China ; Hepatectomy ; methods ; trends ; Humans

Objective To investigate the change of immunologic function of red blood cell (RBC) in patients with chronic gastritis. Methods The immunologic function of RBC in 88 cases of chronic gastritis was dynamically detected with yeast rosette method. Results The results showed that RBC-C3b receptor rosette (RBC-C3b-RR) rate and RBC immune adherence enhance factor (RFER) rate were significantly lower (P＜0.05) and RBC immune complex rosette (RBC-ICR) rate and RBC immune adherence inhibitor factor (RFIR) were significantly higher (P＜0.05) in the patients with atrophic gastritis than those in control group. In the patients with superficial gastritis, the difference had no distinct significance (P＞0.05). Conclusion The immunologic function of red blood cell (RBC)measurement plays an important role in differential diagnosis and prognosis evaluation of chronic gastritis.

OBJECTIVE:To establish an HPLC method for determination of ganciclovir in serum of dogs and to study pharmacokinetics and relative bioavailability of Ganciclovir dispersible tablets.METHODS:The determination was performed on Dikma DiamonsilTM C18 column.The mobile phase consisted of 0.015 mol?mL-1 monobasic potassium phosphate buffer solution (pH 2.50,containing 0.25% acetonitrile) with flow rate of 1.75 mL?min-1 at detection wavelength of 254 nm.The column temperature was set at 40 ℃.In a randomized cross-over study,a single dose of dispersible tablets and the capsule were given to 6 dogs.An HPLC method was used to determine plasma concentration of ganciclovir in dogs and the pharmacokinetic data were treated by DASver1.0 software.RESULTS:The linear range of ganciclovir were 0.050 25～100.5 ?g?mL-1;the methodological recovery was larger than 90% (RSD

Objective To explore the risk factors of vascular dementia (VD) in stroke patients, expecial-ly, the relationship between VD and carotid artery atherosclerosis. Methods 64 patients with cerebrovascular dis-ease , who had a history of stroke , were divided into VD groups (n = 64) and control group (n = 60). The age, disease course, education and blood lipid and carotid artery stenosis were compared. Results The age of patients , course of disease ,educational levels, the serum levels of TC ,TG, LDL and internal diameter of carotid artery were different between the two groups (P < 0.05 ). Conclusion The age of patients , course of disease , education, the serum levels of TC ,TG ,LDL and carotid artery atherosclerosis are related to vascular dementia in stroke patients.

Objective:To research expression and function of c-myc gene in hyperacute r ejection of xenotransplantation. 　　Method:Through the model of hyperacute rejection of xenotransplantation wit h isolated rat heart perfused with human plasma,we detected c-myc mRNA expressi on and cell localization with in situ hybridization in hyperacute rejection. 　　Result:c-myc mRNA increased expression in the endothelium cell of heart.Th e value of signal is 450.09±409.99.The value of signal of group of control is 1 74.40±51.50(p＜0.05)。 　　Conclusion:From these study findings,it would appear that hyperacute rejecti on is associated with the elevation of c-myc mRNA expression.

Objective: To establish the database on typing of Chin es e condyle head by skull base position X ray analysis. Methods: 51 Chinese volunteers (male 33, 65 condyles; female 18, 35 condyles) accep ted skull base position X ray inspection. Then the 100 condyle images were ana lyzed and the contribution of the types among sexes and sides were also analyz ed. Results: The types of condyle were divided into A,B,C, D,E(E1,E2) and F(F1,F2).Among all condyle types, type A was accounted for 41.0% , while type F1 only 1%. The contribution of the types between left and right si des was almost the same. 51% (26 pairs) of the two condyles in the same person share the same type, the other 23 pairs of condyles were in different types (mal e 39.1 %, female 60.9%). Conclusion: The morphology typ ing of condyle heads by skull base position X ray analysis may be reference to clinical application.

Objective To explore the consistency of chemiluminescence microparticle immunoassay(CMIA)reagent and particle‐enhanced turbidimetric immunoassay(PETIA) reagent for detecting pepsinogen(PG) ,so as to provide laboratory references for en‐suring the reliability of PETIA reagent in detecting PG .Methods According to the requirement of EP9‐A2 file made by the Nation‐al Committee for Clinical Laboratory Standards(NCCLS) ,taking PETIA as evaluating method and CMIA as reference method ,a to‐tal of 92 specimens collected from patients with various degrees of gastric atrophy were selected ,PETIA and CMIA reagents were utilized for bidirectional detection of PG .Then recorded test results of pepsinogenⅠ(PGⅠ) ,and PGⅠ /pepsinogenⅡ(PG Ⅱ) rati‐o ,and carried out method comparison and bias evaluation for the two kinds of reagents .Results The expected relative bias of detec‐tion results of PGⅠ and PGⅠ /PG Ⅱ ratio by using PETIA and CMIA reagents were acceptable within linear range of methods . Conclusion The results of PGⅠ and PG Ⅱ detected by using PETIA reagent could be accepted .

