Informatics of traditional Chinese medicine is an emerging subject.This dissertation analyzes the connotation and scope of the study of traditional Chinese medicine informatics.Through investigating the demand for personnel talents of Chinese medicine hospital and Information Institute of TCM,we comment on with what theoretical basis and practical abilities the TCM information personnel talents should possess.It also proposes the training mode and optimization measures of TCM Informatics education.

Combined therapy consisting of radiotherapy,chemotherapy and palliative surgery is the primary management for locally advanced pancreatic cancer,with the purpose of prolonging survival and improving life quality.Gemcitabine is the standard chemotherapy at present.Gemcitabine-based combinations show a definite effect,and the combination with targeted drugs also has a certain degree of efficacy.The development of new chemotherapy drugs and targeted drugs provides more ways for the teatment of locally advanced pancreatic cancer.

Objective To discuss the diagnostic values of transrectal ultrasound-guided puncture biopsy in prostate carcinoma(PCa).Methods A total of 238 patients with suspected PCa received tran-srectal ultrasound-guided puncture biopsy.Results Based on the results of biopsy pathology,88 cases (37.0%)were diagnosed as PCa,105 cases(44.1%)were benign prostate hyperplasia,15 cases (6.3%) were low-grade prostatic intraepithelial neoplasm,17 cases(7.1%)were atypical hyperplasia,8 cases(3. 4%)were adenomatoid tumor,and 5 cases(2.1%)were granulomatous prostatitis.Conclusion Tran-srectal ultrasound-guided puncture biopsy is accurate and safe.It is an effective method in diagnosing pros-tatic carcinoma.

Objective To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of glutamine synthetase (GS) of cultured rat retinal Müller cells in high glucose environment in vitro.Methods Mtüller cells were isolated from retinas of 10 Sprague-Dawley rats at postnatal day three to five by trypsin digestion,and were randomly divided into six groups,including normal control group,high glucose group,high glucose +5 U/ml rhEPO group,high glucose+ 10 U/ml rhEPO group,high glucose+ 20 U/ml rhEPO group,high glucose+40 U/ml rhEPO groups.After 48 hours,the apoptosis of retinal Müller cells were assayed by terminal transferase-mediated DNA end labelling assay,and the expression levels of GS protein were detected with semi-quantitative immunocytochemistry.Results Compared with the normal control group,the cell viability and GS protein were reduced while the cell death increased in Müller cells cultured in high glucose,the difference was statistically significant (t =27.4,P ＜ 0.01).Compared with the high glucose group,rhEPO treatment reduced the apoptotic Müller cells (t=857.2,2 374.6,2 473.2,2 537.7; P＜0.01),induced the expression of GS proteins (t=3.2,18.0,22.5,26.4; P＜0.05).Conclusions rhEPO can protect Müller cells from apoptosis under high glucose condition.The mechanism may be related to its function to up-regulate the GS protein expression,promote glutamic acid cycle,and reduce the excitotoxicity effects of high concentration of glutamate.

Intracellular calcium is one of the crucial signalings that modulate various physiological and pathological processes.Abnormal calcium signaling is closely associated with invasion and migration of neoplasms.Calcium regulates focal adhesion turnover,cytoskeletal reorganization and other processes about tumor cell movement through contacting with multiple downstream proteins,such as calpain,PYK2 and S100 protein family.Several calcium entry channels including store-operated calcium channels and transient receptor potential channels participate in the invasion and migration of tumor cells.Pathological calcium signaling may be a potential target in anti-invasion and anti-migration therapy.

Leptin,a proteohormone primarily made by adipocytes,directly interacts with the hypothalamus for energy balance regulation.It plays an important role in the morbility of obesity.The soluble leptin receptor(sOb-R) is one of the leptin receptor isoforms.The examination of the sOb-R concentration is valuable because of its function in the regulation of leptin concentration.Leptin shows a great potential as a childhood obesity biomarker,and its concentration could be used in assessing the responsiveness of obese children to intervention programs.

Taking eugenics,family planning and reproductive human-assisted projects for instance,the article discusses the conflicts between modern biotechnology and the contemporary ethics.It distinguishes the concepts of life quality and that of health,and advocates that the modern medicine should conduct to be in pursuit of the high quality of life.Finally,it puts forward that we should upgrade the quality of individual's life in the circumstance of a stable and integrated communal ethics.Biotechnology may be developed immeasurably,but should be used restricted and morally.

The donors' complete autonomy and voluntariness for donation is the core of ethical acceptability of living organ transplantation.Deep in the donors' heart,the awareness of self-respect and the altruism are two contradictory tendencies both in psychology and behavior.As the existence of the tension of self-respect awareness and altruism,it becomes particularly difficult and complex for the living organ donors to make autonomous decisions.This paper analyzed the tension of self-respect awareness and altruism,discussed the emotional and ethical contradictory,and proposed some suggestions based on this,hoping to provide a good internal environment and external support for the potential donors to make reasonable free decisions and guarantee the ethics of living organ transplantation.

The application of transesophageal echocardiography (TEE) in perioperative monitoring was introduced,and its involvement in non-cardiac surgery was described.The advantages and disadvantages of TEE were analyzed when applied to cardiac and non-cardiac operations for congenital heart disease,coronary disease,vascular heart disease,aorta disease and etc.The abuse of vasoactive drug or fluid is avoided due to subjective evaluation on cardiac function and volume load.TEE facilitates the anesthetist in diagnosis and evaluation of the patient with heart diseases,and the surgeon in immediate assessment of the operation,which can be used for real-time monitoring of cardiac function and hemodynamics.