OBJECTIVE:To discuss the application of Prescription Automatic Screening System (PASS) in the examination of clinical medical orders. METHODS: The level and type of caution and the modification rate of medical orders in our hospital between April 2005 and April 2006 were analysed statistically, and the conformity between PASS cautions of medical orders and clinical medications was evaluated. RESULTS: A total of 10 982 160 medical orders were monitored by PASS, of which, 11 146 were with black cautions and in which 2 861(25.67%) were modified, 51 504 were with red cautions and in which 10 357 (20.0%) were modified, 339 449 were with yellow ones and in which 66 022 (19.45%) were modified. In PASS monitor process, inconformity between feasible clinical medical orders and cautions of PASS was noted in a few cases. CONCLUSION: The examination of medical orders using PASS is convenient and rapid and the PASS can help improve clinical rational drug use level, however, its function of remains to be updated and optimized.

Objective To evaluate the prognosis and influencing factors of unprotected left main coronary artery lesion intervention with drug-eluting stents.Methods Forty-five patients with unprotected left main coronary artery disease were treated with percutaneous coronary intervention (PCI).According to whether the lesions involving the distal left main bifurcation,the patients were divided into two 2 groups:nonbifurcation lesion group (26 cases) with opening or body disease,bifurcation lesion group (19 cases) with involving the distal left main or anterior descending artery,circumflex openings.Fifteen patients were guided by intravascular ultrasound (IVUS) during PCI,and 13 patients were reviewed by coronary angiography at (9.5 ±6.3) months after PCI.Results All the 45 patients were successful.There were 42 patients successfully finished the follow-up study for the average of 8-48 (19.9 ± 10.2) months,non-bifurcation lesion group was 24 cases,bifurcation lesion group was 18 cases.The incidence of recurrent angina pectoris during the follow-up period in bifurcation lesion group was significantly higher than that in non-bifurcation lesion group [6/18 vs.12.5％ (3/24)],there was statistical difference (P ＜ 0.05),but there was no statistical difference in the incidence of major adverse cardiac events (MACE) between 2 groups [3/18 vs.12.5％ (3/24),P ＞ 0.05].Single-variable analysis results showed that MACE in IVUS guided during PCI was less than that in non-IVUS guided during PCI [1/15 vs.18.5％(5/27)],there was statistical difference (P ＜0.05).In 13 patients who reviewed by coronary angiography,4 patients were found in-stents restenosis,all in bifurcation lesion group,the rate of restenosis in double stents was 3/6,in simple stents was 1/4.Conclusions PCI with drug-eluting stents is safe and feasible for the treatment of selective left main coronary artery lesion,it could bring the better short and long-term outcomes.IVUS guided PCI could significantly reduce MACE.

Objective To realize job satisfaction of doctors from the secondary hospital and its influencing factors and put forward countermeasures for doctor job satisfaction.Methods 2066 doctors from the secondary hospital were investigated by self-made scale,and analyzed by analysis of variance,non-conditional logistic regression analysis and other methods.Results The average score of total job satisfaction of doctors was (3.44±0.95),in which about 57.6% of doctors expressed satisfaction with the current work.The scale of job itself satisfaction had the hisheet score,that was (3.91±0.79),while the scale of job retum satisfaction had the lowest score,that was (2.85±1.16).Leadership behavior played the most decisive role in doctors job satisfaction(sβ=0.7,P<0.01).Condusion The total job satisfaction of secondary hospital doctors is just above the average and doctors are most dissatisfied with the work concentrated in the areas of return and the practice environment.

Objective:To study B cells proliferation and activation induced by tumor derived-autophagosomes ( TDA). Methods:Splenocytes of mice were incubated with 10μg/ml TDAs in vitro ( with the 10μg/ml whole cell lysate as the control).At the 3rd day,the expression of MHCⅠ,Ⅱand co-stimulate molecules including CD86,CD40 were detected by flow cytometry,and B cells proliferation was detected at the 5th day.B cells were selected from spleen of mice using anti-CD43 dynabeads,and incubated with 10μg/ml TDA in vitro ( with the same concentrated whole tumor cell lysate as the control).At the 7th day,IgM in the supernatant were tested by ELISA.Results:When compared with whole cell lysate stimulated B cells,TDA efficently stimulated B cells division in vitro ( TDA group:28.6%, Whole cell lysate group:4.4%) , and significantly up-regulated the expression of MHC class Ⅰ,Ⅱ and co-stimulatory molecules (CD86 and CD40) on B cells,and enhanced the levels of total IgM secretion in vitro.Conclusion:Tumor-derived autophagosomes ( TDA) ,as a vacuole antigen vector, could stimulate B cells proliferation, activation, and increased IgM secretion in vitro.

ObjectiveTo investigate the clinical efficacy of focused ultrasound for treating non-neoplastic epithelial disorders of vulvar. Methods212 patients with non-neoplastic epithelial disorders of vulvar were treated with focused ultrasound. The patients were followed up after the treatment,and symptom was observed and the efficacy was evaluated. ResultsThe efficacy of non - neoplastic epithelial disorders of vulvar of squamous hyperplasia,lichen sclerosus and mixed dystrophy was 99.2％, 100. 0％, 88.9％ respectively, the difference was not significat (P ＞ 0.05 ). The efficacy in different courses of patients was 100.0％ ( ＜ 2 years), 100.0％ (2 ～10 years) ,91.3％( ＞ 10 years) respectively,the difference was not significat( P ＞ 0.05). ConclusionFocused ultrasound was obviously effective in the treatment of patients with non-neoplastic epithelial disorders of vulvar.

