1.Effects of midazolam on macroscopic voltage-gated potassium currents in rat sympathetic neurons
Qinghong MAO ; Xinliang ZHUANG ; Meng CHEN ; Jun TANG ; Guohui XU ;
Chinese Journal of Clinical Pharmacology and Therapeutics 2000;0(03):-
AIM: To explore the influence of different concentration midazolam on the macroscopic voltage gated potassium currents and to discuss the relationship between potassium currents and inhibitory effect of clinical relevant concentration midazolam on sympathetic nervous system. METHODS: Superior sympathetic ganglion neurons were dissociated enzymatically from 7 to10 day old rat. Experiments were performed about 5 h after plating at room temperature (20- 24 ℃ ). Appropriate solution was chosen to separate the K + current from the other transmembrane currents. 1 ?mol?L -1 TTX was applied to the extracelluar solution to block the Na + current. Midazolam was also resolved in extracelluar solution to get various concentration ( 0.1 , 0.3 ,3,10,50,100 ?mol?L -1 ). Currents were recorded with the patch clamp technique in whole cell configuration using glass electrodes with a tip resistance of 2- 4 M . Potassium currents were evoked by test pulse from -100 mV to +30 mV with holding potential -80mV. Data were analyzed using Clampfit 6.0 and Oringih 5.0 software. Whole cell current records were corrected for leakage and capacitance by using the P/5 protocol. RESULTS: Midazolam dose dependently inhibited the whole cell potassium currents. Clinical relevant concentration midazolam ( 0.3 ?mol?L -1 ) only reduced the peak currents by 3.89 %(P= 0.88 ). The concentration required to produce 50% current inhibition(IC 50 ) was 76.065 ?mol?L -1 . CONCLUSION: Midazolam inhibits the whole cell potassium current significantly and dose dependently, but clinical relevant concentration midazolam has minor effect on the potassium currents, indicating that the inhibitory effect of midazolam on potassium current is not related to the suppression of activity of sympathetic system.
2.Complications caused by EN through nasogastric tube after stomach cancer surgery
Xuesheng FAN ; Maoming XIONG ; Xiangling MENG ; Jun FANG ; Dawei TANG ; Dezhi WANG
International Journal of Surgery 2009;36(3):157-160
Objective To evaluate the causation and management of complications caused by enteral nutrition (EN)after stomach cancer surgery.Methods The clinical data of 195 gastric cancer patients which used EN after surgery were analyzed retrospectively from September 2002 to April 2008 in our hospital.Results Among the 195 patients,29(14.87%)developed abdominal distention,17(8.71%)diarrhoea,Six(3.07%)metabolic complica tions,three anastomotie leakage,1 patient with colon perforation.Conclusion These complications caused by EN after stomach cancer surgery associated with the stress of surgery,the speed and concentration of nutrition infusion, patients'metabolic conditions and other related factors.
