Objective: To study the inhibition effect of survivin antisense oligodeoxynucleotide (ASODN)on apoptosis and invasion of hilar cholangiocarcinoma cell line FRH-0201. Methods: ASODN was transfected into cholangiocarcinoma cell line FRH-0201 by LipofectamineTM2000. The level of MMP-2 and TIMP-2 in the supernatant was detected by enzyme linked immunosorbent assay (ELISA). The effect of ASODN transfection was observed by Transwell cell culture chamber. Results: The cell proliferation was inhibited in the ASODN group. The expression of MMP-2 in ASODN group was lower than those in the SODN and blank group (P

Objective:To assess the reliability and validity of the Self-rating Internet-using Scale.Methods:A sample of 1240 middle school students was selected for pilot evaluation of the scale.A test-retest study was conducted to evaluate the reliability among 130 middle school students.The data was analyzed by descriptive analysis method.Results:It showed that the test-retest reliability was 0.74,and the Cronbach's ? coefficient was 0.86.Factor analysis extracted 8 factors,with factor loadings between 0.382 and 0.811 and a cumulative contribution rate of 60.1%.When the cut-off point of the scale for diagnosis was 91(x+1.645s),the sensitivity was 26.9%,the specificity was 94.0% the positive rate of internet addiction disorder(IAD) was 6.8%.The positive rate of IAD in boys was significantly higher than that in girls(9.9% vs.4.2%,P

With the growing comprehensive national strength and international position,the international exchanges in medical health care field become actively and frequently,in which the medical associations play a proactive and facilitating role.The importance of foreign affairs of medical associations emerges.The management of foreign affairs is an important area of management.The foreign affairs must be in compliance with national policy to guide,regulate and dispose issues and problems.The management of foreign affairs of medical associations is one of the tasks of civil diplomacy and is also key component of civil diplomacy of Chinese characteristics which serves for national holistic diplomacy policy.The author is a project manager dealing with foreign affairs in Chinese Medical Association (CMA).Therefore,the author plans to analyze current international exchanges of CMA so as to explore certain enlightenments for peers.The paper is written based on international exchanges practice by CMA and analysis of typical cases,so as to raises some thoughts about how to improve the work of management of foreign affairs of medical associations.

ObjectiveTo explore the content of isorhynchophylline in different medicinal parts of Ramulus Uncariae Cum Uncis.MethodsHPLC was adopted to determine 8 batches of different Ramulus Uncariae Cum Uncis of different origin and different medicinal parts. The Waters Symmetry C18 color(4.6 mm×250 mm, 5μm) was used with mobile phase of methanol -0.01 mol/L ammonium acetate buffer(pH 8.0)(60∶40), column temperature of 25℃ , 20μl sample volume, velocity of 1.0 ml/min, and detection wavelength of 246 nm. ResultsIsorhynchophylline can be detected in all 8 batchs of Ramulus Uncariae Cum Uncis in different regions, and different content were found among different origins. The content of isorhynchophylline in different parts of the same origin showed a decreasing sequence of the rhabd, stem with hook, stem without hook, and twig without hook and leaves.ConclusionMost of medicinal part of Ramulus Uncariae Cum Uncis contains isorhynchophylline, which provide a lab basis for exploring medicinal parts of this herbal medicine.

Objective To discuss the diagnostic values of transrectal ultrasound-guided puncture biopsy in prostate carcinoma(PCa).Methods A total of 238 patients with suspected PCa received tran-srectal ultrasound-guided puncture biopsy.Results Based on the results of biopsy pathology,88 cases (37.0%)were diagnosed as PCa,105 cases(44.1%)were benign prostate hyperplasia,15 cases (6.3%) were low-grade prostatic intraepithelial neoplasm,17 cases(7.1%)were atypical hyperplasia,8 cases(3. 4%)were adenomatoid tumor,and 5 cases(2.1%)were granulomatous prostatitis.Conclusion Tran-srectal ultrasound-guided puncture biopsy is accurate and safe.It is an effective method in diagnosing pros-tatic carcinoma.

