Cestode Infections ; parasitology ; Humans ; Infant ; Male

Objective To explore the clinical and pathological features, treatment, and prognosis of capillary proliferative purpura nephritis (DEP-HSPN) in children.MethodsThe clinical data of 19 children diagnosed with DEP-HSPN were retrospectively analysis. Fifty-five children diagnosed with HSPN by renal biopsy were randomly selected as control group. ResultsThe average age was 10.6±2.6 years old, and the average course of disease were 19.4±7.4 days before renal biopsy in 19 children with DEP-HSPN (14 males and 5 females) who make up 3.92% of anaphylactic purpura nephritis children conifrmed by renal biopsy in the same period. In these 19 children, there were 10 cases having nephrotic syndrome and 9 case having hematuria and proteinuria type, all of whom were received immunosuppressive therapy. Finally, 14 cases achieved completely remission and 5 cases had partly remission. All of their classiifcations of renal pathology wereⅢb levels, accompanied with 6.38% to 36.36% of crescents. Compared with 55 age and sex matched children with renal pathology classiifcation ofⅢb, the DEP-HSPN children had shorter disease course, higher level of proteinuria, and lower pathological score of chronic renal injury (P all?

Objective To investigate the effect of improving immunity in the treatment of chronic brucellosis,and to analyze and evaluate its clinical curative effect.Methods A patient with chronic brucellosis was treated with Mongolian medicine combined with chemical drugs to enhance immunity.The clinical symptoms,serological antibodies,Brucella DNA and immune function were compared before and after treatment.The specific antibody against Brucella in serum was detected by tube agglutination test (SAT) and tiger red plate agglutination test (RBPT).Brucella DNA in serum and blood cells was detected by PCR,and the peripheral blood lymphocyte subsets were detected by flow cytometry.Immuno-luminescence technique was used to detect serum immunoglobulin and complement components.Results After treatment,the clinical symptoms such as cold back,fatigue,and joint pain disappeared completely,and the results of serum specific antibodies against Brucella were SAT 1 ∶ 50 (++)and RBPT (+) with no changes before and after treatment,and the results of cells and serum were both negative after treatment though the results of DNA detection of Brucella were cell positive and seronegative before treatment.The results of immunological function test showed that γδT cells decreased to 9.50％ after treatment compared to 14.00％ before treatment,and the percentage of monocytes and Treg cells were 5.59％ and 7.33％ after treatment,which were higher than 3.35％ and 4.72％ of before treatment,and the level of complement C3 was 0.79 g/L before treatment and 0.91 g/L after treatment that was returned to normal reference range (0.88 ～ 2.01 g/L).Conclusion The patients with chronic brucellosis can improve their clinical treatment by improving immunity.

OBJECTIVETo identify comparatively several commercial Chinese herbal medicines and their counterfeits.

METHODThe micromorphological characters were identified. The shape, surface, section and other characters of the medicinal materials were identified by using anatomical lens and scanning apparatus. Pictures were taken and saved.

RESULTMain micromorphological differences between several Chinese herbal medicine including Lonicera macranthoides, L. similis, Cuminum cyminum, Plantago asiatica, Cuscuta chinensis, Sinapis alba, Salvia miltiorrhiza and their counterfeits were identified.

CONCLUSIONThe reference for the authenticity identification of Chinese herbal medicine and helpful experiences for the research of the same subject were provided.

Cuminum ; anatomy & histology ; classification ; Cuscuta ; anatomy & histology ; classification ; Drugs, Chinese Herbal ; Plants, Medicinal ; anatomy & histology ; classification ; Quality Control ; Salvia miltiorrhiza ; anatomy & histology ; classification ; Sinapis ; anatomy & histology ; classification