3.Study on the characteristics of temporal distribution and the epidemic trend of autumn-winter type scrub typhus under time series analysis
Lei DING ; Shu-Jun DING ; Meng ZHANG ; Xian-Jun WANG ; Zhong LI ; Zhong-Tang ZHAO
Chinese Journal of Epidemiology 2012;33(7):698-701
Objective To explore the characteristics of temporal distribution and epidemic trend of autumn-winter type scrub typhus using the time series analysis.Methods Based on the data of scrub typhus collected from Shandong Diseases Reporting Information System from 2006 to 2011,both spectral analysis and moving average analysis were used to analyze the annual data of scrub typhus while scrub typhus incidence in 2012-2014 was forecasted.Seasonal decomposition analysis was applied to analyze the monthly data from January of 2006 to October of 2011,followed by Autoregressive Integrated Moving Average Model (ARIMA) which was constructed to forecast case number in November and December of 2011 and compared to the actual incidence.Results The results of spectral analysis showed that the prevalence of autumn-winter type scrub typhus had a feature of ‘3-year-periodicity’.A long-term up-trend was confirmed by method of moving average analysis,with annually case numbers of 310,337 and another number of 366 forecasted for 2012 to 2014,respectively,with the annual increase rate as 9% per-year.Data from analysis of monthly data of scrub typhus showed that through multiple seasonal decomposition analysis,the results indicated that the prevalence of this disease possessed a typical autumn-winter type.The seasonality indexes for scrub typhus in October and November were 8.454 and 2.230,respectively,while others were less than 1.000.The ARIMA (0,1,1 ) (0,1,0)12 model of ( 1 -B) ( 1 -B12)X,=( 1 -0.811B)u,that was used to forecast the prevalence of autumn-winter type scrub typhus and was constructed with the residual error of 16 lags as white noise.The Box-Ljung test statistic for the model was 3.116,giving a P value of 0.999.The model fitted the data well.Good accordance was achieved between the observed values and the forecasted values of scrub typhus in November and December of 2011 which was produced by the ARIMA model,and all observed values were within the forecasted 95% CI.Conclusion The prevalence of autumn-winter type scrub typhus showed a 3-year-periodicity,with a long-term up-trend,and the case numbers of 2012 to 2014 were forecasted,rising on the end with an increasing rate of 9% per year,which occurred seasonally with October as the peak time in every year.The ARIMA (0,1,1 ) (0,1,0) 12 model seemed to be quite appropriate in predicting the autumn-winter type scrub typhus.
4.Sigma rectum pouch for urinary diversion(Report of 18 cases)
Pei-Jing HOU ; Guang-Bo FU ; Yun-Yan WANG ; Hai-Jun ZHUANG ; Jun-Song MENG ; Peng TANG ;
Cancer Research and Clinic 2006;0(10):-
Objective To assess the continent diversion results of sigma rectum pouch after radical cystectomy. Methods The reconstruction of bladder with sigmoid was modified for treatment of 18 cases of bladder tumor.The intestine was incised over a length of 20~24 cm with the junction of sigmoid colon and rectum as the midpoint so as to create a low pressure reservoir for urine and side-to-side anastomosis was performed on the posterior borders of the rectosigmoid wall.Submucosal tunnel modified technique was em- ployed in antireflux urethral implantation,Urination has been controlled by anal sphincter.Results About 80 minutes was spent to finish a new low pressure pouch after radical cystectomy.Among 18 patients with this op- eration,the controlled emiction were good after pull out the anal duct and"J"stent in 1 week to 2 months.Af- ter 2 months,the times of urination is stable,4~5 times in daytime and 1~3 times during nighttime.Two pa- tients had nocturnal enuresis and the symptom vanished after 2 months. One patient had adhesive ileus, two patients had hyperchloremia acidosis and kaliopenia,one patient had urethral stump cancer.There is no com- plication as anastomotic block,renal function lesion and severe upper urinary tract infection. Conclusion This operative method was easy,emiction control was well,and with higher quality of life for patients.It is al- so a better alternative diversion procedure that would be easily accepted.
5.Progress in research on the susceptibility of tuberculosis
meng Meng SUN ; Jun TANG ; Chuan QIN ; jun Ling ZHAN
Chinese Journal of Comparative Medicine 2017;27(11):106-112
Tuberculosis(TB),a chronic infectious disease caused by Mycobacterium tuberculosis(MTB), is the outcome of the interaction between polygenes and environmental factors,which has been shown in lots of genetic studies. In recent years,the research of TB susceptibility genes and their single nucleotide polymorphism(SNP)loci has become a hot topic,but genes and SNPs exactly associated with TB susceptibility are rarely reported. What's more,some susceptibility genes are different among races, populations and geographical distributions, resulting in inconsistency, or even contrary conclusions drawn from local studies of different populations or areas. In order to obtain susceptibility genes that are widely applicable and exactly associated with TB,it is necessary to screen the genes preliminarily in the laboratory animals with gene diversity, and thereafter, verify those genes in animals or humans. The Collabrative Cross(CC)mice have the advantages of diverse and clear genetic background,which can provide a new tool for the research of TB susceptibility. This review summarizes the advances in the studies of TB susceptibility genes and their SNPs, and looking forward to the application of CC mice in these studies and its significance.
