1.Relationship Study on Serum Levels of Procalcitonin and Pulmonary Function in Patients with Chronic Obstructive Pulmonary Disease
Jun-Min LI ; Xiao BAI ; Xi MENG ; Jiao TIAN
Journal of Modern Laboratory Medicine 2018;33(1):148-150
Objective To study the relationship between the serum levels of procalcitonin (PCT) and pulmonary function in patients with chronic obstructive pulmonary disease (COPD).Methods From January 2016 to May 2107,88 cases of patients with COPD and 100 cases of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) were chosen as the object of study,pulmonary function was measured in all patients,grouped according to the standard of pulmonary function classification,and detected the serum levels of PCT by immunoturbidimetry.Results The serum levels of PCT were not statistically different among the COPD patients (F=1.401,P>0.05).But among the AECOPD patients,the serum levels of PCT in the Ⅳ class of pulmonary function were significantly higher than that in the Ⅰ,Ⅱ class and Ⅲ class and this difference was statistically significant (F=9.128,P<0.05).Conclusion The serum levels of PCT had significant reference value for the judgement of the severity of pulmonary function impairment in AECOPD patients.
2.Study on activated protein C resistance and disordered coagulation in patients with myeloproliferative neoplasms.
Jie BAI ; Yang-ping XUE ; Xian-hui XU ; Lei ZHANG ; Meng-su TIAN ; Lin SHEN ; Hong-yun ZHANG ; Ren-chi YANG
Chinese Journal of Hematology 2011;32(3):187-190
OBJECTIVETo study the correlation of activated protein C (APC) resistance, coagulation factors and inhibitors abnormality and JAK2V617F mutation burden in patients with myeloproliferative neoplasms (MPN).
METHODSThe APC resistance was defined as the ratio of activated partial thromboplastin time (APTT) in the presence and absence of APC, i.e. APC sensitivity ratio (APCsr). Plasma protein C (PC), protein S (PS), prothrombin (FII), factor V (FV), factor VIII levels and CD11b expression on neutrophils were measured. The percentage of mutated JAK2V617F allele (V617F%) was evaluated by real time polymerase chain reaction (qRT-PCR).
RESULTSExpression of CD11b on neutrophils was significantly elevated in MPN patients compared with that of the control group. APCsr, PS and FV levels were reduced in patients with MPN. The APCsr level was decreased mainly in patients with thrombosis and JAK2V617F mutant burden higher than 75%. APCsr was not only positively correlated with PS levels but also inversely correlated with JAK2V617F allele burden in JAK2V617F mutant gene carriers.
CONCLUSIONThe neutrophil was activated and PS, FV level were reduced in MPN patients. The APCsr level was decreased and the occurrence of relatively acquired APC resistance was found in MPN patients with thrombosis. The APCsr is correlated with the PS level and JAK2V617F mutational furden.
Activated Protein C Resistance ; metabolism ; Adolescent ; Adult ; Aged ; Blood Coagulation ; Blood Coagulation Disorders ; Factor V ; metabolism ; Female ; Humans ; Male ; Middle Aged ; Myeloproliferative Disorders ; blood ; metabolism ; Protein S ; metabolism ; Young Adult
3.Preliminarily screening of serum characteristic markers in acute myeloid leukemia and clinical significance.
