1.Effect of local infiltration anesthesia with different concentrations of ropivacaine on healing of incision in rats
Chinese Journal of Anesthesiology 2012;(12):1453-1456
Objective To evaluate the effect of local infiltration anesthesia with different concentrations of ropivacaine on the healing of incision in rats.Methods Seventy-five adult male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 5 groups (n =15 each):natural healing group (group C),normal saline group (group NS),0.20% ropivacaine group (group R0.20),0.50% ropivacaine group (group R0.50) and 0.75% ropivacaine group (group R075).0.20%,0.05% and 0.75% ropivacaine 1.0 ml/kg were injected subcutaneously 10 min before incision in R0.20,R0.50 and R0.75 groups,respectively,while the equal volume of normal saline was given instead in group NS.Five rats in each group were chosen for recording the healing time.Ten rats were taken in each group for recording the length of the incision 72 h after operation.The skin tissues around the incision were removed for determination of fibroblast counts under light microscope,IL-1β content (by ELISA) and the expression of collagen (by immunohistochemisty) and IL-1β mRNA (by RT-PCR).Results Compared with Cgroup,the healing time and length of the incision were significantly shortened,the fibroblast counts and expression of collagen were increased in R0.20 group,the healing time and length of the incision were shortened,the fibroblast counts and expression of collagen were increased,and the expression of IL-1β mRN A and IL-1β content were decreased in R0.50 group,and the healing time and length of the incision were prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in R0.75 group (P < 0.05).Compared with R0.20 group,the healing time and length of the incision were significantly shortened,the fibroblast counts and expression of collagen were increased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.50,the healing time and length of the incision were significantly prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.75 (P < 0.05).Compared with R0.50 group,the healing time and length of the incision were significantly prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.75 group (P < 0.05).Conclusion Local infiltration anesthesia with 0.20% and 0.50% ropivacaine can promote the healing of incision in rats,0.50% ropivacaine provides better efficacy than 0.20% ropivacaine,and 0.75 % ropivacaine can inhibit the healing process.
2.Effect of different wave lengths of low power laser on fracture healing
Ye MENG ; Minghao JIN ; Ruifeng GE
Chinese Journal of Tissue Engineering Research 2009;13(28):5421-5424
BACKGROUND: Low power laser irradiation has positive effects on fracture healing, including shortening the time of bone union and enhancement of bone formation. OBJECTIVE: To evaluate the effects of different wave lengths in low power laser on eedy fracture healing. DESIGN, TIME AND SETTING: Randomized, controlled, animal experiment. The study was performed at the Laser Center of Dankook University Medical Center between May 2005 and May 2006. MATERIALS: A total of 60 female English Hartley guinea pigs, 9 weeks old, weighing (300~30) g, were used for this experiment. They were randomly divided into three groups control, 632, and 830 nm groups (n=20). METHODS: The right femoral middle shaft fractures were made with a bone cutter and fixed with intramedullary nails (1.4 mm diameter K-wire). The frequencies of 632 nm and 830 nm semi-conductor laser machines were used. The laser irradiations were started 48 hours after operation vertically at the fracture sites and were applied every two days, at 100 J/cm<'2> of the same irradiation doses and 2.52 cm<'2> of the same irradiation areas in different irradiation groups. Finally, 46 guinea pigs survived, including 14 in control group, 16 in 632 nm group and 16 in 830 nm group. MAIN OUTCOME MEASURES: Guinea pigs were sacdficed at 3 and 6 weeks separately. The bone healing was assessed by the Modified Zorlu Scoring System, including gross, radiologic and histologic examinations. RESULTS: In the gross and radiologic findings, both 632 nm and 830 nm irradiation groups shown a significantly greater rate of callus formation at postoperatively 3 weeks compared to the control group (P< 0.01). Quantity of callus formation in 830 nm group was more than 632 nm group, but there was no significant difference (P > 0.05). The result of histological findings showed a significant increase of osteoblastic proliferation in two irradiation groups than the control group at 3 weeks postoperatively (P < 0.01). Enhancement of osteoblastic proliferation was more obvious in 830 nm group than in 632 nm group at 3 and 6 weeks, but there was no significant difference (P > 0.05).CONCLUSION: Both 632 nm and 830 nm wave lengths are considered to be optimal irradiation wave lengths for eady fracture healing. Moreover, 830 nm irradiation is better than 632 nm irradiation for eady fracture healing.