Objective Previous studies have found that micheliolide(MCL) could improve the sensitivity of breast cancer cells to chemotherapeutic drugs such as cisplatin and induce the apoptosis of breast cancer cells.This article aims to study the proliferation inhibition effect of micheliolide on lung cancer cells H460 and its underlying mechanism.Methods Human lung cancer cell line H460 was treated with different concentrations of micheliolide(30,60,90μmol/L).Then the cell proliferation was measured by-CCK8 and plate colony formation assays.The apoptosis and the cell cycle were detected by flow cytometry.Western blot was used to determine the mechanism of how MCL affecting cancer cell H460.Results Compared with the control (275.00±7.21), the clone numbers after 30μmol/L,60μmol/L and 90μmol/L MCL treatment(199.00±5.66,166.00±1.41, 90.00±7.81) were significantly decreased (P<0.05).Meanwhile, the CCK-8 results showed that compared to the control group, A value was significantly increased after 30μmol/L MCL treatment for 72h and 96h, and 60μmol/L or 90μmol/L MCL treatment for 48h,72h and 96 h (P<0.05).Compared with the control apoptotic ratio [(2.90±0.03)％], the ratio of early and late apoptotic cells after 30μmol/L, 60μmol/L and 90μmol/L MCL treatment [(5.23±0.76)％, (9.06±0.47)％, (19.00±0.64)％] were significantly increased (P<0.05).Compared with the control group, the ratio of G2/M phase cells[(12.52±0.88) ％ ,(17.22±0.43)％, (19.84±0.31)％] was gradually increased after 30μmol/L, 60μmol/L and 90μmol/L MCL treatment, and there was statistically significant difference after 60μmol/L and 90μmol/L MCL treatment (P<0.05).The ratio of S+G1/G0 phase cells[(87.53±1.06)％ ,(82.94±0.67)％ ,(79.79±0.21)％] was gradually decreased after 30μmol/L, 60μmol/L and 90μmol/L MCL treatment, and there was statistically significant difference after 60μmol/L and 90μmol/L MCL treatment (P<0.05).The expression level of notch4 was significantly decreased after 30μmol/L, 60μmol/L and 90μmol/L MCL treatment (P<0.05), while the expression level of cleaved caspase3 was significantly upregulated (P<0.05).Conclusion MCL exerted an inhibitory effect on lung cancer cell H460.

Objective To study the expression of hemeoxygenase-1 (HO-1) and change of superoxide dismutase (SOD) activity in perihematoma after intracerebral hemorrhage (ICH). Methods The rats were randomly divided into sham operating group and intracerebral hemorrhage group.Rat ICH models were induced using stereotactic infusion autologous blood 50 ?l into the caudate nucleus. At the due time, the rats were killed and brain tissues were removed for detection of HO-1 and measurement of SOD by immunohistochemical and xanthine hydrocarbonylation methods. Results Following ICH, few HO-1 positive cells were observed in brain tissue perihemota at 6 h, peaked at 48 h ( P0.05), then decreased gradually and reached the minimum at 72 h ( P0.05). The change of SOD in brain tissue perihematoma was negatively correlated with the expression of HO-1 (r=-0.878, P

Objective To investigate the effect of medial collateral ligament reconstruction using semitendinosus autograft.Methods A total of 12 patients with medial collateral ligament injury received knee examinations.Among them,2 had lateral meniscus injury,and received partial resection of the lateral meniscus;2 had cruciate ligament(CL) injury(anterior CL in one and posterior CL in the other),and underwent bone-ligament-bone reconstruction.Medial collateral ligament reconstruction was performed using the semitendinosus tendon autograft ingrowth in the tibial tunnel.Results Primary healing was achieved in all the cases.In 10 of them,the stability of the knee was regained in 6-month follow-up;stress X-ray examination showed that the medial joint space was smaller than the contralateral one by less than 5 mm,and the position of the interference screw was optimal.Of the 12 patients,11 achieved an active knee flexion between 0? and 120?,the other,who had fracture of the lateral condyle of the femur,had a flexion of 90?.Conclusions Semitendinosus autograft provides sufficient tension for medial collateral ligament reconstruction.The fixation of the knee and the closure of the joint capsule can be achieved by using the method.