Chemical investigation of the plant Tristemma hirtum P. Beauv (Melastomataceae) resulted to the isolation of a new flavonol glycoside named quercetin-7-O-α-D-arabinofuranoside (1), together with nine known compounds including 3′-hexadecanoyl-2′-(9aZ)-tetradecanoyl-glycerol 1′-O-[β-D-galactopyranosyl-(1″ → 6″)-α-D-galactopyranoside] (2), arjunolic acid (3), β-sitosterol-3-O-β-D-glucopyranoside (4), terminolic acid (5), quercetin (6), asiatic acid (7), maslinic acid (8), 1β-O-galloylpedunculagin (9) and 6-hydroxyapigenin 7-O-β-D-glucopyranoside (10) from the methanol extract using normal and reversed phase column chromatography. The structures of these compounds were determined by comprehensive interpretation of their spectral data mainly including 1D- 2D-NMR (¹H-¹H COSY, HSQC, and HMBC) spectroscopic and ESI-TOF-MS mass spectrometric analysis.
Chromatography ; Melastomataceae ; Methanol ; Plants ; Quercetin

Phytochemical studies of the the herb Sarcopyramis bodinieri var. delicate (Melastomataceae) have been carried out. The compounds were separated by repeated D101 macroporous adsorption resin column combined with Sephadex LH-20, ODS, and silica gel chromatgrophy. The structures were identified on the basis of extensive spectroscopic data analysis, and by comparison of their spectral data with those reported. Eight flavonoid compounds isolated from the ethyl acetate extract was identified as isorhamnetin (1), quercetin (2), isorhamnetin-3-O-beta-D-glucopyranoside (3), quercetin-3-O-beta-D-glucopyranoside (4), isorhamnetin-3-O-(6"-acetyl)-beta-D-glucopyranoside (5), isorhamnetin-3-O-(2"-acetyl)-beta-D-glucopyranoside (6), quercetin-3-O-(6"-acetyl)-beta-D-glucopyranoside (7), and quercetin- 3-O-(6"-O-E-p-coumaroyl)-beta-D-glucopyranoside (8). All of the compounds were separated from the genus of Sarcopyramis for the first time.
Flavonoids ; analysis ; isolation & purification ; Magnetic Resonance Spectroscopy ; Melastomataceae ; chemistry ; Spectrometry, Mass, Electrospray Ionization

There are 63 species Melastomataceae plants in 17 genus, which widely distribute along Yangtze River and the south of China ranging from Tibet autonomous region to Taiwan province. They used as herb medicine in China. A large part of the Melastomataceae plants have bitter, pungent and sweet taste. The meridian distribution of them is liver, spleen and stomach, they have many functions such as "cure rheumatism", "clear heat" and "detoxication", "regulate the flow of qi and alleviate pain", "diuresis and detumescence", "activate the blood and eliminate stasis". Melastomataceae plants not only have exact medical value, but also have abundant resource. So it has very bright perspective of exploitation and utilization.
Adult ; Child ; China ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Humans ; Male ; Melastomataceae ; chemistry ; classification ; Phytotherapy ; Pregnancy

OBJECTIVETo establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb.

METHODThe RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm.

RESULTThe content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations.

CONCLUSIONThe method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum.

China ; Chromatography, High Pressure Liquid ; methods ; Ecosystem ; Gallic Acid ; analysis ; Melastomataceae ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control

OBJECTIVETo investigate the chemical constituents of Chinese herb Melastoma dodecandrum.

METHODThe chemical constituents were isolated by solvent extraction and chromatography. Their structure were identified on the basis of physic-chemical constants and specral data.

RESULTFive compounds were isolated and identified as hexacosanoic acid (I), quercetin(II), avicularin(III), gallic acid (IV), kaempferol(V).

CONCLUSIONThe above-mentioned compounds were separated from the plant for the first time.

Fatty Acids ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Gallic Acid ; chemistry ; isolation & purification ; Kaempferols ; chemistry ; isolation & purification ; Melastomataceae ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; chemistry ; isolation & purification

OBJECTIVETo study the chemical constituents of the roots of Osbeckia opipara.

METHODRepeated column chromatography over silica gel, RP-18 and Sephadex LH-20, and preparative thin layer chromatography(PTLC) were used to isolate the compounds, whose structures were determined by spectroscopic methods by direct comparing spectral data with those reported references.

RESULTFrom the MeOH extract of the roots O. opipara, twelve compounds were isolated and identified as follows: lasiodiplodin (1) , de-O-methyllasiodiplodin (2), 2, 3- dihydro-2-hydroxy-2, 4-dimethyl-5-trans-propenylfuran-3-one (3), integracin (4), 5alpha, 8alpha-epidioxy-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (5), 3, 3', 4'-tri-O-methylellagic acid (6), 5-hydroxymethyl furaldehyde (7), vomifolio (8) , betulintic acid (9), 2alpha-hydroxyursolic acid (10), (24R)-stigmast-4-ene-3-one (11), and eugenitin (12).

CONCLUSIONCompounds 1-12 were isolated from O. opipara for the first time.

Cholestenones ; chemistry ; isolation & purification ; Fermentation ; Fruiting Bodies, Fungal ; chemistry ; Melastomataceae ; chemistry ; Molecular Structure ; Plant Extracts ; chemistry ; Plant Roots ; chemistry ; Spectrum Analysis ; Triterpenes ; chemistry ; isolation & purification