Objective To investigate the relationship between plasma anti-β2-glycoprotein I (anti-β2-GP I )and cardiovascular disease (CVD) in systemic lupus erythematosus (SLE).Methods Eighty-onepatients with SLE [the mean age was (45±18) years old,among whom 73 were female and 8 were male] and20 controls [the mean age was (43±17) years old,among whom 14 were female,and 6 were male] wereenrolled.Plasma anti-β2-GP I was measured by ELLSA.The relationship between plasma anti-β2-GP I level and CVD in SLE patients was investigated with Logistic regression model.T-test,x2 test,Spearman's correlations and Logistic regression analysis were used for statistical analysis.Results Mean plasma anti-β2-GP I increased significantly in SLE group compared to control group [ (29± 19) vs (14±8) U/ml,t=2.035,P＜0.05].The plasma levels of anti-β2-GP I were higher in SLE patients with CVD than those without [(41±25)vs (18±12) U/ml,t=2.038,P＜0.05].Plasma anti-β2-GP I level was positively correlated with triglyceride (r=0.337,P＜0.05) and renal lesions (r=0.489,P＜0.01 ).Plasma anti-B2-GP [ level was negatively correlated with high density lipoproteins (r=-0.385,P＜0.05 ) and complement (r=-0.497,P＜0.05 ) level.Logistic regression analysis showed that plasma anti-β2-GP I (β=0.675,95％CI0.5070.816,P＜0.05) was an independent risk factor for CVD in SLE patients.Conclusion The level of plasma anti-β2-GP I in SLE patients with CVD is high,and it may play a role in the pathogenesis and progression of CVD in SLE patients.

Conventional CT plays an important role in the diagnosis, staging, radiotherapy target delineation, and prognosis of NSCLC, but it still has many limitations.PET/CT has been widely used in the diagnosis and treamtent of lung cancer.In particular, PET/CT metabolic parameters have great guiding significance in the prognosis of NSCLC patients undergoing radiotherapy, and may be superior to many clinical indices.This review summarizes the advances in the application of PET/CT in the prognostic evaluation of NSCLC patients undergoing radiotherapy.

Objective To explore the morbidity and clinical characteristics of knee osteoarthritis (OA) complicated with metabolic syndrome (MS).Methods Four hundred and ten patients with knee OA were enrolled into this survey.The subjects were divided into two groups:those with knee OA only:those with both OA and MS.Clinical data in the two groups were evaluated.Results The prevalence of MS was 40.2％ in all knee OA patients,there was significant difference between the two groups according to the ratio of male to female (x2=5.853,P=0.001),but no difference between the two groups according to age distribution respectively (P＞0.05).For metabolic parameters,waist circumference,triglyceride (TG),low density lipoprotein cholesterol (LDL-C),systolic blood pressure and glycosylated hemoglobin (HbA1c) were significantly increased in the group with both of the two diseases (OA and MS),comparing with those with knee OA only (t=3.123,4.679,2.818,3.697,2.632,2.907,P＜0.05).However,there was no difference in both group in serum total cholesterol (TC),high density lipoprotein cholesterol (HDL-C) and fasting plasma glucose level (P＞0.05).Incidence of coronary heart disease,diabetes mellitus and hypertension was higher in the group with both OA and MS than that in knee OA only group (x2=6.676 and 104.12,P＜0.05).The incidence of diabetes mellitus was not different between the two groups (P＞0.05).Conclusion The incidence of metabolic syndrome in knee OA patients is relatively high.Effective treatment should be invited to treat knee OA with MS.

Adult ; Aged ; Chlorine ; poisoning ; Female ; Follow-Up Studies ; Humans ; Lung ; physiopathology ; Male ; Middle Aged ; Young Adult

Cranial Sinuses ; surgery ; Diverticulum ; complications ; surgery ; Humans ; Male ; Middle Aged ; Tinnitus ; etiology ; surgery

Objective To study the double restriction fluorescent labeling (DRFL) method for fluorescent labeling of trace DNA samples and its effect in enhancing the pathogen detection sensitivity of microarray assays. Method SARS-CoV RNA samples were reversely transcribed and then further amplified with the restriction display (RD)-PCR and fluorescently labeled by conventional restriction labeling directly with Cy-universal primer and the novel double labeling with Cy-universal primer and CydNTP. The labeled samples were applied to the microarray with the viral probes, processed and analyzed. Results Compared with the conventional method, DRFL labeling resulted in 3. 5835 times higher fluorescent intensity of all the SARS probes on average, even though increased fluorescent intensities for different probes varied considerably. Conclusion Signal to noise ratio can be enhanced by the DRFL method which improves the sensitivity of microarray technology in trace pathogen detections.

Objective To evaluate the clinical effect of levocarnitine in the treatment of continuous blood purification and its effect on matrix metalloproteinase-2(MMP-2),interleukin-18(IL-18)and heart function.Methods 118 patients with continuous blood purification were randomly divided into the control group(n=59)and treatment group group(n=59).Two groups were treated with hemodialysis treatment.Control group was given folic acid,vitamin B and alpha keto acid,treatment group intravenous given levocarnitine injection 20mg/kg+0.9％NaCl 20mL on the basis of control group.A cycle of treatment was 28 days,and treated for 3 cycles.Serum levels of MMP-2 and IL-18,heart function,clinical effect and adverse drug reaction were compared between the two groups.Results After treatment,the total effective rate in control group was 84.74％,lower than 94.92％in treatment group(P<0.05).After treatment,serum MMP-2,IL-18,left atrial diameter(LAD),left ventricular end diastolic diameter(LVDd),left ventricular posterior wall thickness(LVPWT),ventricular septal thickness(IVST),left ventricular volume index(LVMI)in two groups were lower than pre-treatment(P<0.05),and the indexes in treatment group were more lower than control group(P<0.05).The E/A and left ventricular ejection fraction(LVEF)in two groups were all increased after treatment,and those in treatment group were higher than control group,the differences were significant(P<0.05).There was no significant in the incidence rate of adverse drug reactions between two groups.Conclusion Levocarnitine in the treatment of continuous blood purification was effective,and significantly reduce the serum MMP-2,IL-18 level and improve the heart function.

AIM To examine the antitumor activity of clostridium difficile toxin A. METHODS Highly purified toxin A from clostridium difficile was obtained by bovine thyroglobulin affinity chromatography followed by ion exchange chromatography steps on Q sepharose. The antitumor activity of toxin A of clostridium difficile on TPC 1 cell line was studied with Vero cell line as the normal cell line. The estimating ways used in this study were trypan blue exclusion test, MTT calorimetric assay, membrane damage test using 3H Uridine and observation by optical, fluorescence microscopes. RESULTS Exposed to toxin A, the cell growth inhibition, apoptosis index, non adherent cells and membrane damage in TPC 1 cell line were much more great than that in vero cell line, and the effect was dependent upon the concentration and treating time. CONCLUSION The antitumor activity of toxin A on TPC 1 cells was much higher than that on vero cell line. The data are of potential importance for the development of toxin A and the exploration of antitumor drugs.

OBJECTIVE To investigate the strategy of information system in antibiotics usage management. METHODS By inquisition,statistic and analytic methods the antibiotics usage information of the hospitalized patients was recorded with the information system,to realize the realtime monitoring and process management. RESULTS The usage rate of antibiotics decreased from 88.79% to 80.19% and the prophylactic usage rate of the third cephalosporin decreased from 72.34% to 11.25%.The rational usage rate increased from 57.89% to 80.18%. CONCLUSIONS The management method of antibiotics usage with information system achieves obvious effect.