Ai-Li HE ; Ju BAI ; Chen HUANG ; Wang-Gang ZHANG ; Juan YANG ; Jian-Li WANG ; Xin MENG ; Wei TIAN
Journal of Experimental Hematology 2010;18(5):1132-1137
This study was purposed to preliminarily screen characteristic tumor markers of acute myeloid leukemia (AML) and to investigate the serum proteomics characteristics of patients with AML and their significance in pathogenesis. 14 patients with AML and 28 healthy controls were enrolled in this study. The serum protein components were captured by weak cation exchange nanometer magnetic beads, the protein mass-spectra of all samples were detected by Autoflex II matrix-assisted laser desorption/ionization time of flight mass spectrometer, and the detection data were analyzed by means of CliprotoolsTM2.2 software, then the differential protein molecules were screened and the diagnostic model was established. Sera of 7 AML patients and 14 healthy controls were selected to verify the established model by using blind test. The results indicated that about 69 protein peaks could be detected within the range of 0.7-10 kD in protein spectra of serum samples from AML patients and controls. Compared with healthy controls, there were 44 statistically differential expression peaks in AML group (p<0.0001). Among them, 10 protein peaks were upregulated protein peaks and 34 protein peaks were downregulated. Diagnostic model was established on the basis of Quick Classifier Algorithm (QC), and the three mass peaks had the strongest power for software to automatically distinguish AML group from control group. Mass charge ratios (m/z) were 3216.57, 4089.7, and 7762.87 respectively. Sensitivity was expected as 86.4% while 82.8% in this established model group. Category validation showed that this diagnostic model correctly identified all 6 cases out of AML and 12 cases out of 14 healthy controls. In cross validation, the model sensitivity and specificity both were 85.7%. It is concluded that the AML QC model is composed of three protein peaks, which can effectively distinguish AML patients from healthy controls. Owing to higher sensitivity and specificity, they may act as serum tumor markers of AML. Among the three proteins, the one with m/z 7762.87 is the platelet-derived protein chemokine (PF4) protein. This finding will probably provide significant experimental evidence for understanding pathogenesis, molecular type, prognosis and treatment effect of AML.
Adult
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Aged
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Biomarkers
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Biomarkers, Tumor
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blood
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Case-Control Studies
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Female
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Humans
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Leukemia, Myeloid, Acute
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blood
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diagnosis
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Male
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Middle Aged
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Prognosis
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Protein Array Analysis
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methods
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Proteomics
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Sensitivity and Specificity
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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methods
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Young Adult
4.Prevalence of vitiligo in China:an epidemiological survey in 6 provinces
Xiaoyan WANG ; Tinglin WANG ; Cheng ZHOU ; Yiwei SHEN ; Xiaolan DING ; Shan TIAN ; Ying LIU ; Guanghui PENG ; June ZHOU ; Shuqi XUE ; Renli WANG ; Ying TANG ; Xuemei MENG ; Guangde PEI ; Yunhua BAI ; Qing LIU ; Hang LI ; Juan DU ; Jianzhong ZHANG
Chinese Journal of Dermatology 2010;43(7):463-466
Objective To investigate the prevalence of vitiligo in China through a multi-center and larse-population epidemiological survey.Methods A community-based survey was conducted in 6 cities from 6 provinces.Cluster sampling method was used.Subjects were required to fulfill the self-report questionnaires and received physical examination by dermatologists.EpiData and SPSS11.5 were utilized for statistical analysis. Results Totally,19 974 patients participated in this study,and 17 345 valid questionnaires were retrieved with a return rate of 86.84%.Of them,122 were found to have vitiligo.The prevalence and standardized prevalence of vitiligo was 0.70% and 0.56% in all patients,0.95% (75) and 0.69% in male patients and 0.50% (47) and O.45% in female patients.respectively.A significant elevation was observed in the prevalence of vitiligo in males than in females (P<0.01).The prevalence of vitiligo was increased with age and peaked in patients aging from 60 to 69 years and those over 70 years.The age at onset of vitiligo varied from 0 to 19 years in 21.85% of these patients,from 20 to 49 years in 47.05%.The most connnon type was focal vitiligo,which accounted for 36.06%,while the rarest type wag segmental type (2.46%).The pesitivity rate of family history of vitiligo was 9.84% in patients and 1.31% in community population (P<0.01).About 31.97% of the patients complained of negative influence of vitiligo on quality of life.Conclusions The standardized prevalence of vitiligo is 0.56%in 6 provinces from China.Males seem to have a higher prevalence of vifiligo than females.