6.Comparative study of dissolved matter of glycyrrhizic acid before and after supermicro-pulverization of Radix Glycyrrhizae.
Zhi-meng LI ; Yue-sheng WANG ; Xiao-ming LI ; Han YAN ; Jun ZHANG ; Xiao-jun TANG ; Xin LI
China Journal of Chinese Materia Medica 2003;28(11):1030-1033
OBJECTIVETo investigate various activities of dissolved matter of glycyrrhizic acid of Radix Glycyrrhizae and the powder by supermicro-pulverization.
METHODThe contents of glycyrrhizic acid in different samples were tested.
RESULTThe dissolved matter of glycyrrhizic acid was greatly increased by supermicro-pulverization. The more time used for grinding, the smaller the size of the powder, and the easier the glycyrrhizic acid would be dissolved.
CONCLUSIONSupermicro-pulverization is helpful to the dissolved matter of glycyrrhizic acid of Radix Glycyrrhizae, and the size of powder exerts great influence on dissolved matter of glycyrrhizic acid.
Drug Combinations ; Drugs, Chinese Herbal ; chemistry ; Glycyrrhiza ; chemistry ; Glycyrrhizic Acid ; analysis ; Particle Size ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Powders ; Solubility
8.Investigation on the anti-tumor effect and mechanisms of the glycosyl phosphatidylinositol modified routine IL-21 tumor vaccine
Fengshu ZHAO ; Lili CHU ; Jun DOU ; Quan TANG ; Yongfang WANG ; Yun WU ; Minggang CAO ; Meng PAN ; Ning GU
Chinese Journal of Microbiology and Immunology 2008;28(9):822-827
Objective To construct the murine IL-21 (mIL-21) tumor vaccine modified by glyco-syl phosphafidylinositol(GPI), and to evaluate its anti-tumor effect and mechanisms. Methods The IL-21-GPI gene was acquired by overlap PCR and inserted into PeDNA3.1. The recombinant plnsmid pcDNA3.1/ IL-21-GPI was transformed into cell B16F10, and the expression of mIL-21 on cell membrane was deter-mined by cell indirect immumofluorescence and flow cytometry (FCM). The bioactivity of mIL-21 was iden-tiffed according to its effects on the proliferation of mouse spleen cells. The anti-tumor effect was evaluated depending on the tumor size and the survival of tumor-beating mice after the tumor vaccine was inoculated into C57BL/6 mice. And the activity of cell-mediated immunity in immunized mice was detected at the same time. Results The recombinant plasmid pcDNA3.1/IL-21-GPI was correctly constructed, which could ex-press mIL-21 binding the membrane with good bioactivity. The vaccine had good anti-tumor effect, and the cell-mediated immunity had been improved in immunized mice. Conclusion The GPI modified mIL-21 tumor vaccine with anti-tumor activity was constructed successfully, which provided a good foundation for studying anti-tumor immunity and therapy in future.
9.Regulatory Effect of Qushi Huayu Recipe on Gene Expression Profiles of Fatty Liver Rats.
Qin FENG ; Ya-jun TANG ; Xiao-fei LI ; Sheng-xi MENG ; Jing-hua PENG ; Yu ZHAO ; Yi-yang HU
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(2):203-209
OBJECTIVETo observe the intervention and mechanism of Qushi Huayu Recipe (QHR) on gene expression profiles in high lipid diet induced fatty liver rats.