Objective To observe the circadian rhythm of blood pressure and investigate the impact of Norvasc and Tanatril administrated at different time points based on the theory of time therapeutics in hypertensive patients. Methods The 24-hour ambulatory blood pressure was monitored in 130 inpatients and outpatients with grade 3 or 2 hypertension from May 2008 to November 2009. A total of 34 dipper hypertensive patients were grouped into dipper blood pressure group and 5 mg/d of Norvasc and Tanatril were taken by them at 6:00 AM. A total of 96 nondipper hypertensive patients were further divided into three subgroups after adjustment for age and gender: 5 mg/d of Norvasc and Tanatril were taken by group I (n = 30) at 6:00 AM; 5 mg/d of Tanatril at 6:00 AM and 5 mg/d of Norvasc at 18:00 PM by group Ⅱ (n = 32); 5 mg/d of Norvasc at 6:00 AM and 5 mg/d of Tanatrilat 18:00 PM by group Ⅲ(n=34).The 24-hour ambulatory blood pressure monitoring was performed again after four weeks treatment and 24-hour mean systolic blood pressure(24 hSBP),24-hour mean diastolic blood pressure(24 hDBP),daytime and nighttime mean systolic blood pressure(dSBP,nSBP)and daytime and nighttime mean diastolic blood pressure(dDBP,nDBP),were analyzed.Results The 24 hSBP,24 hDBP and dSBP,and dDBP were reduced from(154.3±5.6),(95.4±3.1),(158.7±6.5),(99.6±3.7)mmHg to(137.2±3.9),(82.5±2.7),(139.7±3.8),(85.2±3.5)mmHg,respectively,in dipper blood pressure group(t=2.124,2.356,2.278,2.449,all P<0.05).The 24 hSBP and 24 hDBP of the three groups in nondipper blood pressure were decreased from(154.4±6.1),(156.7±6.7),(156.6±5.2),(95.8±2.8),(94.9±3.8),(95.7±3.2)mmHg to (139.6±4.1),(134.5±4.6),(133.4±3.5),(83.5±4.2),(80.8±5.6),(81.6±4.7)mmHg,respectively(t=2.038,2.040,2.135,2.142,2.213,2.216,all P<0.05).dSBP and dDBP were decreased from(158.6±3.50),(158.4±5.6),(159.5±4.),(98.4±3.7),(99.6±3.7),(83.9±5.2)mmHg to(138.9±5.4),(136.7±4.1),(137.4±6.4),(85.8±5.3),(83.6±5.1),(83.9±5.2)mmHg,respectively(t=2.021,2.252,2.261,2.217,2.167,2.076,all P<0.05).nSBP and nDBP were decreased from(146.7±6.9),(149.8±3.9),(150.2±4.1),(93.7±4.2),(95.7±4.3),(93.4±3.3)mmHg to(133.7±4.6),(129.8±5.7),(127.6±2.8),(87.8±2.9),(78.5±6.4),(77.8±4.8)mmHg,respectively(t=1.798,2.032,2.014,1.864,2.157,2.166,all P<0.05).There were significant differences in nSBP and nDBP among all groups after treatment(F=2.32,2.17,all P<0.05),and the effect of the drugs was better in groug Ⅱ and Ⅲ than in group Ⅰ(q=3.17,4.03,3.32,4.19,all P<0.05),but there were no significant differences between group Ⅱ and Ⅲ(P>0.05).Conclusions Blood pressure can be controlled effectively by taking two antihypertensive medictions in the morning in hypertensive dipper patient but the blood pressure of nondipper hypertensive patients were able to be controlled more efficiently by taking the two antihypertensive medictions separately in the morning and at the evening compared with that taking the two drugs together in the morning.

OBJECTIVETo study the effect of ex vivo expansion on the adhesion activities of umbilical cord blood hematopoietic stem and progenitor cells (HSPC).

METHODSFresh UCB CD(34)(+) cells were cultured in a serum and stroma-free culture system. At day 7, day 10 and day 14, CD(34)(+) cells were re-selected from the expanded products. The expression of adhesion molecules (CAMs) such as VLA-4, VLA-5, LFA-1, ICAM-1, HCAM, L-selectin and PECAM-1, and the adhesion activity of the expanded CD(34)(+) cells were evaluated and compared with those of precultured fresh CD(34)(+) cells.

RESULTS(1) The CD(34)(+) cells expressing homing-related CAMs were increased (from 15-fold increase for CD(34)(+) CD(54)(+) subset to 72-fold increase for CD(34)(+) CD(49e)(+) subset at day 14). (2) The expressions of CD(49d), CD(44), CD(11a) and CD(49e) on the expanded CD(34)(+) cells were increased or sustained the same levels as those on fresh UCB CD(34)(+) cells, while the expression of CD(62L), CD(54) and CD(31) on expanded CD(34)(+) cells declined with the cultivating. (3) Spontaneous adhesion and SDF-1-induced adhesion tended to be increased in the course of the first 10 day's culture.

