Objective:To investigate the effects of Yizhi Oral Solution on protein and RNA synthesis of brain tissue of mice. Methods: The radioactive intensities of 3H leucine in brain protein and 3H uridine in RNA of brain tissue of young mice were observed by the methods of incorporation in vivo and group control observation.Results: The radioaetive intensity of 3H leucine of Yizhi Oral Solution group was higher than that of cycloheximide pathological model group ( P

In order to observe the febrifugal response of needling "Chang Qiang Point", and analyse its mechanism lumbar spinal cord of rabbits were damaged in the expermental study. The experimental findings were as follows: 1. It was found that cAMP content in csf was striking increased, when leukocytic pyrogen fever of rabbits was significant. During needling "Chang Qiang Point" of febrile rabbits, however, febrile response was markedly inhibited(at 60 min~?T℃ was 0.23?0.07 and TRI4 0.61?0.03). There was significant difference(P0.05). 3. After damaging lumbar spinal cord of rabbits, there were certain change of rectal temperature and the febrile response, but it had no effect on cAMP content in csf. The authors infered that the impulses from "Chang Qiang Point" transmiled Via spinal cord might be closely associated with reflex mechanism and the change of cAMP content might play an important role in the mechanism of febrifugal effect during needling "Chang Qiang Point" in febrile rabbits.

Objective To analyze the pressure source and effect of comprehensive intervention in type 2 diabetes mellitus patients. Methods 200 diabetes patients were randomized into the observation group and the control group with 100 cases in each group. Comprehensive intervention was introduced into the observation group and the control group underwent routine treatment. The pressure source was investigated after intervention by questionnaires and the results were compared. Results Fearing of serious complications bocame the most common cause of pressure. The scores of Self-rating Depression Scale(SDS)and Self-rating Anxiety Scale(SAS)were lower and the biochemical index was also better in the observation group than those in the control group as well as those before intervention in the observation group(P＜0.05).Conclusion The depression, anxiety status and biochemical index in patients with type 2 diabetes mellitus were effectively controled by corresponding and comprehensive intervention measures according to the sequence order of pressure source.

Objective　To study the clinico-pathological features of primary adrenal lymphoma (PAL).　Methods　2 cases of PAL were reviewed and studied.　Results　The age of the 2 patients was 45 and 57.The presenting symptoms were nonspecific.Histologically,the neoplasm was composed of numerous medium-sized cells with round nucleus,usually 1-2 in number and with relatively abundant basophilic cytoplasm.The neoplastic cells showed frequent mitosis.Immunohistochemically,the tumor cells were strongly positive for LCA,L26 and CD74 while negative for UCHL-1,S-100 protein and CK. One case was discharged 3 months after operation because of dyscrasia and the other was lost on follow up.Prognosis of PAL was poor.　Conclusions　PAL is extremely rare with a high grade of malignancy.The diagnosis could be made on histopathological studies and immunohistochemical studies.

Objective To preparing the reference material(RM) of lactate dehydrogenase(LD),we purification of LD from human erythrocyte(RBC)and studied its properties.Methods Using a modified procedure which including complete hemolysis of the RBC,hydroxyapatite treatment,(NH4)2 SO 4 Precipitation,CM- Sephadex C 50,Sephadex G100 and 5'- AMP- Sepharose 4B affinity chromatography.Results The purified LD has a sepecific activity of 163.0 kU/g protein.It is almost free of contaminating enzymes.Two corresponding bands were observed on both PAG plates stained for either protein or LD activity after electrophresis had been done.The apparent Michaelis constants were of 1.000 and 0.179 mmol/L for L-lactate and NAD and of 0.119 mmol/L 0.062 mmol/L for pyruvate and NADH respectively.Conclusion The final purified LD was found to be very similar to that in human serum in catalytic properties.It is intended to be a fundament to prepare a RM for the measurement of LD.