5.Protective effects of zingiber officinalis extract on the lens of diabetic rats
Meng-Tian BAI ; Gang-Jin KANG ; Man-Hua XU ; Mao-Jiao LI ; Zheng-Hong PENG ; Jian WU ; Yun LI
Recent Advances in Ophthalmology 2018;38(5):425-429,433
Objective To observe the protective mechanisms of zingiber officinalis extract on the lens of diabetic rats induced by streptozotocin (STZ).Methods Totally 60 SD rats were randomly divided into A group (normal control rats),B group (diabetic rats),C1 group (DM +50 mg · kg-1 ginger gavage),C2 group (DM + 100 mg ·kg-1 ginger gavage) and C3 group (DM + 300 mg · kg-1 ginger gavage).Each group had 12 rats.And rats in A group received intraperitoneal injection of the same volume of normal saline,while the other groups were intraperitoneally injected with 65 mg · kg-1streptozotocin (STZ).When the animal model was successfully established,both A group and B group were administered orally with normal saline,and the C1,C2 and C3 groups were administered orally with ginger rhizome extract.The changes in blood glucose and lens were observed every week.The rats were sacrificed in succession at 4 week,8 week,12 week,and the lens was removed immediately.The content of aldose reductase (AR) was detected by ELISA,and the expression of glycosylation end products (AGEs) was detected by Western blot.The superoxide dismutase (SOS) activity and malondialdehyde (MDA) content were also detected.Fluorescent Tunel staining was used to detect the apoptosis of the lens epithelial cells.And finally,scanning electron microscopy was used to observe the ultrastructural changes in the lens.Results The activity of SOD in the lens of diabetic rats showed a decreasing trend with statistical significance (all P < 0.05).Changes in the content of MDA in the lens of rats in B,C1,C2 group were statistically significant in 4,8 and 12 weeks after successful modeling (all P < 0.05),but no significant difference in A group and C3 group (both P > 0.05).The persistent increase of AR in group B (P =0.003).The content of AR of the lens in C1,C2,C3 group showed a decreasing trend,and the differences were statistically significant (all P < 0.05).There were significant differences in the expression of AGEs in C1,C2 and C3 group (all P < 0.05).The degree of cortical fiber destruction in group B was progressively aggravated,but the degree of cortical destruction in C1,C2 and C3 group decreased with the increase of ginger gavage concentration through the scanning electron microscopy.It was observed that there was no significant difference in the apoptotic rate of LECs in A group (P =0.191),but the apoptosis of LECs in the rest group showed a rising trend,and the differences were statistically significant (all P < 0.05).Conclusion The effects of ginger gavage extract can delay the opacification of lens and slow down the diabetic development in rats with a dose-independence manner.Ginger gavage extract may play a protective role on the lens of diabetic rats by inhibiting the activity of AR,oxidative stress and the production of AGEs as well as suppress the apoptosis of lens epithelial cells.
6. Mechanisms and Medication Rules of Type 2 Diabetes Mellitus Based on Data Mining and Network Pharmacology
Ming BAI ; Bao-song LIU ; Meng-fan PENG ; Tian-yuan LIU ; Quan JIANG ; Ming-san MIAO
Chinese Journal of Experimental Traditional Medical Formulae 2019;25(9):182-188
Objective: To analyze the medication rules of traditional Chinese medicine (TCM) in treating type 2 diabetes mellitus (T2DM),and explore the mechanism of action.Method: The articlesabout TCM treatment for T2DM in CNKI and Wanfang Med Online were retrieved.The data was entered into Excel 2013 to set up a database,and the factor analysis,association rules and cluster analysis were used to analyze core medication combinations by SPSS Statistics 19.0 and SPSS Modeler 14.1.kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed using BATMAN-TCM to reveal the mechanism of action.Result: A total of 92 prescriptions were included,the high frequency medicines were Astragali Radix.The major properties of TCM were cold and plain.The major flavors of TCM were sweet and bitter.The major meridian tropism of TCM was livermeridian.These high frequency medicines were deficiency-nourishing herbs,and blood circulation-invigorating and stasis-eliminating herbs.Totally 6 core medication combinations were mined through association rules.Seven common factors were extracted from factor analysis.Clustering analysis was used to get 6 kinds of herbs.KEGG pathway analysis revealed 24 biological pathways and 6 diseases relating to T2DM.Conclusion: The most commonly used Astragali Radix for treating T2DM.In clinical,it can be combined with Poria,Salviae Miltiorrhizae Radix et Rhizoma and Rehmanniae Radix for treating T2DM.At the same time,it can be combined with such herbs as deficiency-nourishing herbs,and blood circulation-invigorating and stasis-eliminating herbs.The mechanism of action of high-frequency herbs may be related to the targets of exophosphatase/phosphodiesterase (PDEs),adenosine A1 receptor gene 1(ADORA1),adenosine A2A receptor gene (ADORA2A),neuropetide second receptor (NPY2R),and endothelial carbon monoxide synthase (NOS3) by interfering with PI3K/Akt,starch and sucrose metabolism signaling pathways.
