The early clinical symptoms of newborns with acute bilirubin encephalopathy are absent or slight.In addition ,this diseaes is no specificity.So ne wborns with acute bil rubin encephalopaht y is easy to miss ni d iagnosis.If the best treatmetn opportunity of this disease is delayed,it can affect the long-term prog-nosis and survival.Neuros-pecfi ic nlo ase,cranial magnetic resonance imga ing,electroencpe halogram,amlp i-tude integrated electroencephalogram and rb ainstem auditory evoked potential can judge the position and in-tensity of brain injury from the different angle and level,which can provide important basis for the early diag-nosis and intervention of acute bilirubin encephalopathy.

ObjectiveTo study the feasibility,safety and effect of laparoscopic splenectomy and selective pericardial devascularization in the treatment of portal hypertension.MethodsThis study included 7 patients diagnosed as posthepatitie cirrhosis and portal hypertension with a history of up GI bleeding receiving laparoscopic splenectomy and selective pericardial devascularization.ResultsThe operation was successful in all 7 patients without conversion to laparotomy.There were no postoperative severe complications.The average operation time was 4.2 h,mean blood loss was 430 ml.All patients recovered well,and were followed-up for 0.5 ～ 2.0 years,and there was no rebleeding in this period.ConclusionsLaparoscopic selective pericardial devascularization is feasible,safe,minimally invasive.The short to mid term result was satisfactory.

OBJECTIVE To investigate and know the site distribution,susceptible factors and antibiotic resistant condition in hospital-acquired infection by Enterococcus so as to provide reference for clinical preventive control and for reasonable uses of medicine.METHODS Sixty five Enterococcus strains isolated from different infection specimens in the hospital from 2001 to 2005 were analyzed,and examined their sensitivity to medicines.RESULTS Among 65 Enterococcus strains there were 36 strains of Enterococcus faecalis and 29 strains of E.faecium.The first three susceptible site diseases were alimentary,circulatory and injury.The site distributions were urinary tract,lower respiratory tract and surgical incisions.Related aggressive operations included urethra intubation,tracheal intubation,and surgery.Except for vancomycin and teicoplanin,E.faecalis was sensitive to ampicillin,high-level streptomycin,levofloxacin and nitrofurantoin,and E.faecium had antibacterial activity to chloramphenicol and tetracycline.There were 2 vancomycin-resistant Enterococcus(VRE) cases detected.CONCLUSIONS It is the easiest to examine and find Enterococcus in urine and the prevalence rate is high after related aggressive operations.The isolation rate of E.faecium is high and it has strong antibiotic resistance.Vancomycin is still the most sensitive medicine to Enterococcus.

Objective To investigate the clinical features,treatment and prognosis of paraneoplastic pemphigus (PNP).Methods A retrospective study was performed on 11 patients with PNP hospitalized in the Department of Dermatology,Ruijin Hospital.Clinical characteristics of these patients were analyzed.Results Of the 11 patients,10 had oral or labial erosions or ulcers,and 6 had obstructive bronchiolitis.Computed tomography (CT)showed solitary internal tumors in all the patients after appearance of skin lesions,and 8 of them were diagnosed with Castleman's disease.All the patients had been treated with corticosteroids before operation,but achieved no obvious improvement.After 2-7 months of postoperative treatment with low-dose prednisone and thalidomide,both cutaneous and mucosal lesions healed with the relief of pulmonary symptoms in 5 patients.Conclusions Oral erosions or ulcers appear to be the most common initial manifestation of PNP with Castleman's disease as the most frequent accompanying tumor.Early detection and timely resection of tumors are keys to successful treatment of PNP,and postoperative treatment with glucocorticoids and thalidomide proves to be effective for PNP.

Inflammatory bowel disease (IBD) is a group of chronic and non-specific intestinal inflammatory diseases of undetermined origin,which including ulcerative colitis (UC) and crohn's disease (CD).Interleukin(IL) is a kind of cytokines,and plays an vital role in growth,differentiation,immune response of immune cell.With a intensive study in the pathogenesis of IBD in the world,parts of IL were found and confirmed to be a important role in the progression of IBD,and will become a new hot spot in the research of the diagnosis and treatment of IBD.