CONCLUSIONSThe culture system used in this study could substantially support the expansion of HSPCs expressing the above CAMs, and the expanded HSPCs would sustain their intrinsic adhesion potentials.

Antigens, CD ; analysis ; Antigens, CD34 ; analysis ; Cell Adhesion ; Cell Adhesion Molecules ; biosynthesis ; Cell Division ; Fetal Blood ; cytology ; immunology ; metabolism ; Flow Cytometry ; Hematopoietic Stem Cells ; cytology ; immunology ; metabolism ; Humans ; Receptors, Lymphocyte Homing ; biosynthesis

OBJECTIVETo study the distribution of prevalence on hypercholesterolemia and borderline high cholesterol of Chinese residents aged 20 years and above.

METHODSTo detect the total fasting plasma cholesterol in 48 299 subjects among 20 year-olds and above.

RESULTSWithin a certain age range, the prevalence of both hypercholesterolemia and borderline high cholesterol increased with age, but there was a decreasing trend after 60-years of age in some regions. The prevalence of hypercholesterolemia increased to 12.53% in over 70 year-olds group from 1.36% in urban 20-year-olds, and increased to 5.15% from 60-year-olds group comparing to 0.98% of the 20-year-old group in rural areas. When comparing to the 20-year-old age group, the prevalence of borderline high cholesterol of the 70 age group in urban and rural increased by 4.4 and 4.2 times, respectively. The prevalence of hypercholesterolemia in each age group in urban were higher than that of in rural areas. In Rural I, it was higher than that of in Rural II , Rural III and Rural IV. The prevalence rates of both hypercholesterolemia and borderline high cholesterol of the males in the less than 50 year-olds group were higher than that in females, which was quite different from the group older than 50 years of age. As to borderline high cholesterol, similar result was also noticed.

CONCLUSIONWithin a certain age range, there was a trend that the prevalence of both hypercholesterolemia and borderline high cholesterol increased with age and related to regions which were classified by economic status. A distinct difference between males and females also existed.

Adult ; Age Factors ; Aged ; Asian Continental Ancestry Group ; China ; epidemiology ; Cholesterol ; blood ; Female ; Humans ; Hypercholesterolemia ; epidemiology ; Male ; Middle Aged ; Prevalence ; Rural Population ; Sex Factors ; Urban Population ; Young Adult

Objective To investigate the relationship of ultrasonographic features and their pathologic basics in women of child-bearing period with abnormal uterine bleeding, and to explore the diagnostic ideas in detecting endometrial lesions. Methods A total of 149 women in childbearing age who had endometrial lesions with pathologically confirmed endometrial lesions, including 88 cases of endometrial hyperplasia, 41 cases of endometrial polyp, 20 cases of endometrial carcinoma, at Fuxing Hospital, Capital Medical University from October 2001 to September 2016 were selected. Ultrasound exams were performed on patients in early proliferative phase of menstrual cycle or within three days after vaginal bleeding stopped. We recorded patients′ age, measured sum diameters of length, width and thickness of uterine, mean thickness of endometrium and maximum diameter of polypoid lesions. We grouped the patients by pathologic results and compared ultrasound parameters between groups. The thickness of endometrium over 1.0 cm was considered as ″increased″. The pathologic exams also included ovarian structures. Results (1) There was no significant difference in the average age of patients with endometrial hyperplasia, endometrial polyps and endometrial cancer (P>0.05). (2) Ultrasonography: there was no statistically significant difference in the sum of the diameters of the uterus in the three groups (P>0.05); the thicker diameter of the uterine cavity in the intimal hyperplasia group was compared with the endometrial polyp group and the endometrial cancer group [(11.70±5.42)mm vs(10.59±3.83)mm vs(17.14±8.70)mm],the difference was statistically significant (F=9.779, P < 0.001), endometrial hyperplasia and endometrial cancer, endometrial polyps compared with the endometrial cancer group, the difference in uterine cavity echo thickness was statistically significant (both P<0.001); there was no significant difference in the uterine cavity echo thick diameter between the intimal hyperplasia group and the endometrial polyp group (P > 0.05). Of the 88 cases of hyperplasia of intimal hyperplasia, 53 were polypoid, and the mean diameter of the polypoid uplift was compared with the endometrial polyp group[(17.13±10.45)mm vs(12.14±4.67)mm],the difference was statistically significant (t=3.112, P=0.002). The proportions of point and strip blood flow signals in 41 cases of endometrial polyps and 53 cases of polypoid hyperplasia were 75.61% (31/41), 41.51% (22/ 53). Of the 163 patients in the three groups, 63 patients had a uterine cavity echo of <10 mm (42.3%). (3) Pathological examination: 20 cases of endometrial cancer were endometrioid carcinomas, 11 cases of hysterectomies, 7 cases of well-differentiated endometrioid carcinoma, 3 cases of moderate differentiation, 1 case of poorly differentiated; at the same time, 8 cases of ovariectomy. In 7 cases, there were multiple atresia follicles, and one case was pure cyst. Of the 88 patients with endometrial hyperplasia, 77 were hyperplasia and hyperplasia, 1 had dysplasia, 11 had complicated hyperplasia, and 3 had dysplasia. Of the 41 cases of endometrial polyps, 33 were non-functional polyps, 3 were functional polyps, and in 4 cases of adenoid fibroid polyps, there was 1 case of canceration. Conclusion In the early stage of endometrial proliferation or within 3 days after vaginal bleeding cases, the uterine cavity echo thickness diameter ≥ 10 mm suggests abnormal endometrium; uterine cavity echo thickness diameter <10 mm, endometrial abnormalities cannot be excluded; dynamic monitoring of ovulation by ultrasound may indicate the presence or absence of risk factors for abnormal growth of the endometrium.

