Objective To explore the pathogenesis and clinical value of Th1/Th2 and normal intestinal flora from the angle of immunological and intestinal micro environment balance by detecting the plasma interferon-γ(IFN-γ)and interleukin-4(IL-4)and fecal bifidobacteria in Children with Allergic Asthma,so as to provide a theoretical basis through early intervention of probiotics to correct the Th1/Th2 balance.Methods The levels of blood plasma IFN-γ,IL-4 and fecal bifidobacteria amount of 165 cases of chil-dren with allergic asthma and 150 health students as control group were detected by ELISA and real time fluorescence PCR at the same time.Retesting these above indexes after applying bifidobacteria regulation of intestinal flora for 6 months.The clinical relative factors and experimental data were statistically analyzed.Results The level of IFN-γof asthmatic children were remarkably lower than those in the control group(P <0.05),while the level of IL-4 of asthmatic children were remarkably higher than those in the control group(P <0.05),and the amount of bifidobacteria of asthmatic children were remarkably lower than those in the control group(P <0.05).The level of IFN-γand bifidobacteria of asthmatic children improved remarkably and the level of IL-4 decreased significantly after applying bifidobacteria regulation of intestinal flora for 6 months.All of them had remarkable statistically signifi-cant.Conclusion The immune balance was affected by the intestinal flora balance of vice versa.This would provide an important theo-retical support to prevent the occurrence of allergic asthma by the early probiotics regulation of intestinal flora and immune balance.

Objective: To evaluate the clinical effect of the double-orifice technique in the treatment of the anterior leaflet prolapse of mitral valve. Methods: 35 patients with severe anterior leaflet prolapse of mitral valve underwent valve repair using the double-orifice technique. The condition of valve was assessed with echocardiography preoperatively, intraoperatively, and postoperatively. Results: The mean postoperative valve area was (4 1?1 8) cm 2 against the preoperative valve area of (8 3?1 6)cm 2. Mild regurgitation was found in 2 patients after operation. There were no hospital deaths. At the latest follow-up, the heart function was improved significantly in all patients (NYHA class I). Echo-Doppler assessment of valve showed stable valve function in all patients. There were no late stenosis of valve and no late death. Conclusion: The results of this study show that the double-orifice technique is a safe and effective method for treatment of anterior leaflet of mitral valve with low mortality and morbidity, and excellent early and mid-term results.

Therapies for uveitis include drug treatment and surgery.Medical therapies including proper administration of corticos- teroids,immunosuppressive drugs,nonsteroidal anti-inflammtion drugs and anti-infective drugs are important in management of the in- traocular inflammation and decreasing the risk of complications,furthermore,are the basis for surgical treatment of uveitis.

Objective: To analyze the construct validity of performance evaluation system of clinical centers by exploratory factor analysis strategy, so as to provide evidence for optimizing the parameters of the evaluation system. Methods: Using the exploratory factor analysis method, we evaluated the construct validity of the constructed performance index system. Results: Factor analysis showed that the factor components (listed in an increasing importance order) were: discipline superiority, cultivation of talented persons, medical service, scientific research capability, and infrastructure construction, etc. (the cumulative contribution of the first 6 factors was 90.17%). Most of index factor loadings matched with the meaning of their corresponding factors, indicating that the construct validity of the evaluation system was consistent with the expectation of its designers. Conclusion: The constructed clinical center performance evaluation system has a reasonable factor structure.

The learning passion,apprehension and master of probation knowledge and practice capacity of probationers have improved greatly by applying the teaching method of stage-separated to echelon teaching on the basis of the cognitive law and focusing on clinical operation practice.Meanwhile,this method has effectively improved clinical probation teaching quality of orthopedics.

Objective To determine the expression of metastasis-associated gene 2 ( MTA2 ) and p53 in colorectal cancer and non-cancerous mucosa, analyze their relationship with clinicopathological parameters, and discuss the clinical significance. Methods 120 colorectal neoplasm patients' cancer tissues and clinical information were col-lected from the first affiliated hospital of Anhui medical university. 30 patients of them were chosen to collect non-cancerous mucosa which was 5 cm away from the tumor. Immunohistochemistry staining was used to detect the ex-pression of MTA2 and p53. According to the clinicopathological parameters, the positive and negative expression of MTA2, p53 were counted each group, discussed the relationship between positive expression and clinicopathologi-cal parameters by SPSS 19.0 . Results The immunohistochemistry showed that the positive expression rate of MTA2 was 59.16%, and the positive expression rate of p53 was 61.67%. The expression of MTA2 and p53 in colorectal cancer were significantly higher than non-cancerous mucosa ( P<0.05 ) . The MTA2 expression was positively cor-related with the depth of invasion, lymphatic metastasis, distant metastasis and TNM stages (P<0.05). The p53 protein expression was positively correlated with depth of invasion and lymphatic metastasis ( P<0.05 ) . Conclu-sion MTA2 could be used as a new clinical biomarker and therapeutic target for colorectal cancer probably, which might be more effective than p53 .