Objective To explore the key parameters of eliminating the interference of chyle by adopting the high-speed centrifu-gation technique.Methods To establish the chylemia by adding chylomicrons to background serum,centrifuge the same model un-der five different parameters,compare the biochemical indicators of lower serum with background by matched t-test,and to analyze the relative bias of the experimental group with statistical differences.Results Compared with the background serum,for ALT, AST,TP,ALB,ALP,GGT and Fe,the correlation coefficient was higher and the differences had no statistical significance(P >0.01 )when centrifuging for 20 min by RCF 29 703 g;for CK and CHE,the correlation coefficient was higher and the differences had no statistical significance(P >0.01)when centrifuging for 15 min by 20 672 g;for Zn,the correlation coefficient was higher and the differences had no statistical significance(P >0.01)when centrifuging for 5 min by 9 168 g.For Glu,LDH,CRE,Urea,UA,Ca and P,all the experimental groups had statistically significant differences(P <0.01),but the relative bias in each group were within the acceptable range.For Ca and P,the correlation coefficient was higher when centrifuging for 15 min by 20 672 g.Conclusion It is recommended to eliminate the interference of chyle on ALT,AST,TP,ALB,ALP,GGT,Fe,Glu,LDH,CRE,Urea and UA by u-sing the centrifugal parameters(29 703 g,20 min),on CK and CHE by using the centrifugal parameters(20 672 g,15 minutes)and on Zn,Ca and P by using the centrifugal parameters(9 168 g,5 minutes).

Breast cancer related lymphedema is the accumulation of interstitial fluid due to the lymphatic fluid load exceeds its transport ability as a consequence of the lesion of lymphatic structure.It mainly presents as swelling of the affected limb,a feeling of heaviness or tightness in the limb,aching and restricted limb motion.Its incidence is related to surgery,chemical therapy,obesity,hypertention as well as the alteration of particular genes.To prevent the occurrence and exacerbation of lymphedema,patients should avoid the overuse of the affected limb.While limb exercise step by step can solve part of this problem,but not all,doctor should also take both the patterns of exercise and risk factors that patients expose into consideration,then make a individualized exercise plan,evaluate the condition precisely,in order to achieve the best treatment purposes.

Informatics of traditional Chinese medicine is an emerging subject.This dissertation analyzes the connotation and scope of the study of traditional Chinese medicine informatics.Through investigating the demand for personnel talents of Chinese medicine hospital and Information Institute of TCM,we comment on with what theoretical basis and practical abilities the TCM information personnel talents should possess.It also proposes the training mode and optimization measures of TCM Informatics education.

Objective:To evaluate the clinical efficacy of Shiwei-Jinghuang Ointment combined with Chinese herbal external washing prescription in the treatment of acute paronychia. Methods:A total of 126 patients with acute paronychia in Xiyuan Hospital of China Academy of Chinese Medical Sciences from January to December 2019, who met the inclusion criteria, were divided into 3 groups according to the random number table method (42 in each group). The treatment group was treated with Shiwei-Jinghuang Ointment combined with Chinese herbal external washing prescription. The control group 1 and group 2 were simply applied with Chinese herbal external washing prescription and mupirocin ointment, respectively. All groups were treated for 2 weeks and followed up 2 months. Serum CRP level was determined by ELISA method, and neutrophil percentage (NEU%) was measured by automatic hematology analyzer. The subsided time of red swelling and inflammatory exudation in nail groove of the paronychia patients were observed and recorded. Results:The total effective rate was 90.5% (38/42) in the treatment group, 71.4% (30/42) in the control group 1 and 66.7% (28/42) in the control group 2. The statistical difference were significant among three groups ( χ2=7.350, P=0.025). After treatment, the serum CRP level and NEU% in three groups were decreased ( P<0.01), and the serum CRP and NEU% level in treatment group were significantly lower than those in the control group 1 and group 2 ( F values were 6.482, 33.619, respectively, all Ps<0.001). After 2 months of follow-up, the incidences of chronic paronychia were 50.0% (6/12) and 57.1% (8/14) in control group 1 and group 2, respectively, but no chronic paronychia occurred in the treatment group. The difference among three groups was statistically significant ( χ2=9.355, P=0.009). Conclusion:The combination of Shiwei-Jinghuang Ointment and Chinese herbal external washing prescription can promote paronychia red swelling, reduce the inflammatory exudation time and the incidence of chronic paronychia, effectively improve the in vivo decrease inflammation index and clinical efficacy.

Therapies for uveitis include drug treatment and surgery.Medical therapies including proper administration of corticos- teroids,immunosuppressive drugs,nonsteroidal anti-inflammtion drugs and anti-infective drugs are important in management of the in- traocular inflammation and decreasing the risk of complications,furthermore,are the basis for surgical treatment of uveitis.