Objective To study the effects of treating cholecystotomy syndrome with LiDan decoction. Methods All patients were recruited into a control group and a treatment group, with 50 patients in each group. The treatment group was taken with LiDan decoction orally, and the control group was treated with conventional western medicines. Results The total effective rate of the treatment group and the control group was 92.0% and 80.0% respectively, showing significant difference. Conclusion It was effective to treat cholecystotomy syndrome With LiDan decoction.

Objective To investigate the application value of B-ultrasound guided liver puncture biopsy in antiviral therapy for chronic hepatitis B. Methods 20 cases with chronic hepatitis B proposed antiretroviral treatment got liver biopsy for grading the degree,and had anti-viral treatment.We had analysis before and after treatment.Results Pathology results showed that grading of inflammation and fibrosis in varying degrees improved.Comparison of before and after treatment had statistical significance(P

DNA ; chemistry ; Genes ; Genes, erbB-2 ; Humans ; Pathology ; trends

OBJECTIVE: To prove that the neural stem cell can be separated from neural tissue in adult mammalian. Stem cells can separate and proliferate continually, and can divide further into neuron and glial cell. The uptodate researches of neural stem cells suggest a prospective approach to treat neurological diseases, i.e. the dysfunctional cells may be replaced with neural stem cells by transplantation for functional reconstruction. Therefore,many kinds of directional differentiative signal controlling of neural stem cells to differentiate at a certain direction.DATA SOURCES: Using the key term neural stem cell, differentiation,signal and cell transplantation, we searched the MEDLINE database plus the relevant articles in English language from January 1995 to August 2004. Meanwhile, using the key terms mentioned above, we searched Chinese journal full-text database, Wanfang database in Chinese language from January 1995 to August 2004.STUDY SELECTION: The articles on extracellular signal of the differentiation of neural stem cell with full summarization were selected.DATA EXTRACTION: A total of 30 articles were eligible, and the relevant contents were compared and summarized. Eighteen articles were selected at last.DATA SYNTHESIS: All 18 articles contained different aspects of signal regulation of neural stem cell, including various key cellular factors and autogene regulation.CONCLUSION: Both cellular signals in vivo and in vitro can affect the cell differentiation. The vital problem to be solved is how to induce neural stem cells to differentiate at a certain direction so as to realize the aim of transplantation.

Objective: To investigate the effect of different concentrations of dextrose transfusion on cerebral glucose metabolism in pediatric neurosurgical operations. Methods: Sixty anesthetized children undergoing selective cerebral tumor resection were randomly divided into three groups: Ringer lactate solution group (A, n = 20), 2.5% dextrose lactated Ringer's solution group(B, n = 20), 1% dextrose lactated Ringer's solution group (C, n = 20). All the hemodynamic data were recorded during anesthesia. The values of blood glucose and lactic acid were collected before intubation(T_a), 2 hours after opening cerebral dura mater (T_b) and the end of operation (T_c). The catheters for microdialysis were punctured into normal brain tissue. The contents of glucose and lactic acid were determined in the collected intercellular fluid by biochemical analysis instruments at T_A and T_B respectively. Results: There were no differences in heart rate(HR), mean arterial pressure(MAP) and arterial oxygen saturation (SpO_2) among three groups(P > 0.05). There were no differences in blood glucose at T_a between three groups(P > 0.05). The values of blood glucose at T_b and T_c were B group＞C group＞A group(P < 0.05). The values of blood glucose were higher at T_b and T_c than that of T_a in three groups(P < 0.05). There were no differences in blood lactic acid among three groups(P > 0.05). There were no significant differences in values of glucose and lactic acid in the collected intercellular fluid among three groups(P > 0.05). Conclusion: Transfusion of Ringer lactate solution or low concentration dextrose solution will increase blood glucose in pediatric neurosurgical operations, but still in the normal range, which has a little influence on blood lactic acid, glucose and lactic acid in brain intercellular fluid. It is a safe choice during neurosurgical operation.

