Objective:To study B cells proliferation and activation induced by tumor derived-autophagosomes ( TDA). Methods:Splenocytes of mice were incubated with 10μg/ml TDAs in vitro ( with the 10μg/ml whole cell lysate as the control).At the 3rd day,the expression of MHCⅠ,Ⅱand co-stimulate molecules including CD86,CD40 were detected by flow cytometry,and B cells proliferation was detected at the 5th day.B cells were selected from spleen of mice using anti-CD43 dynabeads,and incubated with 10μg/ml TDA in vitro ( with the same concentrated whole tumor cell lysate as the control).At the 7th day,IgM in the supernatant were tested by ELISA.Results:When compared with whole cell lysate stimulated B cells,TDA efficently stimulated B cells division in vitro ( TDA group:28.6%, Whole cell lysate group:4.4%) , and significantly up-regulated the expression of MHC class Ⅰ,Ⅱ and co-stimulatory molecules (CD86 and CD40) on B cells,and enhanced the levels of total IgM secretion in vitro.Conclusion:Tumor-derived autophagosomes ( TDA) ,as a vacuole antigen vector, could stimulate B cells proliferation, activation, and increased IgM secretion in vitro.

Extract of Ground Beetle was found to affect rat rheology. when given orally to rats for 10 consecutive days, it resulted in the lowering of packed corpuscular volume, high and low whole blood shear viscosity, RBC aggregation and rigidity indices and sedimentation rate,but the constant of SR equation was raised and without effect on Plasma viscosity and fibrinogen content. These results suggested that the effect of ground beetle extract is by way of its action on RBC

Purpose To measure the nuclear morphological parameters of breast cancer to study the relationship between nuclear mor-phological parameters and ER, PR, HER-2 expression, and clinicopathologic features of breast carcinoma. Methods 388 cases of breast cancer specimens were collected and molecular classification was made according to ER, PR and HER-2 expression. the nucleus parameters were measured by image analysis software after HE staining. The difference among groups was statistically analysed, and follow-up was done by phone or by hospitalization. Results Among the 4 groups of breast cancer case, the differences of circle diame-ter, area and perimeter edges of the nucleus were statistically significant (P<0.05). Nuclear morphometric quantitation between ER+/PR+ patients and ER-/PR- patients was statistically significantly differentice (P<0.05). The majority of patients with ER-/PR- were histological gradeⅢand poor survival rate (P<0.05). The disease-free survival in Luminal A type was higher than that of Basal-like type (P<0.05), and its overall survival was higher than HER-2 over-expression (P<0. 05) and Basal-like type (P<0.05) . Conclusion The nucleus morphological quantitation in breast cancer is of significant difference, which has certain reference value in its molecular typing. The result of ER, PR and HER-2 expression, combined with nuclear morphology measurement, are meaningful to the treatment and assessment of prognosis.

OBJECTIVE:To evaluate the relative bioavailability of two products(CLMC 1 ,CLMC 2 )of Clarithromycin in man.METHODS:A single oral500mg dose of CLMC 1 or CLMC 2 was given to20healthy male volunteers in an open randomized crossover study.The plasma concentrations of CLMC 1 and CLMC 2 were measured by microbial assay.The pharmacokinetic parameters were calculated with3p97pharmacokinetic program and the bioequivalence was evaluated.RESULTS:The con?centration-time curves of CLMC 1 and CLMC 2 fitted to a two-compartent open model.C max were(2.23?0.83)?g/ml and(2.14?0.70)?g/ml;T max were(1.95?0.39)h,(1.78?0.41)h;AUC (0～T) were(9.50?2.52)(?g?h)/ml,(9.35?2.54)(?g?h)/ml respectively.The relative bioavailability of CLMC 1 was(101.60?9.35)%.CONCLUSION:The results of two and one-side t tests suggest that the CLMC 1 is bioequivalent with the CLMC 2 .

Objective: To observe the effect of ethanol extract of Crataegus Pinnatifida Bge. Var. major N. E. Br. from Hei-longjiang province on hemodynamics of acute myocardial ischemia caused by ligation and platelet aggregation induced by isoproterenol. Methods:After the model establishment of acute myocardial ischemia by ligation in rats, the systolic blood pressure ( SBP) ,diastolic blood pressure ( DBP ) , heart rate ( HR ) , left ventricular systolic pressure ( LVSP ) , left ventricular end-diastolic pressure ( LV-EDP) , left ventricular peak systolic ( +) and diastolic ( -) rate ( ± dp ·dt max-1 ) were recorded. Meanwhile, the acute myocar-dial ischemia model induced by isoproterenol was established. The platelet aggregation was measured by a platelet aggregation instru-ment. Results:The ethanol extract of Crataegus Pinnatifida Bge. Var. major N. E. Br. from Heilongjiang province could significantly increase the SBP, DBP, LVSP, +dp · dtmax-1 and HR, and reduce LVEDP,-dp·dtmax-1 in high dose group with statistical sig-nificance (P<0. 05). The medium dose group had small impact on the DBP, while showed statistically significant effect on the other hemodynamic indices. The low dose group had statistically significant effect on the SBP, LVSP and ± dp·dtmax-1 (P<0. 05), while showed no notable effects on the other indices. The results of aggregation rate in 1 min and 5 min after the determination and the maxi-mum one in the high and medium dose groups showed that the ethanol extract could significantly inhibit the platelet aggregation with sta-tistical significance (P<0. 05). Conclusion:The ethanol extract of Crataegus Pinnatifida Bge. Var. major N. E. Br. can improve the blood flow of acute myocardial ischemia in rats and reduce platelet aggregation after ischemia.

Aged ; Female ; Hearing Loss, Bilateral ; diagnosis ; etiology ; Humans ; Osteitis Deformans ; complications ; diagnosis

The application of cerebrovascular intervention brings the diagnosis and treatment of cerebrovascular disease into a brand-new domain. Many neurological physicians have become physicians of cerebrovascular intervention by training. In the big teaching hospitals carrying out cerebrovascular intervention, advanced physicians are the hard strength in the lot of cerebrovascular intervention. The training aiming at cerebrovascular intervention and comprehensive abilities affects both the training results and the therapeutic results of cerebrovascular intervention. Therefore, it is an important issue to explore how to consummate and standard the training methods of advanced physicians studying cerebrovascular intervention.

Objective:To analyze the experience of XU Peng-lin in using medicine in treating chronic gastritis,exploring the method for summarizing the experience of famous TCM practitioner.Methods:Professor Xu's 212 typical cases of chronic gastritis were collected,using related rules and point mutual information to analyze the prescription compatibility for the chronic gastritis.Results:Utilization of 12 kinds of drugs were over 30 percents,includinging Pinellia,dried ginger,mongolian milkvetch root,Medicinal Evodia Immature Fruit,and liquorice root,etc..The common compatibility rules were warming spleen-stomach to eliminat dampness,acridness and sweetness activating yang,sour-sweet herbs nourishing yin,pungency disperses while bitterness descends,application of both cold and heat,and combination of reinforcing and reducing.The basic prescriptions for chronic gastritis used by professor XU were Pinelliae Decoction for Purging Stomach-Fire,Zuojin Pill,Huangqi Jianzhong Decoction.Conclusion:Harmonizing yin and yang,reinforcing deficiency and reducing the excess,regulating the activities of qi,harmonizing zang and fu organs were the treatment for chronic gastritis by professor XU,which provided the methodological reference toTCM in treating the chronic gastritis.

The family functioning of stroke patients is lower and related to many factors. This paper reviews the recent study in family functioning and measurement tools; the impact factors in terms of family structure, family economic status, social support and family development stage and life events.