1.Anti-mouse CD122 antibody promotes the hematopoietic repopulating capacity of cord blood CD34⁺ cells in NOD/SCID mice.
Men-Yao SHENG ; Hui SHI ; Wen XING ; Wen-Jun WANG ; Xiao-Hui SI ; Jie BAI ; Wei-Ping YUAN ; Yuan ZHOU ; Feng-Chun YANG
Journal of Experimental Hematology 2014;22(6):1673-1677
The study was aimed to investigate the effect of anti-mouse CD122 antibody on the hematopoietic repopulating capacity of cord blood CD34⁺ cells in a humanized murine model-non obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. After sublethal irradiation with γ-ray, NOD/SCID mice were intraperitoneally injected with 200 µg mouse isotype control antibody or anti-mouse CD122 antibody. Human cord blood CD34⁺ cells or phosphate-buffered saline (PBS) were injected via the tail vein at 6-8 hours later. Cohort of the mice injected with anti-mice CD122 antibody or control antibody alone were sacrificed at different time point (at week 2, 3, and 4 weeks) after the injection, and the percentage of NK cells in the peripheral blood was analyzed by flow cytometry. To evaluate the effect of anti-mouse CD122 antibody on the repopulating capacity of cord blood CD34⁺ cells in the recipient mice, phenotype analysis was performed in the bone marrow at 6 and 8 weeks after the transplantation. The results showed that the proportion of NK cells in the peripheral blood were (4.6 ± 0.6)% and (5.7 ± 1.7)% at week 2 and 3 after anti-CD122 antibody injection respectively,which decreased by 60%, compared with the mice injected with isotype control antibody. After 6 and 8 weeks of cord blood CD34⁺ cell transplantation,the percentage of human CD45⁺ in the bone marrow of the recipient mice treated with anti-mice CD122 antibody was (63.0 ± 12.2)% and (53.2 ± 16.3)%,respectively,which were dramatically higher than that in the mice treated with isotype control antibody (7.7 ± 3.6)% and (6.1 ± 2.4)%. Moreover,at 8 weeks after transplantation,human CD34⁺ cells appeared significantly in the recipients treated with anti-CD122 antibody. It is concluded that the anti-mouse CD122 antibody enhances the hematopoietic repopulating capacity of cord blood CD34⁺ cells in the NOD/SCID mice through decreasing the proportion of NK cells.
Animals
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Antibodies
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immunology
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Antigens, CD34
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Bone Marrow
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Cord Blood Stem Cell Transplantation
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Fetal Blood
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immunology
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Hematopoietic Stem Cell Transplantation
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Hematopoietic System
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cytology
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immunology
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Humans
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Interleukin-2 Receptor beta Subunit
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immunology
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Killer Cells, Natural
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Mice
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Mice, Inbred NOD
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Mice, SCID
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Transplantation, Heterologous
2.Consensus statement on human immunodeficiency virus pre-exposure prophylaxis in China.
Jun-Jie XU ; Xiao-Jie HUANG ; Xin-Chao LIU ; Li-Ming WANG ; Yao-Kai CHEN ; Hui WANG ; Fu-Jie ZHANG ; Hao WU ; Tai-Sheng LI ; Meng-Jie HAN ; Fang ZHAO ; Hai-Bo DING ; Jun-Yi DUAN ; Gen-Shen SHENG ; Hong SHANG
Chinese Medical Journal 2020;133(23):2840-2846