Objective To observe the effect of bloodletting cupping on the serum insulin level in patients with simple obesity. Methods Fifty simple obesity patients were randomized into a treatment group and a control group, 25 in each group. The treatment group was intervened by bloodletting cupping, while the control group was by oral administration of Sibutramine Hydrochloride. The efficacy for losing weight and the serum insulin level were evaluated and compared before intervention and after 2 treatment courses. Results The total effective rate was 88.0%in the treatment group versus 80.0%in the control group, and the difference was statistically insignificant (P>0.05). The serum insulin levels were changed significantly in both groups after intervention (P＜0.05). After intervention, there was a significant difference in comparing the serum insulin level between the two groups (P＜0.05). Conclusion Bloodletting cupping can significantly improve the serum insulin level in patients with simple obesity.

Objective To investigate the pathogenic significance of antigenic epitopes and their relevant antibodies in pemphigus vulgaris (PV) by neonatal mouse model. Methods The extracellular domain 1-2 (EC1-2) fusion protein was expressed and purified by glutathione affinity chromatography on the basis of construction of recombinant EC1-2 vector, and then the New Zealand white rabbits were immunized to obtain the specific antisera. The IgG fraction was transferred into the neonatal mice passively after it was purified from the antisera. After 15-18 hours of injection, the abdomen skin and the sera of the mice were examined by light microscopy, electron microscopy, direct immunofluorescence and indirect immunofluorescence. Results In the evaluation of the study group of mice, the intraepithelial vesicle formation was observed. Electron microscopy showed that intercellular spaces were widened, desmosome split and disappeared. In immunofluorescence, the fluorescence-labeled IgG deposied between the acantholytic cells. In the control group of mice there were no pathogenic changes observed, except very weak fluorescence between intercellular spaces. Conclusion The PV mouse model established shows that the EC1-2 epitope in PVA antigen and its relevant antibodies were pathogenic, and can be used as a tool in studying the pathogenesis of PV.

Adolescent ; Adult ; Allyl Compounds ; poisoning ; Chronic Disease ; Female ; Humans ; Occupational Diseases ; diagnosis ; therapy ; Poisoning ; diagnosis ; therapy

Acute Disease ; Chemical and Drug Induced Liver Injury ; etiology ; Humans ; Male ; Middle Aged ; Toluidines ; poisoning ; Young Adult

Female ; Humans ; Mercury Poisoning ; therapy ; Young Adult

Burns, Chemical ; Eye Burns ; Humans ; Hydrogen Sulfide ; Male ; Occupational Injuries

Objective: To investigate the difference in influencing factors for diabetes between urban and rural residents in Hefei City, so as to provide the basis for control and research of diabetes. Methods: The residents aged 18 years and older were selected using the stratified multistage random sampling method from 5 districts (counties) in Hefei City from August to December 2021. Demographic information, smoking, self-rated health status and sleep duration were collected through questionnaire surveys. Height, body weight and fasting blood glucose were measured. The crude prevalence of diabetes was calculated and standardized by age using China Statistical Yearbook 2022. Factors affecting diabetes were identified using a multivariable logistic regression model. Results: Totally 10 443 residents were investigated, including 6 386 urban residents (61.15%) and 4 057 rural residents (38.85%). There were 4 690 males (44.91%) and 5 753 females (55.09%). Diabetes were detected in 1 492 cases, with a standardized prevalence of 9.57%. The standardized prevalence of diabetes among urban and rural residents were 9.21% and 12.58%, respectively, and the difference was statistically significant (P<0.05). Multivariable logistic regression analysis showed that region, age, educational level, occupation, body mass index and self-rated health status were influencing factors for diabetes. Further analysis stratified by urban and rural area showed that, in addition to the above factors, gender and smoking were influencing factors for diabetes among urban residents, while sleep duration was the influencing factor for diabetes among rural residents (all P<0.05). Conclusions There are urban-rural differences in the prevalence of diabetes among residents in Hefei City, and the prevalence of diabetes is associated with age, educational level, occupation, body mass index and self-rated health status.

