1.The pathogenic composition and molecular typing of hospitalized children with hand, foot and mouth disease in Tianjin in 2016
Rui PAN ; Shuxiang LIN ; Jianbo SHU ; Yingtao MENG ; Yingxue ZOU ; Lin PENG
Chinese Journal of Laboratory Medicine 2017;40(5):367-371
Objective To explore pathogenic composition of hand,foot and mouth disease(HFMD),and the molecular typing of enterovirus in Tianjin Children′s Hospital in 2016.Methods Single center study.A total of 327 samples of HFMD cases which collected from Tianjin Children′s Hospital from March to November in 2016 were tested for nucleotide acid of enterovirus(EV),human enterovirus 71(EV71),Coxsackievirus A 16(CA16)by real-time reverse transcription polymerase chain reaction(RT-PCR),among which 104 sample of other EV positive were selected to amplify and sequence the whole VP1 region by using RT-PCR.Homology was analyzed and phylogenetic tree were constructed by comparison of the sequence with all subgenotype of EV by Chromas1.62 and MEGA6.06.EV positive rate in different age groups were compared by SPSS20.0.Results Of all the 327 HFMD cases tested,there were 272 EV positive cases,the constituent ratio of EV71,CA16 and other EV were 55.1%(150/272),6.6%(18/272)and 38.2%(104/272)respectively.The EV positive rates of different age groups(69.0%-90.9%)were different significantly(x2=15.897,P=0.044),the 3 years-old-group had the highest EV positive rate than that of the other age groups(90.9%,40/44).Of all 104 samples of other EV tested,34 were CA10(12.5%)and 14 were CA6(5.1%).Phylogenetic analysis of 11 EV71 VP1 and 20 CA10 VP1 showed that the EV71 and CA10 strains belonged to genotype C4a and genotype G,respectively.ConclusionsEV71 was still the predominant pathogen in spite of the constituent ratio of other EVs increased markedly in 2016 in Tianjin.It is important to enhance etiological monitoring for control and prevention of HFMD.
2.Effects of Cordyceps Sinensis and Tipterygium wilfordii Polyglycosidium on the podocytes in rats with diabetic nephropathy.
Li HAO ; Meng-Shu PAN ; Yun ZHENG
Chinese Journal of Integrated Traditional and Western Medicine 2012;32(2):261-265
OBJECTIVETo investigate the effects of Cordyceps Sinensis (CS) and Tripterygium wilfordii Polyglycosidium (TWP) on the podocytes in rats with diabetes nephropathy (DN).
METHODSRat models of DN were established and rats were randomly divided into the normal control group (Group A), the DN group (Group B), the CS group (Group C), the TWP group (Group D), and the CS combined TWP group (Group E). The changes of 24-h urinary protein count (24-h pro), blood glucose (Glu), blood urea nitrogen (BUN), serum creatinine (SCr), white blood cell (WBC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and kidney weight/body weight (KW/BW) were determined 12 weeks later. The renal pathological changes were evaluated by HE staining. The microstructural changes of podocytes were observed by transmission electron microscope. The expressions of nephrin and podocin were detected by immunofluorescence staining.
RESULTSCompared with Group A, the levels of SCr and BUN were higher (P < 0.05), Glu, KW/BW, and 24 h pro were obviously higher in Group B (P < 0.01). There were less glomerular disorder, chronic tubulointerstitial damage and glomerular podocyte lesion in Group B. The expressions of the massive cortical nephrin and podocin protein were obviously less in Group B. Compared with Group B, the KW/BW and 24 h pro in Group C, D, and E were lower (P < 0.01). The pathomorphological improvement was exhibited in the glomerulus, tubules, and podocytes. The protein expressions of nephrin and podocin were higher in the renal cortex. Compared with Group C, the KW/BW and 24-h pro decreased in Group D and E, the expressions of nephrin and podocin were enhanced, and the lesions of the glomerulus, tubules, and podocytes were alleviated, showing statistical difference (P < 0.01). The most significant changes happened in Group E.
CONCLUSIONSCS and TWP could alleviate the DN proteinuria, protect and repair podocytes of DN rats. Its mechanisms might be correlated with up-regulating the expressions of nephrin and podocin. The combined use of CS and TWP could increase the efficacy and attenuate adverse reactions of TWP.
Animals ; Cordyceps ; Diabetes Mellitus, Experimental ; Diabetic Nephropathies ; pathology ; Male ; Podocytes ; drug effects ; Rats ; Rats, Sprague-Dawley ; Tripterygium
3.Autoregressive analysis of flash evoked potentials in healthy preterm infants during sleep.