ISO 15189 standard quality management system developed by the International Organi-zation for Standardization (ISO) embodies the international medical laboratory's latest quality management concept, and puts forward the quality improvement method of the standard. In this research, ISO 15189, as an important content in practice teaching for laboratory medicine, was designed according to the elements of this system. Students were requested to participate in every linkage of laboratory quality management by writing system document, taking part in quality activity, and practicing the key and difficult points of tech-nique in quality system in order not only to cultivate their basic idea of ISO 15189 standard, but also to make them familiar with the basic patterns of laboratory accreditation system and process, which could make them become the backbone of the laboratory quality management quickly in their vocational positions in the future.

OBJECTIVE:To study the potential targets of Guizhi decoction regulating Ying and Wei based oncorrespondence between formulation and syndromerelationship and reverse molecular docking technology. METHODS:Active ingredients related to Guizhi decoction were obtained by literature retrievals,and PharmMapper Server was used for reverse molecular docking for ac-tive ingredients. The potential receptors of Guizhi decoction were found on the basis of docking scores,then AutoDock Vina was conducted for positive molecular docking test to observe the affinity between the ligand and the receptor molecule. 75 rats were ran-domly divided into normal group,model group,Guizhi decoction high-dose,medium-dose,low-dose groups(12,8,4 g/kg),15 in each group. Except for normal group,rats in other groups were induced for models with disharmony between Ying and Wei. Af-ter modeling,rats were intragastrically administrated once a day,for 5 d. After administration,11β-HSD1 expression levels in adi-pose tissue and muscle tissue of rats were detected. RESULTS:Reverse molecular docking for active ingredients of Guizhi decoc-tion found that 11β-HSD1 was the frequent receptor,and positive docking test confirmed that uralsaponin b,glycyrrhetnic acid and carbenoxolone and so on had higher affinity with 11β-HSD1. Results of animal test showed,compared with normal group,11β-HSD1 expression levels in adipose tissue and muscle tissue in model group were increased (P<0.05);and compared with model group,11β-HSD1 expression levels in adipose tissue and muscle tissue in Guizhi decoction high-dose group were decreased (P<0.05). CONCLUSIONS:Guizhi decoction regulating Ying and Wei has certain correlation with 11β-HSD1,while the reverse molec-ular docking technology can provide a feasible technical way to studycorrespondence between formulation and syndromerelation-ship.

This paper introduces the development of the MEMS at home and abroad,besides the key technology,the application in medicine and military about the MEMS also expounded in detail.

AIM:To evaluate the effect of Huazhuo Jiedu Huoxue Recipe(Herba Agastaches,Herba Eupatorii,Rhizoma Pinelliae,Pericarpium Citri reticulatae,Poria,etc.) on plasma orphanin FQ(OFQ) and prostaglandins in the patients of endometriosis(EM) with pain.METHODS:The concentrations of plasma OFQ and prostaglandins were measured by radioimmunoassay before and after the treatment on the patients of EM with pain.29 patients of EM with severe pain(severe group),31 patients with slight pain(slight group) and 25 patients without pain(control group) were studied.RESULTS:The levels of OFQ、 PGF_(2a)、 PGE_2、 6-keto-PGF_(1a) and TXB_2 in plasma were significantly higher in patients of EM with pain than that of patients without pain(P

Objective To investigate the influence of Zengshiling oral liquid on gastric emptying,plasma motilin (MOT) and small intestine propulsion of young rat. Methods Experiment animal were divided into five groups randomly:control group,large dose of Zengshiling oral liquid group,normal dose of Zengshiling oral liquid group,small dose of Zengshiling oral liquid group and Motilium group. The rats were gavaged by 0.9% of NaCl and corresponding drug. 7 days later,gastric emptying was assayed by phenol red,plasma MOT levels and carbon powder propelling rate of small intestine were also assayed. Results The rate of gastric emptying of Zengshiling oral liquid groups were significantly higher than control group (P