METHODSFatty liver model was prepared in 20 male SD rats using single high fat diet (88% common forage +2% cholesterol +10% lard). Four weeks after modeling they were divided into the model group and the QHR group according to random digit table, 10 in each group. QHR (at 0. 93 g crude drug/100 g body weight) and distilled water was respectively to rats in the QHR group and the model group by gastrogavage while modeling, once per day. Meanwhile, 10 SD male rats were recruited in a normal group, administered with equal volume of distilled water by gastrogavage. At the end of week 8 all rats were sacrificed, and blood and livers were collected for subsequent analysis. Contents of liver triglyceride (TG) and free fatty acid (FFA) , activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected using biochemical assay. Pathological changes of liver tissue were observed using H&E and oil red O stain. Liver gene expressions were detected by Affymetrix gene expression profiles. Differentially expressed genes were compared between the QHR group and the model group, functions of differentially expressed genes and signal pathways involved analyzed. Ten differentially expressed genes involved in glycolipid metabolism with fold change more than 2 were selected for verification by real-time PCR.
RESULTS(1) Compared with the normal group, contents of liver TG and FFA, and serum activities of ALT and AST obviously increased in the model group (P <0. 01). Compared with the model group, contents of liver TG and FFA, and activities of ALT and AST obviously decreased in the QHR group (P <0. 05, P <0. 01). QHR could reduce high fat induced fatty degeneration of liver cells , alleviate inflammation, and improve pathological changes of liver tissue. (2) Compared with the model group, there were 80 differentially expressed genes (with fold change > 2, P < 0.05) with clear functions and appointed gene names, including 44 up-regulated and 36 down-regulated genes. Eighty genes were involved in 27 signal pathways with statistical difference, including glycerolipid metabolism, adipocytokine signaling pathway, insulin signal pathway, drug metabolism signal pathway, etc (P < 0.05). (3) RT-PCR results of 10 glycolipids metabolism regulating genes such as Gk, Scd1, Gpat2, G6pc, Irs1, and so on showed that all RT-PCR genes were completely coincide with up-regulated or down-regulated tendency in results of gene chips. 80% genes had approximate fold change.
CONCLUSIONQHR could regulate gene expressions related to fat metabolism, carbohydrate metabolism, anti-lipid peroxidation, and drug metabolism in high fat diet induced fatty liver rats, and its comprehensive pharmacological actions could be manifested.
Alanine Transaminase ; metabolism ; Animals ; Aspartate Aminotransferases ; metabolism ; Carbohydrate Metabolism ; Diet, High-Fat ; Drugs, Chinese Herbal ; pharmacology ; Fatty Acids, Nonesterified ; metabolism ; Fatty Liver ; metabolism ; Lipid Metabolism ; Lipid Peroxidation ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Transcriptome ; drug effects ; Triglycerides ; metabolism
10.Prepartation of Gelsolin-targeted ultrasound contrast agent and experiment in vitro
Haocheng QIN ; Jun WU ; Meng ZHOU ; Yuhong ZHANG ; Yu SONG ; Jieming LI ; Xiaona WEN ; Jianwu TANG ; Haitao RAN
Chinese Journal of Medical Imaging Technology 2017;33(6):826-831
Objective To prepare a kind of Gelsolin-targeted ultrasound contrast agent (GSN-PLGA) and to explore its targeting and imaging effection in vitro.Methods The high molecular PLGA-COOH ultrasound contrast agents were prepared by a modified double emulsion technique and then conjugated with Gelsolin monoclonal antibody by carbodiimide technique.The physical property of contrast agent was determined.And the connectivity condition of ultrasound contrast agent with Gelsolin monoclonal antibody was estimated.The targeting ability and the effect of enhancing ultrasound imaging in vitro were explored.Results The average diameter of GSN-PLGA was (575.67 ± 4.71) nm.The potential was (-11.46±1.19) mV.And the binding rate of Gelsolin monoclonal antibody was 96.93%.In vitro experimentshowed more GSN-PLGA could be intaked by Hca-F cells and the ultrasound imaging cloud be enhanced greatly.Conclusion The GSN-PLGA nanoparticle can bind to Hca-F cells specifically and can enhance the ultrasound imaging greatly.