AIM:To evaluate the clinical effects of femto-LASIK using new Ziemer LDV Z6 femtosecond laser machine (Z6).
METHODS: Two - hundred cases ( 400 eyes ) was randomly separated into two groups: Group A included 200 eyes which corneal flaps were made by Z6, and Group B included rest of 200 eyes which corneal flaps were examined by a traditional Ziemer LDV CrystalLine femtosecond laser machine (CrystalLine). Visual acuity, slit lamp, refraction, Sim-K average, intraocular pressure (IOP), non-invasive tear break-up time (NIAvg-BUT), operation difficulty and complications were compared between two groups preoperatively and postoperatively.RESULTS:There was no significant differences between two groups in visual acuity, refraction, Sim-K average, IOP and NIAvg - BUT either preoperatively or 6mo postoperatively (P>0. 05). Although there were significant differences in operation difficulty and complications were found between two groups ( P< 0. 05 ), there was no serious complication in any group. Furthermore, a clear demarcation line could be observed in the cornea of Group A postoperatively.
CONCLUSION:More careful and strict requirements are needed when using the new Z6 femtosecond laser for corneal flaps.

Objective To investigate the protective effect of the ethanol extract of vacciniuin bracteatum thumb leaf on acute liver injury induced by CC14 in mice. Methods Sixty mice were allocated into 6 groups randomly, respectively as the normal control group,the model group,the positive control group,the ethanol extract low,middle and high dose group. Each group had 10 mice. Acute hepatic injury model of mice was made by using CC14. The level of ALT, AST in blood serum and SOD,MDA in hepatic tissue were observed. The hepatic tissue was HE-stained conventionally and observed. Results Acute liver injury in mice could be successfully induced by CC14 ,as demonstrated by increased serum ALT, AST levels and severe histological observations. Pretreatment with the ethanol extract of vaccinium bracteatum thumb leaf indicated its potent protective effects on acute liver injury, as demonstrated by a significant decrease in ALT, AST and MDA. Conclusion The ethanol extract of vaccinium bracteatum thumb leaf had an obviously protective effect on acute liver injury induced by CC14, which could be due to its antioxidative stress effect.

OBJECTIVE: To explore the way to develop online pharmacy in China. METHODS: The online pharmacy was compared between China and the USA in respect of authentication, qualification of enterprises, distribution of medicines, management scope, payment, and protection of consumers' interest; and the implication of the development of the USA online pharmacy for China was analyzed. RESULTS & CONCLUSIONS: In the development of online pharmacy, China should draw experiences from the USA to introduce multiform management model, carry out multi- level online drug quality control, and to establish measures such as the compensation system for the drug induced sufferings.

Objective To evaluate the outcomes of transanal coloanal pull-through with resection of the internal anal sphincter in neonates and infants with Hirschsprung's disease.Methods Transanal coloanal pull-through with resection of the internal anal sphincter was performed on 101 neonates or infants with Hirschsprung's disease in our hospital.An incision slightly longer than one-half of the rectal circumference was made at the dentate line.Then,the muscular layer of the anterior rectal wall was dissected and separated upwards from the submucosal layer;meanwhile that of the posterior wall was also separated upwards along the rectal longitudinal muscles.After the muscular layer was separated to the level of the peritoneal reflection,a radial operation was performed by resecting the internal anal sphincter and muscle sheath of the posterior rectal wall.Results The operation was accomplished successfully in all the patients with less than 10 ml of intraoperative blood loss.The operation time ranged from 45 to 190 min(mean,90 min).No complications,including urine retention,incision infection,and anastomotic stenosis,occurred.Two patients(2/86,2.3%) developed enterocolitis after the operation.The incidence of fecal soiling was 27%(22/82) at 2 months after the operation,and then decreased to 4%(3/82) at 6 months.Anorectal manometry was performed on 78 patients at 1,2,3,and 6 months postoperatively.The results demonstrated that the anal resting pressure after the operation was significantly lower than that before the operation(P

Objective To evaluate the antibacterial activity in vitro of Galla Chinensis ethanol extractant(GCEE)against S.epidermidis,and the change of cell morphology of S.epidermidis.Methods Deterimination of antibacterial activity of GCEE against 124 strains S.epidermidis was performed by using agar dilution method,and the changes of cell merphalogy of S.epidermidis was examined by microscope.Results The MIC90 of GCEE against 43 strains of MRSE(methicillin-resistant staphylococcus epidermidis)and 81 strains of MSSE(methicillin-sensitive staphylococcus epidermidis)were 0.288 and 0.144 mg/mL respectively.Under microscope,the cells of S.epidermidis were expansion by degrees,with the edge of cell of S.epidermidis irregular.The substances in cells of S.epidermidis were disappear final.Conclusion Galla Chinensis ethanol extractant had strong antibacterial activity against 124 strains of S.epidermidis,and the cell morphology of S.epidermidis changed.