Objective To investigate the perception and willingness of patients on individualized medication of voriconazole,to discuss the factors influencing patients' decision-making,and to support the development of the practice guideline for individualized medication of voriconazole.Methods A cross-sectional study involving various types of patients in 9 hospitals was conducted by questionnaire to evaluate the perception and willingness on therapeutic drug monitoring (TDM) of voriconazole and CYP2C19 genotype testing.Results A total of 119 patients were involved,including 24 (20.17％) children.Ninety five (79.83 ％) patients thought voriconazole TDM was necessary during the treatment of fungal infections.There was a significant difference on preference towards voriconazole TDM between patients who had received voriconazole TDM,before and patients who had never taken voriconazole TDM (100％ vs 75.76％,P ＜ 0.05).Fifty four patients (45.38％) agreed on CYP2C19 gene test.For this question,there was a significant difference among patients with different types of medicare (P ＜ 0.05).The ranking of factors that contributed to the 2 decision-making was consistent (P ＜0.05).The clinical efficacy was the most important factor (mean rank 3.46 and 3.87,respectively),while cost (mean rank 1.89) and potential benefit (mean rank 2) was the least important factor for the 2 decision-making,respectively.Conclusion There was a significant variance among different types of patients on preferences towards voriconazole TDM and CYP2C19 genotype testing,which should be considered for clinicians to make clinical decisions.The perception and willingness of patients should also be considered during the development of clinical practice guidelines and clinical practice.

This study is to investigate the protective effects of the SB203580 against radiation induced mortality and intestinal injury of mice. A total of 67 male C57BL/6 mice (20.0-22.0 g) were matched according to body weight and randomly assigned to one of three groups: control, total body irradiation exposure (IR, 7.2 Gy) only, and IR (7.2 Gy) + SB203580 (15 mg x kg(-1)). 30 days survival rate was observed in the experiment. In intestinal injury experiment, the expression levels of caspase-3, Ki67, p53 and p-p38 were assayed in the mice intestine crypts. The results showed that the 30 days survival rate was 100% (control), 0 (IR) and 40% (IR+ SB203580), separately. Compared to the IR groups, the positive cells of caspase-3, p53 and p-p38 in crypt cells decreased 33.00%, 21.78% and 34.63%, respectively. The rate of positive cells of Ki67 increased 37.96%. Significant difference was found between all of them (P < 0.01). SB203580 potently protected against radiation-induced lethal and intestinal injury in mice, and it may be a potential radio protector.
Animals ; Apoptosis ; radiation effects ; Caspase 3 ; metabolism ; Enzyme Inhibitors ; pharmacology ; Imidazoles ; pharmacology ; Intestines ; drug effects ; metabolism ; pathology ; Ki-67 Antigen ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Pyridines ; pharmacology ; Radiation Injuries, Experimental ; metabolism ; mortality ; pathology ; Radiation-Protective Agents ; pharmacology ; Random Allocation ; Tumor Suppressor Protein p53 ; metabolism ; Whole-Body Irradiation ; p38 Mitogen-Activated Protein Kinases ; metabolism