Objective:To investigate effectiveness of exposure and ritual prevention of patients with obsession-compul-sive disorder(OCD),so as to provide references for professionals in clinical psychology.Methods:One female patient with severe OCD received 3 weeks intensive exposure and ritual prevention treatment.In order to investigate the effectiveness,she completed Symptom Check List 90 and Yale Brown Obsessive Compulsive Scale(SCL-90 and YBOCS) at pre-treat-ment,mid-treatment and post-treatment.Results:After treatment,the patient's scores of questionnaires reduced,such as score on YBOCS reduced from 38 to 9.She reported that her washing time reduced obviously and her own measure of dis-tress index reduced.Conclusion:Exposure and ritual prevention could treat the patient with OCD effectively.Her distress reduced and life function was improved.

Objective: To investigate the current situation of consumption and development tendency of antitumor Chinese herbal injections in Wuhan area. Methods:The analytical method of the defined daily dose ( DDD) recommended by WHO was used to ana-lyze the application of antitumor Chinese herbal injections in 32 hospitals of Wuhan area during the period of 2010-2012. Results:The consumption sum and the DDDs of antitumor Chinese herbal injections were increased year after year, while the daily drug cost ( DDC) was stable in general. The sort of consumption was generally in accordance with the sequence of DDDs. Conclusion:The status quo of antitumor Chinese herbal injections during the period of 2010-2012 is in line with the characteristics of clinical application, indicating the use is rational.

Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional ; Neoplasms ; complications ; therapy

[Objective]To develop macroporous calcium phosphate cements(CPCs) by incorporating the gelatin microspheres(GMs) and to evaluate the performance of the composite as a bone substitute in vitro and in vivo. [Methods]GMs were prepared by improved emulsified cold-condensation method and macroporous CPCs were developed using GMs with three different weight ratios (0%,2.5% and 5%). The porosity and the compression strength of the three composites were measured to determine the proper weight ratio of GMs. The morphologic changes of osteoblasts were examined with scanning electron microscope at two days after the cells were seeded on the CPCs and GMs/CPCs. The cells were also cultured with different leaching liquor of three kinds of materials (0%,2.5% GMs/CPCs and polystyrene).Cell viability analysis was performed by MTT assay,and alkaline phosphatase was measured with alkaline phosphatase kit. At six months after implanted in the goat vertebre ,the samples with CPCs or GMs/CPCs were obtained and evaluated by X-ray image and histological anatomy.[Results]Porosity of the GMs/CPCs increased with the increase of GMs,but compression strength decreased. The proliferation and differentiation of the osteoblasts were enhanced with the 2.5% GMs/CPCs compared to the CPCs. In vivo study showed that the degradation of GMs/CPCs was improved.[Conclusion]Macroporous calcium phosphate cements can be developed by incorporating gelatin microspheres,and biocompatibility and degradability of the composite can be enhanced. The composite can be used as a bone substitute under non-loaded circumstances.

Objective To gain more pathophysiolgic knowledge about acute obstructive hydrocephalus and to explore its rapid and effective treatment by establishing canine acute obstructive hydrocephalus model.Methods Acute obstructive hydrocephalus model was established by injecting cyan-acrylic gel glue into the fourth ventricle via posterior fosse craniotomy in 9 male adult mongrel dogs.At the same time,lateral ventricle catheterization were performed and were fixed on the scalp to connect reservoir bag so that the changes of intracranial pressure (ICP) could be measured dynamically,and the changes of neurological function were observed.Results Acute obstructive hydrocephalus model was successfully established in 6 of the total 9dogs.ICP was (48.2 ± 6.1 ) cm H2 O at 48 hours after the injection and was (56.4 ± 5.7 ) cm H2 O at 72 hours after the injection,it increased 392％ and 459 ％ respectively.And the ICP after injection was significantly different(P ＜ 0.01 )compared with that before injection (12.3 ± 3.1 )cm H2O.Conclusion The establishment of acute obstructive hydrocephalus model has high success rate,and is easy to reduplicate; ICP could be measured dynamically and also could be reduced by releasing CSF;Thus,ventricular drainage is the most rapid and effective treatment for acute obstructive hydrocephalus.

Objective To localize Der f 2 in the body of Dermatophagoides farinae.Methods Live mites were embedded.Serial mite paraffin sections were made and checked under light microscope.The sections were incubated with anti-recombinant Der f 2 monoclonal antibody as the first antibody.They were then reacted with the fluorescent isothiocyanate conjugated goat anti-mouse IgG as the secondary antibody.The sections were examined with fluorescent light microscopy.Results Der f 2 reacting with immunofluorescent antibodies was found localized in the tissue and contents of the mid-gut of the mite.Conclusion The major allergen Der f 2 distributes in the gut and fecal pellets of Dermatophagoides farinae.