7.Comparative study on establishing an immune tolerance model for allergic conjunctivitis in mice with different methods
Meng-Tian BAI ; Yun LI ; Zhu-Lin HU
International Eye Science 2022;22(10):1615-1620
AIM:To establish an immune tolerance model for allergic conjunctivitis in newborn mice with different methods and observe the impact of environmental factors on allergic conjunctivitis in early life.METHOD: A total of 50 Balb/c newborn mice were randomly divided into blank control group, ovalbumin(OVA)+subcutaneous injection group, OVA+nebulized inhalation group, OVA+gastric group, ragweed pollen(RW)+subcutaneous injection group, RW+nebulized inhalation group, RW+gastric group, house dust mite(HDM)+subcutaneous injection group, HDM+nebulized inhalation group, HDM+intragastric group(n=5 animals/group). Except for the blank control group, mice in each group were individually exposed to the corresponding antigens to induce immune tolerance early in life and stimulated with the corresponding antigens in adulthood. The ocular surface was visualized by anterior segment photography. The relative expression level of conjunctival RANTES and IL-17 mRNA was measured by RT-qPCR and serum IL-17 concentration was measured by ELISA.RESULTS: Compared with the blank control group, the relative expression level of conjunctiva IL-17 mRNA in RW+gastric group was the highest, and it was the lowest in RW+subcutaneous group(all P<0.05). The relative expression level of conjunctiva RANTES mRNA was the highest in RW+gastric group(P<0.001). Compared with the blank control group, the serum concentration of IL-17 was increased in all treatment groups except OVA+nebulizer group and RW+subcutaneous group(P<0.05).CONCLUSION: The immune tolerance of allergic conjunctivitis induced by subcutaneous injection of antigen was the most suitable method in the early life of mice.
8.Comparison of DK crush with classical crush technique with drug-eluting stents for the treatment of coronary bifurcation lesions from DKCRUSH-1 study.
Shao-liang CHEN ; Jun-jie ZHANG ; Fei YE ; Yun-dai CHEN ; Shu-zheng LÜ ; Huaycheem TAN ; Tejas PATEL ; Kawajiri KENJI ; Israel TAMARI ; Shou-jie SHAN ; Zhong-sheng ZHU ; Song LIN ; Nai-liang TIAN ; Xiao-bo LI ; Zhi-zhong LIU ; Michael LEE ; Meng WEI ; Ya-wei XU ; Zheng-bai YUAN ; Jun QIAN ; Xue-wen SUN ; Song YANG ; Jin-guo CHEN ; Ben HE ; Suji SUMIT
Chinese Journal of Cardiology 2008;36(2):100-107
OBJECTIVETo determine independent factors correlated with clinical effects of DK crush and classical crush technique with drug-eluting stents on bifurcation lesions.
METHODS311 patients with bifurcation lesions were randomized to classical (C, n = 156) or double kissing (DK) crush (n = 155) stent implantation group. The primary endpoints included major adverse cardiac events (MACE).