3.The application of aqua-walking in patients with spinal cord injury
Chaoqun YE ; Shurong JI ; Ling MENG
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(04):-
Objective To determine the effect of walking in water on patients with spinal cord injury~SCI). Methods Twenty SCI patients were recruited in this study, and these patients were randomly divided into a control group ~10 cases) and an experiment group~10 cases).The patients of the experiment group were administered aqua-walking and conventional physical therapy~PT),and the patients of control group were administered the conventional PT only. Results After 10 weeks of therapy, the muscle tone, motor index score and functional ambulation classification of experiment group were ameliorated significantly in comparison with the control group(P
4.Study of senile escaping phenomenon in prostatic tissues
Tiejun PAN ; Xiangbao MENG ; Zhangqun YE
Chinese Journal of Urology 2006;0(S1):-
Objective To study the mechanism of senile escaping of benign prostatic hyperplasia(BPH) condition. Methods With polymerase chain reaction based telomeric repeat amplification protocol assay(TRAP),telomerase activity was examined in 13 normal prostate tissues,35 benign prostatic hyperplasia tissues,33 hyperplastic tubercle and diaphragm. Results Telomerase activity was detected in 2 of 13(15%)normal prostate tissues,17 of 35(49%)benign hyperplasia tissues,14 of 33(42%)hyperplastic tubercle tissues and 1 of 33(3%)diaphragm tissues.The positive rate of telomerase in BPH is higher than normal prostate (P
5.Effect of mistletoe lectins on proliferation and apoptosis in HT-29 colon cancer cell
Shaomin WANG ; Meng YE ; Shuming NI
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(09):-
AIM:To study the effect of mistletoe lectins on cell proliferation and apoptosis in HT-29 human colon cancer cell.METHODS:The effect of mistletoe lectins on cell viability in HT-29 cell was evaluated by MTT assay.Cell apoptosis was observed by DNA ladder analysis and Tunel.RESULTS:Mistletoe lectins(eg.,1-4 mg/L) inhibited cell growth in a dose and time dependent way in HT-29 cells.HT-29 cells apoptosis can be induced when exposed to mistletoe lectins(eg.,1-4 mg/L).CONCLUSION:Mistletoe lectins can inhibits proliferation and induces apoptosis in HT-29 cells in vitro.
6.Preparation and Clinical Use of Compound Weifu Emulsion
Ye LI ; Fangzhen MENG ; Yu LIU
China Pharmacy 2001;0(08):-
OBJECTIVE:To prepare compound Weifu emulsion and to observe its therapeutic effect on infantile eczema METHODS:The emulsion was prepared with a formulation consisting of Scutellaria,Chinese Angelica,Chuanxiong and dimethyl silicone oil etc and the quality control method was established RESULTS & CONCLUSION:This preparation is stable in quality and feasible in quality control;The effective rate in treating infantile eczema has reached 90% and no obvious adverse reactions were found This emulsion is worthy of recommendation for clinical use
7.Mutation analysis of dystrophin gene and clinical evaluation of phenotypes in X-linked dilated cardiomyopathy.
Jian LIU ; Ye TIAN ; Fanchao MENG
Chinese Journal of Practical Internal Medicine 2003;0(01):-
T is the etiologic mutation in this family.Screening of female carrier state and follow-up study of the confirmed carriers are important for X-liked DCM family.
9.Case of anal pain caused by lumbar disc herniation.
Tian YE ; Chu MENG ; Shan-shan WANG
Chinese Acupuncture & Moxibustion 2014;34(4):346-346
10.A clinical comparative study on fiber post-retained and metal post-retained restorations of 176 cases
Lianmei YE ; Ping LIN ; Yukun MENG
Chongqing Medicine 2015;(32):4512-4513,4516
Objective To compare the clinical effects of fiber post‐retained and metal post‐retained restorations .Methods Two hundred teeth after root canal therapy from 176 patients were divided into 2 groups randomly and each group consisted of 100 teeth .In the experimental group ,teeth were restored with fiber post‐resin core and porcelain crown ,where cast metal‐core and porcelain crown were used in the control group .The teeth were clinically examined regularly for 2 years .The data were analyzed with Chi‐square method .Results At the end of the 2‐year follow‐up ,6 fiber posts dislocated and 2 teeth showed gingival inflammation in the experimental group ,with a clinical success rate of 92% ,the loss rate of 6% .5 gingival inflammation cases ,2 periapical inflammation cases and 7 cases of root fracture occurred in the metal post group ,and the clinical success rate was 85% ,root discount rate of 7% .There was statistical significance between the two groups (P< 0 .05) .Conclusion The loss rate ogf fiber post‐retained restoration is higher than the metal post‐retained restorations ,but the root discount rate is low ,incidence of periodontal and periapical tissue similar in the two groups can be routinely used clinically instead of metal post‐retained restoration .