base Sequence ; China ; Cytochrome P-450 Enzyme System ; genetics ; Cytochrome P450 Family 2 ; Gene Frequency ; Genetic Predisposition to Disease ; genetics ; Humans ; Nasopharyngeal Neoplasms ; genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA
8.Investigation on compliance of forbidden depasturing livestock on marshland with Oncomelania snails in schistosomiasis endemic areas
Chunli CAO ; Jing WANG ; Ziping BAO ; Hongqing ZHU ; Shunxiang CAI ; Yiyi LI ; Dong LI ; Jiachang HE ; Leping SUN ; Xianhong MENG ; Bo ZHONG ; Xiguang FENG ; Zhengming SU ; Jun LI ; Xiaonan GU ; Hao WANG ; Weiwei RU ; Weisheng JIANG ; Shizhu LI ; Qiang WANG ; Xiaonong ZHOU ; Jiagang GUO ; Gengming ZHAO
Chinese Journal of Schistosomiasis Control 2009;21(6):482-485
Objective To study the compliance of forbidden depasturing livestock on the marshland with Oncomelania snails in schistosomiasis endemic areas. Methods According to 3 levels of human infection rates as > 10% ,5%-10% and <5% , 2 204 residents selected randomly from the schistosomiasis endemic villages were sampled with the stratified cluster sampling method in Hunan, Hubei, Jiangxi, Anhui, Jiangsu, Sichuan and Yunnan provinces, and investigated by questionnaire. The contents of the questionnaire included the recognition and implementation of forbidden depasturing livestock on marshland with Oncomelania snails and breeding livestock in bam. Results A total of 78.4% residents agreed forbidden depasturing livestock on marshland with snails, but 3. 7% residents disagreed it. A total of 83. 9% residents considered the relationship between breeding livestock in bam and schistosomiasis control, but 3. 1% residents thought that it was no relationship. The main reasons of depasturing livestock on marshland with Oncomelania snails were the high cost of breeding livestock in bam (36. 2% ) , unaccustomed (26.4% ) and no room for breeding livestock in bam (25.4% ). Conclusion Forbidden depasturing livestock on the marshland with Oncomelania snails should be strengthened according to the local economic, nature environment, agriculture, residents'culture degree and agriculture habit.
9.Soil Physical and Chemical Properties, Microorganisms and Metabolites in Different Culture Environments of Gastrodia elata
Pei WANG ; Guang-yun MENG ; Ru-zhi MAO ; Kuan YANG ; Zhao-hui SU ; Zhong-qiao WANG ; Shun-qiang YANG ; Hong-ping HUANG ; Xia-hong HE
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(14):164-174
Objective:To study the soil physical and chemical properties, microorganisms, and metabolites in different culture environments of
10.Research in technical regulations for production of pollution-free Chinese herbal medicines.
Lin-Lin DONG ; Li-Li SU ; Guang-Fei WEI ; Meng-Zhi LI ; Ya-Ru XU ; Huan-Huan WANG ; Shi-Lin CHEN
China Journal of Chinese Materia Medica 2018;43(15):3070-3079
Aimed to solve the issues of pesticide residue, heavy metal contents and harmful elements in the productive process of Chinese herbal medicines, the research team built the technical regulations for production of pollution-free Chinese herbal medicines. This regulation included the environment of production area, the process of production, quality of products etc., which were the key steps controlled the quality of Chinese herbal medicines. The environment of production area was selected according to the ecological factors which were stipulated by Ecological Suitability Regionalization of Chinese herbal medicines (second edition). The quality of air should be attain the one or two levels of GB/T3095-2012 standard values. The cultivation soils should reach to the one or two levels of GB15618 and NY/T391 standard values. The quality of irrigation water should accord with the stipulation of GB5084-2005. Aimed to the production of Chinese herbal medicines, disease-resistant and superior varieties which were suitable to the local stations should be selected, and the breeding of superior seeds and seedlings should be strengthened. Additionally, rational fertilizer application of pollution-free Chinese herbal medicines should be conformed to the principles, requirements, and the kinds of fertilizers allowed or limited for use, which were stipulated by the standard of DB13/T454. Furthermore, the plant protection policy of priority to prevention and synthetical prevention should be followed; improving ecological environment and strengthening cultivation management should be served as the basics. Agricultural measures, and biological and physical control strategies should be preferred to use; and high toxicity, residue pesticide and its mixture should be inhibited; the use of chemical pesticides should be minimized and then to decrease contamination and residue. Additionally, the quality of products should be reached to the standard of pollution-free Chinese herbal medicines; high toxicity and detection rate of pesticide residues and the contents of heavy metal and harmful elements (e.g. plumbum, cadmium, mercury, arsenic and cuprum) should accord with the common criteria of pollution-free Chinese herbal medicines. Application of technical regulations for production of pollution-free Chinese herbal medicines guarantees significantly social, economic and ecological benefits.