OBJECTIVE: To study the cytotoxicity of recombinate toxin MSH-Ang to Hep-2. METHOD: The depurated MSH-Ang were applied in cytotoxicity experiment, and the growth inhibiting action to laryngeal carcinoma cell Hep-2 were observed. RESULT: Recombination protein inhibited the growth of laryngeal carcinoma cell Hep-2, and its inhibiting action enhanced and corpuscular mortality rate increased along with the concentration increasing. CONCLUSION Recombinant toxin MSH-Ang can not only take special effect in tumors with high MSHR, but also target to many other popular tumors.
Angiopoietins ; genetics ; pharmacology ; Cell Line, Tumor ; Genetic Engineering ; Humans ; Laryngeal Neoplasms ; Melanocyte-Stimulating Hormones ; genetics ; pharmacology ; Recombination, Genetic

The current study was designed to examine the effects of intracerebroventricular injections of SHU9119 [a nonselective melanocortin receptor (McR) antagonist] and MCL0020 (a selective McR antagonist) on the serotonin-induced eating and drinking responses of broiler cockerels deprived of food for 24 h (FD24). For Experiment 1, the chickens were intracerebroventricularly injected with 2.5, 5, and 10 microg serotonin. In Experiment 2, the chickens received 2 nmol SHU9119 before being injected with 10 microg serotonin. For Experiment 3, the chickens were given 10 microg serotonin after receiving 2 nmol MCL0020, and the level of food and water intake was determined 3 h post-injection. Results of this study showed that serotonin decreased food intake but increased water intake among the FD24 broiler cockerels and that these effects occurred in a dose-dependent manner. The inhibitory effect of serotonin on food intake was significantly attenuated by pretreatment with SHU9119 and MCL0020. However, the stimulatory effect of serotonin on water intake was not altered by this pretreatment. These results suggest that serotonin hypophagia and hyperdipsia were mediated by different mechanisms in the central nervous system, and that serotonin required downstream activation of McRs to promote hypophagia but not hyperdipsia in the FD24 chickens.
Animals ; Chickens ; Dose-Response Relationship, Drug ; Drinking Behavior/*drug effects ; Feeding Behavior/*drug effects ; Food Deprivation ; Injections, Intraventricular/veterinary ; Male ; Melanocyte-Stimulating Hormones/*pharmacology ; Oligopeptides/*pharmacology ; Receptor, Melanocortin, Type 3/*antagonists & inhibitors ; Receptor, Melanocortin, Type 4/*antagonists & inhibitors ; Serotonin/pharmacology

OBJECTIVETo observe the effect of Tribulus terrestris extract on melanocyte stimulating hormone (MSH) expression in C57BL/6J mouse hair follicles, and investigate the role of Tribulus terrestris extract in activation, proliferation, epidermal migration of dormant hair follicle melanocytes.

METHODSThe aqueous extract of Tribulus terrestris was administered orally in specific pathogen-free C57BL/6J mouse at the daily dose equivalent to 1 g/1 kg in adult human, and the expression and distribution of MSH in the mouse hair follicles was observed with immunohistochemistry.

RESULTSThe positivity rate of MSH expression in the hair follicle melanocytes was 75% in mice treated with the extract, significantly higher than the rate of only 18.75% in the control group (P<0.01).

CONCLUSIONThe aqueous extract of Tribulus terrestris can significantly increase MSH expression in the hair follicle melanocytes by activating tyrosinase activity and promoting melanocyte proliferation, melanine synthesis, and epidermal migration of dormant melanocytes.

Administration, Oral ; Animals ; Cell Proliferation ; drug effects ; Female ; Hair Follicle ; cytology ; drug effects ; metabolism ; Immunohistochemistry ; Melanocyte-Stimulating Hormones ; biosynthesis ; Melanocytes ; cytology ; drug effects ; metabolism ; Mice ; Mice, Inbred C57BL ; Plant Extracts ; administration & dosage ; pharmacology ; Random Allocation ; Tribulus ; chemistry