Objective To investigate the radiosensitization effect of the simultaneous silence of bcl-2 and XIAP on human head and neck cancer cell line in vitro.Methods Four groups of UMSCC12 cells were transfected with siRNA-bcl-2,siRNA-XIAP,siRNA-bcl-2 plus siRNA XIAP,and siRNA control,respectively.The silence efficiency was tested by Western blot assay. Apoptosis was evaluated with the activities of caspase-3 and caspase-9,and radiosensitization effect was evaluated with clonogenic assay.Results Bcl-2 and XIAP protein expressions were effectively eliminated in the cells simultaneously transfected with siRNA-bcl-2 and siRNA-XIAP.Transfection of cells with bcl-2 siRNA increased radiationinduced apoptosis ( t =5.32,6.27 ; P ＜ 0.05 ),but transfection with XIAP siRNA did not impact cell apoptosis.Since the simultaneous transfection of the above two siRNAs,the activities of caspase-3 and caspase-9 were increased by 1.36 and 1.34 times ( t =11.47,6.22 ; P ＜ 0.05 ) in nonirradiated cells and increased by 1.72 and 1.98 times ( t =12.02,20.14; P ＜ 0.05 ) in irradiated cells,respectively.The colonic assay showed that the SERs were 1.06,1.15 and 1.41 for the cells transfected with XIAP siRNA,bcl-2 siRNA and both siRNAs,respectively.Conclusions Compared to single silence of XIAP or bcl-2,simultaneous silence of XIAP and bcl-2 offers a potential approach to improving the radiosensitivity of the head and neck cancer cells,and apoptosis might contribute to the enhancement of radiosensitization.

Objective To investigate the therapeutic effectiveness of video-assisted thoracoscopic surgery(VATS)and thoracotomy for spontaneous pneumothorax in high altitude.Methods 256 concecutive patients diagnosed as having spontaneous pneumothorax in high altitude between July 1997 and August 2007 were analyzed retrospectively.VATS was performed in 158 cases and thoracotomy was performed in 98 cases.The operating time,blood loss during operation,chest drainage time,postoperative hospital stay,the costs of operation,the total costs of hospital stay and postoperative complication were compared between the two groups.Results The operating time in VATS group and thoracotomy group was(55.6?5.8)min and(66.3?5.1)min respectively(t=11.678,P=0.000),the blood loss during operation(40.6?5.8)ml and(120.3?6.5)ml respectively(t=11.678,P=0.000),the chest drainage time(2.6?0.7)d and(3.2?0.8)d,respectively(t =3.024,P=0.000),the postoperative hospital stay(4.8?0.9)d and(5.2?0.6)d respectively(t =4.168,P=0.000),the the costs of operation(9876?687)Renminbi Yuan and(4948?562)Renminbi Yuan respectively(t =44.532,P=0.000),the the total costs of hospital stay(17 028?956)Renminbi Yuan and(13 892?835)Renminbi Yuan respectively(t=21.743,P=0.000),the postoperative complication 12 cases and 38 cases respectively(?2 =21.351,P=0.000).The number of recurrence after operation was not statistical significant difference(?2=0.734,P=0.512).Conclusion VATS is accepted easily by patients and the total curative effect is dominant comparing to standard thoracotomy.The VATS method is the first selection for spontaneous pneumothorax in high altitude.But the defect of VATS is higher in the costs of operation and hospital stay.Decreasing applying the consumptive material of one time can reduce the costs.

[Objective]To analyze the biodegradation properties and active osteogenesis features of implanted composites of gelatin microspheres(GMs) impregnated with calcium phosphate cement(CPC) and recombinant human bone morphogenetic protein-2(rhBMP-2) in bone defect.[Method]rhBMP-2 loaded gelatin microspheres was incorporated in rhBMP-2/CPC during setting.Thirty rabbits were divided into groups A,B and C(10 each).The rhBMP-2/GMs /CPC,GMs/cpc and rhBMP-2/cpc was implanted into the rabbits of group A,B and C,respectively.The biodegradation properties and active osteogenesis features of implanted composites bone substitute was evaluated by radiography,histology,bone mineral density(BMD)after the composites were implanted into the bone defects at rabbit radius for 6,12 weeks respectively.[Result]Significant implant resorption and bone ingrowth were observed in all rhBMP-2/GM/CPC treated defects while the defects treated with rhBMP-2/CPC implants showed slight resorption of CPC and the defects treated with GM/CPC implants showed slight formation of new bone.[Conclusion]The result of this study suggested that the rhBMP-2/GMs/CPC composite could be used as material for bone graft substitute.

OBJECTIVE:To prepare compound Weifu emulsion and to observe its therapeutic effect on infantile eczema METHODS:The emulsion was prepared with a formulation consisting of Scutellaria,Chinese Angelica,Chuanxiong and dimethyl silicone oil etc and the quality control method was established RESULTS & CONCLUSION:This preparation is stable in quality and feasible in quality control;The effective rate in treating infantile eczema has reached 90% and no obvious adverse reactions were found This emulsion is worthy of recommendation for clinical use