Objective To investigate the influence of donepezil hydrochloride and hyperbaric oxygen on cognitive function in patients with vascular dementia.Methods 61 cases of patients with vascular dementia were chosen to research,and were divided into research group(31 cases)and control group(30 cases),the control group was treated with piracetam,the research group was treated with donepezil hydrochloride and hyperbaric oxygen,after 12 weeks of treatment congnitive function,daily life quality and adverse reaction between two group were compared. Results The ADL score and MMSE score between the two group had no significant difference before treatment(all P <0.05).The MMSE score of the research group were higher than the control group[(18.71 ±3.29)points vs (15.83 ±3.45)points]after treatment,the difference was statistically significant (t =3.337,P <0.05).The ADL score of the research group were lower than control group[(37.12 ±7.13)points vs (42.53 ±6.74)points],the difference was statistically significant (t =3.026,P <0.05).4 cases of mild to moderate earache and 1 case of mild diarrhea occurred in the research group,and 1 case of upper abdominal pain occurred in the control group,there was no significant difference in the incidence of adverse reactions between the two groups (χ2 =0.803,P >0.05 ). Conclusion Donepezil hydrochloride combined with hyperbaric oxygen could effectively improve the cognitive function of patients with vascular dementia,and with good safety.

China ; Humans ; Respiratory System ; pathology

Alloys ; Cobalt ; Humans ; Lung ; pathology ; Lung Diseases ; diagnosis ; pathology ; Tungsten

OBJECTIVE To explore the role of gecko crude peptides (GCPs) in the proliferation, apoptosis, migration and lymphangiogenesis of human hepatocellular carcinoma cells (HepG2) and human lymphaticendothelial cells (HLECs) in vitro. METHODS The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the anti- proliferative effect of GCPs and siRNA-VEGF-C on HepG2 cells, Hoechst 33258 staining and flow cytometry were performed to analyze cycle and apoptosis. The migration and invasion ability of cells were assayed by transwell chamber experiment and wound-healing assay. The protein and mRNA expressions of vascular endo?thelial growth factor-C (VEGF-C) and CXC chemokine receptor-4 (CXCR4) were detected by q-PCR, immunofluorescence, Western blot. The protein expressions of the extracellular signal regulated kinase (ERKI/2), c-Jun N-terminal kinase (JNK), p38-mitogen activated protein kinases (p38 MAPK), serine/threonine kinase (Akt) and phosphatidylinositol- 3- kinase (PI3K) were detected by western blot. The anti-lymphangiogenesis effect of GCPs on the HLECs was analyzed using an in vitro tube-formation assay. The protein and mRNA expressions of vascular endothelial growth factor receptor-3 (VEGFR-3) and stromal cell-derived factor-1 (SDF-1) were detected by q-PCR, Western blot. RESULTS GCPs and siRNA-VEGF-C inhibited HepG2 proliferation, invasion and migration, and the most obvious inhibitory effect was both synergistic effects. Thus, GCPs suppressed HLECs proliferation, migration and tube-like structure formationin a dose- dependent manner, and had inhibitory effect of tumor- induced lymphangiogenesis in vitro. Additionally, we found that GCPs and siRNA- VEGF- C decreased the expressions of MMP-2, MMP-9, VEGF-C, CXCR4, phospho-ERK1/2, phospho-P38, phospho-JNK and PI3K in HepG2 cells. Moreover, GCPs had a dose-dependent depressive effecton the expressions of VEGFR- 3, SDF- 1 in HLECs. CONCLUSION The low expression of VEGF- C mediated by siRNA-VEGF-C and GCPs inhibit tumor proliferation, invasion and migrationby suppressing the MAPK signaling pathway through reduced levels of VEGF-C, and GCPs inhibit tumor lymphangiogenesis by suppressing the CXCR4/SDF-1 signaling pathway through suppressed VEGF-C/VEGFR-3.

Bile Duct Neoplasms ; diagnosis ; Bile Ducts, Intrahepatic ; pathology ; Cholangiocarcinoma ; diagnosis ; Humans ; In Situ Hybridization, Fluorescence

Anticoagulants ; poisoning ; Humans ; Male ; Middle Aged ; Rodenticides ; poisoning

Animals ; CHO Cells ; Cricetulus ; Doxycycline ; pharmacology ; Immunohistochemistry ; Ki-67 Antigen ; genetics ; metabolism ; Plasmids ; Quality Control ; Staining and Labeling ; methods ; Transfection

Diagnosis, Differential ; Humans ; Neoplasms, Glandular and Epithelial ; diagnosis ; Thymus Neoplasms ; diagnosis

Radiotracer techniques were employed to characterize 65Zn adsorption and desorption in root-cell-wall of hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) species of Sedum alfredii Hance. The results indicated that at the end ofa 30 min short time radioisotope loading period, comparable amounts of 65Zn were accumulated in the roots of the two ecotypes Sedum alfredii, whereas 2.1-fold more 65Zn remains in NHE root after 45-min desorption. At the end of 60 min uptake period, no difference of 65Zn accumulation was observed in undesorbed root-cell-wall of Sedum alfredii. However, 3.0-fold more 65Zn accumulated in desorbed root-cell-wall ofNHE. Zn2+ binding in root-cell-wall preparations of NHE was greater than that in HE under high Zn2+ concentration. All these results suggested that root-cell-wall of the two ecotypes Sedum alfredii had the same ability to adsorb Zn2+, whereas the desorption characteristics were different, and with most of 65Zn binding on root of HE being available for loading into the xylem, as a result, more 65Zn was translocated to the shoot.

OBJECTIVETo observe the therapeutic effect of acupoint-injection combined with manipulation training on child cerebral palsy.

METHODSThe patients in the treatment group were treated with acupoint-injection combined with manipulation training and activator of brain cells, and the control group with simple manipulation training and activator of brain cells. Their therapeutic effects were compared.

RESULTSAfter treatment of 3-4 courses, the total effective rate of 92.3% in the treatment group was superior to 75.9% in the control group (P < 0.05).

CONCLUSIONThe therapeutic effect of acupoint-injection combined with manipulation training on child cerebral palsy is better than that of the simple manipulation training.

Acupuncture Points ; Acupuncture Therapy ; methods ; Cerebral Palsy ; therapy ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Injections ; Male ; Musculoskeletal Manipulations ; methods