Objective To investigate the effects of C-peptide on apoptosis induced by high glucose and analyze the relationship between the apoptosis and expression of eNOS in HUVEC. Methods We determined the HUVEC apoptosis by Annexin-V, the expression of eNOS mRNA of HUVEC by RT-PCR, the apoptotic quantity of HUVEC by DNA fragment ELISA. Results High level of glucose could increase HUVEC apoptosis (P<0.01) and reduce the expression of eNOS. After adding physiological or high concentration of C-peptide, the apoptosis decreased and the expressions of eNOS increased markedly, with the relationship coefficient of -0.854 (P<0.01). Conclusion High glucose may induce apoptosis. Physiological or high concentration of C-peptide may reduce the apoptosis induced by hyperglycemia, the relationship between apoptosis and eNOS is negative.

@@

Objective To analyze the genotypes and the variation trend of rubella virus strains circulating in Beijing and to provide a scientific guidance for the prevention and control of rubella.Methods The viral nucleic acids were extracted from diagnostic pharyngeal swab specimens collected by Beijing rubella laboratory.The 739 nucleotides of rubella E1 gene were amplified by reverse transcription-polymerase chain reaction ( RT-PCR) .The amplified products were sequenced and analyzed.Genotypes of the rubella virus strains circulating in Beijing and variations in their nucleotides and amino acids were analyzed by comparing with those of the WHO recommended rubella virus reference strains.Results The target nucleotide se-quences of 10 rubella virus strains were obtained for molecular epidemiological study.All of the 10 strains belonged to 2B genotype and formed an independent branch according to the phylogenetic tree based on the 739 nucleotide fragments of E1 gene.Most of the nucleotide mutations occurred in the 10 strains were non-sense mutations.The amino acid sequences of the circulating strains were highly conservative.No significant mutations in the antigenic sites were identified in the rubella virus strains circulating in Beijing except that one amino acid mutation from Ser to Phe in the No.282 amino acid of E1 protein was identified in the hemag-glutination inhibition and neutralizing site of one strain.Conclusion This study indicated that the rubella virus genotype 2B epidemic strains were isolated in Beijing.

There is no unity in judgment of obesity at home and abroad.WHO considered that there should have different evaluation criteria from childhood below and above 10 years and recommended "weight for height" be used for children under the age of 10 and body mass index(BMI)for children aged 10 to 24 years old.International Obesity Task Organization(IOTF) recommended BMI be suitable for young children for judgment overweight and obesity.Both of them had the same BMI standards for overweight and obesity for 18-year-old,25 and 30 kg/m2,respectively.But the Working Group on Obesity of China(WGOC)developed a criterion which cut-offs in 18 years were lower than that of WHO and IOTF,24 and 28 kg/m2,respectively.The treatment of obesity in childhood was different from that in adults.Many methods used for adults such as surgical liposuction,slimming drugs,hunger therapies and fasting could not be appropriate for childhood.A combined treatment in childhood including behavior modification,diet adjustment and exercise was recognized domestically and internationally.

Objective To explore the role of tissue-type plasminogen activator(tPA) in neonatal rat with hypoxia-ischemia brain damage(HIBD).Methods Seven-day-old Wistar rat pups were used for the Vannucci model of HIBD.Reverse transcription-polymerase chain reaction(RT-PCR),terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL),double immunostaining and immunoblot analysis were adapted to determin the expression of tPA at acute phase after HIBD and neural cell apoptosis and the blood-brain-barrier(BBB) damage.Results Neonatal hypoxia-ischemia triggers persistent induction of the plasminogen system.The increase of tPA activity induced the degradation of laminin and occludin which would aggravate the BBB damage.The number of neural cell apoptosis after HIBD increased progressively with the reperfusion time.Conclusions The increase of tPA at the acute phase after HIBD can help clot to dissolve,while its extravascular proteolysis will induce cell apoptosis and BBB damage which will aggravate brain injury.