Xiao-Long CHEN ; Xiao-Li PAN ; Shu-Ying MENG
Acta Physiologica Sinica 2002;54(5):446-450
To interpret the flash evoked potential (FVEP) as dynamic high-order responses to natural and experimental stimulation in healthy preterm infants, waveform analysis of FVEP in 36 healthy preterm infants (postconceptional age 28~42 weeks) were performed using an autoregressive analysis. Based on the histogram of damping frequency of different component impulse response waveforms, the waveforms were divided into 4 groups: group I (0 ~ <2 Hz), group II (2 ~ <6.5 Hz), group III (6.5 ~ <12.0 Hz) and group IV (12~25 Hz). The total power, power of component impulse responses (group I~IV), and damping time (group II~IV) changed significantly with increasing postconceptional age (P<0.01 or P<0.05). Identification of an impulse response component with dominant frequency which undergoes a well-identified change with age is considered to be a useful tool for discriminating between normal and abnormal changes in the FVEP with age in healthy preterm infants.
Evoked Potentials, Visual
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physiology
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Female
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Humans
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Infant, Newborn
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Infant, Premature
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physiology
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Male
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Reaction Time
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Sleep
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physiology
5.Development of ZM-1 tissue microarrayer.
Pan-qing MENG ; Gui-ying ZHOU ; Gang HOU ; Jia-ping PENG ; Qi DONG ; Shu ZHENG
Chinese Journal of Medical Instrumentation 2005;29(4):263-266
ZM-1 tissue microarrayer designed by our group is manufactured in stainless steel and brass. It features an easier and faster preparation for tissue microarrays. By means of it, a group of biopsy needles are used to punch the donor tissue specimens respectively, and all the needles with the punched specimen cylinders are arranged into the array-board, where small holes have been digged to fit the needles. All the specimen cylinders arraying and the tissue microarray block's shaping are finished simultaneously. ZM-1 tissue microarrayer with a lower cost of manufacture, is capable of preparing the tissue microarrays conveniently, efficiently and quality-controllably.
Equipment Design
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Tissue Array Analysis
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instrumentation
6.Application of new tissue microarrayer-ZM-1 without recipient paraffin block.
Pan-Qing MENG ; Gang HOU ; Gui-Ying ZHOU ; Jia-Ping PENG ; Qi DONG ; Shu ZHENG
Journal of Zhejiang University. Science. B 2005;6(9):853-858
The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method's relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.
Biopsy, Needle
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instrumentation
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Equipment Design
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Female
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Humans
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Immunohistochemistry
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Male
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Neoplasms
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enzymology
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Paraffin
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Tissue Array Analysis
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instrumentation
7.Preparation of sinomenine microemulsion and its transdermal absorption.
Xian-Zhou ZHANG ; Hong-Da ZHU ; Shu-Fang MENG ; Xi-Gui PAN
China Journal of Chinese Materia Medica 2007;32(19):2007-2010
OBJECTIVETo screen the optimum formulation and prepare O/W sinomenine microemulsion and investigate its in vitro transdermal delivery ability.
METHODThe microemulsions were prepared with the formulation containing oleic acid-tween 80-dehydrated alcohol-water by the pseudo-ternary phase diagram. The permeation flux of sinomenine was determined in vitro by Franz diffusion cell fitted with rat skin. The sinomenine was determined by HPLC. The transdermal characteristics of sinomenine microemulsion were compared with that of sinomenine gels.
RESULTThe steady state flux of sinomenine microemulsion was significantly higher than that of sinomenine gels. The average permeation rate of sinomenine microemulsion was 116. 44 microg x cm(-2) x h(-1) in vitro.
CONCLUSIONThese results indicated that the studied microemulsion system with high permeation rate may be a potential vehicle for the transdermal delivery of sinomenine.
Administration, Cutaneous ; Animals ; Drug Compounding ; methods ; Drug Delivery Systems ; Emulsions ; Ethanol ; chemistry ; Male ; Morphinans ; administration & dosage ; isolation & purification ; pharmacokinetics ; Oleic Acid ; chemistry ; Particle Size ; Plants, Medicinal ; chemistry ; Polysorbates ; chemistry ; Rats ; Sinomenium ; chemistry ; Skin ; metabolism ; Skin Absorption ; Surface-Active Agents ; chemistry
8.Clinical analysis of 15 851 children at risk of inherited metabolic diseases.
Shu-Xiang LIN ; Jian-Bo SHU ; Chao WANG ; Rui PAN ; Ying-Tao MENG ; Chun-Hua ZHANG ; Bi-Li ZHANG ; Dan WANG ; Yu-Qin ZHANG
Chinese Journal of Contemporary Pediatrics 2017;19(12):1243-1247
OBJECTIVETo explore the value of urine gas chromatography-mass spectrometry (GC-MS) in the screening of children at risk of inherited metabolic diseases (IMD), and to identify the disease spectrum of IMD and the clinical characteristics of children with IMD.
METHODSThe clinical data of 15 851 children at risk of IMD who underwent urine GC-MS in the Tianjin Children's Hospital between February 2012 and December 2016 were retrospectively analyzed.