Objective To research the clinical evaluation of potassium dehydroandrograpolide succinate injection in the treatment of COPD combined with CAP and its influence on plasma IL-33, PCT and hs-CRP levels.Method 91 patients with COPD combined with CAP in our hospital from April 2015 to August 2016 were selected and divided into the observation group (48 cases) and the control group (43 cases).The control group was given routine treatment, the observation group was given on the basis of the above treatment of potassium dehydroandrograpolide succinate injection.The clinical efficacy and plasma IL-33, PCT and hs-CRP levels were compared between the two groups after treatment.Results Before treatment, the IL-33, PCT, hs-CRP of two groups of patients had no significant difference.After treatment, the total effective rate of the observation group was 91.67% higher than that of the control group 74.42%, the difference was statistically significant (P<0.05).All the clinical symptoms time of the observation group was significantly lower than that of the control group, and the clearance rate of the observation group (85.42%) was significantly higher than that of the control group (67.44%), the difference was statistically significant (P<0.05), the indexes of the two groups of patients were decreased, the indexes of the observation group significantly lower than that in control group, the difference was statistically significant (P<0.05).There was no significant difference in the incidence of adverse reactions between the observation group and the control group .Conclusion Potassium dehydroandrograpolide succinate injection can significantly improve their plasma IL-33, PCT and hs-CRP levels, the respiratory pathogen and inflammatory mediators are effectively removed, and has good clinical effect, and no serious adverse reactions during treatment.

Objective To study the mechanism of senile escaping of benign prostatic hyperplasia(BPH) condition. Methods With polymerase chain reaction based telomeric repeat amplification protocol assay(TRAP),telomerase activity was examined in 13 normal prostate tissues,35 benign prostatic hyperplasia tissues,33 hyperplastic tubercle and diaphragm. Results Telomerase activity was detected in 2 of 13(15%)normal prostate tissues,17 of 35(49%)benign hyperplasia tissues,14 of 33(42%)hyperplastic tubercle tissues and 1 of 33(3%)diaphragm tissues.The positive rate of telomerase in BPH is higher than normal prostate (P

Objective To study the relationship between endothel in-1 in plasma and TCD in patients with brain injury.Methods We measured dynamically the level of ET-1 in plasma on 33 adult brain injured patients. Cerebral vasospasm was detected by transcranal Doppler sonography (TCD).Results 1.During 6-8 days, 13-15 days after injury,there was a significantly positive correlation between the level of ET-1 in plasma and cerebral vasospasm (r=0.533,P=0.002;r=0.423,P=0.02),especially during 6-8 days after injury. The increase of the level of ET-1 in plasma paralleled paroled with the time of cerebral vasospasm developed. 2.During 6-8 days after injury, the level of ET-1 in plasma of patients with cerebral vasospasm was obviously higher than those without cerebral vasospasm obviously (P

Objective An analytical approach has been studied lor high performance liquid chro-matographic determination of Diphacinone and its metabolites extracted from urine. Method Diphacinone and its metabolites extracted from rabbit urine by solid phase extraction (SPE) cartridges (CN and SAX) were analyzed with coumarin as the internal standard. Diphacinone and its metabolites were separated by reversed-phase gradient chromatography with DAD detector at 311nm. The analytical column was Hypersil BDS C18 (150mm?4.6nmi. d. ) and the guard column was Phenomenex ODS (4mm?3.0mmi. d.). The mobile phase was a gradient mixture of aqueous solution (A) and methanol solution (B), both containing 0.5% ion pair A. Results Eleven diphacinone metabolites were detected in poisoned rabbit urine by this method. Conclusion This method is simple, exact and rapid.

Objective To study the effects of diacylglycerol(DG)on body weight of rats and its possible metabolic mechanism. Method (1) SD rats (n=30) were randomly divided into 3 groups. They were given free access to diets containing 7% (wt) triacylglycerol (TG, control group), 20% (wt) TG or 20% (wt) DG diets, respectively for 8 w. The changes of body weight, height, food intake, and feces were recorded. At the end of experiment, abdominal fat weight (including perirenal fat and epididymal fat), blood lipids were detected. (2) 13 w male Wistar rats (n=50) were divided into 2 groups(administrated with 10% TG or DG emulsion, respectively)in postprandial blood fat profile experiment. Blood lipids of 5 rats were analyzed at desired interval. (3) 6 w male Wistar rats (n=30) were randomly divided into 3 group, control group, DG group and TG group, administrated with glucose solution, 20%DG emulsion and 20%TG emulsion respectively for 6 d. Urine in 144 hr was collected continuously and analyzed for total ketone bodies. Results High DG diet resulted in a significant reduction in both body weight gain, ratio of abdominal fat to body weight and serum TG levels compared with the high TG diet. DG group have higher FFA level in portal vein and lower TG level in jugular vein than those of high TG group. But urine ketone body level of high DG group was higher than high TG group. Conclusion Dietary DG reduced fat accumulation inabdominal region, body and blood, and these effects may be involved with different metabolic ways of DG compared with TG.