Objective: To investigate nutrition status and diet condition of 1 000 medical studients,and intervent their diet in order to improve the constitution.Methods: 200 practicing medical students' basal energy expenditure was firstly measured and their diet structure was investigated 1 000 practicing medical students were choosed and their diet kinds and calories were recommended to reach the standard quantity.After one year,their nutrition status and diet structure were remeasured.Results: Under the nutrition doctor' direction,they could change unreasonable diet structure,and received a balanced and enough diet.At last,their nutrition status had been improved.Conclusion: It is feasible to adjust the medical students' diet structure and improve their nutrition and health status by the means of nutrition education.

Objective To investigate the changes of myocardial combination enzyme in the early stages of gunshot wound of dogs in seawater for exploring early treatment protocol. Methods Fourteen conventional Beagles models undergoing gunshot wound in seawater were used along with another 3 dogs receiving the wound on land to serve as control. After the dogs were rescued from the seawater, the origin of blood coming from central venous pressure tube was measured (33.75?25.29) min before and after injured for indexes such as alkaline aminotransferase (ALT) , aspartate aminotransferase (AST) , creatine kinase (CK) , creatine kinase isoenzymes - MB (CK -MB) , lactate dehydrogenase (LDH) and adenosine monophosphate (AMY) in the early stages of wound, followed by statistical analysis of the results. Results There was an increase in blood CK (P0.05). As compared with the control, the experimental group in seawater was observed to have significant difference in blood AMY (P 0.05). But it was noted that CK, CK - MB , LDH , AST and ALT contents tended to increase. Conclusion The functions of respiratory, heart, liver and kidney were injured and restrained in Beagle dogs undergoing the firearms wound in natural seawater. The immediate diagnosis and treatment of the organism as well as the out - of - wound - tract injury shoud do while the firearms primal wound tract were treated in the early stages of gunshot wound.

Objective To discuss the laws of treatment of chronic fatigue syndrome(CFS) by acupuncture and moxibustion.Methods The literature between 1998-2008 were searched by means of electronic retrieval.The database was established by Epidata 3.1.Applying Excel 2000 to analyze the literatures on the following aspects:the diagnosis of CFS in TCM,the therapeutic methods and manipulations,acupoint selection,course of treatment and therapeutic effects of CFS treated by acupuncture and moxibustion.Results Of the 78 literatures enrolled,50 diagnozed CFS in TCM as vacuity taxation or vacuity detriment,22 as depression pattern.53 identified disease and administered treatment with settled prescription of acupoints,21 identified patterns and combined points.84 body points and 18 auricular points were founded in the prescriptions involving the points of 14 meridians and extra points.28 were treated with one kind of acupuncture and moxibustion methods,50 with more than one kind of acupuncture and moxibustion methods.48 were acupunctured by means of filiform needle and 8 cooperated with electro-acupuncture,18 by means of moxibustion,18 cupping,9 pricking blood,6 auricular therapy,4 dermal needle,3 point-injection,2 embedding therapy and 2 acupoint application with herbal medicine.44 were treated once a day,21 once every other day.The means of total treatment times were 22?11.52 had the total efficiency above 90%,and 17 between 80%～90%.Conclustion Among all kinds of acupuncture and moxibustion methods in treating CFS,filiform needle,electro-acupuncture,moixbustion and cupping were mostly used,and generally more than one kinds of methods were cooperated.The points selected mainly in UB meridian of foot Taiyang,Ren meridian and Du meridian.The points used in high frequency are as follows:ST36,SP6,UB23,DU20,UB15,UB18,UB20,REN4,REN6 and LIV3,generally it needed comparatively long time of treatment to acquire better therapeutic effects.

Objective:To study attitude of medical students to dying and death in order to improve medical education.Method:451 medical students were investigated with self-made questionnaire.Results:Having encountered death of others,having dying family members had great influence on attitude to death.The acceptance of family to death and dying,and culture viewpoint on death had also significant influence.