RESULTSIn the 15 851 children, 5 793 (36.55%) were detected to have metabolic disorders. A total of 117 (0.74%) children were confirmed to have IMD, including 77 cases of methylmalonic acidemia (65.8%). The clinical manifestations of confirmed cases in the neonatal period mainly included jaundice, metabolic acidosis, abnormal muscular tension, feeding difficulty, poor response, and lethargy or coma. The clinical manifestations of confirmed cases in the non-neonatal period mainly included delayed mental and motor development, metabolic acidosis, convulsion, recurrent vomiting, and anemia.
CONCLUSIONSGC-MS is an effective method for the screening for IMD in children at risk. Methylmalonic acidemia is the most common IMD. The clinical manifestations of IMD are different between the confirmed cases in the neonatal and non-neonatal periods.
Acidosis ; etiology ; Adolescent ; Amino Acid Metabolism, Inborn Errors ; complications ; diagnosis ; Child ; Child, Preschool ; Developmental Disabilities ; etiology ; Female ; Gas Chromatography-Mass Spectrometry ; Humans ; Infant ; Infant, Newborn ; Male ; Metabolism, Inborn Errors ; complications ; diagnosis ; Retrospective Studies ; Risk
9.Application of Ligasure vessel sealing instrument in laparoscopic hepatectomy for liver cancer.
Tian-qi LIU ; Jian-qiang LUO ; Xin-ping LI ; Jin-fei PAN ; Dong HUANG ; Yun-tian TANG ; Meng-yang LI ; Zhong-xiao LIANG ; Shu-sen ZHENG
Journal of Southern Medical University 2010;30(7):1705-1706
OBJECTIVETo investigate the indication and effect of the application of Ligasure vessel sealing instrument in laparoscopic hepatectomy for liver cancer.
METHODSEleven patients with liver cancer undergoing laparoscopic hepatectomy were analyzed for the tumor size and location, operation time, volume of intraoperative bleeding, postoperative hospital stay and short-term clinical outcomes.
RESULTSAll the operations were performed successfully in the 11 cases. All the tumors were less than 7 cm in diameter, locating at the segments II, III, V, VI and VII. The mean operation time was 91 min (80-126 min), and the intraoperative blood loss averaged 82 ml (20-200 ml). The average postoperative hospital stay of the patients was 8 days (7-9 days). No complications were observed in these cases.
CONCLUSIONLigasure vessel sealing instrument in laparoscopic hepatectomy is applicable in cases of perimeter liver cancer. This instrument can decrease the operation time, reduce the intraoperative blood loss and postoperative hospital stay with good safety and minimal invasiveness.
Adult ; Aged ; Female ; Hepatectomy ; instrumentation ; methods ; Humans ; Laparoscopy ; Liver Neoplasms ; surgery ; Male ; Middle Aged ; Treatment Outcome
10.Effect of air pollution on respiratory health in school-aged children in the main urban area of Chongqing, China.
Ming-Yue FAN ; Xu TANG ; Wei HUANG ; Hua DAI ; Xing-Can LIU ; Yin-Yin XIA ; Pan MENG ; Rui-Yuan ZHANG ; Yu-Ming GUO ; Shu-Qun CHENG
Chinese Journal of Contemporary Pediatrics 2017;19(4):436-440
OBJECTIVETo investigate the effect of air pollution on respiratory health in school-aged children in the main urban area of Chongqing, China.
METHODSThe main urban area of Chongqing was divided into polluted area and clean area according to the air pollution data shown on the Environmental Protection Agency Website of Chongqing between 2010 and 2015. A cluster sampling method was used to select 695 third- or fourth-grade children from 2 primary schools in the clean or polluted area as study subjects, with 313 children from the clean area and 382 children from the polluted area. Pulmonary function was examined for all children and a standard American epidemiological questionnaire (ATS-DLD-78-C) was used to investigate the prevalence of respiratory diseases and symptoms.
RESULTSCompared with the clean area, the polluted area had significantly higher concentrations of inhalable particles (PM), fine particulate matter (PM), and nitric oxide (NO) (P<0.05). The multivariate logistic regression analysis was performed after adjustment for confounding factors, and the results showed that compared with those in the clean area, the children in the polluted area had significantly higher risks of cough (OR=1.644), cough during cold (OR=1.596), expectoration during cold (OR=2.196), persistent expectoration (OR=1.802), and wheezing (OR=2.415). The boys and girls in the clean area had significantly higher forced vital capacity and forced expiratory volume in one second than those in the polluted area (P<0.05).
CONCLUSIONSAir pollution in the main urban area of Chongqing is associated with the increased prevalence of respiratory symptoms in school-aged children and has certain effect on children's pulmonary function.
Air Pollution ; adverse effects ; Child ; Female ; Forced Expiratory Volume ; Humans ; Logistic Models ; Male ; Respiratory Tract Diseases ; etiology ; Vital Capacity