RESULTSFinal kissing balloon inflation (FKBI) success rate was 76% in C and 100% in DK groups (P < 0.001). DK crush procedure was characterized by lower unsatisfactory FKBI rate (27.6% vs.6.3%, P < 0.01). Clinical follow-up was available in 100% and angiographic follow-up in 82% patients. The overall restenosis rate was 32.3% in C and 20.3% in DK groups (P = 0.01), respectively. Cumulative 8-month MACE was 35.9% in without-FKBI and 19.7% in with-FKBI sub-groups, and 11.4% in DK group (P = 0.02). The incidence of stent thrombosis was 3.2% in C group (5.1% without vs. 1.7% with FKBI) and 1.3% in DK group (P > 0.05). The predictive factors of MACE included minimal side branch stent lumen diameter and lack of DK crush technique.
CONCLUSIONDK crush technique is an alternative of double stenting techniques in terms of improvement of restenosis and clinical outcomes.
Aged ; Angioplasty, Balloon, Coronary ; methods ; Coronary Artery Disease ; therapy ; Coronary Stenosis ; therapy ; Drug-Eluting Stents ; Female ; Humans ; Male ; Middle Aged ; Prospective Studies ; Stents
9.Human Gut Microbiota and Gastrointestinal Cancer.
Changting MENG ; Chunmei BAI ; Thomas D BROWN ; Leroy E HOOD ; Qiang TIAN
Genomics, Proteomics & Bioinformatics 2018;16(1):33-49
Human gut microbiota play an essential role in both healthy and diseased states of humans. In the past decade, the interactions between microorganisms and tumors have attracted much attention in the efforts to understand various features of the complex microbial communities, as well as the possible mechanisms through which the microbiota are involved in cancer prevention, carcinogenesis, and anti-cancer therapy. A large number of studies have indicated that microbial dysbiosis contributes to cancer susceptibility via multiple pathways. Further studies have suggested that the microbiota and their associated metabolites are not only closely related to carcinogenesis by inducing inflammation and immune dysregulation, which lead to genetic instability, but also interfere with the pharmacodynamics of anticancer agents. In this article, we mainly reviewed the influence of gut microbiota on cancers in the gastrointestinal (GI) tract (including esophageal, gastric, colorectal, liver, and pancreatic cancers) and the regulation of microbiota by diet, prebiotics, probiotics, synbiotics, antibiotics, or the Traditional Chinese Medicine. We also proposed some new strategies in the prevention and treatment of GI cancers that could be explored in the future. We hope that this review could provide a comprehensive overview of the studies on the interactions between the gut microbiota and GI cancers, which are likely to yield translational opportunities to reduce cancer morbidity and mortality by improving prevention, diagnosis, and treatment.
Anti-Bacterial Agents
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therapeutic use
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Gastrointestinal Microbiome
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drug effects
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Gastrointestinal Neoplasms
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microbiology
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prevention & control
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Gastrointestinal Tract
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microbiology
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Humans
10.Study on HPLC fingerprint of Sabia parviflora.
Wen-Fen XU ; Guo-Ji PAN ; Qing-Wen SUN ; Cai-Hong BAI ; Meng-Ge LIU ; Qu LIU ; Tian-Jin YANG
China Journal of Chinese Materia Medica 2019;44(21):4670-4676
This research was performed to establish the HPLC fingerprint of Sabia parviflora. HPLC method was carried out on a Thermo Accucore-C18(4. 6 mm×150 mm,2. 6 μm) column by 30% tetrahydrofuran in methyl alcohol-acetonitrile-0. 1% phosphate solution as mobile phase at a flow rate of 1. 0 m L·min-1,the column temperature was 30 ℃ and the detection wavelength was 360 nm. The fingerprints were further evaluated by chemometrics methods including similarity analysis,hierarchical clustering analysis,and principal component analysis. In HPLC fingerprint,15 common peaks were selected as the common peaks,and 6 contents of them were identified. The similarity degrees of 38 batches of the samples was more than 0. 710,and the samples were divided into 6 clusters by their quality difference. The method was precision,repeatable,stable,simple and reliable,which could be used for quality control and evaluation of S. parviflora.
Chromatography, High Pressure Liquid
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Cluster Analysis
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Drugs, Chinese Herbal
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Principal Component Analysis
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Quality Control