OBJECTIVE:To investigate clinical efficacy and safety of Xingnaojing injection in the treatment of acute cerebral infarction,and its effects on neurological function,activities of living,inflammatory factors and coma score.METHODS:Ninety patients with cerebral infarction admitted in Chongqing People's Hospital from Jun.2015 to Jan.2016 were divided into control group and observation group according to random number table,with 45 cases in each group.Control group was given conventional treatment as Clopidogrel bisulfate tablets 75 mg,po,qd+ Nimodipine tablets 20 mg,po,tid.Observation group was additionally given Xingnaojing injection 20 mL added into 0.9％ Sodium chloride solution 250 mL,ivgtt,qd,on the basis of control group.Both groups received treatment for consecutive 14 d.Clinical efficacies of 2 groups were observed.NIHSS scores,Barthel indexes,Glasow coma scale and the levels of TNF-α and IL-6 were also observed before and after treatment.The occurrence of ADR was recorded.RESULTS:The total response rate of observation group (91.11％) was significantly higher than that of control group (73.33 ％),with statistical significance (P＜0.05).Before treatment,there was no statistical significance in NIHSS scores,Barthel indexes,Glasow coma scale or the levels of TNF-α and IL-6 between 2 groups (P＞0.05).After treatment,NIHSS scores,the levels of TNF-α and IL-6 in 2 groups were decreased significantly,while Barthel indexes were increased significantly;the improvement of above indexes in observation group were significantly better than control group,with statistical significance (P＜0.05).Fourteen days after treatment,Glasow coma scale of control group was significantly higher than before treatment;1,5,14 d after treatment,Glasow coma scale of observation group was significantly higher than before treatment,and significantly higher than that of control group,with statistical significance (P＜0.05).Total incidence of ADR in observation group (6.67％) was significantly lower than control group (13.33％),with statistical significance (P＜0.05).CONCLUSIONS:Xingnaojing injection shows significant therapeutic efficacy for acute cerebral infarction,can effectively improve the neurological function,activities of living,the regain of consciousness,and can reduce the level of inflammatory factor with good safety.

Objective To study the effect of single chain variable fragment (ScFv) antibody to EC3-4 fragment of desmoglein (Dsg) 3 in a mouse model of pemphigus vulgaris.Methods The ScFv an- tibody to EC3-4 fragment of Dsg-3 was injected subcutaneously into neonatal BALB/c mice at different time points;the mice were then evaluated clinically,histopathologically and by direct immunoflorescence exami- nation for the development of lesions.Results When injected alone,the ScFv antibody did not induce the appearance of key clinical features of pemphigus vulgaris.The antibody also did not prevent the develop- ment of pemphigus vulgaris features induced by sera of patients with pemphigus vulgaris,regardless of the time point of injection of ScFv antibody.Conclusion The ScFv antibody to EC3-4 fragment of Dsg-3 lacks pathogenicity in neonatal BALB/c mice,and also could not inhibit the development of lesions induced by sera from patients with pemphigus vulgaris.

Thiopurines, one kind of immunosuppressants, represent an effective and widely prescribed therapy in clinic. Howev-er, the narrow therapeutic window and pharmacokinetic individual differences are always the problems in the clinical application of these drugs. Many factors can affect the metabolism and pharmacological effects of thiopurines, and the genetic polymorphisms of relat-ed metabolic enzymes involved in the metabolic process are considered to be the main factors. Recently, there is growing attention to the influence of the pharmacogenetics of related metabolic enzymes on the pharmacokinetics and pharmacodynamics of thiopurines. Therefore, based on the literature data, this review summarizes the correlation between the genetic polymorphisms of related metabolic enzymes ( TPMT, ITPA, GST, HPRT, XO, IMPDH and GMPS) and efficacy and side effects of thiopurines in order to provide guid-ance for the individualized thiopurine treatment in the clinical practice.